Abstract:
:On different tissue sections, binding of gliadin to reticular matrix components was observed which was Ca2+-dependent, inhibited by Cu2+ and Zn2+ ions, by putrescine, and by preincubation with antibodies against tissue transglutaminase (tTG) suggesting that binding of gliadin is mediated by tTG. tTG was able to bind to gliadin and fibronectin fixed to microplates. Furthermore, tTG mediated binding of gliadin to fibronectin coated to microplates. On tissue sections, treatment with sera containing coeliac disease autoantibodies yielded staining patterns very similar to that of bound gliadin. Dual label experiments by means of conventional and laser scanning microscopy revealed that most of autoantibody binding sites are matched by bound gliadin. However, lack of competition between gliadin and autoantibody binding hints to ligands in very close vicinity of this enzyme. Furthermore, there were several autoantibody binding regions which did not bind gliadin. This implies the existence of further autoantigenic epitopes not related to tTG.
journal_name
Autoimmunityjournal_title
Autoimmunityauthors
Uhlig H,Osman AA,Tanev ID,Viehweg J,Mothes Tdoi
10.3109/08916939808995366subject
Has Abstractpub_date
1998-01-01 00:00:00pages
185-95issue
4eissn
0891-6934issn
1607-842Xjournal_volume
28pub_type
杂志文章相关文献
AUTOIMMUNITY文献大全abstract::Epitopes for > 95% stimulating thyrotropin receptor autoantibodies (TSHRAbs) causally implicated in Graves' disease (Basedow's disease or primary hyperthyroidism) have been identified on on the N-terminal portion of the TSHR extracellular domain, residues 8-165. If the stimulating TSHRAb activity is solely dependent o...
journal_title:Autoimmunity
pub_type: 杂志文章,评审
doi:10.1080/08916930310001604199
更新日期:2003-09-01 00:00:00
abstract::Although costimulatory molecules have been shown to play crucial roles in the immune response, their involvement in the pathogenesis of Sjögren's syndrome is incompletely understood. In this study, we evaluated the relationship between the severity of spontaneous Sjögren's syndrome-like autoimmune sialadenitis in MRL/...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916934.2012.757307
更新日期:2013-06-01 00:00:00
abstract::Systemic sclerosis (SSc) is characterized by extensive fibrosis, vasculopathy and activation of the immune system. Fibrosis can be caused by profibrotic cytokines, such as transforming growth factor-beta (TGFbeta), interleukin-4 (IL-4), platelet-derived growth factor (PDGF), and connective tissue growth factor. Vascul...
journal_title:Autoimmunity
pub_type: 杂志文章,评审
doi:10.1080/16066350500095415
更新日期:2005-03-01 00:00:00
abstract::IL-6 is a paracrine and autocrine cytokine, which acts in the regulation of immunological and inflammatory processes. Its production can be observed in different cell types, as well as thyrocytes. The purpose of the study was to examine the serum IL-6 levels between the patients with Graves' disease (N = 47) and witho...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939708996275
更新日期:1997-01-01 00:00:00
abstract::Recent years have witnessed an explosive growth in available biological data pertaining to autoimmunity research. This includes a tremendous quantity of sequence data (biological structures, genetic and physical maps, pathways, etc.) generated by genome and proteome projects plus extensive clinical and epidemiological...
journal_title:Autoimmunity
pub_type: 杂志文章,评审
doi:10.1080/08916930601062437
更新日期:2006-12-01 00:00:00
abstract::Insulin-dependent diabetes mellitus (IDDM) is preceded by the presence of antibodies against islet proteins including a protein tyrosine phosphatase (PTP) designated IA-2. Recently, we cloned a novel PTP named IAR which shares 43% sequence identity with IA-2 and is recognised by antibodies from a majority of patients ...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939808993841
更新日期:1998-01-01 00:00:00
abstract::The MRL/lpr model of SLE resembles human lupus in its various immunopathologic characteristics including the presence of high-level IgG and anti-DNA antibody production and multisystem organ involvement (nephritis, arthritis, and vasculitis). Our previous studies have shown that IL-1 overactivity in B cells plays a po...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939609001949
更新日期:1996-01-01 00:00:00
abstract::Granulocyte macrophage-colony stimulating factor (GM-CSF) is a pleiotrophic proinflammatory cytokine that augments adaptive immunity by linking it to innate immunity. Experimental autoimmune gastritis is an animal model of human autoimmune gastritis and pernicious anaemia. We have previously shown that GM-CSF is expre...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.1080/08916930290005954
更新日期:2002-02-01 00:00:00
abstract::Autoantigen-reactive T lymphocytes have been implicated in the pathogenesis organ-specific autoimmune disease. Thyroglobulin (Tg) is one of the primary autoantigens associated with autoimmune lymphocytic thyroiditis (LT). These experiments investigated the pathogenicity of a lymphocyte line derived from spontaneously-...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939608995327
更新日期:1996-01-01 00:00:00
abstract::The thymic medulla is a complex microenvironment which plays a crucial role in central tolerance induction. Using a quantitative histological analysis of non-obese diabetic (NOD) mice, we show that the medulla undergoes several structural modifications during the course of the disease in NOD mice. Indeed, the majority...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939908994070
更新日期:1999-01-01 00:00:00
abstract::Myocarditis, often initiated by viral infection, may progress to autoimmune inflammatory heart disease, dilated cardiomyopathy and heart failure. Although cardiac myosin is a dominant autoantigen in animal models of myocarditis and is released from the heart during viral myocarditis, the characterization, role and sig...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.1080/08916930802031579
更新日期:2008-09-01 00:00:00
abstract::Anaemia is commonly observed in chronic inflammatory conditions, including systemic lupus erythematosus (SLE), where ∼50% of patients display clinical signs of anaemia. Mutation at the aspartate residue 18 of the three prime repair exonuclease 1 (TREX1) gene causes a monogenic form of cutaneous lupus in humans and the...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.1080/08916934.2018.1522305
更新日期:2018-11-01 00:00:00
abstract::The cytokine profile and occurrence of apoptosis during experimental melanin-protein induced uveitis (EMIU) were investigated and compared with that of experimental autoimmune uveoretinitis (EAU). EMIU or EAU was induced in Lewis rats. Eyes were collected at different time points after immunization. Cytokine mRNA expr...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939908993851
更新日期:1999-01-01 00:00:00
abstract::The goal of contemporary research in pemphigus vulgaris and pemphigus foliaceus is to achieve and maintain clinical remission without corticosteroids. Recent advances of knowledge on pemphigus autoimmunity scrutinize old dogmas, resolve controversies, and open novel perspectives for treatment. Elucidation of intimate ...
journal_title:Autoimmunity
pub_type: 杂志文章,评审
doi:10.3109/08916934.2011.606444
更新日期:2012-02-01 00:00:00
abstract::The concordance rate of Graves' disease in pairs of identical twins of 30 to 60% points to the influence of environmental factors, and infections have often been incriminated in the pathogenesis. More than 15 years ago we demonstrated an increased frequency of antibodies to Yersinia enterocolitica (Y. ent.) serotype 3...
journal_title:Autoimmunity
pub_type: 杂志文章,评审
doi:10.3109/08916939009087588
更新日期:1990-01-01 00:00:00
abstract::The objective of this study was to identify new autoantibodies that could be useful for the diagnosis of rheumatoid arthritis (RA) using immunoblotting on synovial membrane proteins which represent the best source of candidate RA autoantigens. A new target protein with a molecular weight of 26 kDa was found to be reco...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.1080/08916930701417473
更新日期:2007-07-01 00:00:00
abstract::Guillain-Barre syndrome (GBS) is an autoimmune disorder of the peripheral nervous system. However, GBS patients frequently display a psychosyndrome, indicating an accompanying involvement of the central nervous system (CNS), although the cause is not understood. Hepatic encephalopathy is possibly the best characterize...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.1080/08916930412331279840
更新日期:2004-09-01 00:00:00
abstract::The biological basis for autoimmunity and immunoincompetence in the BB rat has yet to be localized. In spite of normal thymic histology, thymocyte subsets and blastogenesis, thymus gland products (thymosins) have yet to be studied. In the present report, thymus gland function was studied by measuring thymosin alpha 1 ...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916938809001924
更新日期:1988-01-01 00:00:00
abstract::Carbonic anhydrase II (CAII) is expressed on alveolar epithelium and participates to CO2 elimination, fluid secretion and post-capillary pH regulation. CAII is overexpressed in animal models of lung fibrosis in sites of epithelial injury. Autoantibodies directed against CAII (anti-CAII) have been described in sera fro...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.1080/0891693031000079239
更新日期:2003-03-01 00:00:00
abstract::Infections with different helminth species have been observed to ameliorate a variety of chronic inflammatory diseases. Herein, we show that the natural murine helminth species, Heligmosomoides polygyrus bakeri (Hp) is capable of attenuating disease severity in two different inflammatory arthritis models. Furthermore,...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.1080/08916934.2016.1261837
更新日期:2017-05-01 00:00:00
abstract::Under-nutrition impairs immune responses, but far less is known about the impact of over-nutrition, such as obesity, on the response to vaccines. We measured the effect of childhood overweight status on inflammatory mediators, circulating immunoglobulins and tetanus antibodies in fifteen overweight children (BMI > 85 ...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.1080/08916930600597326
更新日期:2006-03-01 00:00:00
abstract::Subcutaneous insulin treatment of young diabetes prone BB rats has been shown previously to suppress the development of autoimmune diabetes. In this study the hypothesis was tested that exogenous insulin may deviate the autoimmune process by acting on the Th1/Th2 cytokine balance in the pancreas. BB rats were implante...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939709009544
更新日期:1997-01-01 00:00:00
abstract::Myelin oligodendrocyte glycoprotein (MOG) is postulated to be a target autoantigen in multiple sclerosis (MS). Here we investigated the encephalitogenicity of an immunodominant epitope of MOG, peptide 35-55, in various strains of mice. An MS-like disease was induced in NOD/Lt mice (H-2g7) and C57BL/6 mice (H-2b) by a ...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939809003872
更新日期:1998-01-01 00:00:00
abstract::Only one out of 57 A-/A- rabbits immunized with rat or guinea-pig myelin developed clinical signs suggestive of EAE. On the contrary, clinical signs of acute or chronic EAE were found in two thirds of the 102 A+/A+ and A+/A- rabbits immunized in the same way. About one third of the diseased animals had reversible acut...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939008993369
更新日期:1990-01-01 00:00:00
abstract::We reported a patient who gave birth to 3 children with transient neonatal hypothyroidism. She had 3 different antibodies (Ab) to the thyrotropin receptor (TSHR) in her serum, viz., TSH binding-inhibiting (TBIAb), thyroid-stimulating (TSAb) and an additional stimulating Ab (SAb). The SAb differed from TSAb in that its...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939409008006
更新日期:1994-01-01 00:00:00
abstract::Non obese diabetic (NOD) mice spontaneously develop thyroiditis in addition to diabetes. Mononuclear cells begin to infiltrate the thyroid of these animals in the first month of life. The expression of major histocompatibility complex (MHC) class II (Ia) antigens by cells in the thyroid from NOD mice of various ages w...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939409014653
更新日期:1994-01-01 00:00:00
abstract::We have used the MRL/lpr murine model of spontaneous lupus to investigate three questions in infection immunity: (1) does mycobacterial infection have any effect on the mortality of the autoimmunity-prone lpr mice?; (2) does the infection modify the progression of kidney disease of lpr lupus?; and (3) does the lpr gen...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939308993323
更新日期:1993-01-01 00:00:00
abstract:OBJECTIVE:To assess muscle strength in patients with idiopathic inflammatory myopathies (IIM) using neuromuscular scales and isokinetic testing. METHODS:Muscle function was evaluated in 27 IIM patients being followed at the Rheumatology Unit of the University of Pisa using: (i) a modified version of the grading system...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.1080/08916930600623817
更新日期:2006-05-01 00:00:00
abstract::Chronic immune thrombocytopenia (ITP) is characterized by autoimmune-mediated platelet destruction and impairment of thrombopoiesis. Mesenchymal stem cells (MSCs) are proposed to exhibit immune modulatory functions in self-tolerance maintenance. In this study, we aimed to characterize phenotypically and functionally b...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916934.2014.938320
更新日期:2014-12-01 00:00:00
abstract::Thyroid lymphocyte RNA from a Hashimoto patient exhibiting high titre serum IgG autoantibodies against thyroglobulin (Tg) has been used to construct a Fab library in phage lambda. Screening of this library with radioiodinated Tg has permitted the cloning of an anti-Tg antibody (MH52) with an affinity of 4.5 x 10(9) mo...
journal_title:Autoimmunity
pub_type: 杂志文章
doi:10.3109/08916939209150320
更新日期:1992-01-01 00:00:00