Abstract：
:Quaternary benzo[c]phenanthridine alkaloids (QBA), fagaronine (FA), sanguinarine (SA), chelerythrine (CHE) and the QBA extract from Macleya cordata (EX) exerted differential inhibitory effect on the hydrolytic activity of particular dipeptidyl peptidase (DPP)-like enzyme isolated from human blood plasma and from human and rat glioma cell lines. The low-MW form of DPP-IV-like enzyme activity, corresponding most probably to DPP-8, observed only in glioma cells but not in human plasma, was inhibited preferentially by SA, CHE and EX, and only slightly by FA. The alkaloid inhibitory effect was concentration-dependent in the range 25-150 mM and directly pH-related. In addition, a subtle but consistent inhibition of the intermediate-MW form of DPP-IV-like enzyme activity, ascribed to DPP-IV/CD26, observed only in human plasma and of the attractin (high-MW form of DPP-IV-like enzyme activity, expressed in U87 glioma cells) by the studied alkaloids was observed. We conclude that some of the QBA biological effects could be determined by tissue and cell type specific dipeptidyl peptidase IV-like molecules expression pattern.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Sedo A,Malík R,Vicar J,Simánek V,Ulrichová Jsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
367-72issue
3eissn
0862-8408issn
1802-9973journal_volume
52pub_type
杂志文章abstract：:The effects of 8-days treatment with 17alpha-estradiol (33.3 microg/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was ...
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pub_type： 杂志文章
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doi：
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更新日期：2001-01-01 00:00:00
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doi：
更新日期：2002-01-01 00:00:00
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更新日期：2008-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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doi：
更新日期：1996-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2003-01-01 00:00:00
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pub_type： 杂志文章
doi：
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journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
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