Abstract：
:In chicks submitted to light stimulation, the synthesis of gangliosides of the retina ganglion cell increases with respect to chicks maintained in the dark. In an attempt to elucidate if the activation of glycosyltransferases participates in the establishment of these light-dark differences detected in vivo, we examined the activity of a key ganglioside glycosyltransferase, the GalNAc-T (N-acetylgalactosaminyltransferase) that converts GM3 to GM2, in the retina ganglion cells isolated from light and dark exposed chicks. We found that GalNAc-T and other glycosyltransferases are active in these ganglion cell preparations; the kinetic parameters for GalNAc-T were similar to those previously reported for chick retina. The other glycosyltransferase activities assayed were the galactosyltransferase (Gal-T2) that converts GM2 to GM1 and the N-acetylneuraminyltransferase (Sialyl-T1) that converts lactosylceramide to GM3. The three glycosyltransferase activities were higher in the ganglion cell preparations obtained from chicks exposed to light compared to those maintained in the dark. For the GalNAc-T activity, the differences disappear when the cell preparations are sonicated or if the assays are carried out in the presence of detergents or if the end product of the reaction is added to the incubates. The results indicate that the activation of the glycosyltransferases is part of the phenomenon required for cells to achieve the precise rate of synthesis of gangliosides needed in vivo.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Bussolino DF,Guido ME,Caputto BLdoi
10.1016/s0197-0186(96)00128-3subject
Has Abstractpub_date
1997-07-01 00:00:00pages
105-11issue
1eissn
0197-0186issn
1872-9754pii
S0197-0186(96)00128-3journal_volume
31pub_type
杂志文章abstract：:The rat retinal ganglion cell (RGC) line RGC-5 constitutes a widely used model for studying physiological processes in retinal cells. In this paper we investigated the expression of clock and immediately early genes, and calcium mediated responses to physiological stimuli in differentiated and mitotically active RGC-5...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2010.05.013
更新日期：2010-10-01 00:00:00
abstract：:Peroxisome proliferator-activated receptors (PPARα, -β/δ and -γ) are lipid-activated transcription factors. Synthetic PPARα and PPARγ ligands have neuroprotective properties. Recently, PPARβ/δ activation emerged as the focus of a novel approach for the treatment of a wide range of neurodegenerative diseases. To fill t...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2013.06.012
更新日期：2013-10-01 00:00:00
abstract：:The ketogenic diet (KD) is used for the treatment of refractory epilepsy in children, however, the mechanism(s) remains largely unknown. Also, the antiepileptogenic potential in animal models of epilepsy has been poorly addressed. Activation of cannabinoid type-1 receptor (CB(1)-R) upon seizure activity may mediate ne...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2008.10.012
更新日期：2009-03-01 00:00:00
abstract：:Many studies indicate that chronic stress and excessive stress hormone can cause an inflammatory response. Thioredoxin-interacting protein (Txnip) as an endogenous thioredoxin inhibitor suppresses thioredoxin-produced antioxidant effects. Txnip was also found to interact with nucleotide-binding oligomerization domain-...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2019.104564
更新日期：2019-12-01 00:00:00
abstract：:We showed that dextromethorphan (DM) provides neuroprotective/anticonvulsant effects and that DM and its major metabolite, dextrorphan, have a high-affinity for sigma(1) receptors, but a low affinity for sigma(2) receptors. In addition, we found that DM has a higher affinity than DX for sigma(1) sites, whereas DX has ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2007.01.008
更新日期：2007-05-01 00:00:00
abstract：:In the present study we have investigated the relationship between serotonergic receptor(s) (5-HTR) and muscarinic cholinergic receptor (mAChR) activation, leading to the arachidonic acid (AA) release and inositol phosphates (IPs) formation in adult and aged brain cortex synaptoneurosomes. It was observed that seroton...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(95)00001-o
更新日期：1995-06-01 00:00:00
abstract：:Streptozotocin (STZ) is a glucosamine-nitrosourea commonly used to induce long-lasting models of diabetes mellitus and Alzheimer's disease. Direct toxicity of STZ on the pancreas and kidneys has been well characterized, but the acute effect of this compound on brain tissue has received less attention. Herein, we inves...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2019.04.013
更新日期：2019-09-01 00:00:00
abstract：:The distribution of substance P in the colon of piebald lethal (s(1)/s(1)) mice was studied by radioimmunoassay and immunohistochemistry. These animals inherit as a Mendelian recessive trait an aganglionic distal colon. In the region proximal to the aganglionic segment, there is an extensive transitional or hypogangli...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(84)90026-3
更新日期：1984-01-01 00:00:00
abstract：:Acyl-CoA synthetase activity was investigated in membrane fractions from normal and Trembler mouse sciatic nerves. Stearoyl-CoA synthesis in the Trembler assays was always about twice that observed in the control experiments under various conditions of CoA and ATP concentrations. In experiments using varying concentra...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(85)90035-x
更新日期：1985-01-01 00:00:00
abstract：:Patients with Parkinson's disease (PD) show a common progressive neurodegenerative movement disorder characterized by rigidity, tremors, postural instability, and bradykinesia due to the loss of dopaminergic neurons in the substantia nigra, and is often accompanied by several non-motor symptoms, called parkinsonism. S...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2020.104816
更新日期：2020-10-01 00:00:00
abstract：:A sandwich enzyme immunoassay (EIA) was established by using purified human serum dopamine-?-hydroxylase (DBH) as a standard protein and a monospecific polyclonal antibody raised against human DBH purified from human pheochromocytoma. The EIA was applied to measuring DBH levels in human CSF from Parkinsonian patients ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(88)90126-x
更新日期：1988-01-01 00:00:00
abstract：:Two signaling pathways, phosphoinositide 3-kinase (PI-3k)/Akt and Ras/MAPK, are major effectors triggered by nerve growth factor (NGF). Rac1, Cdc42 and GSK-3beta are reported to be targets of PI-3k in the signal transduction for neurite outgrowth. Immediately after NGF was added, broad ruffles were observed temporaril...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2007.04.032
更新日期：2007-07-01 00:00:00
abstract：:This study aimed to elucidate locomotor activity changes in 6-hydroxydopamine (6-OHDA) induced Parkinson's disease (PD) and investigate the possible beneficial effects of melatonin on altered levels of locomotor activity, cyclooxygenase (COX), prostaglandin E2 (PGE2), nuclear factor kappa-B (NF-κB), nitrate/nitrite an...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2014.09.005
更新日期：2014-12-01 00:00:00
abstract：:The iron siderophore binding protein lipocalin 2 (LCN2, also known as 24p3, NGAL and siderocalin) may be involved in iron homeostasis, but to date, little is known about expression of its putative receptor, brain-type organic cation transporter (BOCT, also known as BOCT1, 24p3R, NGALR and LCN2R), in the brain during n...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2015.04.009
更新日期：2015-08-01 00:00:00
abstract：:The effects of dithiothreitol (DTT) and, reduced (GSH) and oxidized (GSSG), glutathione on the release of [3H]GABA evoked by glutamate and its agonists were studied in rat hippocampal slices. DTT had no effect on the basal release of [3H]GABA but it enhanced and prolonged the glutamate agonist-evoked release. This eff...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(94)90010-8
更新日期：1994-06-01 00:00:00
abstract：:The effect of the N-methylisoquinolinium ion (NMIQ(+)) on the activity of enzymes related to metabolism of dopamine was studied using a rat clonal pheochromocytoma PC12h cell line. The activities of tyrosine hydroxylase (TH), aromatic l-amino acid decarboxylase (AADC) and monoamine oxidase (MAO) were inhibited by NMIQ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(89)90138-1
更新日期：1989-01-01 00:00:00
abstract：:Rapid administration of large doses of ammonia leads to death of animals, which is largely prevented by pretreatment with N-methyl-D-aspartate (NMDA) receptor antagonists. The present study focuses on a subunit(s) of NMDA receptor involved in ammonia-induced death by use of NMDA receptor GluRepsilon subunit-deficient ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(03)00124-4
更新日期：2004-01-01 00:00:00
abstract：:A significant loss of dopamine was found in rat striatal slices incubated with 1-methyl-4-phenylpyridinium ion (MPP+) at a concentration of 2 microM or higher. The addition of 7-nitroindazole, a specific inhibitor of neuronal nitric oxide synthase (nNOS), prevented this effect on dopamine when the concentration of MPP...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(05)80006-3
更新日期：1998-07-01 00:00:00
abstract：:The Mg(2+)-ATPase associated with highly purified cholinergic synaptic vesicles isolated from the Torpedo electric organ can be activated by millimolar concentrations of Ca(2+) only to a maximum of 46%. ATP is hydrolyzed in preference to other tri-nucleotides on Mg(2+)-activation but not on Ca(2+)-activation of the en...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(87)90156-2
更新日期：1987-01-01 00:00:00
abstract：:Using a consistent, reproducible and reliable cortical focal ischemia in rat (permanent unilateral occlusion of the left middle cerebral artery & the ipsilateral common carotid artery [MCAo + CCAo] with a 1 h temporary occlusion of the contralateral CCA), the levels of four major membrane fatty acids (palmitic, C16:0;...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(93)90094-l
更新日期：1993-08-01 00:00:00
abstract：:The effect of different dietary fats with varying degrees of unsaturation and essential fatty acid composition, which are commonly consumed in India, on the activity of some important membrane-bound enzymes was assessed in different brain regions of rat. Four groups of male CFY weanling rats were fed nutritionally ade...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(97)00037-5
更新日期：1997-12-01 00:00:00
abstract：:We have previously reported that, unlike their rat counterparts, the survival of mouse cerebellar granule cells is independent of chronic stimulation whether owing to elevated K(+)-induced depolarization or NMDA (N-methyl-D-aspartate) receptor activation. One explanation could be that during the critical period mouse ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(96)00018-6
更新日期：1996-11-01 00:00:00
abstract：:Copper is an essential element that is required for a variety of important cellular functions. Since not only copper deficiency but also excess of copper can seriously affect cellular functions, the cellular copper metabolism is tightly regulated. In brain, astrocytes appear to play a pivotal role in the copper metabo...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2012.08.017
更新日期：2013-04-01 00:00:00
abstract：:The present study has been designed to establish the potential benefits from 1-methylnicotinamide (MNA) treatment on brain disorders associated with type 1 diabetes. All experiments were carried out after 6 weeks of streptozotocin-induced diabetes (60 mg/kg of body weight, i.p.) in male Wistar rats treated for 5 weeks...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2009.10.004
更新日期：2010-01-01 00:00:00
abstract：:Inositol 1,4,5-trisphosphate (IP(3)) receptors belong to the intracellular calcium channels that release calcium from the intracellular stores after binding IP(3). Three types of IP(3) receptors occurred in a tissue specific manner and different promoters direct their gene expression. Thus, understanding of the transc...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2005.01.003
更新日期：2005-06-01 00:00:00
abstract：:Transglutaminase (TGase) activity is increased in affected regions of brains from patients with Huntington's disease (HD). TGase activity is particularly elevated in the nucleus compared with the cytoplasm from these brains. Gamma-glutaminyl-lysyl cross-links have been detected in nuclear inclusions in HD brain, indic...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/s0197-0186(01)00060-2
更新日期：2002-01-01 00:00:00
abstract：:Whereas carbachol, noradrenaline, serotonin and memantine stimulated inositol phosphates production and calcium mobilization in 3-5 day old rabbit retinal cultures, only carbachol and noradrenaline were effective when 25-30 day old cultures were used. The older retinal cultures contain only Müller cells which shows th...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(92)90183-r
更新日期：1992-10-01 00:00:00
abstract：:gamma-Aminobutyric acid (GABA) levels and the activity of glutamate decarboxylase were measured in homogenates of rat brain cortical tissue, at different times after chronic intracortical infusion of GABA in vivo during 2, 6 or 24 h. Cortical electrical activity was also recorded. As previously described, about 1 h af...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(94)90114-7
更新日期：1994-04-01 00:00:00
abstract：:The kinetics of oxidation of endogenous antioxidants such as vitamins C and E and thiols as well as membrane cholesterol in isolated rat brain mitochondria were studied. Oxidation was induced by incubating the mitochondria at 37 degrees C with the free radical generators 2,2' azobis (2'-amidinopropane) dihydrochloride...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(94)00147-m
更新日期：1995-05-01 00:00:00
abstract：:In this study we determined the changes in the intracellular free Ca2+ concentration, associated with the inhibitory modulation of the exocytotic release of GABA by GABAB receptor activation in rat cerebrocortical synaptosomes. We observed that SK&F 97541 and (-)baclofen both act as agonists of the presynaptic GABAB r...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(95)00021-y
更新日期：1995-10-01 00:00:00
