Abstract:
:We have previously reported that the protein kinase C ligand 12-O-tetradecanoyphorbol-13-acetate (TPA) inhibited the angiotensin II (AII) stimulated CYP11B2 gene expression in the adrenocortical H295R cell line. Here we report that TPA increased the level of phospho-p44/42 MAPK but AII did not. The MEK1 inhibitor PD98059 was found to increase the level of aldosterone synthase mRNA and the activity of a human CYP11B2(-2023 bp)-promoter construct. The cotransfection of H295R with ERK 1 and the hCYP11B2 promoter resulted in the inhibition of the promoter activity. TPA but not AII increased the level of the transcription factor JunB in nuclear extracts and the increase was partially abolished by the MEK1 inhibitor PD98059. The cotransfection of H295R with JunB and the hCYP11B2 promoter abolished the AII stimulating effect. Taken together these results suggest that TPA inhibits the AII-dependent activation of CYP11B2 via the p44/42 MAPK signaling pathway leading to an increase of the level of nuclear JunB.
journal_name
Endocr Resjournal_title
Endocrine researchauthors
LeHoux JG,Lefebvre Adoi
10.1081/erc-200044048subject
Has Abstractpub_date
2004-11-01 00:00:00pages
807-12issue
4eissn
0743-5800issn
1532-4206journal_volume
30pub_type
杂志文章abstract::Dehydroepiandrosterone sulphate (DHEAS) is a steroid product of the adrenal gland, which circulates in high concentrations, but whose functions are largely unknown. There is evidence for antiproliferative effects of DHEAS in neoplastic tissue. The present study was designed to investigate the effects of DHEAS on vascu...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200043956
更新日期:2004-11-01 00:00:00
abstract::Thyroid dysfunction influences the levels of Von Willebrand factor (VWF:Ag). Plasma VWF:Ag levels in 35 hyperthyroid patients were significantly elevated, which had a positive correlation with both T3 (Triiodothyronine) and T4 (Thyroxine) levels. A follow-up study of seven hyperthyroid patients undergoing anti-thyroid...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3109/07435809309033019
更新日期:1993-01-01 00:00:00
abstract::Addition of cAMP (as the dibutyryl compound) to a primary culture of mouse Leydig cells caused the accumulation of a protein, i(b), with the same dependence on cAMP concentration as the increase in testosterone synthesis. Stimulation of both protein i(b) and testosterone production were inhibited by cycloheximide. Add...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808609035449
更新日期:1986-01-01 00:00:00
abstract::We sought to determine the effect of maternal infections on the fetal hypothalamic-pituitary-adrenal axis. Umbilical cord blood was collected at vaginal delivery after labor (24-44 wk. gestation) from 361 infants of women having normal pregnancy ( apart from preterm delivery in some) and 110 infants of women diagnosed...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809909066145
更新日期:1999-08-01 00:00:00
abstract::Two distinct isoforms of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) with respect to enzymatic activity were identified in the ovine liver and kidney. 11 beta-HSD1 (the hepatic isoform) was reversible and NADP(H)-dependent. By contrast, 11 beta-HSD2 (the renal isoform) was unidirectional and NAD-dependent. Ovin...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.3109/07435809509030453
更新日期:1995-02-01 00:00:00
abstract::The zonal distribution of aldosterone synthase cytochrome P450 (P450aldo) in the adrenal cortex of male hamsters was investigated by immunofluorescence and electron microscopy, using an anti-P450aldo peptide antibody. On cryostat sections the immunolocalization of P450aldo was confined to the zona glomerulosa cells. O...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030443
更新日期:1995-02-01 00:00:00
abstract:INTRODUCTION:The scavenger receptor class B type I (SR-BI) is a key component in the reverse cholesterol transportation. The aim of this study was to assess the association between exon1 (G → A) polymorphism of SR-BI gene and lipid profiles among the Tehran Lipid and Glucose Study (TLGS) population. MATERIALS AND METH...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.808207
更新日期:2014-01-01 00:00:00
abstract::Tumor-promoting phorbol esters are believed to affect cell functions by activating a Ca+2-and lipid-dependent protein kinase (protein kinase C). 12-0-Tetradecanoyl phorbol-13-acetate (TPA) inhibits LH stimulation of progesterone (P) accumulation in rat granulosa cells. To determine the mechanisms by which TPA inhibits...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035438
更新日期:1986-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:Glucose tolerance progressively declines with age, and there is a high prevalence of type 2 diabetes in the elderly people. Previous studies have reported the sex differences in risk for type 2 diabetes, especially in the elderly people, whereas reasons for these sex differences remain poorly understood...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1124437
更新日期:2016-08-01 00:00:00
abstract::The effect of crowding on the pituitary-gonadal axis was studied in adult male rats. Crowding reduced body weight gain, did not alter relative adrenal weight and increased relative testis weight. Neither basal levels of corticosterone nor its response to acute stress were altered by crowding. Likewise, LH secretion wa...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035431
更新日期:1986-01-01 00:00:00
abstract::Using Western blot analysis we found transcriptional co-repressor Sin3A to be expressed at a higher level in male mouse kidney than in females. HDAC1 (histone deacetylase 1) protein, another co-repressor forming complexes with Sin3A, was not higher in males. No sex differences in Sin3A expression were found after gona...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800500229243
更新日期:2005-01-01 00:00:00
abstract::Type 1 angiotensin II (AII) receptors (AT1 receptors), besides stimulation of aldosterone secretion, seem to transduce the growth factor-like activity of AII on glomerulosa cells. Although a local renin-angiotensin system and AII synthesis have been found in human adrenals and aldosteronomas, it is unclear whether ald...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030434
更新日期:1995-02-01 00:00:00
abstract:AIM:To determine whether hyperuricemia is associated with the early phase and late phase of insulin release in apparently healthy non-obese subjects with normal glucose tolerance. MATERIALS AND METHODS:A population-based cross-sectional study was performed. Apparently healthy individuals aged 20 to 65 years were eligi...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2016.1155597
更新日期:2017-02-01 00:00:00
abstract::A method is described for the preparation of high yields of viable, dissociated cells from porcine theca interna and corpus luteum and from human and bovine endometrium. The tissues were dissociated by incubation at 37 degrees C in a mixture of 0.5% collagenase, 0.1% hyaluronidase and 0.1% pronase in balanced salt sol...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808409035415
更新日期:1984-01-01 00:00:00
abstract::We quantitated serial serum beta-hydroxybutyrate (beta-OHB) levels using the Ketosite method in 9 children with IDDM who were treated for diabetic ketoacidosis (DKA) and compared them to urinary ketone measurements by dipstick. Persistent elevations of serum beta-OHB were seen in six patients when the urine became cle...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-100107168
更新日期:2001-02-01 00:00:00
abstract::The adrenal cortex is a dynamic organ in which the cells of the outer cortex continually divide. It is well known that this cellular proliferation is dependent on constant stimulation from peptides derived from the ACTH precursor proopiomelanocortin (POMC) since disruption of pituitary corticotroph function results in...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120016801
更新日期:2002-11-01 00:00:00
abstract::Several factors including genetic and environmental play a role in the development of obesity and the metabolic syndrome. The transgenic mouse overexpressing 11beta-hydroxysteroid dehydrogenase (11beta-HSD) develops visceral obesity. However, it remains unclear how a ubiquitously expressed 11beta-HSD1 enzyme affects a...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1081/erc-120026947
更新日期:2003-11-01 00:00:00
abstract::Purpose: Transthyretin (TTR) is a protein with a growing number of biological functions in addition to its well-established binding and circulatory transport of thyroxine, and indirect retinoid transport through interaction with retinol-binding protein. Misfolded and aggregated wild-type and mutant TTRs are involved i...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2019.1694538
更新日期:2020-01-01 00:00:00
abstract::We report a novel mutant of the luteinizing hormone receptor (LHR) in a case of familial Leydig cell hypoplasia and pseudohermaphrotidism. The proband was homozygous for two missense mutations, T1121C and C1175T, causing substitutions I374T and T3921. The molecular effects of the mutations were investigated by heterol...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800500430890
更新日期:2005-01-01 00:00:00
abstract::The enzyme 3beta-hydroxysteroid dehydrogenase plays a crucial role in the steroidogenic process in the adrenal gland. In the present study we tried to characterize its localization and developmental changes in the rat adrenal cortex during the postnatal period, using immunohistochemical methods. The development of the...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809909066126
更新日期:1999-02-01 00:00:00
abstract:PURPOSE:Primary hyperparathyroidism (PHPT) has been associated with increased incidence of morbidity and mortality of the cardiovascular system. The pathogenetic mechanisms underlying this association are still not completely clear. A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS) play a cri...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2017.1368543
更新日期:2018-02-01 00:00:00
abstract::Metabolic syndrome was first proposed in 1988 and has been recognized as a powerful predictor for cardiovascular disease and diabetes. At the same time, white blood cell count (WBCC) was proposed to have significant correlation with metabolic syndrome (MS). In this study, we attempted to investigate the relationship b...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800500229151
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:Tetraiodothyroacetic acid (tetrac) and its nanoparticulate formulation (Nanotetrac) act at a cell surface receptor to block angiogenesis and tumor cell proliferation. OBJECTIVE:The complex anti-angiogenic properties of tetrac and Nanotetrac caused us to search in the literature and in certain of our unpubli...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.3109/07435800.2013.778865
更新日期:2013-01-01 00:00:00
abstract::11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) catalyzes the interconversion of cortisol (F) to inactive cortisone (E) in man (corticosterone (B) to 11-dehydrocorticosterone (A) in rodents) and plays a crucial role in regulating corticosteroid hormone action. Two isoforms of this enzyme have been characterized; a ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809609043775
更新日期:1996-11-01 00:00:00
abstract::The present work was to study if the alpha-melanocyte-stimulating hormone (alpha-MSH) was involved in activation of the pituitary-adrenal axis (PAA) in rats. The hormone increased plasma corticosterone (CORT) level, and induced an anxiogenic response as indicated by results from the elevated plus-maze test. Intracereb...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009066172
更新日期:2000-08-01 00:00:00
abstract:PURPOSE:Adiponectin and chemerin have been reported their associations with insulin resistance and chronic inflammation. However, the relationship between adiponectin and chemerin themselves has not been fully elucidated. Therefore, we investigated the effects of changes in adiponectin and chemerin levels after a weigh...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2017.1300808
更新日期:2017-11-01 00:00:00
abstract::In order to clarify the roles of cholinergic and serotonergic neurotransmission in the mediation of exercise-induced growth hormone (GH) release, normal young volunteers of both sexes were studied. Exercise was for 20 minutes at 800 kpm for the men and 500 kpm for the women. Pretreatment with 0.4 mg atropine 1 hour pr...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035433
更新日期:1986-01-01 00:00:00
abstract::In a previous study we demonstrated a significant increase of CD5+ B subset in patients with Graves' disease (GD) compared with normal controls. The aim of this study was to compare the percentage of CD5+ B and CD5- B cells in GD with that in different forms of autoimmune and non immune-mediated thyroid diseases. Seve...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809709031844
更新日期:1997-02-01 00:00:00
abstract:PURPOSE:Monthly regimen of minodronate for osteoporosis more than two years has not been reported yet. The aim of this study is to elucidate the effect of monthly minodronate (M-MIN) on bone mineral density (BMD) and serum pentosidine (Pen) during 27 months. MATERIALS AND METHODS:The study consisted of 52 newly treate...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2017.1292527
更新日期:2017-08-01 00:00:00
abstract::CYP17 is not expressed in adult mouse adrenal glands but is expressed in a subset of fetal adrenocortical cells, indicating the potential to produce both corticosterone and cortisol during murine embryogenesis. CYP11A is expressed in the fetal adrenal but also in developing hindgut, which will form the colon, and in c...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030425
更新日期:1995-02-01 00:00:00