Functional significance of the negative-feedback regulation of ATP release via pannexin-1 hemichannels under ischemic stress in astrocytes.

Abstract:

:The opening of pannexin-1 (Px1) hemichannels is regulated by the activity of P2X(7) receptors (P2X(7)Rs). At present, however, little is known about how extracellular ATP-sensitive P2X(7)Rs regulates the opening and closure of Px1 hemichannels. Several lines of evidence suggest that P2X(7)Rs are activated under pathological conditions such as ischemia, resulting in the opening of Px1 hemichannels responsible for the massive influx of Ca(2+) from the extracellular space and the release of ATP from the cytoplasm, leading to cell death. Here we show in cultured astrocytes that the suppression of the activity of P2X(7)Rs during simulated ischemia (oxygen/glucose deprivation, OGD) resulted in the opening of Px1 hemichannels, leading to the enhanced release of ATP. In addition, the suppression of the activity of P2X(7)Rs during OGD resulted in a significant increase in astrocytic damage. Both the P2X(7)Rs suppression-induced enhancement of the release of ATP and cell damage were reversed by co-treatment with blockers of Px1 hemichannels, suggesting that suppression of the activity of PX(7)Rs resulted in the opening of Px1 hemichannels. All these findings suggested the existence of a negative-feedback loop regulating the release of ATP via Px1 hemichannels; ATP-induced suppression of ATP release. The present study indicates that ATP, released through Px1 hemichannels, activates P2X(7)Rs, resulting in the closure of Px1 hemichannels during ischemia. This negative-feedback mechanism, suppressing the loss of cellular ATP and Ca(2+) influx, might contribute to the survival of astrocytes under ischemic stress.

journal_name

Neurochem Int

authors

Iwabuchi S,Kawahara K

doi

10.1016/j.neuint.2010.12.013

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

376-84

issue

3

eissn

0197-0186

issn

1872-9754

pii

S0197-0186(10)00383-9

journal_volume

58

pub_type

杂志文章
  • Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice.

    abstract::Several glycoproteins in mammalian brains contain α2,8-linked disialic acid residues. We previously showed a constant expression of disialic acid (DiSia) in the hippocampus, olfactory bulb and cortex, and a gradual decrease of expression in the cerebellum from neonatal to senile mice. Previous publications indicate th...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2013.07.013

    authors: Rinflerch AR,Burgos VL,Ielpi M,Quintana MO,Hidalgo AM,Loresi M,Argibay PF

    更新日期:2013-11-01 00:00:00

  • Unchanged glutamine synthetase activity and increased NMDA receptor density in epileptic human neocortex: implications for the pathophysiology of epilepsy.

    abstract::We investigated whether alterations in glutamate metabolising glutamine synthetase activity occur in human epileptic neocortex, as shown previously for human epileptic hippocampus [Eid, T., Thomas, M.J., Spencer, D.D., Rundén-Pran, E., Lai, J.C.K., Malthankar, G.V., Kim, J.H., Danbolt, N.C., Ottersen, O.P., de Lanerol...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2005.06.001

    authors: Steffens M,Huppertz HJ,Zentner J,Chauzit E,Feuerstein TJ

    更新日期:2005-11-01 00:00:00

  • Expression of lysosomal protective protein/cathepsin A in a stably transformed human neuroblastoma cell line during bi-directional differentiation into neuronal and Schwannian cells.

    abstract::Human neuroblastoma GOTO cell lines were established that stably express recombinant human lysosomal protective protein/cathepsin A (PPCA) cDNA by transfection. Intracellular cathepsin A (acid serine carboxypeptidase) activity increased four-fold compared with in those of the parent and mock-transfected cell lines. Th...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2003.07.001

    authors: Itoh K,Satoh Y,Kadota Y,Oheda Y,Kuwahara J,Shimmoto M,Sakuraba H

    更新日期:2004-05-01 00:00:00

  • Acidosis and astrocyte amino acid metabolism.

    abstract::The relationship between acidosis and the metabolism of glutamine and glutamate was studied in cultured astrocytes. Acidification of the incubation medium was associated with an increased formation of aspartate from glutamate and glutamine. The rise of the intracellular content of aspartate was accompanied by a signif...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(99)00141-2

    authors: Yudkoff M,Daikhin Y,Nissim I,Nissim I

    更新日期:2000-04-01 00:00:00

  • Hypothalamic GABA-ergic activity and T-cell proliferation in aged mammal: effect of dietary protein.

    abstract::Short-term supplementation of low protein diet (LPD) or high protein diet (HPD) to the aged (18 months old) rats did not change the hypothalamic GABAergic activity and immune response. Long-term supplementation of LPD on the other hand, enhanced hypothalamic GABAergic activity with an immunopotentiation by increasing ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(97)00062-4

    authors: Bandyopadhyay BC,Poddar MK

    更新日期:1998-02-01 00:00:00

  • Diet-induced ketosis increases monocarboxylate transporter (MCT1) levels in rat brain.

    abstract::Monocarboxylate transporter (MCT1) levels in brains of adult Long-Evans rats on a high-fat (ketogenic) diet were investigated using light and electron microscopic immunocytochemical methods. Rats given the ketogenic diet (91% fat and 9% protein) for up to 6 weeks had increased levels of the monocarboxylate transporter...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(00)00102-9

    authors: Leino RL,Gerhart DZ,Duelli R,Enerson BE,Drewes LR

    更新日期:2001-05-01 00:00:00

  • N-acetyl cysteine ameliorates depression-induced cognitive deficits by restoring the volumes of hippocampal subfields and associated neurochemical changes.

    abstract::Depression is highly comorbid with anxiety disorders and associated with profound cognitive impairment. Moreover, cognitive deficits associated with hippocampal dysfunction are central in depression and anxiety disorders. Furthermore, depression is accompanied by glutamatergic dysfunction which can further impair the ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2019.104605

    authors: Chakraborty S,Tripathi SJ,Srikumar BN,Raju TR,Shankaranarayana Rao BS

    更新日期:2020-01-01 00:00:00

  • Expression of growth inhibitory factor (GIF) in normal and injured rat brains.

    abstract::Immunohistochemical study on growth inhibitory factor (GIF) in rat brain has revealed that a glial cell layer on the surface of cerebral cortex and the cells surrounding Purkinje cells has been reported. In addition, neurons in gray matter were weakly immunostained for GIF. In situ hybridization using digoxigenin-labe...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(94)00170-y

    authors: Anezaki T,Ishiguro H,Hozumi I,Inuzuka T,Hiraiwa M,Kobayashi H,Yuguchi T,Wanaka A,Uda Y,Miyatake T

    更新日期:1995-07-01 00:00:00

  • R659S mutation of gammaPKC is susceptible to cell death: implication of this mutation/polymorphism in the pathogenesis of retinitis pigmentosa.

    abstract::It has been reported that mutations of gammaPKC cause hereditary spinocerebellar atrophy type 14 (SCA14). Our recent study has revealed that the SCA14 mutant gammaPKC is susceptible to aggregation and causes cell death. Among mutations/polymorphisms of gammaPKC, the R659S mutation was firstly segregated from families ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2006.05.005

    authors: Mochizuki H,Seki T,Adachi N,Saito N,Mishima HK,Sakai N

    更新日期:2006-12-01 00:00:00

  • Effects of lithium, alone or associated with pilocarpine, on muscarinic and dopaminergic receptors and on phosphoinositide metabolism in rat hippocampus and striatum.

    abstract::The mechanism of action of lithium (Li) alone or with pilocarpine (Pilo), focusing on muscarinic and dopaminergic systems and also on phosphoinositide metabolism was studied. Li (3 mEq/kg) administered to rats once (1 d) or daily for 7 days (7 d), 24 h before Pilo (15 mg/kg), exacerbated cholinergic signs, leading to ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(98)00028-x

    authors: Marinho MM,de Sousa FC,de Bruin VM,Vale MR,Viana GS

    更新日期:1998-10-01 00:00:00

  • Opposite effects of cholecystokinin octapeptide (CCK-8) and tetrapeptide (CCK-4) after injection into the caudal part of the nucleus accumbens or into its rostral part and the cerebral ventricles.

    abstract::Neurons with colocalized cholecystokinin and dopamine are present predominantly in the ventral tegmental area and project mainly to the caudal part of the medial nucleus accumbens. The activity of this dopamine system can be evaluated by means of the intracranial self-stimulation behavior on male Wistar rats having ch...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(87)90074-x

    authors: De Witte P,Heidbreder C,Roques B,Vanderhaeghen JJ

    更新日期:1987-01-01 00:00:00

  • Vardenafil: structural basis for higher potency over sildenafil in inhibiting cGMP-specific phosphodiesterase-5 (PDE5).

    abstract::Phosphodiesterase-5 (PDE5) inhibitors act by competing with the substrate, cGMP, for the catalytic site of the enzyme. Two commercialized PDE5 inhibitors, sildenafil and vardenafil, are being used to treat erectile dysfunction. These two compounds differ in the heterocyclic ring system used to mimic the purine ring of...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2004.03.016

    authors: Corbin JD,Beasley A,Blount MA,Francis SH

    更新日期:2004-11-01 00:00:00

  • Secreted gingipains from Porphyromonas gingivalis induce microglia migration through endosomal signaling by protease-activated receptor 2.

    abstract::Much attention has been paid to the connection between periodontitis and Alzheimer's disease (AD). We previously showed that infection of P. gingivalis, one of major periodontal pathogen causing periodontitis, induced migration and inflammatory responses in murine microglia through gingipain-induced activation of prot...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2020.104840

    authors: Nonaka S,Nakanishi H

    更新日期:2020-11-01 00:00:00

  • Inhibition of chick brain cholesterogenic enzymes by phenyl and phenolic derivatives of phenylalanine.

    abstract::Phenylalanine and its phenyl metabolites produced a clear inhibition of chick brain mevalonate 5-pyrophosphate decarboxylase, while mevalonate kinase and mevalonate 5-phosphate kinase were not significantly affected. Phenolic derivatives produced a similar or higher inhibition than that found in the presence of phenyl...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(91)90182-d

    authors: Castillo M,Iglesias J,Zafra MF,Garcia-Peregrin E

    更新日期:1991-01-01 00:00:00

  • Metabolism of [1-(13)C)glucose and [2-(13)C]acetate in the hypoxic rat brain..

    abstract::The effects of hypoxia on the metabolism of the central nervous system were investigated in rats submitted to a low oxygen atmosphere (8% O(2); 92% N(2)). [1-(13)C]glucose and [2-(13)C]acetate were used as substrates, this latter being preferentially metabolized by glial cells. After 1-h substrate infusion, the incorp...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(00)00106-6

    authors: Chateil J,Biran M,Thiaudière E,Canioni P,Merle M

    更新日期:2001-04-01 00:00:00

  • Characterization of guanylate cyclase in frog retina using nitrosoguanidine and superoxide dismutase.

    abstract::Guanylate cyclase in the membrane fraction of rod outer segments (ROS-membrane) from the frog retina showed an extremely high activity but had no responsiveness to NaN(3) (+catalase), nitrosoguanidine or superoxide dismutase. These compounds, however, markedly activated the enzyme in a soluble fraction from the retina...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(82)90006-7

    authors: Yoshikawa K,Nishimura C,Kuriyama K

    更新日期:1982-01-01 00:00:00

  • Autism genes are selectively targeted by environmental pollutants including pesticides, heavy metals, bisphenol A, phthalates and many others in food, cosmetics or household products.

    abstract::The increasing incidence of autism suggests a major environmental influence. Epidemiology has implicated many candidates and genetics many susceptibility genes. Gene/environment interactions in autism were analysed using 206 autism susceptibility genes (ASG's) from the Autworks database to interrogate ∼1 million chemi...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuint.2016.10.011

    authors: Carter CJ,Blizard RA

    更新日期:2016-10-27 00:00:00

  • Understanding the epigenetic landscape and cellular architecture of childhood brain tumors.

    abstract::Pediatric brain tumors are the leading cancer-related cause of death in children and adolescents in the United States, affecting on average 1 in 2000 children per year. Recent advances in cancer genomics have led to profound discoveries about the underlying molecular biology and ontogeny of these tumors. In particular...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2020.104940

    authors: Alencastro Veiga Cruzeiro G,Rota C,Hack OA,Segal R,Filbin MG

    更新日期:2020-12-15 00:00:00

  • The unresolved role of mitochondrial DNA in Parkinson's disease: An overview of published studies, their limitations, and future prospects.

    abstract::Parkinson's disease (PD), a progressive neurodegenerative disorder, has long been associated with mitochondrial dysfunction in both sporadic and familial forms of the disease. Mitochondria are crucial for maintaining cellular homeostasis, and their dysfunction is detrimental to dopaminergic neurons. These neurons are ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuint.2019.104495

    authors: Müller-Nedebock AC,Brennan RR,Venter M,Pienaar IS,van der Westhuizen FH,Elson JL,Ross OA,Bardien S

    更新日期:2019-10-01 00:00:00

  • Substrate-induced up-regulation of Na(+)-dependent glutamate transport activity.

    abstract::Sodium-dependent transporters regulate extracellular glutamate in the CNS. Recent studies suggest that the activity of several different neurotransmitter transporters can be rapidly regulated by a variety of mechanisms. In the present study, we report that pre-incubation of primary 'astrocyte-poor' neuronal cultures w...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(00)00018-8

    authors: Munir M,Correale DM,Robinson MB

    更新日期:2000-08-01 00:00:00

  • Distinct modulation of a gene expression of the type 1 and 2 IP(3) receptors by retinoic acid in brain areas.

    abstract::Inositol 1,4,5-trisphosphate (IP(3)) receptors belong to the intracellular calcium channels that release calcium from the intracellular stores after binding IP(3). Three types of IP(3) receptors occurred in a tissue specific manner and different promoters direct their gene expression. Thus, understanding of the transc...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2005.01.003

    authors: Stefanik P,Macejova D,Mravec B,Brtko J,Krizanova O

    更新日期:2005-06-01 00:00:00

  • Modification of GABA and calcium uptake by lipids in synaptosomes from normoxic and ischemic brain.

    abstract::The effects of membrane lipid disturbances induced by ischemia and exogenously added lipids on the uptake of GABA and Ca(2+) were investigated in gerbil brain synaptosomes. Ischemia was produced by bilateral ligation of common carotid arteries in Mongolian gerbil for 10 min. The level of the free fatty acids (FFA) inc...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(86)90101-4

    authors: Noremberg K,Strosznajder J

    更新日期:1986-01-01 00:00:00

  • Abnormal glycosylation of myelin-associated glycoprotein in quaking mouse brain.

    abstract::Brain slices from actively myelinating (26-28 days) quaking and normal littermates were dual-labeled with radioactive mannose and fucose for 2 h. Following the incubation myelin was isolated by sucrose density gradient centrifugation and the incorporation of sugars into the major myelinassociated glycoprotein (MAG) de...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(87)90084-2

    authors: Konat G,Hogan EL,Leskawa KC,Gantt G,Singh I

    更新日期:1987-01-01 00:00:00

  • Immunohistoblot analysis on whole human hemispheres from normal and Alzheimer diseased brains.

    abstract::We demonstrate the feasibility and usefulness of the histoblot immunostaining of cryosections of whole hemispheres of healthy and Alzheimer diseased (AD) human brains by localizing a neuron-specific marker, the anti-neuronal nuclei (NeuN) antigen. As expected, cortical NeuN-immunopositive regions were generally thinne...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2008.09.002

    authors: Beliczai Z,Varszegi S,Gulyas B,Halldin C,Kasa P,Gulya K

    更新日期:2008-12-01 00:00:00

  • Cross-inhibition of angiotensin AT1 receptors supports the concept of receptor oligomerization.

    abstract::G protein-coupled receptors are cell surface receptors that mediate the effects of extracellular signals in the endocrine/paracrine and sensory systems. Experimental evidence is accumulating, which suggest that these receptors form dimers or higher order oligomers. The functional relevance of G protein-coupled recepto...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2007.05.018

    authors: Karip E,Turu G,Süpeki K,Szidonya L,Hunyady L

    更新日期:2007-10-01 00:00:00

  • Electrophoretic pattern and distribution of cytoskeletal proteins in flat-epitheloid and stellate process-bearing astrocytes in primary culture.

    abstract::One- and two-dimensional electrophoresis patterns and distribution of major cytoskeletal proteins were studied in primary astrocytes with either flat-epitheloid or stellate appearance. No major differences in the electrophoretic patterns of actin, tubulin, glial fibrillary acidic protein (GFAP) and vimentin were detec...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(84)90126-8

    authors: Ciesielski-Treska J,Ulrich G,Mensch C,Aunis D

    更新日期:1984-01-01 00:00:00

  • Regulation of ganglioside and sialoglycoprotein biosynthesis-Effect of drugs affecting membrane flow.

    abstract::The labelling of gangliosides and sialoglycoproteins (SGP) after injecting intraocularly 30 ?Ci of [(3)H]ManNAc was studied in chickens that had previously received, also intraocularly, 30 or 70 nmol of monensin in 10 ?l of ethanol or 24 ?g of colchicine in 10 ?l saline. Controls received ethanol or saline only. Colch...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(86)90183-x

    authors: Nores GA,Caputto R

    更新日期:1986-01-01 00:00:00

  • Agonist binding fraction of dopamine D2/3 receptors in rat brain: a quantitative autoradiographic study.

    abstract::There has arisen considerable interest in the study of dopamine D(2/3) agonist binding sites by positron emission tomography (PET), based on the claim that agonist sites represent a functional subset of the total number of sites labeled by more conventional antagonist ligands. To test the basis of this claim, we used ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2010.01.010

    authors: Minuzzi L,Cumming P

    更新日期:2010-05-01 00:00:00

  • CNS distribution of D1 receptors: use of a new specific D1 receptor antagonist, [3H]SCH39166.

    abstract::D1 dopamine receptors have been localized using a radioactive form of a new specific antagonist, [3H]SCH39166. This compound has been shown, in in vitro binding studies, to be highly selective for the D1 receptor subtype; more so than its predecessor, [3H]SCH23390. These ligand binds saturably, reversibly and with hig...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审

    doi:10.1016/0197-0186(92)90224-f

    authors: Wamsley JK,Alburges ME,McQuade RD,Hunt M

    更新日期:1992-03-01 00:00:00

  • Adenosine and dopamine receptor antagonist binding in the rat ventral and dorsal striatum: lack of changes after a neonatal bilateral lesion of the ventral hippocampus.

    abstract::There is experimental evidence from radioligand binding experiments for the existence of strong antagonistic interactions between different subtypes of adenosine and dopamine receptors in the striatum, mainly between adenosine A1 and dopamine D1 and between adenosine A2A and dopamine D2 receptors. These interactions s...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(99)00008-x

    authors: Lillrank SM,Lipska BK,Weinberger DR,Fredholm BB,Fuxe K,Ferré S

    更新日期:1999-03-01 00:00:00