Abstract：
:Interferon beta-1b (IFN beta-1b) (Betaseron) has been recently shown to alter the course of multiple sclerosis (MS), an inflammatory demyelinating disease of the central nervous system (CNS) where migration of activated lymphocytes across the blood-brain barrier (BBB) is a critical step in the pathogenesis of this disease. Magnetic resonance imaging (MRI) studies performed on patients treated with IFN beta-1b showed a remarkable effect on the BBB as determined by a reduction in the number of gadolinium enhancing lesions, a measure of BBB leakiness. Since adhesion molecules (AM) induced on endothelial cells (EC) play an important role in T-cell migration into the CNS, the objective of this study was to examine the effect of IFN beta-1b on the expression of the AM, ICAM-1, V-CAM and E-selection induced on EC by IFN-gamma, TNF-alpha, or IL-1 beta. Primary cultures of human umbilical vein EC (HUVEC) were used, which under basal conditions expressed low levels of AM. IFN beta-1b (1-1000IU/ml) had minimal effect on basal expression of AM on HUVEC, but AM could be substantially upregulated by IFN-gamma, IL-1 beta or TNF-alpha. The effect of IFN beta-1b on AM expression induced by IFN-gamma, IL-1 beta or TNF-alpha was slightly additive. It is concluded that IFN beta-1b does not downregulate the inducible expression of ICAM-1, V-CAM or E-selectin on HUVEC. These findings suggest alternate mechanisms for the effect of IFN beta-1b on the BBB in MS.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Jiang H,Williams GJ,Dhib-Jalbut Sdoi
10.1016/s0197-0186(96)00081-2subject
Has Abstractpub_date
1997-04-01 00:00:00pages
449-53issue
4-5eissn
0197-0186issn
1872-9754journal_volume
30pub_type
杂志文章abstract：:In chicks submitted to light stimulation, the synthesis of gangliosides of the retina ganglion cell increases with respect to chicks maintained in the dark. In an attempt to elucidate if the activation of glycosyltransferases participates in the establishment of these light-dark differences detected in vivo, we examin...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(96)00128-3
更新日期：1997-07-01 00:00:00
abstract：:Transport activity of d-pipecolic acid and of l-pipecolic acid in mouse brain and peripheral tissues were tested, and the effect of immobilization stress was described, along with the method for preparative, enantiomeric resolution and purification of d,l-pipecolic acid using high performance liquid chromatography equ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(88)90057-5
更新日期：1988-01-01 00:00:00
abstract：:Primary cortical neurons exposed to the mitochondrial toxin NaN3 (0.1-3 mM) were submitted to oxidative stress with H2O2 (30-150 μM), to mimic conditions observed in neurodegenerative disorders. The effects of such treatment on a series of parameters useful in characterizing neuronal damage were investigated: (i) the ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2013.05.005
更新日期：2013-08-01 00:00:00
abstract：:The present study confirmed our previous assumption on the crucial role of central alpha2B-like adrenoceptor subtype in gastric mucosal defense. It was found that beside clonidine, rilmenidine, an alpha2/imidazoline receptor agonist and ST-91, an alpha2B-adrenoceptor preferring agonist inhibited the mucosal lesions in...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2007.07.001
更新日期：2007-10-01 00:00:00
abstract：:Tissue preparations from the brain of rats and guinea-pigs were examined for their activity in binding isotopically labelled colchicine, as a measure of their content of tubulin. The amount of binding material extracted into a cold buffered solution was unaffected by keeping tissues at 0 degrees C. It diminished by on...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(83)90072-4
更新日期：1983-01-01 00:00:00
abstract：:Local excitation of the hippocampus with N- methyl- d,l -aspartic acid caused a rapid release of specific proteins into the extracellular fluid. The proteins were recovered from the effluent of a small implanted tubing, permeable to macromolecules, and analyzed by sodium dodecyl sulphate electrophoresis. ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(86)90031-8
更新日期：1986-01-01 00:00:00
abstract：:A pre-requisite to understanding the physiological mechanisms of action of melatonin is the identification of the target sites where the hormone acts. The radioligand 2-[125I]iodo-melatonin has been used extensively to localize binding sites in both the brain and peripheral tissues. In general these binding sites have...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/0197-0186(94)90100-7
更新日期：1994-02-01 00:00:00
abstract：:Long-term (12 h) incubation of NCB-20 neuroblastoma x Chinese hamster brain cell hybrid cells with phorbol dibutylate caused a decrease in the enzyme activity of protein kinase C (PKC). Under these conditions, the formation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] and the mobilization of intracellular Ca(2+) t...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(91)90175-d
更新日期：1991-01-01 00:00:00
abstract：:The effects of the GABA agonist, muscimol on [(3)H]flunitrazepam binding were examined in cerebellum and hippocampus regions proposed to contain different populations of benzodiazepine binding site subtypes. Quantitative analysis was made of the contribution of different components of [(3)H]flunitrazepam binding by ut...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(84)90082-2
更新日期：1984-01-01 00:00:00
abstract：:AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) excitotoxicity was examined in cultured neocortical neurons using the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to measure cell viability. Neurons were exposed to AMPA at different culture periods during development o...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(97)00130-7
更新日期：1998-05-01 00:00:00
abstract：:Age, sex, and gonadal hormones have profound effects on ischemic stroke outcomes, although how these factors impact basic stroke pathophysiology remains unclear. There is a plethora of inconsistent data reported throughout the literature, primarily due to differences in the species examined, the timing and methods use...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2012.10.003
更新日期：2012-12-01 00:00:00
abstract：:The activities of the enzymes glutathione reductase (GRD), glutathione peroxidase (GPX), and glutathione S-transferase (GST) were studied in several rat brain areas following the aspirative transection of the septohippocampal pathway (fimbria fornix) and the administration of nerve growth factor (NGF) or cytochrome c....
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(98)00076-x
更新日期：1999-02-01 00:00:00
abstract：:Gliomas are the most common and malignant primary brain tumours and are associated with a poor prognosis despite the availability of multiple therapeutic options. Quercetin, a traditional Chinese medicinal herb, is an important flavonoid and has anti-cancer activity. Here, we evaluated whether quercetin could inhibit ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2014.12.001
更新日期：2015-01-01 00:00:00
abstract：:It has been shown recently that the N-terminal domain of the dopamine transporter (DAT) plays a role in several transporter functions. Here we provide evidence for a possible cellular mechanism of how the N-terminus of dopamine transporter might be removed in vivo. We isolated a recombinant N-terminal protein region o...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2008.04.001
更新日期：2008-06-01 00:00:00
abstract：:Glutamine has multiple physiological and pathophysiological roles in the brain. Because of their position at the interface between blood and brain, the cerebral capillaries and the choroid plexuses that form the blood-brain barriers (BBB) and blood-cerebrospinal fluid (CSF) barriers, have the potential to influence br...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(03)00013-5
更新日期：2003-09-01 00:00:00
abstract：:Glycine can be substrate for two transporters: GlyT1, largely expressed by astrocytes but also by some non-glycinergic neurons, and GlyT2, most frequently present in glycine-storing nerve endings. In morphological studies, GlyT2 expression had been found to be restricted to caudal regions, being almost undetectable in...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2016.07.001
更新日期：2016-10-01 00:00:00
abstract：:The effects of the peroxovanadium complex potassium bisperoxo(1,10-phenanthroline)-oxovanadate (bpV[phen]) have been studied on dopamine (DA) exocytosis in PC12 cells. Bisperoxo(1,10-phenanthroline)-oxovanadate does not elicit dopamine secretion in PC12 cells. However, treatment of PC12 cells with 30 microM bpV[phen] ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(01)00093-6
更新日期：2002-04-01 00:00:00
abstract：:Acetylcholinesterase (AChE) hydrolyses acetylcholine to choline and acetate, playing an important role in terminating the neurotransmission in brain and muscle. Recently, the non-neuronal functions of AChE have been proposed in different tissues, in which there are various factors to regulate the expression of AChE. I...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2020.104861
更新日期：2020-12-01 00:00:00
abstract：:With their potent regenerative and protective capacities, stem cell-derived conditioned media emerged as an effective alternative to cell therapy, and have a prospect to be manufactured as pharmaceutical products for tissue regeneration applications. Our study investigates the neuroregenerative potential of human dent...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2021.104961
更新日期：2021-01-16 00:00:00
abstract：:In order to elucidate the effects of MPTP on enkephalinergic neurons, dopamine (DA), norepinephrine (NE), proenkephalin (PE) mRNA and met-enkephalin (ME) were measured in striatum, olfactory tubercle, and prefrontal cortex of C57/B16 mice 1 day-2 weeks following treatment with 96 mg/kg MPTP HCl (24 mg/kg i.p., twice/d...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(93)90010-3
更新日期：1993-02-01 00:00:00
abstract：:Microglial activation has been implicated in neurodegenerative diseases. Therefore, inhibition of inflammation mediated by microglia is a strategy in neurodegenerative disease therapy. In this study, we isolated cryptotanshinone and 15,16-dihydrotanshinone I from Salvia miltiorrhiza, a traditional Korean herb medicine...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2005.07.004
更新日期：2006-01-01 00:00:00
abstract：:In recent years there has been a great deal of research within the stem cell field which has led to the definition and classification of a range of stem cells from a plethora of tissues and organs. Stem cells, by classification, are considered to be pluri- or multipotent and have both self-renewal and multi-differenti...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2016.08.001
更新日期：2017-06-01 00:00:00
abstract：:Nerve terminals from the head ganglia of Locusta migratoria were isolated by means of a modified microscale flotation technique. Enzymatic, ultrastructural and chemical analysis revealed that the synaptosomal fraction was highly enriched in well-preserved nerve endings containing almost no free mitochondria. Cholinerg...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(81)90035-8
更新日期：1981-01-01 00:00:00
abstract：:There is a growing body of evidence on the role of nitric oxide (NO) in retinal physiology. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO(-)), because it is formed by a number of diverse biochemical reactions. The aim of the present report was to co...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2007.04.015
更新日期：2007-11-01 00:00:00
abstract：:Differentiating granule cells develop survival requirements in vitro which can be met by treatment with high K+ or excitatory amino acids. Promotion of cell survival by N-methyl-D-aspartate (NMDA) or kainate has already been established and here we report that treatment of the cells with alpha-amino-3-hydroxy-5-methyl...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(94)90067-1
更新日期：1994-09-01 00:00:00
abstract：:The opening of pannexin-1 (Px1) hemichannels is regulated by the activity of P2X(7) receptors (P2X(7)Rs). At present, however, little is known about how extracellular ATP-sensitive P2X(7)Rs regulates the opening and closure of Px1 hemichannels. Several lines of evidence suggest that P2X(7)Rs are activated under pathol...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2010.12.013
更新日期：2011-02-01 00:00:00
abstract：:Chronic ingestion of caffeine causes dependence and sleep disturbance in children and adolescents. In rodents, the administration of caffeine may produce behavioral cross-sensitization to some psychostimulants, such as dopaminergic psychoactive drugs. Methylphenidate (MPH; Ritalin) is a psychostimulant used in pediatr...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2009.03.015
更新日期：2009-09-01 00:00:00
abstract：:Human glutamate dehydrogenase (GDH) gene was fused with a gene fragment encoding the nine amino acid (RKKRRQRRR) protein transduction domain of human immunodeficiency virus TAT protein in bacterial expression vector to produce genetic in-frame TAT-GDH fusion protein. The TAT-GDH protein can enter PC12 cells efficientl...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(01)00138-3
更新日期：2002-07-01 00:00:00
abstract：:Several studies have indicated a functional differentiation across the septotemporal axis of rat hippocampus. Our previous results have shown that the alpha 1 beta 2 gamma 2-GABAA receptor subtype dominates in dorsal hippocampus (DH), while the alpha 2 beta 1 gamma 2-subtype prevails in ventral hippocampus (VH). We th...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2007.10.016
更新日期：2008-05-01 00:00:00
abstract：:Specific binding of [(3)H]d- fenfluramine , an anorectic agent acting on serotonergic system, was characterized in rat total brain minus cerebellum. The binding was reversible, stereospecific and with high affinity (K(d) about 80 nM). The receptor density in total brain was about 32 pmol/g tissue. Subcellular distribu...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(88)90007-1
更新日期：1988-01-01 00:00:00
