Abstract:
BACKGROUND:The repertoire of free-living protozoa in contact lens solutions is poorly known despite the fact that such protozoa may act as direct pathogens and may harbor intra-cellular pathogens. METHODS:Between 2009 and 2014, the contact lens solutions collected from patients presenting at our Ophthalmology Department for clinically suspected keratitis, were cultured on non-nutrient agar examined by microscope for the presence of free-living protozoa. All protozoa were identified by 18S rRNA gene sequencing. RESULTS:A total of 20 of 233 (8.6 %) contact lens solution specimens collected from 16 patients were cultured. Acanthamoeba amoeba in 16 solutions (80 %) collected from 12 patients and Colpoda steini, Cercozoa sp., Protostelium sp. and a eukaryotic more closely related to Vermamoeba sp., were each isolated in one solution. Cercozoa sp., Colpoda sp., Protostelium sp. and Vermamoeba sp. are reported for the first time as contaminating contact lens solutions. CONCLUSION:The repertoire of protozoa in contact lens solutions is larger than previously known.
journal_name
BMC Ophthalmoljournal_title
BMC ophthalmologyauthors
Bouchoucha I,Aziz A,Hoffart L,Drancourt Mdoi
10.1186/s12886-016-0370-6subject
Has Abstractpub_date
2016-10-29 00:00:00pages
191issue
1issn
1471-2415pii
10.1186/s12886-016-0370-6journal_volume
16pub_type
杂志文章abstract:BACKGROUND:Since the normative value of the retinal macular thickness is undocumented in the Middle East, the aim of this work is to assess the normative values of the macular thickness in healthy eyes in a Middle Eastern population and its relationship with age, sex, and laterality. METHODS:One hundred sixteen indivi...
journal_title:BMC ophthalmology
pub_type: 杂志文章
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journal_title:BMC ophthalmology
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更新日期:2010-03-19 00:00:00
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更新日期:2005-04-05 00:00:00
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更新日期:2017-08-03 00:00:00
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更新日期:2019-09-05 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,多中心研究,随机对照试验
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