Abstract:
:The relationship between mammalian facilitative glucose transport proteins (GLUT) and multidrug resistance was examined in two vincristine (VCR)-selected murine erythroleukaemia (MEL) PC4 cell lines. GLUT proteins, GLUT1 and GLUT3, were constitutively coexpressed in the parental cell line and also in the VCR-selected cell lines. Increased expression of the GLUT1 isoform was noted both in the PC-V40 (a non-P-glycoprotein, mrp-overexpressing subline) and in the more resistant PC-V160 (overexpressing mrp and mdr3) cell lines. Overexpression of GLUT3 was detected only in the PC-V160 subline. An increased rate of facilitative glucose transport (Vmax) and level of plasma membrane GLUT protein expression paralleled increased VCR resistance, active VCR efflux and decreased VCR steady-state accumulation in these cell lines. Glucose transport inhibitors (GTIs), cytochalasin B (CB) and phloretin blocked the active efflux and decreased steady-state accumulation of VCR in the PC-V40 subline. GTIs did not significantly affect VCR accumulation in the parental or PC-V160 cells. A comparison of protein sequences among GLUT1, GLUT3 and MRP revealed a putative cytochalasin B binding site in MRP, which displayed 44% sequence similarity/12% identity with that previously identified in GLUT1 and GLUT3; these regions also exhibited a similar hydropathy plot pattern. The findings suggested that CB bound to MRP and directly or indirectly lowered VCR efflux and/or CB bound to one or both GLUT proteins, which acted to lower the VCR efflux mediated by MRP. This is the first report of a non-neuronal murine cell line that expressed GLUT3.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Martell RL,Slapak CA,Levy SBdoi
10.1038/bjc.1997.27subject
Has Abstractpub_date
1997-01-01 00:00:00pages
161-8issue
2eissn
0007-0920issn
1532-1827journal_volume
75pub_type
杂志文章abstract:BACKGROUND:Previous studies showed an increased risk of thyroid cancer among children and adolescents exposed to radioactive iodines released after the Chornobyl (Chernobyl) accident, but the effects of screening, iodine deficiency, age at exposure and other factors on the dose-response are poorly understood. METHODS:...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605967
更新日期:2011-01-04 00:00:00
abstract:BACKGROUND:We used bleomycin, etoposide, cisplatin (BEP), the most effective regimen in the treatment of germ cell tumours (GCTs) and increased dose-density by using pegfilgrastim to shorten cycle length. Our aim was to assess safety and tolerability. METHODS:Sixteen male patients with intermediate or poor prognosis m...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.309
更新日期:2011-09-06 00:00:00
abstract::Gallium-67 is a radionuclide that accumulates in haematological malignancies and is used for diagnostic purposes. Uptake of 67Ga into the cell occurs via the transferrin receptor, which is differentially expressed during the various cell cycle phases. With the aim of selectively increasing 67Ga uptake, we studied whet...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.411
更新日期:1996-08-01 00:00:00
abstract:BACKGROUND:Dichloroacetate (DCA), through the inhibition of aerobic glycolysis (the 'Warburg effect') and promotion of pyruvate oxidation, induces growth reduction in many tumours and is now undergoing several clinical trials. If aerobic glycolysis is active in multiple myeloma (MM) cells, it can be potentially targete...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.120
更新日期:2013-04-30 00:00:00
abstract::This study evaluated the impact of province-wide treatment guidelines on consistency of adjuvant therapy for node-negative breast cancer. A retrospective population-based cohort study was conducted in the Canadian provinces of British Columbia, which has province-wide guidelines, and Ontario, which does not. All eligi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.262
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Systemic failure remains a challenge in rectal cancer. We investigated the possible systemic anti-tumour immune activity invoked within oxaliplatin-based neoadjuvant therapy. METHODS:In two high-risk patient cohorts, we assessed the circulating levels of the fms-like tyrosine kinase 3 ligand (Flt3L), a fact...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-018-0085-y
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:We studied the biological significance of genes involved in a novel t(8;12)(p21.3;p13.31) reciprocal translocation identified in cervical squamous cell carcinoma (SCC) cells. METHODS:The rearranged genes were identified by breakpoint mapping, long-range PCR and sequencing. We investigated gene expression in...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.301
更新日期:2012-08-07 00:00:00
abstract:BACKGROUND:Lithium accumulates in the colon and inhibits the enzyme GSK-3β that possesses anti-carcinogenic effects. We therefore examined the association between lithium use and colorectal cancer risk in a nationwide study. METHODS:We used the Danish Cancer Registry to identify all patients diagnosed with incident co...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.10
更新日期:2016-03-01 00:00:00
abstract::Eleven human bladder carcinomata of different degrees of differentiation were implanted in mice immunosuppressed by thymectomy, anti-thymocyte serum and x-rays. Seven carcinomata grew well and one poorly and 3 produced mainly fibrous nodules in the mice. Normal human bladder tissues were grown from 4 other patients. T...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1975.30
更新日期:1975-02-01 00:00:00
abstract::The efficacy of high-dose chemotherapy (HDC) and circulating progenitor cell (CPC) transplantation in metastatic breast cancer (MBC) relies mainly on giving this treatment after a response to conventional induction chemotherapy has been achieved. For this reason an optimal mobilization regimen should be therapeuticall...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.231
更新日期:1997-01-01 00:00:00
abstract::To evaluate the predictive value of a panel of gene polymorphisms involved in metabolism of 5-FU and cisplatin on clinical outcome in advanced gastric cancer patients. A total of 52 patients were enrolled in this study. DNA was extracted from paraffin-embedded tumour specimen. Genotypes were determined using PCR-RFLP....
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602891
更新日期:2006-01-30 00:00:00
abstract::Secretary component (SC) is expressed basolaterally as a transmembrane protein (pIg receptor) on secretory epithelial cells. As pIg receptor it plays a central role in humoral immunity by mediating the external translocation of dimeric IgA and pentameric IgM. A few case reports have suggested that reduced or absent SC...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.284
更新日期:1996-06-01 00:00:00
abstract:BACKGROUND:Head and neck squamous cell carcinoma (HNSCC) is associated with poor survival. To identify prognostic and diagnostic markers and therapeutic targets, we studied ANO1, a recently identified calcium-activated chloride channel (CaCC). METHODS:High-resolution genomic and transcriptomic microarray analysis and ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605823
更新日期:2010-08-24 00:00:00
abstract:BACKGROUND:The diagnosis of neuroendocrine neoplasms (NENs) is often delayed. This first UK population-based epidemiological study of NENs compares outcomes with non-NENs to identify any inequalities. METHODS:Age-standardised incidence rate (ASR), 1-year overall survival, hazard ratios and standardised mortality rates...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-019-0606-3
更新日期:2019-11-01 00:00:00
abstract::We investigated the feasibility and the anti-tumour activity of weekly cisplatin and the simultaneous application of local hyperthermia in patients with a pelvic recurrence of cervical cancer in previously irradiated area. Dose levels of cisplatin 60 mg m(-2), 70 mg m(-2) and 80 mg m(-2) were studied. Treatment object...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/sj.bjc.6690533
更新日期:1999-07-01 00:00:00
abstract:BACKGROUND:The recently described navigator proteins have a multifaceted role in cytoskeletal dynamics. We report here on the relevance of one of them, navigator 3 (NAV3), in colorectal cancer (CRC). METHODS:We analysed changes in chromosome 12 and NAV3 copy number in CRC/adenoma samples of 59 patients and in 6 CRC ce...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.553
更新日期:2012-01-31 00:00:00
abstract:BACKGROUND:Several studies have reported discordant results regarding the impact of the CYP2D6 phenotype on both the effectiveness and the degree of endocrine symptoms associated with tamoxifen. Other studies have suggested that menopausal symptoms may be a predictive factor to tamoxifen response. METHODS:We investiga...
journal_title:British journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1038/bjc.2012.278
更新日期:2012-07-10 00:00:00
abstract::We cytogenetically investigated six osteoid osteomas, one osteoblastoma and one aggressive osteoblastoma, and observed clonal structural changes in one osteoid osteoma and in the aggressive osteoblastoma. Clonal chromosome changes had not been reported previously in osteoid osteoma, whereas the only reported chromosom...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.496
更新日期:1998-08-01 00:00:00
abstract::To investigate the efficacy and safety of combining weekly oxaliplatin with weekly 24-h infusion of high-dose 5-fluorouracil (5-FU) and folinic acid (FA) in treatment of patients with advanced gastric cancer. Patients with histologically confirmed, locally advanced or recurrent/metastatic gastric cancer were studied. ...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1038/sj.bjc.6601985
更新日期:2004-08-02 00:00:00
abstract::The brain-specific angiogenesis inhibitor 1 gene has been isolated in an attempt to find fragments with p53 "functional" binding sites. As reported herein and by others, brain-specific angiogenesis inhibitor 1 expression is present in some normal tissues, but is reduced or lost in tumour tissues. Such data and its par...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600067
更新日期:2002-02-01 00:00:00
abstract:BACKGROUND:The incidence of oesophageal adenocarcinoma is increasing globally. Barrett's oesophagus (BO) is a pre-malignant condition with no biomarker to risk stratify those at highest risk of dysplasia and malignant transformation. METHODS:Subcellular epithelial protein (HMGB1, p53, RUNX3) expression, alongside expr...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-019-0685-1
更新日期:2020-02-01 00:00:00
abstract::A serological assay for the quantitative determination of the novel tumour-associated epitope CA242 was developed and used for determination of sensitivity and specificity of CA242 in gastrointestinal cancer. The CA242 assay showed a better tumour specificity than CA50 (and CA 19-9). This was most noticeable in benign...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.44
更新日期:1992-02-01 00:00:00
abstract:BACKGROUND:We assessed the association between symptoms reported at breast cancer screening visits and interval cancers (ICs) in a prospective manner. METHODS:This population-based matched cohort study uses data of the Finnish National Breast Cancer Screening Programme that invites women aged 50-69 years old during 19...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-018-0308-2
更新日期:2018-11-01 00:00:00
abstract::Squamous cell carcinoma and adenocarcinoma of the oesophagus are cancers that develop from distinct epithelial sub-types; however, they are both related to chronic inflammation of differing aetiologies. Inflammation leads to somatically inherited genetic mutations altering control of the cell cycle, DNA replication an...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/sj.bjc.6605126
更新日期:2009-07-07 00:00:00
abstract:BACKGROUND:The pathogenesis of lymph node metastases in preinvasive breast cancer – ductal carcinoma in situ (DCIS) – remains controversial. The one-step nucleic acid amplification (OSNA) assay is a novel molecular method that can assess a whole node and detect clinically relevant metastases. In this retrospective coho...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.168
更新日期:2012-05-08 00:00:00
abstract:BACKGROUND:Twenty to 40% localised RCC patients may experience recurrence after curative surgery. Limited miRNA predictors have been identified for ccRCC recurrence. METHODS:Through a multi-phase study design, we analysed miRNAs in tissues obtained from 203 ccRCC patients. Paired t-test was used for tumour-normal comp...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.392
更新日期:2017-01-03 00:00:00
abstract::Targeting growth-regulatory pathways is a promising approach in cancer treatment. A prerequisite to the development of such therapies is characterisation of tumour growth regulation in the particular tumour cell type of interest. In order to gain insight into molecular mechanisms underlying proliferative responses in ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602535
更新日期:2005-04-25 00:00:00
abstract::Exploring the fundamental mechanisms behind the low tumour uptake of labelled monoclonal antibodies (MoAbs) during in vivo immunotargeting, experiments were performed to estimate the in vivo value of the association constant (Ka) in an experimental targeting reaction. An artificial tumour model was utilised, based on ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.219
更新日期:1992-07-01 00:00:00
abstract:BACKGROUND:CD70 is a costimulatory molecule of the tumour necrosis factor family expressed in activated immune cells and some solid tumours. In lymphocytes CD70 triggers T cell-mediated cytotoxicity and mitogen-activated protein kinase phosphorylation. METHODS:We evaluated the expression of CD70 in biopsies and melano...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2015.412
更新日期:2016-01-12 00:00:00
abstract:BACKGROUND:Heterogeneity and low incidence comprise the biggest challenge in sarcoma diagnosis and treatment. Chemotherapy, although efficient for some sarcoma subtypes, generally results in poor clinical responses and is mostly recommended for advanced disease. Specific genomic aberrations have been identified in some...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-018-0359-4
更新日期:2019-02-01 00:00:00