Abstract:
:The Ro ribonucleoprotein particle (RoRNP) is the target of a variety of specific anti-protein autoantibodies produced by patients with systemic autoimmune diseases. RoRNPs, the function of which remains elusive, appear to be rather heterogeneous in terms of their molecular composition. Among several Ro proteins, a protein of 52 kD (Ro52) has been identified, but its association with RoRNPs remains questionable. In this study, we first mapped the Ro52 regions that are accessible at the surface of the isolated protein, by means of antibodies raised in rabbits, against 39 overlapping synthetic peptides covering the whole Ro52 molecule and using several different methods, including various ELISA formats and Western blotting (based on the use of recombinant Ro52) and immunoprecipitation of in vitro translated Ro52. Then, the whole set of anti-peptide antibodies was used to attempt to immunoprecipitate RoRNPs from a cell extract of K562 cells. RoRNPs, especially Ro(hY3)RNPs, were effectively immunoprecipitated by certain anti-peptide antibodies, indicating that Ro52 protein is associated with at least a subset of RoRNP particles. As expected, a number of epitopes available on the isolated Ro52 molecule are no longer accessible when Ro52 is associated with the RoRNP. Conversely, neotopes, among them at least one corresponding to a previously characterized epitope recognized by patients' antibodies, are only present at the surface of the particle and not detectable on the isolated Ro52 protein.
journal_name
J Autoimmunjournal_title
Journal of autoimmunityauthors
Ricchiuti V,Pruijn GJ,Thijssen JP,van Venrooij WJ,Muller Sdoi
10.1006/jaut.1996.0122subject
Has Abstractpub_date
1997-04-01 00:00:00pages
181-91issue
2eissn
0896-8411issn
1095-9157pii
S0896-8411(96)90122-5journal_volume
10pub_type
杂志文章abstract::Primary biliary cholangitis (PBC) is a classic autoimmune disease in which humoral, cytotoxic, and innate immune responses have been implicated with the specific targeting of a mitochondrial antigen. The mainstay of treatment remains the bile acid ursodeoxycholic acid (UDCA). Corticosteroids may have some benefits, bu...
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
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更新日期:2018-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-04-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:1992-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0896-8411(89)80002-2
更新日期:1989-10-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1006/jaut.1994.1040
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
abstract::Autoimmunity is a field that has only been around for a little over a century. Initially, it was thought that autoimmunity could not happen, that the body would never turn on itself (i.e. "horror autotoxicus"). It was only around the First World War that autoimmunity was recognized as the pathogenesis of various disea...
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