Abstract:
BACKGROUND:Decapeptides derived from human HLA class I sequences have been shown to prolong allograft survival. The mechanism of action of these peptides has been uncertain, because they act in an MHC unrestricted manner. Recently, it was found that these peptides bind heme oxygenase 1 (HO-1). In the present study, we sought to determine whether local delivery of these peptides through gene transfer could extend allograft survival, and to explore the underlying mechanisms. METHODS:C57BL/6 neonatal hearts were transplanted to CBA/J recipients and the peptide, or plasmid DNA encoding the peptide, was injected directly into the allograft at the time of the transplant. RESULTS:Direct injection of 1 microg of the B2702 peptide into the allograft did not prolong survival (13.3+/-0.8 vs. 13.4+/-0.8 days for untreated controls), but injection of 400 microg of peptide did extend survival (22.0+/-0.6). Injection of plasmid DNA encoding the B2702 peptide was superior to peptide delivery, extending graft survival to 30.8+/-1.5 days. Similar results were obtained using another plasmid encoding the rationally designed peptide BC1 (28.5+/-1.7), whereas no significant prolongation was observed using a plasmid encoding the control peptide B2705 (16.5+/-1.0). To explore the hypothesis that these peptides exert their immunosuppressive effect by altering HO-1 activity, animals were treated with iron protoporphyrin, an inducer of HO-1 activity, or tin protoporphyrin, an inhibitor of HO-1. Treatment with iron protoporphyrin alone extended graft survival (24.5+/-1.6) and did not alter the benefit in survival seen with BC1 gene transfer (28.0+/-0.8). In contrast, treatment with tin protoporphyrin abolished the benefit of BC1 gene transfer (17.0+/-0.6). CONCLUSIONS:These results demonstrate that plasmid mediated gene transfer is an effective means for delivering immunosuppressive peptides to extend allograft survival. The experiments suggest that these peptides may act by increasing HO-1 activity and support a role for HO-1 in immune regulation and allograft survival.
journal_name
Transplantationjournal_title
Transplantationauthors
DeBruyne LA,Magee JC,Buelow R,Bromberg JSdoi
10.1097/00007890-200001150-00021subject
Has Abstractpub_date
2000-01-15 00:00:00pages
120-8issue
1eissn
0041-1337issn
1534-6080journal_volume
69pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract::The psychosocial and psychiatric impact of donating bone marrow (BM) has received little study. This mail questionnaire study of bone marrow donors whose recipient had survived more than one year post-BMT was undertaken to document the psychosocial consequences of donation on "successful" BM donors. Demographic, mood ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198604000-00014
更新日期:1986-04-01 00:00:00
abstract::OKT3 is a murine monoclonal antibody to the CD3 antigen of human T lymphocytes. The production of human antimurine antibodies after treatment with OKT3 has been perceived as a major limitation to its extended use and reuse. Treatment of 142 patients with 168 courses of OKT3 resulted in the development of antimouse ant...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198901000-00020
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND:Auxiliary partial orthotopic liver transplantation (APOLT) has been an effective alternative in acute liver failure (ALF), but clinically several problems remain to be resolved. Thus, we attempt to establish an APOLT model for ALF using a large animal and demonstrate the validity of our model. METHODS:In ex...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000243360.64554.fa
更新日期:2006-11-27 00:00:00
abstract::Pancreatic transplantation is intended to normalize carbohydrate metabolism in insulin-dependent diabetics by restoring endogenous insulin release, and it is usually performed together with kidney transplantation in patients with end-stage renal failure. A major problem in these patients is the daily control of the gr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198304000-00016
更新日期:1983-04-01 00:00:00
abstract::Immunomodulatory properties are attributed to apoptotic cells. These properties have been used to modulate allogeneic immune responses in experimental transplantation settings. In independent studies, apoptotic cell infusion has been shown to favor hematopoietic cell engraftment, to increase heart graft survival, and ...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.tp.0000269113.59857.d6
更新日期:2007-07-15 00:00:00
abstract:BACKGROUND:Renal transplant recipients are at increased risk of developing skin cancer. It remains difficult to establish the actual influence of overimmunosuppression in the development of skin cancers. We investigated whether lymphocyte subset count may predict the risk of developing skin cancer in long-term renal tr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199805150-00022
更新日期:1998-05-15 00:00:00
abstract:BACKGROUND:Posttransplantation bone disease is a well-described phenomenon; among its etiology is immunosuppressant-induced bone disease. Mycophenolate mofetil (MMF) has emerged as a promising new immunosuppressant. Our study was designed to investigate the effect of MMF on in vivo bone mineral metabolism. METHODS:Twe...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199801270-00025
更新日期:1998-01-27 00:00:00
abstract:BACKGROUND:We have reported the protective role of heme oxygenase-1 (HO-1) in the mechanism of hypoxic preconditioning. We wish to investigate the role of HO-1 in remote preconditioning (RP) against hepatic ischemia/reperfusion (I/R) injury in rats. METHODS:The remote preconditioning was produced by four cycles of 10-...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000203555.14546.63
更新日期:2006-05-15 00:00:00
abstract:BACKGROUND:An elevated terminal creatinine is frequently used as a reason for organ refusal in pediatric kidney transplantation. There is increasing evidence that adults who receive kidneys from donors with moderate to severe acute kidney injury (AKI) have similar outcomes to recipients who receive kidneys from donors ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002827
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Urolithiasis occurs in approximately 6% of adult kidney transplant (KTx) recipients. Limited data are available on urolithiasis after pediatric KTx. We report the incidence, management of, and risk factors for stone development in children after KTx. METHODS:We reviewed the medical records of 399 children w...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000139543.56886.de
更新日期:2004-11-15 00:00:00
abstract:BACKGROUND:After transplantation, donor dendritic cells (DC) migrating to the draining lymph node of the recipient are thought to play an important role in the initiation of graft rejection. In this study, we compared the in vivo migration of DC after allogeneic skin transplantation with that after congeneic skin trans...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199903270-00008
更新日期:1999-03-27 00:00:00
abstract:BACKGROUND:The authors investigated the relationship between therapeutic blood transfusion before renal transplantation and rejection rates in cyclosporine- and tacrolimus-treated patients. METHODS:In one center, 265 consecutive recipients were studied. Protocol induction was with azathioprine, prednisolone, and cyclo...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000111756.83834.A7
更新日期:2004-02-15 00:00:00
abstract::Short-term outcomes of solid organ transplantation have improved dramatically over the past several decades; however, long-term survival has remained static over the same period, and chronic rejection remains a major cause of graft failure. The importance of donor, or "passenger," lymphocytes to the induction of toler...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0000000000002001
更新日期:2018-03-01 00:00:00
abstract::Surgical site infection (SSI) is an important cause of morbidity after orthotopic liver transplantation (OLT). Perioperative antibiotic prophylaxis is one of the main modifiable risk factors. We prospectively investigated the epidemiology, risk factors, and prognosis of SSI in a cohort of 167 OLT. Two different schedu...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181735407
更新日期:2008-06-27 00:00:00
abstract::Living-donor liver transplantation has provided a solution to the severe lack of cadaveric grafts for the replacement of livers afflicted with end-stage cirrhosis, fulminant disease, or inborn errors of metabolism. The pioneering experience in Japan in the early 1990s helped open wide the avenues of a new branch of sc...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.TP.0000046533.93621.C7
更新日期:2003-02-15 00:00:00
abstract::Publicly available program-specific data from the scientific registry of transplant recipients were used to determine the association between adult lung transplant center volume and 1-year recipient mortality from 2000 to 2007. We found a significant inverse association between the center volume of adult lung transpla...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181ceecf7
更新日期:2010-03-27 00:00:00
abstract:BACKGROUND:Osteoarthritis is the most prevalent joint disease and a frequent cause of joint pain, functional loss, and disability. Osteoarthritis often becomes chronic, and conventional treatments have demonstrated only modest clinical benefits without lesion reversal. Cell-based therapies have shown encouraging result...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318291a2da
更新日期:2013-06-27 00:00:00
abstract::Histoincompatible-complete radiation chimeras, after resolving acute graft-versus-host disease (GVHD), establish specific tolerance to host and donor alloantigens. This tolerance can be perturbed with immunosuppressive agents and infusions of small numbers of donor-type cells, with infusions of massive numbers of dono...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198109000-00005
更新日期:1981-09-01 00:00:00
abstract::The improvement of graft survival over the past decade has mainly been due to the development of more highly specific immunosuppressive agents, such as cyclosporine (CsA) and FK506. CsA and FK506 inhibit T cell activation by interfering with the calcium-mediated pathway, one of two pathways needed for T cell activatio...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199606150-00015
更新日期:1996-06-15 00:00:00
abstract::Blocking activity of mouse pregnancy serum was investigated using a well defined one-way mixed lymphocyte culture and a cell-mediated microcytotoxicity test. Allogeneic (C57BL (H-2b) X CBA/Ca (H-2k)) and syngeneic (C57BL (H-2b) X C57BL (H-2b)) pregnancy and postpartum sera were found to stimulate rather than inhibit t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197811000-00002
更新日期:1978-11-01 00:00:00
abstract:BACKGROUND:In this study, we investigated the effects of selective endothelin (ET) receptor antagonism during different periods of cold ischemia on glomerular and tubular function and long-term survival in renal transplantation. METHODS:Left renal transplantation was performed in Lewis rats after 2 hr of cold ischemia...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200103150-00007
更新日期:2001-03-15 00:00:00
abstract::Thirteen renal allograft recipients (6 patients on cyclosporine/7 patients on azathioprine) participated in a short-term oral protein-loading study to determine the effect of cyclosporine on an allograft's functional reserve. The baseline glomerular filtration rate of the two groups were similar (52 +/- 18 ml/min/1.73...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199101000-00030
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Although graft local infusion (GLI) treatment via the portal vein or the hepatic artery has been the pivotal strategy in ABO incompatible (ABOi) living donor liver transplantation (LDLT) in Japan, the procedure is associated with a high rate of catheter-associated complications. METHODS:A new ABOi-LDLT prot...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181adcae6
更新日期:2009-08-15 00:00:00
abstract:BACKGROUND:Current histological methods cannot accurately determine the survival rate of human pancreatic islets following portal vein infusion. This is due, in part, to the low number of infused islets relative to the whole liver. In this study, we assessed the ability of confocal laser scanning microscopy (CLSM) to t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000003191
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Our goal was to test the intragraft mRNA expression and production of two chemokines that are potent chemoattractants for antigen-primed T cells, interferon-gamma inducible protein 10 (IP-10) and monokine-induced by IFN-gamma, (Mig), in allogeneic heart grafts. METHODS:Syngeneic or allogeneic A/J (H-2a) hea...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200003270-00020
更新日期:2000-03-27 00:00:00
abstract:BACKGROUND:The prevalence of renal posttransplantation amputation and its impact on allograft and patient survival have not been widely reported. METHODS:We used an incident cohort of patients who underwent renal transplantation between June 2004 and September 2009. Amputation data were obtained using Medicare institu...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31828e72b4
更新日期:2013-05-27 00:00:00
abstract::A technique for simplified heterotopic rat heart transplantation having only aorto-aortic anastomosis is presented. The heterotopic rt heart survives and functions well when one lung lobe is attached t the transplantation and functions as a reservoir. Iso- and allotransplants are compared by electrocardiogram (ECG) de...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198204000-00020
更新日期:1982-04-01 00:00:00
abstract::Antigenic requirements for the induction of T cell-independent primary splenic IgM antibody responses (plaque-forming cell responses) to H-2Dd alloantigens were studied. Results show that some functional activity or structural property of the donor cells is required for immunogenicity, because antigens are not active ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198302000-00014
更新日期:1983-02-01 00:00:00
abstract:BACKGROUND:Hepatocellular carcinoma (HCC) is the most common primary malignant liver tumor. Currently, liver transplantation may be the optimal treatment for HCC in cirrhotic patients. Patient selection is currently based on tumor size. We developed a program to offer liver transplantation to selected patients with HCC...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002835
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:In the acute rejection of allografts, the interaction between Fas (CD95) and its ligand (FasL; CD95L) has been shown to be involved in mediating apoptotic cell death. The role, however, of these molecules in the pathogenesis of transplant vascular sclerosis is as yet undetermined. The present study was there...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199905270-00001
更新日期:1999-05-27 00:00:00