Modulation of the phototoxic effect of hypericin in human leukemia CEM cell line by N-ethylmaleimide, amiloride and omeprazole.

Abstract:

:Hypericin is a photosensitizing plant pigment from Hypericum perforatum with multiple modes of light-induced biological activities due to production of singlet oxygen and/or excited-state proton transfer with consequent pH drop in the hypericin environment. In the present work, we studied the effects of three inhibitors of crucial mechanisms responsible for intracellular pH (pHi) regulation on hypericin phototoxicity: N-ethylmaleimide (NEM), an inhibitor of H+-ATPase, 5'-(N,N-dimethyl)-amiloride (DMA), an inhibitor of Na+/H+ exchanger, and omeprazole (OME), an inhibitor of H+K+-ATPase. Our experiments show that the effect of hypericin at 1 x 10(-5) and 1 x 10(-6) mol x l(-1) was significantly potentiated by NEM (1 x 10(-7)-1 x 10(--9) mol x l(-1)) and DMA (1 x 10(-6) and 1 x 10(-7) mol x l(-1)) in leukemic CEM cell line. On the other hand, OME had no significant effect on hypericin cytotoxicity. Our results support the hypothesis that the excited-state proton transfer and the consequent acidification of hypericin environment could play a role in the biological activity of hypericin.

journal_name

Physiol Res

journal_title

Physiological research

authors

Mirossay A,Mirossay L,Sarisský M,Papp P,Mojzis J

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

641-4

issue

6

eissn

0862-8408

issn

1802-9973

journal_volume

51

pub_type

杂志文章
  • Monitoring of the boron neutron-capture reaction by a simple animal model.

    abstract::Sodium borocaptate (BSH, Na2Bl2HllSH), a slow neutron-capture compound, was injected into the left forebrain ventricle of 1-week-old rats (150 microg BSH/3 microl phosphate buffered saline). After 90 min, the animals were irradiated by epithermal neutrons (LVR-15 nuclear reactor in Rez near Prague, flux density 8.8 x ...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Mares V,Lisá V,Bacáková L,Burian J

    更新日期:1997-01-01 00:00:00

  • The steroid metabolome in men with mood and anxiety disorders.

    abstract::The mood and behavior of individuals result from an orchestra of many factors. Among them steroids play an important role; however, only several common hormones have been investigated in this respect. It has been demonstrated that some steroid metabolites long considered merely the products of steroid hormone metaboli...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:10.33549/physiolres.933067

    authors: Dušková M,Hill M,Bičíková M,Šrámková M,Řípová D,Mohr P,Stárka L

    更新日期:2015-01-01 00:00:00

  • Temperature dependence of blood surface tension.

    abstract::Whole blood surface tension of 15 healthy subjects recorded by the ring method was investigated in the temperature range from 20 to 40 degrees C. The surface tension omega as a function of temperature t ( degrees C) is described by an equation of linear regression as omega(t) = (-0.473 t + 70.105) x 10(-3) N/m. Blood ...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Rosina J,Kvasnák E,Suta D,Kolárová H,Málek J,Krajci L

    更新日期:2007-01-01 00:00:00

  • Transport of an antihypoxic drug stobadine across the blood-brain barrier in rat striatum and its influence on catecholamine-oxidative current: a voltammetric study under normal and anoxic/ischaemic conditions.

    abstract::Differential pulse voltammetry with a carbon fibre microelectrode (ME) was used in pentobarbital-anaesthetized rats for monitoring the stobadine current (STB.C) on both sides of the blood-brain barrier (BBB) in the arterial bloodstream (BS) and in the corpus striatum (CS). The STB.C exhibited a distinct peak at a pola...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Pavlásek J,Haburcák M,Masánová C,Stolc S

    更新日期:1996-01-01 00:00:00

  • The role of VEGF in the diabetic patients undergoing endovascular therapy of symptomatic aortic valve stenosis.

    abstract::The aim of this study was to explore changes in plasma vascular endothelial growth factor (VEGF) in aged patients who undergone transcatheter aortic valve implantation or balloon angioplasty for the treatment of aortic stenosis. Plasma VEGF was measured in subjects with diabetes mellitus type 2 (DM) (n=21, age 79.2+/-...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:10.33549/physiolres.932857

    authors: Bláha V,Šťásek J,Bis J,Fortunato J,Andrýs C,Pavlík V,Polanský P,Brtko M,Sobotka L

    更新日期:2014-01-01 00:00:00

  • Effect of prostaglandin E2 on the ductus arteriosus in the newborn rat. An ultrastructural study.

    abstract::A patent ductus arteriosus (DA) was maintained in newborn rats (Wistar strain) by administering prostaglandin E2 (PG E2) in doses of 15 micrograms.kg-1 at 30 min intervals up to 300 min after birth. In the control animals, the DA was functionally closed 300 min after birth. The lumen was blocked by clustered endotheli...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Jarkovská D,Janatová T,Hruda J,Ostádal B,Samánek M

    更新日期:1992-01-01 00:00:00

  • Suramin affects capsaicin responses and capsaicin-noxious heat interactions in rat dorsal root ganglia neurones.

    abstract::The effect of suramin, an inhibitor of G protein regulated signalling, was studied on the membrane currents induced by noxious heat and by capsaicin in cultured dorsal root ganglia neurones isolated from neonatal rats. Whole-cell responses induced by a heat ramp (24-52 degrees C) were little affected by suramin. The n...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Vlachová V,Lyfenko A,Vyklický L,Orkand RK

    更新日期:2002-01-01 00:00:00

  • Adiponectin inhibits spontaneous and catecholamine-induced lipolysis in human adipocytes of non-obese subjects through AMPK-dependent mechanisms.

    abstract::Adiponectin is an adipokine increasing glucose and fatty acid metabolism and improving insulin sensitivity. The aim of this study was to investigate the role of adiponectin in the regulation of adipocyte lipolysis. Human adipocytes isolated from biopsies obtained during surgical operations from 16 non-obese and 17 obe...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:10.33549/physiolres.931863

    authors: Wedellová Z,Dietrich J,Siklová-Vítková M,Kološtová K,Kováčiková M,Dušková M,Brož J,Vedral T,Stich V,Polák J

    更新日期:2011-01-01 00:00:00

  • Possible mechanisms of cardiac contractile dysfunction and electrical changes in ammonium chloride induced chronic metabolic acidosis in Wistar rats.

    abstract::Metabolic acidosis could occur due to either endogenous acids accumulation or bicarbonate loss from the gastrointestinal tract or commonly from the kidney. This study aimed to investigate the possible underlying mechanism(s) of chronic acidosis-induced cardiac contractile and electrical changes in rats. Twenty four ad...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:10.33549/physiolres.933171

    authors: Lasheen NN,Mohamed GF

    更新日期:2016-12-13 00:00:00

  • Aquaporin expression in the fetal porcine urinary tract changes during gestation.

    abstract::The expression of aquaporins (AQPs) in the fetal porcine urinary tract and its relation to gestational age has not been established. Tissue samples from the renal pelvis, ureter, bladder and urethra were obtained from porcine fetuses. Samples were examined by RT-PCR (AQPs 1-11), QPCR (AQPs positive on RT-PCR), and imm...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:10.33549/physiolres.933545

    authors: Jakobsen LK,Trelborg KF,Kingo PS,Hoyer S,Andersson KE,Djurhuus JC,Norregaard R,Olsen LH

    更新日期:2018-05-04 00:00:00

  • Quantitative aspects of the fever response elicited by intracerebral injection of endotoxin in the guinea-pig.

    abstract::Fever developing after intracerebral injections of lipopolysaccharide (LPS) to guinea-pigs were monophasic, with only one peak of inner body temperature slowly developing and longlasting in a dose range 20 to 200 ng of LPS. Latency time was inversely related to the dose of LPS. Indomethacin injected to the third brain...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Spízková K,Stefl B

    更新日期:1992-01-01 00:00:00

  • How to interpret elevated plasmatic level of high-sensitive troponin T in newborns and infants?

    abstract::Research and clinical implications on novel cardiac biomarkers has intensified significantly in the past few years. The high-sensitive troponin T (hscTnT) assay plays a dominant role in diagnostic algorithm regarding myocardial injury in adults. Despite generally accepted use of hscTnT there are no data about physiolo...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:10.33549/physiolres.933704

    authors: Jehlicka P,Huml M,Rajdl D,Mockova A,Matas M,Dort J,Masopustova A

    更新日期:2018-05-04 00:00:00

  • Pattern of right ventricular pressure fall and its modulation by afterload.

    abstract::Pattern of right ventricular pressure (RVP) fall and its afterload dependence were examined by analyzing ventricular pressure curves and corresponding pressure dP/dt phase planes obtained in both ventricles in the rat heart in situ. Time and value of dP/dt(min), and the time constant tau were measured at baseline and ...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Correia-Pinto J,Henriques-Coelho T,Magalhães S,Leite-Moreira AF

    更新日期:2004-01-01 00:00:00

  • The role of ghrelin in the regulation of food intake in patients with obesity and anorexia nervosa.

    abstract::Gastrointestinal hormones play an important role in the neuroendocrine regulation of food intake and postprandial satiety. Ghrelin is a 28-amino acid orexigenic peptide produced mainly by the stomach that is involved in both the long-term regulation of body weight and the short-term regulation of postprandial satiety....

    journal_title:Physiological research

    pub_type: 杂志文章,评审

    doi:

    authors: Dostálová I,Haluzík M

    更新日期:2009-01-01 00:00:00

  • Three-dimensional electrogram in spherical coordinates: application to ischemia analysis.

    abstract::Three-dimensional electrogram was used for analysis of ischemia manifestation in isolated hearts. Three parameters based on spherical coordination system were used in this study - amplitude of electrical heart vector, its azimuth and elevation. The parameters were presented as a trend. This approach reflected ischemic...

    journal_title:Physiological research

    pub_type: 杂志文章,评审

    doi:

    authors: Janousek O,Kolárová J,Nováková M,Provazník I

    更新日期:2010-01-01 00:00:00

  • Electrocardiographic outcome of resynchronization therapy.

    abstract::Cardiac resynchronization therapy (CRT) has proven efficacious in reducing or even eliminating cardiac dyssynchrony and thus improving heart failure symptoms. However, quantification of mechanical dyssynchrony is still difficult and identification of CRT candidates is currently based just on the morphology and width o...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:10.33549/physiolres.933805

    authors: Kittnar O,Riedlbauchová L,Tomis J,LoŽek M,Valeriánová A,Hrachovina M,Mlček M,Huptych M,Janoušek J,Lhotská L

    更新日期:2017-12-30 00:00:00

  • Long-term inhibition of NO synthase induces cardiac hypertrophy with a decrease in adrenergic innervation.

    abstract::Data concerning the effect of NO on the function and structure of the heart are controversial. We have studied two main questions: (i) Does the heart muscle reflect the hypertension induced by long-term inhibition of NO synthase? (ii) Since the arginine-NO pathway is also operative in the autonomic nervous system, the...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Gerová M,Hartmannová B,Dolezel S,Jezek L

    更新日期:1996-01-01 00:00:00

  • Akt substrate of 160 kDa dephosphorylation rate is reduced in insulin-stimulated rat skeletal muscle after acute exercise.

    abstract::Because greater Akt substrate of 160 kDa (AS160) phosphorylation has been reported in insulin-stimulated skeletal muscles without improved Akt activation several hours post-exercise, we hypothesized that prior exercise would result in attenuated AS160 dephosphorylation in insulin-stimulated rat skeletal muscle. Epitro...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:10.33549/physiolres.933591

    authors: Arias EB,Wang H,Cartee GD

    更新日期:2018-03-16 00:00:00

  • Permanent depression of plasma cGMP during long-term space flight.

    abstract::The purpose of this study was to investigate plasma concentrations of cyclic guanosine monophosphate (cGMP) and atrial natriuretic peptide (ANP) during and after real and simulated space flight. Venous blood was obtained 3 min after the beginning and 2 min after the lower body negative pressure maneuver in two cosmona...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Rössler A,Noskov V,László Z,Polyakow VV,Hinghofer-Szalkay HG

    更新日期:2001-01-01 00:00:00

  • Lack of an association between apolipoprotein B XbaI polymorphism and blood lipid parameters in childhood.

    abstract::The frequencies of the alleles of XbaI polymorphism in the apolipoprotein B gene were determined in two groups of children, 82 with high (HCG) and 86 with low (LCG) cholesterol levels. A slightly higher incidence of the X2X2 genotype in HCG was found, but the differences were not statistically significant. No relation...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Hubácek JA,Pistulková H,Písa Z,Valenta Z,Skodová Z,Poledne R

    更新日期:1998-01-01 00:00:00

  • Yet another decennium of the Institute of Physiology: a dynamic interplay of innovative approaches and 60 years of tradition.

    abstract::I am pleased to introduce this special issue of Physiological Research published on the occasion of the 60th anniversary of the Institute of Physiology. It is only a second issue of this kind, the previous one being Physiological Research 53 (Suppl. 1) 2004. Since then, the Institute contributed its expertise to moder...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Kubínová L

    更新日期:2014-01-01 00:00:00

  • Mitochondrial uncoupling proteins--facts and fantasies.

    abstract::Instead of a comprehensive review, we describe the basic undisputed facts and a modest contribution of our group to the fascinating area of the research on mitochondrial uncoupling proteins. After defining the terms uncoupling, leak, protein-mediated uncoupling, we discuss the assumption that due to their low abundanc...

    journal_title:Physiological research

    pub_type: 杂志文章,评审

    doi:

    authors: Jezek P,Zácková M,Růzicka M,Skobisová E,Jabůrek M

    更新日期:2004-01-01 00:00:00

  • Down-regulation of model yeast proteins by ubiquitin-dependent proteolysis.

    abstract::Ubiquitination is a versatile tool used by all eukaryotic organisms for controlling the stability, function, and intracellular localization of a multitude of proteins. I will attempt to bring together our recent data on the down-regulation of two yeast model proteins, the galactose transporter Gal2 and fructose-1,6-bi...

    journal_title:Physiological research

    pub_type: 杂志文章,评审

    doi:

    authors: Horák J

    更新日期:2004-01-01 00:00:00

  • Absence of flow-mediated vasodilation in the rabbit femoral artery.

    abstract::The purpose of this study was to determine if there is flow-mediated vasodilation of the femoral artery in response to progressive increases in flow within a physiological range observed in the in vivo experiments. Femoral artery blood flow was determined in conscious rabbits (n = 5) using chronically implanted flowpr...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:10.33549/physiolres.931672

    authors: Clifford PS,Madden JA,Hamann JJ,Buckwalter JB,Valic Z

    更新日期:2010-01-01 00:00:00

  • Genetic predictors of the development and recurrence of Graves' disease.

    abstract::Graves' disease affects approximately 3 % of women and 0.5 % of men. The first-choice therapy is based on the administration of thyrostatic drugs. However, approximately half of patients relapse within two years of discontinuation. These patients must then decide whether to re-initiate thyrostatics, which may have ser...

    journal_title:Physiological research

    pub_type: 杂志文章,评审

    doi:10.33549/physiolres.934018

    authors: Vejrazkova D,Vcelak J,Vaclavikova E,Vankova M,Zajickova K,Duskova M,Vrbikova J,Bendlova B

    更新日期:2018-11-28 00:00:00

  • Assessment of EEG frequency dynamics using complex demodulation.

    abstract::The complex demodulation (CD) approach was applied to human EEG recorded during a cognitive task performance, including voluntary goal-directed movements. The standard CD algorithm was extended by a simple procedure using frequency histograms and power spectra to select the characteristic frequencies of EEG segments a...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Draganova R,Popivanov D

    更新日期:1999-01-01 00:00:00

  • Familial hypocalciuric hypercalcemia in an index male: grey zones of the differential diagnosis from primary hyperparathyroidism in a 13-year clinical follow up.

    abstract::Familial hypocalciuric hypercalcemia (FHH) type 1, caused by a heterozygous inactivating mutation of the gene encoding the calcium-sensing receptor (CaSR), is characterized by mild to moderate hypercalcemia, hypocalciuria and inappropriately normal or elevated parathyroid hormone (PTH). FHH must be differentiated from...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:10.33549/physiolres.934522

    authors: Zajíčková K,Dvořáková M,Moravcová J,Včelák J,Goltzman D

    更新日期:2020-09-30 00:00:00

  • Two-phase response of rat pineal melatonin to lethal whole-body irradiation with gamma rays.

    abstract::Male Wistar rats adapted to artificial light:dark (LD) regimen 12:12 h were whole-body irradiated with a single dose of 9.6 Gy of gamma rays and sham/irradiated in the night in darkness. The rats were examined 60 min, 1, 3 and 5 days after exposure between 22:00 and 01:30 h in the darkness. The results obtained indica...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Kassayová M,Ahlersová E,Ahlers I

    更新日期:1999-01-01 00:00:00

  • Glycation of myofibrillar proteins and ATPase activity after incubation with eleven sugars.

    abstract::Rat skeletal muscle myofibrils were incubated in the presence of D-glucose, D-fructose, D-galactose, D-ribose, D-tagatose, D-arabinose, D-xylose, D-mannose, L-sorbose, L-rhamnose or DL-glyceraldehyde and myofibrillar ATPase activity as well as the extent of glycation was measured. The attachment of sugars to proteins ...

    journal_title:Physiological research

    pub_type: 杂志文章

    doi:

    authors: Syrový I

    更新日期:1994-01-01 00:00:00

  • Changes in smooth muscle cell pH during hypoxic pulmonary vasoconstriction: a possible role for ion transporters.

    abstract::Hypoxic pulmonary vasoconstriction (HPV) occurs in smooth muscle cells (SMC) from small pulmonary arteries (SPA) and is accompanied by increases in free cytoplasmic calcium ([Ca2+]i) and cytoplasmic pH (pHi). SMC from large pulmonary arteries (LPA) relax during hypoxia, and [Ca2+]i and pHi decrease. Increases in pHi a...

    journal_title:Physiological research

    pub_type: 杂志文章,评审

    doi:

    authors: Madden JA,Keller PA,Kleinman JG

    更新日期:2000-01-01 00:00:00