Abstract:
:The total protein glycosylation profile and specific activity of lysosomal enzymes were investigated in rat submandibular glands isolated from very young (1-month), young (1.5-months) and adult rats (3-months) rats. The specific activity of lysosomal hydrolases (i.e. acid phosphatase, arylsulfatases A and B, beta-N-acetyl-D-glucosaminidase, beta-galactosidase and beta-glucuronidase) decreased in parallel to increasing age of the animals. Furthermore, the thermal stability of acid phosphatase and beta-N-acetyl-D-glucosaminidase was influenced by the age of rats. Age-related changes in protein profile regarding the intensity of particular bands as well as the appearance of certain proteins limited to special age groups were also demonstrated as revealed by Coomassie and lectin staining. Moreover, the marked age-related increase in structures Man (alpha1-2, alpha1-3, alpha1-6) Man, Fuc (alpha1-6) GlcNAc as well as Gal (beta1-3) GlcNAc was observed, whereas staining with terminal NeuAc and GlcNAc showed an inverse correlation. The reaction with (beta1-6) branched N-glycans and Gal (beta1-3) Gal structures was limited to 1-month-old rats. No significant changes in a specific reaction with NeuAc (alpha2-3) Gal were observed. We speculate that the observed differences with respect to protein and glycosylation profiles between 1-month-old rats and older ones could be caused by a modification of the diet composition as well as by the functional and morphological maturation of the rat submandibular gland.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Przybyło M,Lityńska A,Hoja-Łukowicz D,Kremser Esubject
Has Abstractpub_date
2004-01-01 00:00:00pages
317-26issue
3eissn
0862-8408issn
1802-9973journal_volume
53pub_type
杂志文章abstract::Stomach lesions induced by indomethacin (20 mg.kg-1 i.p.) and ethanol (1 ml 95% intragastrically) were studied after a 24 hour fast in rats which had undergone sialoadenectomy. The size of the lesions was correlated with gastric HCl secretion, with gastric vascular permeability (determined from the Evans blue concentr...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Cardiac resynchronization therapy (CRT) has proven efficacious in reducing or even eliminating cardiac dyssynchrony and thus improving heart failure symptoms. However, quantification of mechanical dyssynchrony is still difficult and identification of CRT candidates is currently based just on the morphology and width o...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933805
更新日期:2017-12-30 00:00:00
abstract::Leptin and adiponectin, two adipocytokines, may work together in regulating energy homeostasis and insulin action. Leptin gene expression has been investigated in term placental tissue complicated by gestational diabetes mellitus (GDM), but never in conjunction with all isoforms of the leptin receptor (LEPR A-D), or w...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The effect of Na+-K+-ATPase inhibitor ouabain on the resting membrane potential (Vm) was studied by glass microelectrodes in isolated somatic longitudinal muscles of the earthworm Lumbricus terrestris and compared with frog sartorius muscle. In earthworm muscle, Vm was -49 mV (inside negative) in a reference external ...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The effect of oral supplementation with glycosaminoglycans (GAG) and radical scavengers (vitamin E/selenium) on the regeneration of osteochondral defects was investigated in rabbits. After introduction of defined osteochondral defects in the knee joint, groups of ten animals were given a GAG/vitamin E/selenium mixture...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Erythrocytes (RBC) from untrained male Wistar rats and rat glomerular endothelial cells (EC) were used to investigate the effects of acute exercise (speed: 20 m/min, slope: 0, duration: 1 hour) on RBC membrane protein oxidation and adhesion to cultured EC. Experimental animals were divided into juvenile (age 10 weeks)...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::A "pulse like" increase of cytoplasmic calcium concentration, which is proportional to ionophore concentration, is induced in red cells by exposure to A23187. Different Ca2+ levels are attained depending on cellular calcium buffering power and/or primary active calcium transport activation. We examined the effect of A...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::We determined and characterized the Mg2+-dependent, Ca2+-stimulated ATPase (Ca-ATPase) activity in cell plasma membranes from the myometrium of pregnant women, and compared these characteristics to those of the active Ca2+-transport already demonstrated in this tissue. Similarly to the Ca2+-transport system, the Ca2+-...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The basis for most acute coronary events is either rupture or fissuring of unstable atherosclerotic plaques with subsequent thrombosis leading to coronary artery occlusion. The development of atherosclerotic plaques takes several decades, but the mechanical features determining its stability and the risk of rupture ca...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:
更新日期:2001-01-01 00:00:00
abstract::Because greater Akt substrate of 160 kDa (AS160) phosphorylation has been reported in insulin-stimulated skeletal muscles without improved Akt activation several hours post-exercise, we hypothesized that prior exercise would result in attenuated AS160 dephosphorylation in insulin-stimulated rat skeletal muscle. Epitro...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933591
更新日期:2018-03-16 00:00:00
abstract::We studied the circadian oscillation of lipid peroxides (TBARS) in the pineal gland of rats adapted to light:dark 12:12 h regimen. The concentration of TBARS was determined at 3-h intervals during 24 hours. TBARS of pineal gland oscillated rhythmically during the 24 h period. The maximal concentration of lipoperoxidat...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::A total genome scan and pharmacogenetic study were designed to search for genetic determinants of blood pressure (BP) as well as heart and kidney weights. Genome scanning was carried out in 266 F(2) intercrosses from Prague hypertensive hypertriglyceridemic rats for phenotypes of organ weights, baseline BP, BP after b...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Nephrotoxicity of cisplatin (CP) involves renal oxidative stress and inflammation, and sesamin (a major liganin in many plants) has strong antioxidant and antiinflammatory actions. Therefore, we investigated here the possible mitigative action of sesamin on CP nephrotoxicity in rats. Sesamin was given orally (5 mg/kg/...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.934142
更新日期:2020-02-19 00:00:00
abstract::The aim of present study was to investigate functional and physical alterations in membranes of heart mitochondria that are associated with remodeling of these organelles in acute phase of streptozotocin-induced diabetes and to elucidate the role of these changes in adaptation of the heart to acute streptozotocin-indu...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Insulin is known to increase blood flow in parallel to glucose uptake in skeletal muscle. However, it is not known if an increase in blood flow by itself is associated with an increase in glucose uptake in the absence of hyperinsulinemia. To investigate further this matter, the effect of increased blood flow on forear...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Research and clinical implications on novel cardiac biomarkers has intensified significantly in the past few years. The high-sensitive troponin T (hscTnT) assay plays a dominant role in diagnostic algorithm regarding myocardial injury in adults. Despite generally accepted use of hscTnT there are no data about physiolo...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933704
更新日期:2018-05-04 00:00:00
abstract::The main neuromodulatory methods using neurostimulation principles are described. It concerns peripheral nerve stimulation (PNS), spinal cord stimulation (SCS), deep brain stimulation (DBS), motor cortex stimulation (MSC), and repetitive transcranial magnetic stimulation (rTMS). For each method the history, pathophysi...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:10.33549/physiolres.932392
更新日期:2012-01-01 00:00:00
abstract::The potential of novel scaffold containing sodium hyaluronate, type I collagen, and fibrin was investigated in the regeneration of osteochondral defects in miniature pigs. Both autologous chondrocyte-seeded scaffolds and non-seeded scaffolds were implanted into two defects located in the non-weight-bearing zone of the...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Experimental and epidemiological studies suggest that calcium intake is inversely related to weight gain. Calcium of dairy origin has been shown to be more effective in promoting weight loss. However, clinical studies yielded controversial results concerning the role of calcium intake in weight change. The aim of this...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Hepcidin, a recently discovered antimicrobial peptide synthesized in the liver, was identified to be the key mediator of iron metabolism and distribution. Despite our knowledge of hepcidin increased in recent years, there are only limited data on hepcidin regulation during systemic inflammatory response in human subje...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.931996
更新日期:2011-01-01 00:00:00
abstract::Monoaminergic neurotransmitter 5-hydroxytryptamine (5-HT), also known as serotonin, plays important roles in modulating the function of the olfactory system. However, thus far, the knowledge about 5-HT and its receptors in olfactory receptor neurons (ORNs) and their physiological role have not been fully characterized...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932413
更新日期:2013-01-01 00:00:00
abstract::New knowledge about the neural aspects of cough has revealed a complex network of pathways that initiate cough. The effect of inflammation on cough neural processing occurs at multiple peripheral and central sites within the nervous system. Evidence exists that direct or indirect neuroimmune interaction induces a comp...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.934399
更新日期:2020-03-27 00:00:00
abstract::Continuous normobaric hypoxia (CNH) renders the heart more tolerant to acute ischemia/reperfusion injury. Protein kinase C (PKC) is an important component of the protective signaling pathway, but the contribution of individual PKC isoforms under different hypoxic conditions is poorly understood. The aim of this study ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932860
更新日期:2015-01-01 00:00:00
abstract::Adaptation to intermittent high altitude hypoxia (IHAH) increases tolerance of the isolated neonatal rat heart to ischemia and potentiates protection induced by ischemic preconditioning. In addition to the protective effect, IHAH significantly reduces growth of the animals. The aim of the present study was, therefore,...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Perinatal hypoxemia may have serious long-term effects on the adult cardiovascular system and may lead to sex-dependent changes in cardiac tolerance to acute ischemia in adult life. The aim of the study was to answer the question whether gonadectomy of the male and female rats in the early phase of ontogenetic develop...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.931833
更新日期:2010-01-01 00:00:00
abstract::We investigated and evaluated post-irradiation survival in cyclooxygenase-2-deficient (COX-2 KO) mice. Thirty-day survival following exposure of COX-2 KO mice to a lethal dose of 8.5 Gy of gamma-rays was observed to be statistically significantly lower in both males and females, as well as when the sexes were merged, ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933890
更新日期:2018-11-14 00:00:00
abstract::To compare serum adiponectin changes across the menstrual cycle between normal weight and overweight/obese young women and its correlation with serum estradiol. Young women (n=56) with regular menstrual cycle had been grouped according to their BMI into normal weight group (n=26) and overweight /obese group (n=30). Bl...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.934197
更新日期:2019-12-30 00:00:00
abstract::Gain-of-function (GOF) mutations in ion channels are rare events, which lead to increased agonist sensitivity or altered gating properties, and may render the channel constitutively active. Uncovering and following characterization of such mutants contribute substantially to the understanding of the molecular basis of...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:10.33549/physiolres.932658
更新日期:2014-01-01 00:00:00
abstract::The haemolytic action of HgCl2 (0, 15 mmol.l-1) was studied in relation to the ionic strength and concentration of glucose and saccharose in incubating medium. Blood from 94 donors, aged 19-46 years were used in our experiments. In relation to the ionic strength the haemolytic action was characteristic with two maxima...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Considerable evidence demonstrates that phenotypic switching of vascular smooth muscle cells (VSMCs) is influenced by aging and hypertension. During phenotypic switching, VSMCs undergo a switch to a proliferative and migratory phenotype, with this switch being a common pathology in cardiovascular diseases. The aim of ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933779
更新日期:2018-08-16 00:00:00