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Identification of secreted factors in dental pulp cell-conditioned medium optimized for neuronal growth.

Abstract：

:With their potent regenerative and protective capacities, stem cell-derived conditioned media emerged as an effective alternative to cell therapy, and have a prospect to be manufactured as pharmaceutical products for tissue regeneration applications. Our study investigates the neuroregenerative potential of human dental pulp cells (DPCs) conditioned medium (CM) and defines an optimization strategy of DPC-CM for enhanced neuronal outgrowth. Primary sensory neurons from mouse dorsal root ganglia were cultured with or without DPC-CM, and the lengths of βIII-tubulin positive neurites were measured. The impacts of several manufacturing features as the duration of cell conditioning, CM storage, and preconditioning of DPCs with some factors on CM functional activity were assessed on neurite length. We observed that DPC-CM significantly enhanced neurites outgrowth of sensory neurons in a concentration-dependent manner. The frozen storage of DPC-CM had no impact on experimental outcomes and 48 h of DPC conditioning is optimal for an effective activity of CM. To further understand the regenerative feature of DPC-CM, we studied DPC secretome by human growth factor antibody array analysis and revealed the presence of several factors involved in either neurogenesis, neuroprotection, angiogenesis, and osteogenesis. The conditioning of DPCs with the B-27 supplement enhanced significantly the neuroregenerative effect of their secretome by changing its composition in growth factors. Here, we show that DPC-CM significantly stimulate neurite outgrowth in primary sensory neurons. Moreover, we identified secreted protein candidates that can potentially promote this promising regenerative feature of DPC-CM.

journal_name

Neurochem Int

journal_title

Neurochemistry international

authors

Chouaib B,Collart-Dutilleul PY,Blanc-Sylvestre N,Younes R,Gergely C,Raoul C,Scamps F,Cuisinier F,Romieu O

doi

10.1016/j.neuint.2021.104961

subject

Has Abstract

pub_date

2021-01-16 00:00:00

pages

104961

eissn

0197-0186

issn

1872-9754

pii

S0197-0186(21)00007-3

journal_volume

144

pub_type

杂志文章

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