Abstract:
BACKGROUND:Hepatocellular carcinoma is a common cancer, ranking third in cancer-associated deaths. An important cause of cancer patients' mortality is metastasis. At the start of metastasis progression, there is an epithelial-mesenchymal transition, characterized by matrix degradation, junction reductions and vessels formation. HuH-7 is a cell line used in research as an in vitro model for hepatocellular carcinoma. It is known that two-dimensional growth reflects tumor characteristics poorly. In contrast, three-dimensional cultures provide a better approach to the study of tumorigenic potential. The purpose of this work was to mimic a three-dimensional environment in order to assess gene expression of some epithelial-mesenchymal transition and metastasis progression markers in HuH-7 cells and compare them with traditional two-dimensional culture model. METHODS:HuH-7 cells were encapsulated in sodium alginate (three-dimensional model) to be compared with cells grown in two-dimensional flasks. After 4 days in culture, gene expression of Matrix metallopeptidase 9, Occludin, p65, Intercellular adhesion molecule 1 and Vascular endothelial growth factor A was analyzed by qPCR and cytoskeleton assessment was performed by rhodamine-phalloidin staining. RESULTS:Differences were found in gene expression, with a high increment of Matrix metallopeptidase 9 and Occludin reduction. The cytoskeleton morphology also showed differences, with a cytoplasm restricted only near the nuclei in the three-dimensional model. CONCLUSIONS:This work shows the effects of using sodium alginate capsules as a three-dimensional model to the study of HuH-7. Cells in this 3D system show key markers of epithelial-mesenchymal transition, such as Matrix metallopeptidase 9 overexpression and Occludin down-regulation.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Angiolini VA,Cruz CU,López ML,Simon L,Matte Udoi
10.1186/s12935-016-0370-xsubject
Has Abstractpub_date
2017-01-04 00:00:00pages
5issn
1475-2867pii
370journal_volume
17pub_type
杂志文章abstract:Background:A number of JmjC domain-containing histone demethylases have been identified and biochemically characterized in mammalian models and humans. JMJD2A is a transcriptional co-factor and enzyme that catalyzes the demethylation of histone H3 lysine 9 and 36 (H3K9 and H3K36). Here in this study, we reported the ro...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01177-z
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journal_title:Cancer cell international
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journal_title:Cancer cell international
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journal_title:Cancer cell international
pub_type: 杂志文章,评审
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journal_title:Cancer cell international
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journal_title:Cancer cell international
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journal_title:Cancer cell international
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01700-2
更新日期:2021-01-06 00:00:00
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doi:10.1186/1475-2867-10-17
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pub_type: 杂志文章
doi:10.1186/s12935-020-01207-w
更新日期:2020-04-26 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12935-020-01616-x
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0453-3
更新日期:2017-09-26 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0827-9
更新日期:2019-04-23 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0968-x
更新日期:2019-11-27 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章,评审
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journal_title:Cancer cell international
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doi:10.1186/s12935-016-0352-z
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2020-05-29 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0628-6
更新日期:2018-09-03 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-8-15
更新日期:2008-11-03 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0693-x
更新日期:2018-12-04 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-14-6
更新日期:2014-01-23 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-14-38
更新日期:2014-05-06 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章,评审
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更新日期:2017-10-30 00:00:00
abstract::BACKGROUND: The aim of this work was to investigate in vitro the putative role of EGR-1 in the growth of glioma cells. EGR-1 expression was examined during the early passages in vitro of 17 primary cell lines grown from 3 grade III and from 14 grade IV malignant astrocytoma explants. The explanted tumors were genetica...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-4-1
更新日期:2004-01-07 00:00:00
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pub_type: 杂志文章
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更新日期:2019-02-14 00:00:00