Abstract：
:Considerable evidence demonstrates that phenotypic switching of vascular smooth muscle cells (VSMCs) is influenced by aging and hypertension. During phenotypic switching, VSMCs undergo a switch to a proliferative and migratory phenotype, with this switch being a common pathology in cardiovascular diseases. The aim of this study was to explore the joint influence of age and hypertension on thoracic aortic smooth muscle phenotypic switching and the balance of Akt and mitogen-activated protein kinase (MAPK) signaling during this switch. Different ages of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY) were used to establish hypertension and aging models. The phenotypic state was determined by detecting the marker proteins alpha-SM-actin, calponin, and osteopontin (OPN) via immunohistochemical staining and Western blot. Signaling proteins associated with the Akt and MAPK pathways were detected in rat thoracic aorta using Western blot. Both aging and hypertension caused a decrease in contractile (differentiated) phenotype markers (alpha-SM-actin and calponin), while the synthetic (proliferative or de-differentiated) phenotype maker was elevated (OPN). When combining hypertension and aging, this effect was enhanced, with Akt signaling decreased, while MAPK signaling was increased. These results suggested that VSMCs phenotype switching is modulated by a balance between Akt and MAPK signaling in the process of aging and hypertension.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Zhang L,Xu Z,Wu Y,Liao J,Zeng F,Shi Ldoi
10.33549/physiolres.933779subject
Has Abstractpub_date
2018-08-16 00:00:00pages
543-553issue
4eissn
0862-8408issn
1802-9973pii
933779journal_volume
67pub_type
杂志文章abstract：:Methamphetamine (MA), as a psychostimulant drug that crosses the placental barrier, may disrupt the development of social play. The present study aims to examine the effect of prenatal MA (5 mg/kg) exposure during the first (gestational day (GD) 1-11) or second (GD 12-22) halves of prenatal development of rats on soci...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934230
更新日期：2020-04-30 00:00:00
abstract：:Young Wistar rats (aged 12, 25 and 35 days) were exposed to short-term (60 min) hypobaric hypoxia of 41 kPa. Cortical afterdischarges (ADs) were evoked by repeated direct stimulation of the sensorimotor cortex and the duration of ADs was monitored to examine the influence of magnesium sulphate injection (0.3 g/kg b.w....
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Insulin is known to increase blood flow in parallel to glucose uptake in skeletal muscle. However, it is not known if an increase in blood flow by itself is associated with an increase in glucose uptake in the absence of hyperinsulinemia. To investigate further this matter, the effect of increased blood flow on forear...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Recent results of the authors have demonstrated that the elevation of extracellular adenosine induced by the combined administration of dipyridamole, a drug inhibiting the cellular uptake of adenosine, and adenosine monophosphate (AMP), a soluble adenosine prodrug, mediates radioprotective effects in mice. Furthermore...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:The biochemical model of excitation-contraction coupling in cardiomyocyte is presented and the validity of simulations of both physiological and pathological processes is discussed. The model of regulatory and actomyosin subsystems, even if it is rather simple in its regulatory subunit, gives results well consistent w...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:This study aimed to examine relationships between DHEA(S), anthropometric parameters, oral glucose tolerance test derived data and lipid spectra in a Czech non-diabetic population. 380 healthy volunteers both with and without a family history of diabetes type 2 (DM2) were enrolled into the study (women: n=235, age 28....
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Vascular stenosis is often described only by its percentage in both clinical and scientific praxis. Previous studies gave inconclusive results regarding the effect of stenosis eccentricity on its hemodynamic effect. The aim of this experimental study was to investigate and quantify the effect of stenosis severity and ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932944
更新日期：2016-01-01 00:00:00
abstract：:Atrial fibrillation is associated with atrial remodeling, in which connexin 43 (Cx43) and cell hypertrophy play important roles. In this study, apelin-13, an aliphatic peptide, was used to explore the protective effects of the adenosine monophosphate-activated protein kinase (AMPK)/mTOR signaling pathway on Cx43 expre...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934488
更新日期：2020-11-16 00:00:00
abstract：:The aim of present study was to investigate functional and physical alterations in membranes of heart mitochondria that are associated with remodeling of these organelles in acute phase of streptozotocin-induced diabetes and to elucidate the role of these changes in adaptation of the heart to acute streptozotocin-indu...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Ubiquitination is a versatile tool used by all eukaryotic organisms for controlling the stability, function, and intracellular localization of a multitude of proteins. I will attempt to bring together our recent data on the down-regulation of two yeast model proteins, the galactose transporter Gal2 and fructose-1,6-bi...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：2004-01-01 00:00:00
abstract：:Cough is one of the most important defensive reflexes. However, extensive non- productive cough is a harmful mechanism leading to the damage of human airways. Cough is initiated by activation of vagal afferents in the airways. The site of their convergence is particularly the nucleus of the solitary tract (nTS). The s...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.934400
更新日期：2020-03-27 00:00:00
abstract：:Gastrointestinal hormones play an important role in the neuroendocrine regulation of food intake and postprandial satiety. Ghrelin is a 28-amino acid orexigenic peptide produced mainly by the stomach that is involved in both the long-term regulation of body weight and the short-term regulation of postprandial satiety....
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：2009-01-01 00:00:00
abstract：:Exposure to hyperbaric oxygen leads to increased amount of reactive oxygen species (ROS) that are derived from various sources. After the discovery that ROS can function as signaling molecules, the idea of ROS being hazardous to biological tissues has been challenged. The aim of this study was to examine the changes i...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:In this retrospective study we analysed changes of the ST segment in patients with arterial hypertension using multi-lead body surface mapping of the electric heart field as the ST segment often shows non-specific changes and is influenced by many different conditions. We constructed isointegral maps (IIM) of chosen i...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932121
更新日期：2012-01-01 00:00:00
abstract：:Increased excitability of the spinal motor system has been observed after loud and unexpected acoustic stimuli (AS) preceding H-reflexes. The paradigm has been proposed as an electrophysiological marker of reticulospinal tract activity in humans. The brainstem reticular formation also maintains dense anatomical interc...
journal_title：Physiological research
pub_type： 杂志文章,随机对照试验
doi：10.33549/physiolres.932182
更新日期：2011-01-01 00:00:00
abstract：:A "pulse like" increase of cytoplasmic calcium concentration, which is proportional to ionophore concentration, is induced in red cells by exposure to A23187. Different Ca2+ levels are attained depending on cellular calcium buffering power and/or primary active calcium transport activation. We examined the effect of A...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:This study attempted to compare the muscle fiber morphological responses to dynamic electrical muscle stimulation (DEMS) and dynamic hydraulic stimulation (DHS) in rats under hindlimb suspension (HLS). DEMS at 1 Hz, 50 Hz and 100 Hz for 10 min/day, 5 days/week were introduced to the animals' right quadriceps. Static a...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933101
更新日期：2017-07-18 00:00:00
abstract：:Pregnant rats were exposed to intermittent hypobaric hypoxia (at a simulated altitude of 7000 m or 5000 m) and the excitability of cortical neurons of their pups was tested. Stimulation of the sensorimotor cortex of rats prenatally exposed to hypoxia shortened the duration of cortical afterdischarges in 12-day-old rat...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:We investigated the effects of telmisartan, the blocker of angiotensin II receptor 1, on the regulation of systolic blood pressure (SBP) and oxidative stress through endothelial nitric oxide (NO) release in spontaneously hypertensive rats (SHRs). SHRs randomly received placebo, oral feeding of telmisartan (5 mg/kg or ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932433
更新日期：2013-01-01 00:00:00
abstract：:New knowledge about the neural aspects of cough has revealed a complex network of pathways that initiate cough. The effect of inflammation on cough neural processing occurs at multiple peripheral and central sites within the nervous system. Evidence exists that direct or indirect neuroimmune interaction induces a comp...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934399
更新日期：2020-03-27 00:00:00
abstract：:We investigated and evaluated post-irradiation survival in cyclooxygenase-2-deficient (COX-2 KO) mice. Thirty-day survival following exposure of COX-2 KO mice to a lethal dose of 8.5 Gy of gamma-rays was observed to be statistically significantly lower in both males and females, as well as when the sexes were merged, ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933890
更新日期：2018-11-14 00:00:00
abstract：:During 48 hours after application sublethal doses of hepatotoxic agent thiacetamide decrease cytochrome c oxidase activity of rat liver homogenate and isolated mitochondria to 46% and 32% of original values, respectively. This decrease may be prevented by simultaneous application of lipid emulsion (mixture of Lipofund...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The mitochondrial DNA (mtDNA) amount in cells as the basis for mitochondrial energy generating system, which produces ATP, plays an important role in the fetal development and postnatal morbidity. Isolated human cord blood leukocytes (HCBL) contribute very little to the overall metabolic turnover, but they may serve a...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Our study concerned the findings that rat and rabbit heart transplants do not survive after six hours. They become dark, hard and fail to contract within 2 min after reperfusion and never regain their function. We tested the supplementation of solutions for heart transplant preservation with tetrahydrobiopterin (H4B) ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Quaternary benzo[c]phenanthridine alkaloids (QBA), fagaronine (FA), sanguinarine (SA), chelerythrine (CHE) and the QBA extract from Macleya cordata (EX) exerted differential inhibitory effect on the hydrolytic activity of particular dipeptidyl peptidase (DPP)-like enzyme isolated from human blood plasma and from human...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Heart failure has become the most widely studied syndrome in cardiology over the recent years. Despite the encouraging achievements by angiotensin converting enzyme (ACE) inhibitors, the mortality of patients with chronic heart failure remains high. There are several factors which can potentially be responsible for th...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：1999-01-01 00:00:00
abstract：:Extensive osteolysis adjacent to orthopedic implants is often associated with wear particles of prosthetic material. The activation of the RANKL/RANK/OPG system is considered to be a likely cause of periprosthetic osteolysis leading to implant failure. The aim of this study was to examine the possible correlation betw...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:The total protein glycosylation profile and specific activity of lysosomal enzymes were investigated in rat submandibular glands isolated from very young (1-month), young (1.5-months) and adult rats (3-months) rats. The specific activity of lysosomal hydrolases (i.e. acid phosphatase, arylsulfatases A and B, beta-N-ac...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Calcium cycling is a major determinant of cardiac function. S100A1 is the most abundant member of the calcium-binding S100 protein family in myocardial tissue. S100A1 interacts with a variety of calcium regulatory proteins such as SERCA2a, ryanodine receptors, L-type calcium channels and Na(+)/Ca(2+) exchangers, thus ...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.932712
更新日期：2014-01-01 00:00:00
abstract：:Progressive compromise of antioxidant defenses and free radical-mediated lipid peroxidation, which is one of the major mechanisms of secondary traumatic brain injury (TBI), has also been reported in pediatric head trauma. In the present study, we aimed to demonstrate the effect of melatonin, which is a potent free rad...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
