Abstract:
:Telomere proteins protect the chromosomal terminus from nucleolytic degradation and end-to-end fusion, and they may contribute to telomere length control and the regulation of telomerase. The current studies investigate the effect of Oxytricha single-stranded telomere DNA-binding protein subunits alpha and beta on telomerase elongation of telomeric DNA. A native agarose gel system was used to evaluate telomere DNA-binding protein complex composition, and the ability of telomerase to use these complexes as substrates was characterized. Efficient elongation occurred in the presence of the alpha subunit. Moreover, the alpha-DNA cross-linked complex was a substrate for telomerase. At higher alpha concentrations, two alpha subunits bound to the 16-nucleotide single-stranded DNA substrate and rendered it inaccessible to telomerase. The formation of this alpha . DNA . alpha complex may contribute to regulation of telomere length. The alpha . beta . DNA ternary complex was not a substrate for telomerase. Even when telomerase was prebound to telomeric DNA, the addition of alpha and beta inhibited elongation, suggesting that these telomere protein subunits have a greater affinity for the DNA and are able to displace telomerase. In addition, the ternary complex was not a substrate for terminal deoxynucleotidyltransferase. We conclude that the telomere protein inhibits telomerase by rendering the telomeric DNA inaccessible, thereby helping to maintain telomere length.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Froelich-Ammon SJ,Dickinson BA,Bevilacqua JM,Schultz SC,Cech TRdoi
10.1101/gad.12.10.1504subject
Has Abstractpub_date
1998-05-15 00:00:00pages
1504-14issue
10eissn
0890-9369issn
1549-5477journal_volume
12pub_type
杂志文章abstract::Eukaryotic translation initiation begins with ribosomal recruitment of aminoacylated initiator tRNA (Met-tRNA(Met)(i)) by eukaryotic initiation factor eIF2. In cooperation with eIF3, eIF1, and eIF1A, Met-tRNA(Met)(i)/eIF2/GTP binds to 40S subunits yielding 43S preinitiation complexes that attach to the 5'-terminal reg...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1957510
更新日期:2010-08-15 00:00:00
abstract::Mice mutant for the bHLH gene Hes1, which is known to keep cells in a proliferative state, mostly lack thymus. Transfer of Hes1-null fetal liver cells into RAG2-null host mice normally reconstitutes B cells but fails to generate mature T cells in the thymus. In the reconstituted thymus, T cell differentiation is arres...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.9.1203
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1265905
更新日期:2005-01-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2010-06-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.15.2028
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1286005
更新日期:2005-04-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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更新日期:2000-01-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.17.2193
更新日期:1995-09-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.417607
更新日期:2007-03-15 00:00:00
abstract::Transcription of the cell-cycle-regulated human histone genes increases approximately fivefold during S phase. One step toward the elucidation of the biochemical mechanisms that govern cell-cycle-regulated expression of these genes is to purify and characterize the transcription factors that regulate these promoters. ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.12b.1700
更新日期:1988-12-01 00:00:00
abstract::Small noncoding RNAs, including small interfering RNAs (siRNAs) and micro RNAs (miRNAs) of approximately 21 nucleotides (nt) in length, have emerged as potent regulators of gene expression at both transcriptional and post-transcriptional levels in diverse organisms. Here we report the identification of a novel class o...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1434406
更新日期:2006-07-01 00:00:00
abstract::Gene silencing by double-stranded RNA is a widespread phenomenon called RNAi, involving homology-dependent degradation of mRNAs. Here we show that RNAi is established in the Drosophila female germ line. mRNA transcripts are translationally quiescent at the arrested oocyte stage and are insensitive to RNAi. Upon oocyte...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.990802
更新日期:2002-08-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.267252.115
更新日期:2015-08-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.21.2828
更新日期:1999-11-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.6.770
更新日期:1989-06-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.243584.114
更新日期:2014-10-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-02-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.23.2936
更新日期:1995-12-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.9.1087
更新日期:1995-05-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2015-11-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.277533.116
更新日期:2016-05-01 00:00:00
abstract::Mutations that influence lin-12 activity in Caenorhabditis elegans may identify conserved factors that regulate the activity of lin-12/Notch proteins. We describe genetic evidence indicating that sel-10 is a negative regulator of lin-12/Notch-mediated signaling in C. elegans. Sequence analysis shows that SEL-10 is a m...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.23.3182
更新日期:1997-12-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.892101
更新日期:2001-07-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.15.1930
更新日期:1996-08-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1999-12-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.292318.116
更新日期:2017-03-15 00:00:00