Abstract：
:Heparin and other antithrombotic drugs besides their anticoagulant action could have a profibrinolytic effect. We have analyzed the effect of unfractionated heparin (UFH) and hirudin on PAI-1 gene expression in human umbilical vein endothelial cells (HUVEC). Cells were stimulated with UFH (1 and 10 IU/ml) and hirudin (20 and 100 TIU/ml). Samples were obtained before and 2, 6, and 24 hours after stimulation. mRNA analysis was conducted by reverse transcription followed by polymerase chain reaction, and PAI-1 antigen was determined by ELISA. Addition of UFH (10 IU/ml) to HUVEC resulted in a decrease of PAI-1 mRNA at 6 hours (40% reduction) and 24 hours (60% reduction) and PAI-1 antigen. Hirudin, however, did not modify significantly the PAI-1 mRNA nor the inhibitor secretion. The addition of UFH (10 or 100 IU/ml) to endotoxin-stimulated HUVEC also reduced the increased PAI-1 mRNA and antigen secretion (45%), whereas no effect could be observed with hirudin. Our results suggest that UFH, but not hirudin, by reducing the endothelial expression of PAI-1 might have a profibrinolytic effect.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Orbe J,Montes R,Zabalegui N,Pérez-Ruiz A,Páramo JAdoi
10.1016/s0049-3848(98)00203-5subject
Has Abstractpub_date
1999-05-01 00:00:00pages
137-45issue
3eissn
0049-3848issn
1879-2472pii
S0049-3848(98)00203-5journal_volume
94pub_type
杂志文章abstract：INTRODUCTION:Pre-eclampsia is an important cause of maternal morbidity and mortality. Its etiology is still unknown. Clinical symptoms correlate with activation of coagulation and inherited thrombophilia has been associated with pre-eclampsia. ABO blood group has been associated with thrombotic disorders and pre-eclamp...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2008.11.012
更新日期：2009-06-01 00:00:00
abstract：:Thromboangiitis obliterans (TAO), or Buerger's disease, is a segmental occlusive inflammatory disorder of the arteries and veins, and etiopathogenesis is still obscure. In the present study we investigated the prevalence of prothrombin 20210 G-->A, factor V 1691 G-->A (Factor V Leiden), and factor V 4070 A-->G (His 12...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00310-8
更新日期：2000-11-01 00:00:00
abstract：:Whilst the term cancer associated thrombosis (CAT) offers an overarching term for all thrombotic events encountered during the cancer journey, the reality is that this is a far too simplistic reflection of a complex multifactorial process occurring within a heterogeneous population. The management of CAT needs to cons...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/S0049-3848(16)30115-3
更新日期：2016-04-01 00:00:00
abstract：INTRODUCTION:In contrast to unfractionated heparin (UFH), use of low-molecular weight heparin (LMWH) during pregnancy has not been reported to be associated with a significant decrease in bone mineral density (BMD). The aim of this study was to investigate whether long-term use of LMWH during pregnancy is associated wi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.05.016
更新日期：2016-07-01 00:00:00
abstract：:The effect of the vasopressin analogue DDAVP on Factor VIII in plasma has been extensively studied in humans. To examine the effect of new and potentially better analogues a suitable animal model has to be established. In the present study trained Beagle dogs were injected with DDAVP and the time course of the Factor ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90363-x
更新日期：1987-09-01 00:00:00
abstract：:Thrombolysis is an effective but potential deleterious therapy and should therefore be limited to patients with acute intracerebral vessel occlusion and salvageable tissue. MRI currently develops towards the new diagnostic standard for the selection of stroke patients eligible for acute thrombolytic treatment and acut...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/s0049-3848(01)00309-7
更新日期：2001-09-30 00:00:00
abstract：:The changes in the fibrinolytic system of rats induced by repeated injections of tissue plasminogen activator (TPA) have been studied. The euglobulin fraction fibrinolytic activity increases 10 min after the first injection of TPA. Repeated injections of TPA for one or more days do not stimulate the fibrinolytic activ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90075-5
更新日期：1982-08-01 00:00:00
abstract：:Atherothrombosis is the leading cause of morbidity and mortality in patients with diabetes mellitus. Several mechanisms contribute to the diabetic prothrombotic state, including endothelial dysfunction, coagulative activation and platelet hyper-reactivity. In particular, diabetic platelets are characterised by dysregu...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2011.11.052
更新日期：2012-03-01 00:00:00
abstract：:Rabbit platelets pretreated with CV-3988 had reduced responsiveness to platelet activating factor(PAF) even after the platelets had been washed with a buffer. This was due to its tight binding to receptor sites for PAF, but not conformational changes of the receptor, because the platelets that were pretreated with CV-...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90215-5
更新日期：1987-04-01 00:00:00
abstract：:Diabetes is associated with multiple disorders including metabolic, cellular and blood disturbances leading to vascular complications. Increased circulating levels of platelet-leukocyte aggregates (PLA) have been described in several thrombotic diseases. In this study, we have evaluated circulating PLA in diabetic pat...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.07.016
更新日期：2008-01-01 00:00:00
abstract：:The variant von Willebrand disease (vWd) variant type 2M (Vicenza) was identified in 13 patients of 7 unrelated families. 11 patients were from different parts of germany and 2 patients from Turkey. Hitherto this variant of vWd has been described only in two families originating from the province of Vicenza in Norther...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00104-7
更新日期：1997-07-01 00:00:00
abstract：:Cooperative effects of PAF-acether and arachidonic acid in blood platelet activation were studied in human platelet-rich plasma. Using a combination of low concentrations of PAF-acether and subthreshold amounts of exogenous arachidonic acid an enhancement of aggregation and an increased formation of malondialdehyde ar...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90080-x
更新日期：1984-02-15 00:00:00
abstract：INTRODUCTION:The contact system proteins factor XII (FXII), prekallikrein (PK) and high molecular weight kininogen (HK) have roles in coagulation, fibrinolysis, thrombin-induced platelet activation, cell adhesion and angeogenisis. It has been suggested that inherited or acquired deficiencies of these proteins may be ri...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/j.thromres.2004.05.005
更新日期：2004-01-01 00:00:00
abstract：:A prolongation of the thrombin clotting time by at least 25% was found in the presence of 8 of 26 IgG myeloma proteins, 2 of 5 IgA proteins and 1 of 10 macroglobulins, in a concentration of 10 mg/ml. Normal pooled immunoglobulin in concentrations up to 83 mg/ml were inactive. 2 IgG and 2 IgM monoclonal immunoglobulins...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90315-3
更新日期：1984-07-01 00:00:00
abstract：:The relationship between platelet aging and early markers of membrane activation have not been defined clearly. Activation markers expressed during prolonged storage are similar if not identical to those that appear after exposure to thrombin. Using flow cytometry, we investigated platelet membrane expression of CD62P...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00173-x
更新日期：1999-02-15 00:00:00
abstract：:This study evaluates the role of neutrophil granulocytes in mediating acute phase stimulation of fibrinogen synthesis. Turpentine was administered to neutropenic and non-neutropenic rats and fibrinogen synthetic rates measured in an isolated liver perfusion system. Using the (14C) carbonate technique for the measureme...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90032-9
更新日期：1982-10-01 00:00:00
abstract：:Fibrinogen has been reported to interact with phospholipid; however, the properties of this binding interaction have not been characterized. Purified preparations of human fibrinogen bound to small unilamellar vesicles containing phosphatidylserine (PS) as measured by light scattering and radioisotope filtration. Bind...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00058-4
更新日期：1999-09-15 00:00:00
abstract：:The three main components involved in thrombosis and haemostasis are thrombin, platelets, and plasmin. Almost all inhibitors of thrombosis are focused either on the inhibition of thrombin or on the inhibition of platelets. We designed a construct using the fibrinolytic activity of staphylokinase, fused via a cleavable...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00277-6
更新日期：1997-12-01 00:00:00
abstract：UNLABELLED:Suboptimal gastrointestinal absorption is a problem for many direct thrombin inhibitors. The studies presented herein describe the new oral direct thrombin inhibitor H 376/95, a prodrug with two protecting residues added to the direct thrombin inhibitor melagatran. Absorption properties in vitro: H 376/95 is...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00399-6
更新日期：2001-02-01 00:00:00
abstract：INTRODUCTION:Genetic and autoimmune risk factors contribute to the development of thrombotic thrombocytopenic purpura (TTP) but triggers are needed to bring about acute disease. The aim of the study was to investigate the association of neutrophil activation with acute TTP, to assess whether neutrophil activation chang...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.01.034
更新日期：2014-04-01 00:00:00
abstract：:We have developed a new bioassay for endothelium-derived relaxing factor (EDRF) or nitric oxide (NO) using human [3H]guanosine triphosphate (GTP)-labeled platelets. The labeled platelets were preincubated with isobutyl-methylxanthine and co-cultured with endothelial cells and the [3H]cyclic guanosine monophosphate (cG...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00082-3
更新日期：1995-06-15 00:00:00
abstract：:Cell based models of coagulation (CBM) have provided mechanistic insight into numerous hematological issues for nearly two decades. This review discusses another coagulation model system--the clot lifespan model (CLSM)--that has been designed to compliment the CBM-based approach to elucidating the mechanisms responsib...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2007.09.003
更新日期：2008-01-01 00:00:00
abstract：:Washed human platelets were incubated with commercial factor VIII concentrate, or with purified factor VIII coagulant moiety. Platelets were then washed again and lysed by sonication. VIII:Ag and vWf:Ag were measured in the platelet lysate prior to and after incubation of the lysate with phospholipase C (PL-C). Platel...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90223-4
更新日期：1987-02-15 00:00:00
abstract：OBJECTIVES:In this randomized management study, we examined the safety of withholding anticoagulation on the basis of negative impedance plethysmography (IPG) compared to negative contrast venography (CV) in symptomatic patients with a first episode of clinically suspected deep vein thrombosis (DVT), and we determined ...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0049-3848(01)00222-5
更新日期：2001-04-01 00:00:00
abstract：:Incubation of platelets with pronase or chymotrypsin results in the exposure of fibrinogen receptors. We determined that these enzymes did not affect the membrane fluidity as evaluated by the depolarization of the fluorescence of 1,6-diphenyl-1,3,5 hexatriene (DPH). There was no significant difference in either the de...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90124-0
更新日期：1985-07-01 00:00:00
abstract：BACKGROUND:To investigate the impact of atrial fibrillation/flutter (AF) on adverse in-hospital outcomes in hospitalized surgical patients. METHODS:The nationwide German inpatient sample of the years 2005-2018 was used for this analysis. Surgical patients were stratified by AF and compared. Logistic regression models ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.10.015
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:Clauss fibrinogen assay (CFA) is widely used as a screening test to detect fibrinogen disorders. However, CFA alone cannot distinguish quantitative and qualitative defects because it depends on functional fibrinogen activity (Ac), and fibrinogen antigen (Ag) determination is required to classify fibrinogen d...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.12.018
更新日期：2019-02-01 00:00:00
abstract：:Tissue factor is an ubiquitous membrane-anchored glycoprotein that initiates blood coagulation by forming a complex with circulating factors VII and VIIa. Under normal circumstances, endothelial cells do not express tissue factor but, in some pathological situations, when the endothelium or the monocytes are exposed t...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90086-8
更新日期：1994-01-15 00:00:00
abstract：BACKGROUND:Critical care patients are prone to venous thromboembolism (VTE) and, thus, pharmacological thromboprophylaxis is generally advised. Low-molecular weight heparins (LMWHs) have become the drug of choice in ICU patients, since their predictable and reproducible dose response. Monitoring their pharmacological e...
journal_title：Thrombosis research
pub_type： 杂志文章,meta分析
doi：10.1016/j.thromres.2015.12.016
更新日期：2016-03-01 00:00:00
abstract：:Hemophilia patients with inhibitors against FVIII/FIX present with a major challenge in the clinical practice. In the 1970s such patients were treated in association with emergency situations and essential surgery using huge amounts of FVIII/FIX concentrates often preceded by extracorporal adsorption of antibodies aga...
journal_title：Thrombosis research
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.thromres.2010.01.021
更新日期：2010-04-01 00:00:00
