Abstract:
:The prostate gland of humans and other animals has the unique function of accumulating and secreting extraordinarily high levels of citrate. The prostate secretory epithelial cells synthesize citrate which, due to a limiting mitochondrial (m-) aconitase, accumulates rather than being oxidized. Thus citrate is essentially an end product of metabolism in prostate. For continued net citrate production, a continual source of oxaloacetate (OAA) and acetyl CoA is required. Glucose via pyruvate oxidation provides the source of Acetyl CoA. In prostate cells, citrate production is regulated by testosterone and/or by prolactin. Both hormones selectively regulate the level and activity of pyruvate dehydrogenase E1 alpha (E1a) in animal prostate cells; thereby regulating the availability of acetyl CoA for citrate synthesis. Studies were conducted to determine if testosterone and prolactin might regulate the expression of the E1a gene in prostate epithelial cells. Prolactin treatment of rat ventral and lateral prostate cells and human PC3 cells increased the levels of E1a mRNA and the rates of transcription of the E1a gene. Testosterone also increased the mRNA level and transcription of E1a in rat ventral prostate cells, and in PC3 cells transfected with androgen receptor. However, testosterone treatment resulted in a repression of E1a gene expression in lateral prostate cells. Evidence is presented which supports the view that prolactin regulation of E1a is mediated via PKC. The rapidity of the effects of both hormones is representative of an immediate-early gene response. To our knowledge this represents the first report in any mammalian cells that, in addition to its constitutive expression in all mammalian cells, the E1a gene is a hormonally-regulated gene in specifically targeted prostate epithelial cells.
journal_name
Endocr Resjournal_title
Endocrine researchauthors
Costello LC,Liu Y,Zou J,Franklin RBdoi
10.1080/07435800009040143subject
Has Abstractpub_date
2000-02-01 00:00:00pages
23-39issue
1eissn
0743-5800issn
1532-4206journal_volume
26pub_type
杂志文章abstract:PURPOSE:Adiponectin and chemerin have been reported their associations with insulin resistance and chronic inflammation. However, the relationship between adiponectin and chemerin themselves has not been fully elucidated. Therefore, we investigated the effects of changes in adiponectin and chemerin levels after a weigh...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2017.1300808
更新日期:2017-11-01 00:00:00
abstract::Activation of calcium-activated, phospholipid-dependent protein kinase C by phorbol esters such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA) has been shown to mediate release of hormones in many systems. To investigate the role of protein kinase C in the mechanism of pituitary ACTH release, we studied the effect of ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808809032983
更新日期:1988-01-01 00:00:00
abstract:PURPOSE:Primary hyperparathyroidism (PHPT) has been associated with increased incidence of morbidity and mortality of the cardiovascular system. The pathogenetic mechanisms underlying this association are still not completely clear. A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS) play a cri...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2017.1368543
更新日期:2018-02-01 00:00:00
abstract::We sought to determine the effect of maternal infections on the fetal hypothalamic-pituitary-adrenal axis. Umbilical cord blood was collected at vaginal delivery after labor (24-44 wk. gestation) from 361 infants of women having normal pregnancy ( apart from preterm delivery in some) and 110 infants of women diagnosed...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809909066145
更新日期:1999-08-01 00:00:00
abstract::Purpose/aim of the study: Graves' ophthalmopathy (GO) is closely related to the thyroid autoimmune disorder Graves' disease. Previous studies have suggested roles for thyroidal CD8+ T cells and autoimmunity against calsequestrin-1 (CASQ)-1 in the link between thyroidal and orbital autoimmune reactions in GO. A role fo...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2018.1470639
更新日期:2018-11-01 00:00:00
abstract:PURPOSE:Increasing evidence exists that hyperprolactinemia alters metabolic profile. The mechanism of this effect is unknown. We aimed to investigate the differences between the metabolic profile of patients with prolactinomas and nonfunctional pituitary adenomas and to evaluate the impact of other pituitary hormones o...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1135444
更新日期:2016-08-01 00:00:00
abstract:AIM:To evaluate, retrospectively, the frequency of antithyroid antibodies (ATA) in patients with type 1 diabetes (T1D). MATERIALS AND METHODS:Antithyroperoxidase antibodies (TPO-Ab), antithyroglobulin antibodies (TG-Ab), and antithyroid-stimulating hormone receptor antibodies (TSHR-Ab) were determined by enzyme-linked...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2011.639318
更新日期:2012-01-01 00:00:00
abstract::Several factors including genetic and environmental play a role in the development of obesity and the metabolic syndrome. The transgenic mouse overexpressing 11beta-hydroxysteroid dehydrogenase (11beta-HSD) develops visceral obesity. However, it remains unclear how a ubiquitously expressed 11beta-HSD1 enzyme affects a...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1081/erc-120026947
更新日期:2003-11-01 00:00:00
abstract::The effects of forskolin on the regulation of steroidogenesis and growth were examined in the Y1 adrenocortical tumor cell line, and the roles of cAMP and cAMP-dependent protein kinase in these actions of forskolin were evaluated. Forskolin, like corticotropin, stimulated steroidogenesis 3-fold and inhibited growth by...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808509032978
更新日期:1985-01-01 00:00:00
abstract::Progesterone (P) given intramuscularly increases renal sodium excretion. We tested the in vitro capacity of P to bind to the human mineralocorticoid receptor (MR) with a reticulocyte lysate system and Ps transactivation potency in transfected CV-1 cells. Progesterone binds with higher affinity to the MR than aldostero...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120016824
更新日期:2002-11-01 00:00:00
abstract::The effect of clomiphene on nuclear estrogen receptors of the Fallopian tube during ovum transport in the rabbit has been studied. Nuclear binding capacity was observed in ampulla (A), ampullary-isthmic junction (AIJ), isthmus (I), uterine-isthmic junction (UIJ) and uterus (U). Receptor concentration decreased in all ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808909042745
更新日期:1989-01-01 00:00:00
abstract::Diabetes mellitus impairs ventricular function, which itself may be disparately influenced by gender. This study compared the impact of gender on cardiac contractile response in ventricular myocytes from wild-type FVB and calmodulin-induced diabetic transgenic (OVE26) mice at young (2 month) and older (11 month) age. ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120022318
更新日期:2003-05-01 00:00:00
abstract:INTRODUCTION:The scavenger receptor class B type I (SR-BI) is a key component in the reverse cholesterol transportation. The aim of this study was to assess the association between exon1 (G → A) polymorphism of SR-BI gene and lipid profiles among the Tehran Lipid and Glucose Study (TLGS) population. MATERIALS AND METH...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.808207
更新日期:2014-01-01 00:00:00
abstract::Y1 adrenocortical tumor cells possess amplified and overexpressed c-Ki-ras proto-oncogene, displaying chronic high levels of the c-Ki-Ras-GTP protein. Despite this oncogenic lesion, we previously reported that Y1 cells retain tight regulatory mechanisms of cell cycle control typified by the mitogenic response triggere...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200043600
更新日期:2004-11-01 00:00:00
abstract::In order to clarify the roles of cholinergic and serotonergic neurotransmission in the mediation of exercise-induced growth hormone (GH) release, normal young volunteers of both sexes were studied. Exercise was for 20 minutes at 800 kpm for the men and 500 kpm for the women. Pretreatment with 0.4 mg atropine 1 hour pr...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035433
更新日期:1986-01-01 00:00:00
abstract:PURPOSE:Thyroglobulin (Tg) assessment in the needle washout after fine-needle aspiration biopsy (FNAB) of a suspicious neck lymph node (LN) is known to improve the diagnostic accuracy in patients with papillary thyroid cancer (PTC). However, there is still controversy on the best diagnostic cut-off levels for FNAB-Tg a...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2016.1141936
更新日期:2016-11-01 00:00:00
abstract::The action of peptide hormones from the anterior pituitary regulates transcription of a large number of genes located in most, if not all, tissues. This action is mediated through regulation of steroid hormone production in the steroidogenic factories (adrenals, gonads). These steroid hormones are transported through ...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1080/07435809609043755
更新日期:1996-11-01 00:00:00
abstract::In order to study the role of Platelet Activating Factor (PAF) in the mechanism of parturition and connective tissue metabolism in the pregnant rat uterus, we investigated the influence of the Platelet Activating Factor Receptor Antagonist (BN 52021) on gestation duration and collagen concentration in pregnant rat ute...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809409030414
更新日期:1994-11-01 00:00:00
abstract::We have previously reported that the protein kinase C ligand 12-O-tetradecanoyphorbol-13-acetate (TPA) inhibited the angiotensin II (AII) stimulated CYP11B2 gene expression in the adrenocortical H295R cell line. Here we report that TPA increased the level of phospho-p44/42 MAPK but AII did not. The MEK1 inhibitor PD98...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200044048
更新日期:2004-11-01 00:00:00
abstract::TRH is negatively regulated by T3 both in the hypothalamic paraventricular nucleus and transient transfection models. Mutations in hTR beta 1 genes are associated with the syndrome of generalized resistance to thyroid hormone. To investigate potential effects of mutant TRs on T3 regulation of the hTRH gene, transient ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809709031859
更新日期:1997-11-01 00:00:00
abstract::Tumor-promoting phorbol esters are believed to affect cell functions by activating a Ca+2-and lipid-dependent protein kinase (protein kinase C). 12-0-Tetradecanoyl phorbol-13-acetate (TPA) inhibits LH stimulation of progesterone (P) accumulation in rat granulosa cells. To determine the mechanisms by which TPA inhibits...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035438
更新日期:1986-01-01 00:00:00
abstract::We studied whether the activation of rat brown adipose tissue (BAT) by cold exposure or by the administration of beta-3-noradrenergic agonist CGP-12177 could be prevented by the inhibition of thyroxine (T4) to triiodothyronine (T3) conversion. Hypothyroid rats were treated with replacement doses of T4, T4 plus iopanoi...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009066164
更新日期:2000-05-01 00:00:00
abstract::The adrenal cortex is a complex tissue comprising different cell types, including endocrine, stromal, nerve, perivascular, and endothelial cells. The pituitary hormone ACTH controls the coordinated development of the vasculature and the endocrine tissue mass. This suggests that paracrine secretions between steroidogen...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200043623
更新日期:2004-11-01 00:00:00
abstract::Interleukin-23 receptor (IL-23R) and signal transducer and activator of transcription 3 (STAT3) polymorphisms are common risk factors for a number of T helper (Th) 17-mediated autoimmune diseases. However, the importance of genetic variations in Th17 pathways to thyroid autoimmunity, and particularly Hashimoto's thyro...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.875038
更新日期:2014-01-01 00:00:00
abstract::Congenital adrenal hyperplasia due to 21-hydroxylase (21-OH) deficiency is HLA-linked. The haplotype HLA-(A3);Bw47;DR7 is strongly associated with 21-OH deficiency and always carries a null allele at the complement C4A (Rodgers) locus. It seemed likely that this haplotype carries a deletion encompassing both the C4A a...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808409036505
更新日期:1984-01-01 00:00:00
abstract::The rat adrenal cortex possesses at least two mechanisms for uptake of extracellular lipoprotein cholesterol; one is receptor mediated endocytosis and the second is a non-endocytotic pathway. The latter was revealed by the ability of lipoproteins such as HDL which do not possess apo B or apo E to enhance steroid hormo...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1080/07435808409036509
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND:It has been suggested that the changes in hormone levels that occur with menopause and aging are related to loss of muscle mass. However, the role of gonadotropins in this process is unknown. OBJECTIVES:To analyze the relationship between follicle-stimulating hormone (FSH) and luteinizing hormone (LH) and l...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2012.733987
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND:Ultrasonography is considered useful to distinguish between solid and cystic thyroid nodules and to stratify a nodule's risk of cancer as low, medium, or high. Ultrasound (US) elastography has been applied to study the hardness/elasticity of nodules to differentiate malignant from benign lesions. Elastograph...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2011.633952
更新日期:2012-01-01 00:00:00
abstract::A comparative study (n = 20) of serum levels of leutinizing hormone (LH) and testosterone (T) between male trained athletes (high-school students, 16-18 years old) living in North and South Shouna in the Jordan Valley (JV), 320-360 meters below sea level, and those living in Ramtha and Irbid, 550-650 meters above sea ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-100107165
更新日期:2001-02-01 00:00:00
abstract::The mechanisms by which SF-1 (Steroidogenic Factor-1) and Dax-1 (Dosage-sensitive sex reversal-Adrenal hypoplasia congenita critical region on the X chromosome) dictate adrenal-specific transcriptional programs are the focus of this laboratory. SF-1-mediated transcription is upregulated by phosphorylation of serine 20...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009048628
更新日期:2000-11-01 00:00:00