Abstract:
BACKGROUND:In cases where hyperacute rejection has been prevented, pig to primate organ transplantation results in a delayed rejection mediated by graft-infiltrating leukocytes. The migration of human leukocytes across porcine endothelium is poorly characterized, but may offer targets for species-specific antirejection therapy. METHODS:Transwell tissue culture inserts with endothelial cells growing on polycarbonate filters were used to characterize the migration of peripheral blood monocuclear cells and purified leukocyte subpopulations across pig and human endothelial cells and cell lines. Endothelial cell morphology was evaluated by scanning and transmission electron microscopy, and the contribution of different adhesion receptor pairs to transendothelial migration was evaluated by antibody blocking experiments. RESULTS:There were no evident quantitative or qualitative differences in the capacity of human and porcine endothelium to support transendothelial migration of human leukocytes [T, B, and natural killer (NK) cells, monocytes, and neutrophils]. Monocytes and large granular CD3+ lymphocytes migrated most efficiently across the endothelium. Antiporcine vascular cell adhesion molecule-1 antibodies blocked transendothelial migration of human monocytes and NK cells across tumor necrosis factor-alpha stimulated pig endothelium by at least 60%. Anti-CD18 antibodies had no effect on the migration of human NK cells across pig endothelium, whereas they partly blocked migration of NK cells across human endothelium and migration of monocytes across porcine endothelium. Interleukin-2 stimulated, but not unstimulated, T and NK cells were cytotoxic to porcine endothelium. CONCLUSIONS:Porcine endothelium supports transendothelial migration of human leukocyte subpopulations as efficiently as human endothelium. Incompatibilities in some adhesion receptor pairs may be compensated for by other adhesion receptor pairs, as exemplified by human NK cells whose migration across human, but not pig, endothelium was blocked by anti-CD18 antibodies. Antiporcine vascular cell adhesion molecule-1 antibodies may be used as species-specific blockers of transendothelial NK cell and monocyte migration, and as such may prove to be useful inhibitors of cellular organ xenograft rejection.
journal_name
Transplantationjournal_title
Transplantationauthors
Hauzenberger E,Hauzenberger D,Hultenby K,Holgersson Jdoi
10.1097/00007890-200005150-00018subject
Has Abstractpub_date
2000-05-15 00:00:00pages
1837-49issue
9eissn
0041-1337issn
1534-6080journal_volume
69pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Antibody-mediated rejection (AMR) is a leading cause of morbidity and mortality after kidney transplantation. Early diagnosis and treatment of subclinical AMR based on the donor-specific antibody (DSA) testing may result in better outcomes. METHODS:We tested this hypothesis in 220 kidney transplant recipien...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002566
更新日期:2019-08-01 00:00:00
abstract::We have demonstrated that DA hearts grafted into PVG rats were completely protected from rejection by simultaneous liver transplantation from the same donor. In subsequent experiments PVG animals were given DA hearts followed 5 or 6 days later by livers from the same donor strain. Instead of the expected rapid rejecti...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198409000-00004
更新日期:1984-09-01 00:00:00
abstract:BACKGROUND:It is unclear whether ischemic preconditioning (IPC) of solid organs induces remote IPC (RIPC) in donors after brain death (DBD). METHODS:Outcomes in kidney recipients from 163 DBD in two randomized trials of liver IPC (5 min=62 and 10 min=101) were obtained retrospectively from the Scientific Registry of T...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000436926.30897.56
更新日期:2014-02-15 00:00:00
abstract::A retrospective study of the 99 surviving heart and lung transplant (HLT) recipients at one center showed that 31% had significant anemia (hemoglobin less than 100g/L) six months after transplantation. Chronic anemia persisted in 18% of HLT recipients two years posttransplantation. A similar study of 100 heart transpl...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199206000-00016
更新日期:1992-06-01 00:00:00
abstract::A novel method for quantifying the fatty change of the graft liver by characterizing the optical property of the tissue was introduced. A wide range of lipid content in the rat liver was obtained by using different feeding regimens, with lipotropic chow (choline/methionine deficient or low chow). The liver was removed...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199609150-00018
更新日期:1996-09-15 00:00:00
abstract:BACKGROUND:AGI-1096 is a novel phenolic intracellular antioxidant with anti-inflammatory and antiproliferative properties. In vitro, AGI-1096 inhibited the inducible expression of vascular cell adhesion molecule (VCAM)-1, E-selectin, and monocyte chemoattractant protein (MCP)-1 in endothelial cells and tumor necrosis f...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000123076.05313.9f
更新日期:2004-05-27 00:00:00
abstract:BACKGROUND:It has been proposed that hyperacute rejection (HAR) of pig-to-primate vascularized xenografts is due in large part to ineffective regulation of recipient complement by pig complement regulatory proteins (CRPs), and indeed transgenic expression of human CRPs in pigs can prevent hyperacute rejection. However,...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200009270-00014
更新日期:2000-09-27 00:00:00
abstract::Until 1996, living kidney donation had been neglected in Germany. To encourage potential donors and to convince them that nephrectomy is not too risky for a healthy person, the author decided to set an example with a personal nondirected donation. This donation, controversially discussed in the public domain, neverthe...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000087834.02707.5B
更新日期:2003-10-27 00:00:00
abstract:BACKGROUND:The administration of systemic immunosuppressive agents to recipients of solid organ or bone marrow transplants results in an immunocompromised status. As the number of organ recipients and their life span increase with recent progress in organ transplantation, ocular complications tend to become more divers...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000259386.59375.8a
更新日期:2007-03-27 00:00:00
abstract:BACKGROUND:ABO-incompatible organ transplantation typically induces hyperacute rejection. A2-to-O liver transplantations have been successful. This study compared overall and graft survival in O recipients of A2 and O grafts based on Organ Procurement and Transplantation Network data. METHODS:Scientific Registry of Tr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31825c591e
更新日期:2012-09-15 00:00:00
abstract:BACKGROUND:The purpose of this study was to examine the utility of the random urine protein to creatinine ratio (P/C) in evaluation and longitudinal management of proteinuria in adult renal transplant recipients with or without overt nephropathy in an outpatient clinic. METHODS:A total of 289 adult renal transplant re...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200110270-00021
更新日期:2001-10-27 00:00:00
abstract:BACKGROUND:Matrix metalloproteinases (MMPs) play a key role in extracellular matrix remodeling events associated with hepatic regeneration after partial hepatectomy. We therefore hypothesized that urinary MMPs and their endogenous tissue inhibitors of matrix metalloproteinases (TIMPs) might also provide important infor...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000137935.81103.a2
更新日期:2004-10-27 00:00:00
abstract::Preexisting diabetes mellitus (DM) has been regarded as a contraindication to heart transplantation (HT). This prejudice has been based upon concern over increased infection rates and worsening DM with the initiation of prednisone immunosuppression. To better evaluate these suppositions, we reviewed our experience wit...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199002000-00015
更新日期:1990-02-01 00:00:00
abstract:BACKGROUND:To reduce the side effects of long-term immunosuppressive therapy, stable renal transplant patients were routinely converted from cyclosporine to either azathioprine or mycophenolate mofetil. Thereafter, the azathioprine and mycophenolate mofetil dose was reduced to 75% at 4 months and to 50% at 8 months aft...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:2000-07-15 00:00:00
abstract::Heterotopic renal allografts following bilateral nephrectomies were placed in 21 healthy mongrel dogs. One group of 11 dogs received cyclosporine (5 mg/kg/24 hr, orally), and 1 group of 10 dogs received cyclosporine and mizoribine (5 mg/kg and 3 mg/kg/24 hr, orally). Body weights, blood cell counts, serum chemistry pr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198805000-00003
更新日期:1988-05-01 00:00:00
abstract:BACKGROUND:The inflammatory reaction that occurs during the 5 days after transplantation led at 3 days to the death of 70% of injected myoblasts. Use of anti-inflammatory agents appeared to be a possible way to increase myoblast survival. The application of gene transfer techniques to cell transplantation offers the po...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199803270-00005
更新日期:1998-03-27 00:00:00
abstract::BALB/cxDBA/2Wf F1 (CD2F1) mice were lethally irradiated and reconstituted with syngeneic bone marrow cells untreated or treated with 75 micrograms/ml of mafosfamide. One day after bone marrow transplantation some groups of mice were injected with syngeneic splenocytes, peripheral blood leukocytes, or thymocytes. Seven...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199104000-00020
更新日期:1991-04-01 00:00:00
abstract:BACKGROUND:Patients with von Hippel-Lindau (VHL) disease are at risk for the development of end-stage renal failure from the treatment of localized renal cell carcinoma. Transplantation with its attendant immunosuppression may predispose patients to tumor recurrence; however, there is little information regarding the o...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199712270-00017
更新日期:1997-12-27 00:00:00
abstract::Shortage of organ donors limits the number of possible liver transplantations. Alternative therapies for treatment of liver failure are currently being developed: (i) extracorporeal artificial liver devices; (ii) bioartificial liver devices using hepatocytes; and (iii) hepatocyte transplantation. The objective of thes...
journal_title:Transplantation
pub_type: 社论,评审
doi:10.1097/TP.0b013e3181963ad3
更新日期:2009-02-27 00:00:00
abstract::The recent introduction of a variety of techniques for removing T cells from bone marrow grafts has reduced the incidence of graft-versus-host disease (GVHD)-associated morbidity and mortality. Whether this advance will be translated into improved patient survival is unclear at present, mainly because these procedures...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198704000-00009
更新日期:1987-04-01 00:00:00
abstract::Spodoptera frugiperda (Sf9) insect cells secreted a class I MHC RT1.Aa heavy chain protein when infected with baculovirus that bore a construct that contained a honeybee melittin secretion (ms) signal attached to RT1.Aa cDNA. The RT1.Aa heavy chain protein in the culture supernatant and cell lysate immunoprecipitated ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199602150-00024
更新日期:1996-02-15 00:00:00
abstract:BACKGROUND:Black American heart transplant recipients receiving cyclosporine-based primary immunoprophylaxis suffer higher rates of allograft rejection with hemodynamic compromise, infections, and posttransplant coronary artery disease. We examined the hypothesis that a combination of tacrolimus and mycophenolate mofet...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-200212150-00014
更新日期:2002-12-15 00:00:00
abstract::Antigenic requirements for the induction of T cell-independent primary splenic IgM antibody responses (plaque-forming cell responses) to H-2Dd alloantigens were studied. Results show that some functional activity or structural property of the donor cells is required for immunogenicity, because antigens are not active ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198302000-00014
更新日期:1983-02-01 00:00:00
abstract::We report two cases of Kaposi's sarcoma (KS) after orthotopic liver transplantation for cirrhosis of the liver related to hepatitis C virus. Both cases were Saudi-born Arabs who were negative for human immunodeficiency virus; one patient was receiving FK506 plus prednisolone, and the other patient was receiving FK506....
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199705270-00025
更新日期:1997-05-27 00:00:00
abstract::The mode of action of glucocorticosteroids as immunosuppressive and antiinflammatory agents is not fully understood. Glucocorticosteroids block synthesis of interleukin 1 by interfering with the transcription of the IL-1 beta gene. Glucocorticosteroids may also induce rapid degradation of IL-1 mRNA. In the presence of...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199001000-00040
更新日期:1990-01-01 00:00:00
abstract::Although extrinsic denervation is inevitable after intestinal transplantation and leads to poor intestinal function, little is known about the occurrence of extrinsic reinnervation. In this study, extrinsic sympathetic reinnervation was investigated morphologically following syngeneic intestinal transplantation perfor...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-02-15 00:00:00
abstract::As a first step in the study of the possible relationship between the T/t and H-2 complexes, the H-2 antigenic composition of the strains carrying factors t12, tw32, tw2, tw8, t1, t0, t6, tw1, tw71, tw73, tw12, tw5, tw75, and t38 was studied by using a battery of antisera containing antibodies against inbred-derived H...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197603000-00004
更新日期:1976-03-01 00:00:00
abstract:BACKGROUND:Cardiovascular disease remains epidemic in transplant recipients, despite aggressive treatment of cardiovascular risk factors. Thus, novel risk factors could play a role in the genesis of cardiovascular events in this population. METHODS:We evaluated the impact of early posttransplant anemia on cardiovascul...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000084872.26360.C5
更新日期:2003-09-15 00:00:00
abstract::We studied the role of interleukin (IL)-8 during engraftment after hematopoietic stem cell transplantation. In 40 consecutive patients undergoing either allogeneic marrow (n=32) or autologous peripheral blood stem cell transplantation (n=8), IL-8 plasma levels were serially determined. Median IL-8 concentrations peake...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000135564.82707.f0
更新日期:2004-10-15 00:00:00
abstract::The new class of immunosuppressants--inhibitors of the mammalian target of rapamycin--has no nephrotoxicity and the capacity to inhibit vascular smooth cell proliferation. These characteristics may afford considerable clinical advantages in the transplantation of kidneys from expanded criteria donors (ECD). Six clinic...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181a058a1
更新日期:2009-04-27 00:00:00