Abstract:
:Purpose: Transthyretin (TTR) is a protein with a growing number of biological functions in addition to its well-established binding and circulatory transport of thyroxine, and indirect retinoid transport through interaction with retinol-binding protein. Misfolded and aggregated wild-type and mutant TTRs are involved in amyloid diseases. Several aspects of TTR pathology and physiology remain poorly understood. Receptor-mediated cellular transport of TTR has been described in a few cell types; and such studies suggest the possibility of different TTR receptors and endocytic pathways. Our main objective was to further understand the endocytic pathways for TTR.Methods: In the current study, analyses of TTR endocytic transport were performed in the human A431 cell line. The results of TTR uptake were compared with those of the iron-carrier protein transferrin (Tf, a common stardard for endocytosis studies) in the same cell types.Results: A comparison of TTR and Tf endocytosis suggested similar early, 5-10 min, accumulation kinetics. But at a later time, 30 min, TTR accumulation was 20-30% lower than that of Tf (p < .05), a result that suggests different post-endocytic fates for these two ligands. Through the use of multiple endocytosis inhibitors, biochemical evidence is provided for an internalization pathway that differs from the clathrin-mediated endocytosis of Tf.Conclusions: These results for A431 cells are compared with others reported for different cell types; and it is suggested that this same hormone carrier protein can transit into cells through multiple endocytic pathways.
journal_name
Endocr Resjournal_title
Endocrine researchauthors
Fong VH,Wong S,Jintaridhi P,Vieira Adoi
10.1080/07435800.2019.1694538subject
Has Abstractpub_date
2020-01-01 00:00:00pages
131-136issue
2eissn
0743-5800issn
1532-4206journal_volume
45pub_type
杂志文章abstract::The present work was to study if the alpha-melanocyte-stimulating hormone (alpha-MSH) was involved in activation of the pituitary-adrenal axis (PAA) in rats. The hormone increased plasma corticosterone (CORT) level, and induced an anxiogenic response as indicated by results from the elevated plus-maze test. Intracereb...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009066172
更新日期:2000-08-01 00:00:00
abstract::We quantitated serial serum beta-hydroxybutyrate (beta-OHB) levels using the Ketosite method in 9 children with IDDM who were treated for diabetic ketoacidosis (DKA) and compared them to urinary ketone measurements by dipstick. Persistent elevations of serum beta-OHB were seen in six patients when the urine became cle...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-100107168
更新日期:2001-02-01 00:00:00
abstract::StAR increases the flow of cholesterol from the outer to inner mitochondrial membrane (OMM to IMM), but its mechanism of action remains unclear. MLN64 is a 445 amino acid protein of unknown function that has four N-terminal transmembrane domains and whose C-terminal domain from 218-445 is 37% identical to StAR. N-62 S...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009048583
更新日期:2000-11-01 00:00:00
abstract:AIM:To evaluate, retrospectively, the frequency of antithyroid antibodies (ATA) in patients with type 1 diabetes (T1D). MATERIALS AND METHODS:Antithyroperoxidase antibodies (TPO-Ab), antithyroglobulin antibodies (TG-Ab), and antithyroid-stimulating hormone receptor antibodies (TSHR-Ab) were determined by enzyme-linked...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2011.639318
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVES:The diagnosis and management of osteoporosis and osteoporotic fractures are challenging in rural and underdeveloped areas of China because medical resources are inaccessible; thus, a simple and accurate method is essential for the detection of vertebral fractures. We aimed to examine the relationship between...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2020.1827263
更新日期:2021-02-01 00:00:00
abstract::Progesterone (P) given intramuscularly increases renal sodium excretion. We tested the in vitro capacity of P to bind to the human mineralocorticoid receptor (MR) with a reticulocyte lysate system and Ps transactivation potency in transfected CV-1 cells. Progesterone binds with higher affinity to the MR than aldostero...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120016824
更新日期:2002-11-01 00:00:00
abstract:PURPOSE:Monthly regimen of minodronate for osteoporosis more than two years has not been reported yet. The aim of this study is to elucidate the effect of monthly minodronate (M-MIN) on bone mineral density (BMD) and serum pentosidine (Pen) during 27 months. MATERIALS AND METHODS:The study consisted of 52 newly treate...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2017.1292527
更新日期:2017-08-01 00:00:00
abstract::The enzyme, 3 beta-hydroxysteroid dehydrogenase/delta 5-4 isomerase (3 beta-HSD) is an essential element in the biosynthetic pathway for potent adrenal steroid hormones that appear to regulate maturation of many tissues in utero and are critical for homeostasis after birth. The results of prior studies are suggestive ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030422
更新日期:1995-02-01 00:00:00
abstract:PURPOSE:This retrospective and observational study aimed to establish whether any relationship exists between the values of serum thyroid-stimulating hormone (TSH) and those of the mean platelet volume (MPV) in a large cohort of older euthyroid Italian subjects. MATERIALS AND METHOD:The study population was represente...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1037392
更新日期:2015-01-01 00:00:00
abstract::The presence of vasoactive intestinal peptide and neuropeptide Y in thyroid nerves and their effects on thyroid blood flow are well known. However, the effects of these two neuropeptides on the various processes involved in thyroid hormone biosynthesis and release have not been fully explored. We have now tested these...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809209026678
更新日期:1992-01-01 00:00:00
abstract::Congenital adrenal hyperplasia due to 21-hydroxylase (21-OH) deficiency is HLA-linked. The haplotype HLA-(A3);Bw47;DR7 is strongly associated with 21-OH deficiency and always carries a null allele at the complement C4A (Rodgers) locus. It seemed likely that this haplotype carries a deletion encompassing both the C4A a...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808409036505
更新日期:1984-01-01 00:00:00
abstract::Interleukin-23 receptor (IL-23R) and signal transducer and activator of transcription 3 (STAT3) polymorphisms are common risk factors for a number of T helper (Th) 17-mediated autoimmune diseases. However, the importance of genetic variations in Th17 pathways to thyroid autoimmunity, and particularly Hashimoto's thyro...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.875038
更新日期:2014-01-01 00:00:00
abstract::The CYP11B2 gene encodes aldosterone synthase, a cytochrome P450 (P450aldo) expressed in high levels in the adrenal zona glomerulosa. While the primary physiologic regulators of aldosterone production are circulating angiotensin II (Ang II) and potassium (K+) the action of these agents on CYP11B2 gene transcription ha...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809609043735
更新日期:1996-11-01 00:00:00
abstract::Two distinct isoforms of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) with respect to enzymatic activity were identified in the ovine liver and kidney. 11 beta-HSD1 (the hepatic isoform) was reversible and NADP(H)-dependent. By contrast, 11 beta-HSD2 (the renal isoform) was unidirectional and NAD-dependent. Ovin...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.3109/07435809509030453
更新日期:1995-02-01 00:00:00
abstract::Pretreatment of confluent human fibroblast cultures for two days in dexamethasone, serum free medium increased 10-20 fold the sensitivity of the cells to insulin-like growth factor I (IGF I) stimulation of amino acid uptake using the amino acid analog alpha-aminoisobutyric acid (AIB). This increased sensitivity result...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808909042743
更新日期:1989-01-01 00:00:00
abstract::Combined partial deficiency of 17alpha-hydroxylase and 21-hydroxylase activities was first described in 1985; however the genes for P450c17 and P450c21 in these patients lack mutations. In 1986 we postulated that this disorder might be due to mutations in P450 oxidoreductase (POR), the flavoprotein that donates electr...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200044134
更新日期:2004-11-01 00:00:00
abstract::The adrenal cortex is a dynamic organ in which the cells of the outer cortex continually divide. It is well known that this cellular proliferation is dependent on constant stimulation from peptides derived from the ACTH precursor proopiomelanocortin (POMC) since disruption of pituitary corticotroph function results in...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120016801
更新日期:2002-11-01 00:00:00
abstract:PURPOSE:Thyroglobulin (Tg) assessment in the needle washout after fine-needle aspiration biopsy (FNAB) of a suspicious neck lymph node (LN) is known to improve the diagnostic accuracy in patients with papillary thyroid cancer (PTC). However, there is still controversy on the best diagnostic cut-off levels for FNAB-Tg a...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2016.1141936
更新日期:2016-11-01 00:00:00
abstract::TRH is negatively regulated by T3 both in the hypothalamic paraventricular nucleus and transient transfection models. Mutations in hTR beta 1 genes are associated with the syndrome of generalized resistance to thyroid hormone. To investigate potential effects of mutant TRs on T3 regulation of the hTRH gene, transient ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809709031859
更新日期:1997-11-01 00:00:00
abstract::It is now well documented that obesity is associated with a chronic low-grade inflammatory state. Levels of high-sensitivity C-reactive protein, a marker of systemic inflammation and a mediator of atherothrombotic disease, have been shown to correlate with cardiovascular disease risk. Our objective was to evaluate the...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章
doi:10.1081/erc-200033541
更新日期:2004-08-01 00:00:00
abstract::We have recently reported on the activities of glucose-6-phosphate dehydrogenase (G6PD), hexokinase (HK) and carbonic anhydrase I (CA-I) and II (CA-II) isoenzymes obtained from erythrocytes of healthy subjects and untreated patients with hyperthyroid diseases. Also, erythrocyte zinc concentrations were measured. Red b...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120004538
更新日期:2002-02-01 00:00:00
abstract::Neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP) are both intrapancreatic neuropeptides that are known to inhibit stimulated insulin secretion. In the present study, we examined their influences on basal and stimulated glucagon and insulin secretion in the mouse. Either NPY or CGRP was injected intraven...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808709035466
更新日期:1987-01-01 00:00:00
abstract::The prostate gland of humans and other animals has the unique function of accumulating and secreting extraordinarily high levels of citrate. The prostate secretory epithelial cells synthesize citrate which, due to a limiting mitochondrial (m-) aconitase, accumulates rather than being oxidized. Thus citrate is essentia...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800009040143
更新日期:2000-02-01 00:00:00
abstract::The enzyme 3beta-hydroxysteroid dehydrogenase plays a crucial role in the steroidogenic process in the adrenal gland. In the present study we tried to characterize its localization and developmental changes in the rat adrenal cortex during the postnatal period, using immunohistochemical methods. The development of the...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809909066126
更新日期:1999-02-01 00:00:00
abstract::Type 1 angiotensin II (AII) receptors (AT1 receptors), besides stimulation of aldosterone secretion, seem to transduce the growth factor-like activity of AII on glomerulosa cells. Although a local renin-angiotensin system and AII synthesis have been found in human adrenals and aldosteronomas, it is unclear whether ald...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030434
更新日期:1995-02-01 00:00:00
abstract::Metabolic syndrome was first proposed in 1988 and has been recognized as a powerful predictor for cardiovascular disease and diabetes. At the same time, white blood cell count (WBCC) was proposed to have significant correlation with metabolic syndrome (MS). In this study, we attempted to investigate the relationship b...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800500229151
更新日期:2005-01-01 00:00:00
abstract::There are several published reports on the prevalence of low vitamin D levels in otherwise healthy Indian population. Vitamin D deficiency has shown variable effect on muscle performance and strength but there is paucity of data on the effect of vitamin D deficiency on muscle energy metabolism. The present study was p...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章
doi:10.3109/07435800.2013.865210
更新日期:2014-01-01 00:00:00
abstract::Immunohistochemistry using a StAR peptide antiserum had previously revealed strong staining in rat and bovine adrenal medulla, suggesting the presence of a protein immunogenically related to StAR. Western blots of bovine medulla tissue homogenates showed the principal adrenal medullary immuno-reactive species to have ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009048594
更新日期:2000-11-01 00:00:00
abstract::Comparison of binding of 125I-porcine FSH to porcine granulosa cells from small and large ovarian follicles indicated that binding is dependent on both time and temperature. At 37 C, binding of ligand is more than four times higher in cells from small follicles than in cells from large follicles within 30 minutes of t...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809409035857
更新日期:1994-02-01 00:00:00
abstract::In order to study the role of Platelet Activating Factor (PAF) in the mechanism of parturition and connective tissue metabolism in the pregnant rat uterus, we investigated the influence of the Platelet Activating Factor Receptor Antagonist (BN 52021) on gestation duration and collagen concentration in pregnant rat ute...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809409030414
更新日期:1994-11-01 00:00:00