Participation of electrogenic Na+-K+-ATPase in the membrane potential of earthworm body wall muscles.

Abstract:

:The effect of Na+-K+-ATPase inhibitor ouabain on the resting membrane potential (Vm) was studied by glass microelectrodes in isolated somatic longitudinal muscles of the earthworm Lumbricus terrestris and compared with frog sartorius muscle. In earthworm muscle, Vm was -49 mV (inside negative) in a reference external solution with 4 mmol/l K+. The electrogenic participation of Na+-K+-ATPase was absent in solutions with very low concentrations of 0.01 mmol/l K+, higher in 4 and 8 mmol/l K+ (4-5 mV) and maximal (13 mV) in solutions containing 12 mmol/l K+ where Vm was -46 mV in the absence and -33 mV in the presence of 1 x 10(4) M ouabain. The electrogenic participation of Na+-K+-ATPase was much smaller in m. sartorius of the frog Rana temporaria bathed in 8 and 12 mmol/l K+. The results indicate that the Na+-K+-ATPase is an important electrogenic factor in earthworm longitudinal muscle fibres and that its contribution to Vm depends directly on the concentration of K+ in the bathing solution.

journal_name

Physiol Res

journal_title

Physiological research

authors

Volkov EM,Nurullin LF,Svandová I,Nikolsky EE,Vyskocil F

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

481-4

issue

4

eissn

0862-8408

issn

1802-9973

journal_volume

49

pub_type

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