Temporal alterations in mRNA levels for proteinases and inhibitors and their potential regulators in the healing medial collateral ligament.

abstract：:Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PT...

journal_title：Biochemical and biophysical research communications

authors： Lee MS,Kwon EH,Choi HS,Kwon SH,Lee CH,Shim IS,Lee SK,Her S

更新日期：2010-04-02 00:00:00

abstract：:We have recently reported a novel finding that a candidate tumor suppressor gene prox1 suffered adenosine-to-inosine (A-to-I) RNA mutation without genomic mutation in a subset of human cancer cells and lost its function. Hence, screening of mutations in both cDNA and genomic DNA could be important in the analysis of c...

journal_title：Biochemical and biophysical research communications

authors： Yoshimoto T,Takahashi M,Nagayama S,Watanabe G,Shimada Y,Sakasi Y,Kubo H

更新日期：2007-07-27 00:00:00

abstract：:A better understanding of cell biology and cell-cell interactions requires three-dimensional (3-D) culture systems that more closely represent the natural structure and function of tissues in vivo. Here, we present a novel device that provides an environment for routine 3-D cell growth in vitro. We have developed a th...

journal_title：Biochemical and biophysical research communications

authors： Bokhari M,Carnachan RJ,Cameron NR,Przyborski SA

更新日期：2007-03-23 00:00:00

abstract：:The previous study showed involvement of spinal glia in tetanically sciatic stimulation-induced long-term potentiation (LTP) of C-fiber-evoked field potentials in the spinal dorsal horn. In the present study, the electrophysiological recording and paw withdrawal threshold (PWT) to von Frey stimulation were assessed fo...

journal_title：Biochemical and biophysical research communications

authors： Ying B,Lü N,Zhang YQ,Zhao ZQ

更新日期：2006-02-24 00:00:00

abstract：:A protein designated mungin, isolated from mung bean (Phaseolus mungo) seeds, possessed activity against the fungi Rhizoctonia solani, Coprinus comatus, Mycosphaerella arachidicola, Botrytis cinerea, and Fusarium oxysporum. The 18-kDa protein also possessed a novel N-terminal sequence with similarity to cyclophilins. ...

journal_title：Biochemical and biophysical research communications

authors： Ye XY,Ng TB

更新日期：2000-07-14 00:00:00

abstract：:Endoplasmic reticulum (ER) stress has been shown to play a key role during the initiation and clinical progression of the cardiovascular diseases, such as atherosclerosis. We have recently shown that expression of tissue factor pathway inhibitor (TFPI) in human monocyte-derived macrophages (MDMs) was induced by choles...

journal_title：Biochemical and biophysical research communications

authors： Espada S,Stavik B,Holm S,Sagen EL,Bjerkeli V,Skjelland M,Dahl TB,Espevik T,Kanse S,Sandset PM,Skretting G,Halvorsen B

更新日期：2017-09-16 00:00:00

abstract：:Protein phosphatase was partially purified from myofibrils of bovine heart by sequential column chromatographies. The purified protein phosphatase was immunologically identified as a delta isoform of PP1 (PP1delta). The myosin-binding subunit (MBS) of myosin-binding phosphatase (MBP) in smooth muscle was co-purified w...

journal_title：Biochemical and biophysical research communications

authors： Nishio H,Ichikawa K,Hartshorne DJ

更新日期：1997-07-30 00:00:00

abstract：:Oculopharyngeal muscular dystrophy (OPMD) is an adult-onset dominant genetic disease caused by the expansion of a GCG trinucleotide repeat that encodes the polyalanine tract at the N-terminus of the nuclear poly(A)-binding protein (PABPN1). Presence of intranuclear inclusions (INIs) containing PABPN1 aggregates in the...

journal_title：Biochemical and biophysical research communications

authors： Wang Q,Bag J

更新日期：2006-02-17 00:00:00

abstract：:Various elicitors were examined by Northern blot analysis to investigate the simultaneous induction of gene expression of antibacterial proteins such as cecropin B, attacin and lebocin from the silkworm, Bombyx mori. Lipopolysaccharide (LPS), lipid A, 2-keto-3-deoxyoctonate (KDO) and peptidoglycan (PG) triggered effic...

journal_title：Biochemical and biophysical research communications

authors： Taniai K,Furukawa S,Shono T,Yamakawa M

更新日期：1996-09-24 00:00:00

abstract：:The COP9 signalosome (CSN) is a regulator of the ubiquitin (Ub) proteasome system (UPS). In the UPS, proteins are Ub-labeled for degradation by Ub ligases conferring substrate specificity. The CSN controls a large family of Ub ligases called cullin-RING ligases (CRLs), which ubiquitinate cell cycle regulators, transcr...

journal_title：Biochemical and biophysical research communications

authors： Rockel B,Schmaler T,Huang X,Dubiel W

更新日期：2014-07-25 00:00:00

abstract：:We have characterized a novel surface protein from urea extract of whole cells of group A Streptococcus pyogenes (GAS). A major protein band (35kD) was found to hybridize with human IgG by Western blotting. A search of the N-terminal amino acid sequence of this protein by using the GAS genome sequence database reveale...

journal_title：Biochemical and biophysical research communications

authors： Kawabata S,Tamura Y,Murakami J,Terao Y,Nakagawa I,Hamada S

更新日期：2002-09-06 00:00:00

abstract：:Efficient phagocytic clearance of apoptotic spermatogenic cells and residual bodies (RBs) by Sertoli cells (SCs) is crucial for functional mature spermatogenesis. However, little is known about the molecular mechanisms underlying this SCs function. Herein, we reported for the first time that SCs-expressing metastasis ...

journal_title：Biochemical and biophysical research communications

authors： Zhu CJ,Zhang S,Liang Y,Li W

更新日期：2014-03-14 00:00:00

abstract：:Identification of functionally important sites (FIS) in proteins is a critical problem and can have profound importance where protein structural information is limited. Machine learning techniques have been very useful in successful classification of many important biological problems. In this paper, we adopt the spar...

journal_title：Biochemical and biophysical research communications

authors： Tang K,Pugalenthi G,Suganthan PN,Lanczycki CJ,Chakrabarti S

更新日期：2009-06-26 00:00:00

abstract：:The need to develop anti-influenza drugs with novel antiviral mechanisms is urgent because of the rapid rate of antigenic mutation and the emergence of drug-resistant viruses. We identified a novel anti-influenza molecule by screening 861 plant-derived natural components using a high-throughput image-based assay that ...

journal_title：Biochemical and biophysical research communications

authors： Chang SY,Park JH,Kim YH,Kang JS,Min JY

更新日期：2016-03-04 00:00:00

abstract：:Peripheral blood mononuclear cells (PBMCs) from normal adults secreted small amounts of human growth hormone (GH; 0.2-0.6 pg/10(5) cells/7 days culture) as measured by a highly sensitive enzyme immunoassay. Stimulation of PBMCs with phytohemagglutinin (PHA) consistently showed a 4-6 fold increase in GH secretion. Tran...

journal_title：Biochemical and biophysical research communications

authors： Hattori N,Shimatsu A,Sugita M,Kumagai S,Imura H

更新日期：1990-04-30 00:00:00

abstract：:The cyclin-dependent kinase (CDK) inhibitor p18 blocks progression of the cell cycle by associating with the cyclin D-dependent kinases CDK6 and CDK4. To better understand the regulation of p18 gene expression, we isolated full-length cDNA clones from a human BT-20 breast cancer cell cDNA library. These clones were th...

journal_title：Biochemical and biophysical research communications

authors： Blais A,Labrie Y,Pouliot F,Lachance Y,Labrie C

更新日期：1998-06-09 00:00:00

abstract：:Atherosclerosis-related vascular complications in beta-thalassemia/hemoglobin E (beta-thal/Hb E) patients may result from iron induced oxidation of lipoproteins. To identify the specific site of oxidative damage, changes in lipid fluidity at different regions in LDL and HDL particle were investigated using two fluores...

journal_title：Biochemical and biophysical research communications

authors： Morales NP,Charlermchoung C,Luechapudiporn R,Yamanont P,Fucharoen S,Chantharaksri U

更新日期：2006-11-24 00:00:00

abstract：:The cooperation of phosphofructokinase-2 and fructose-2,6-bisphosphatase is investigated. Experimentally derived rate laws of the kinase and bisphosphatase activities introduced into the respective differential equations permitted to describe the time evolution of fructose-2,6-bisphosphate to quasi-stationary levels. ...

journal_title：Biochemical and biophysical research communications

authors： Kretschmer M,Schellenberger W,Hofmann E

更新日期：1985-09-16 00:00:00

abstract：:The binding site(s) in rabbit liver fructose-1,6-bisphosphatase for the active site binding ligand, fructose 6-phosphate, and the inhibitor, fructose 2,6-bisphosphate, have been investigated by using nuclear magnetic resonance spectroscopy. The distance from a nitroxide spin label to the bound ligands and the distance...

journal_title：Biochemical and biophysical research communications

authors： Liu F,Roy M,Fromm HJ

更新日期：1989-06-15 00:00:00

abstract：:In vitro metabolism of the herbicide bentazon was studied in microsomal membranes isolated from 6-day-old etiolated corn shoots. Microsomes isolated from shoots of nontreated seeds did not metabolize bentazon when assayed with NADPH or peroxides. However, microsomes isolated from shoots of seeds pretreated with naphth...

journal_title：Biochemical and biophysical research communications

authors： McFadden JJ,Gronwald JW,Eberlein CV

更新日期：1990-04-16 00:00:00

abstract：:A novel procedure is described for the assignment of the position of O-acylated residues in the sequence of oligosaccharides. The procedure is both rapid and sensitive and complements existing carbohydrate techniques. The procedure involves the use of derivatisation, using conditions under which naturally occurring O-...

journal_title：Biochemical and biophysical research communications

authors： Dell A,Tiller PR

更新日期：1986-03-28 00:00:00

abstract：:The only outer mitochondrial membrane cytochrome b(5) examined to date, from rat (rOM b(5)), exhibits greater stability than known mammalian microsomal (Mc) isoforms, as well as a much higher kinetic barrier for hemin dissociation and a more negative reduction potential. A BlastP search of available databases using th...

journal_title：Biochemical and biophysical research communications

authors： Altuve A,Wang L,Benson DR,Rivera M

更新日期：2004-02-06 00:00:00

abstract：:To investigate the functions of key domains of the epidermal growth factor receptor (EGFR), various EGFR-derived peptide sequences were expressed in Escherichia coli as glutathione S-transferase (GST) fusion proteins. The purified fusion proteins (GST-TK0-8) were tested as substrates for the tyrosine kinase activities...

journal_title：Biochemical and biophysical research communications

authors： Koland JG,O'Brien KM,Cerione RA

更新日期：1990-01-15 00:00:00

abstract：:Diaminopimelate (DAP) epimerase catalyzes the stereoinversion of ll-DAP to meso-DAP, a precursor of l-lysine and an essential component of the bacterial peptidoglycan. This function is vital to bacteria and the enzyme therefore represents an attractive target for the design of novel anti-bacterials. DAP epimerase belo...

journal_title：Biochemical and biophysical research communications

authors： Pillai B,Cherney M,Diaper CM,Sutherland A,Blanchard JS,Vederas JC,James MN

更新日期：2007-11-23 00:00:00

abstract：:Mouse embryos at implantation stage were stained successfully with lacto-N-fucopentaose I (LNF-I) bearing neoglycoprotein labeled with rhodamine synthesized by us for the first time. The fluorescent neoglycoproteins carrying LNF-II, -III, LND-I, or LNT failed to stain the embryos. The embryo was stained only at the ce...

journal_title：Biochemical and biophysical research communications

authors： Yamagata T,Yamazaki K

更新日期：1991-12-31 00:00:00

abstract：:Methylation-specific PCR (MSP) is frequently used to distinguish methylated alleles in the genome. Sequences that have been incompletely converted during bisulfite treatment are frequently co-amplified during MSP. For accurate MSP, it is important to detect methylated sequences in a background of unconverted DNA with ...

journal_title：Biochemical and biophysical research communications

authors： Sasaki M,Anast J,Bassett W,Kawakami T,Sakuragi N,Dahiya R

更新日期：2003-09-19 00:00:00

abstract：:Immunoblot analysis of the conditioned medium of v-Ha-ras-transformed NIH3T3 cells showed that Ras protein is secreted in the culture fluid. Because cathepsins L and B whose activities are inhibited by Ras are also secreted, possible association between Ras and cathepsins was investigated by immunoprecipitation and fo...

journal_title：Biochemical and biophysical research communications

authors： Hiwasa T,Kominami E

更新日期：1995-11-22 00:00:00

abstract：:L-amino acids represent the most common amino acid form, most notably as protein residues, whereas D-amino acids, despite their rare occurrence, play significant roles in many biological processes. Amino acid racemases are enzymes that catalyze the interconversion of L- and/or D-amino acids. McyF is a pyridoxal 5'-pho...

journal_title：Biochemical and biophysical research communications

authors： Cao DD,Zhang CP,Zhou K,Jiang YL,Tan XF,Xie J,Ren YM,Chen Y,Zhou CZ,Hou WT

更新日期：2019-07-05 00:00:00

abstract：AIM/BACKGROUND:CD99 participate in neutrophil infiltration after inflammatory events; however, despite the important role of inflammation in ischemic stroke, the role of CD99 in ischemic stroke remains unclear. METHOD:In the present study, we detected the protein expression of CD99, ICAM-1, and CD31 (PECAM-1) in oxyge...

journal_title：Biochemical and biophysical research communications

authors： Hu T,Sun R,Huang F,Liu X,Duan Z,Ye R,Li Y,Xiao L,Guo Z,Liu Q,Zhu W

更新日期：2020-06-04 00:00:00

abstract：:To repair bone defects, we evaluate the in-vitro and in-vivo osteogenic activities of a novel tissue-engineered bone (TEB) by elaborately combining biomimetic calcium phosphate (BioCaP) granules with internally-incorporated simvastatin (SIM) and human adipose-derived stem cells (hASCs). First, we constructed BioCaP wi...

journal_title：Biochemical and biophysical research communications

authors： Zhang X,Jiang W,Liu Y,Zhang P,Wang L,Li W,Wu G,Ge Y,Zhou Y

更新日期：2018-01-01 00:00:00