Abstract:
:The potential of novel scaffold containing sodium hyaluronate, type I collagen, and fibrin was investigated in the regeneration of osteochondral defects in miniature pigs. Both autologous chondrocyte-seeded scaffolds and non-seeded scaffolds were implanted into two defects located in the non-weight-bearing zone of the femoral trochlea (defect A was located more distally and medially, defect B was located more proximally and laterally). Control defects were left untreated. Twelve weeks after the operation, the knees were evaluated in vivo using MRI. Six months after the implantation, the defects were analyzed using MRI, histological, and immunohistochemical analysis. In the A defects of chondrocyte-seeded scaffold group, hyaline cartilage and fibrocartilage was formed, containing type II collagen, acidic and neutral glycosaminoglycans while the non-seeded scaffold group was predominantly filled with fibrocartilage. Defects in the control group were predominantly filled with fibrous tissue. Histomorphometric analysis of photomicrographs revealed a significantly higher amount of hyaline cartilage in the cell-seeded scaffold group in A defects than in other groups. Both scaffold groups in A defects showed significantly less fibrous tissue than cell-seeded defects B and the control group. Both histological and MRI analysis proved that the novel composite scaffold has a potential to regenerate osteochondral defects within six months.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Filová E,Rampichová M,Handl M,Lytvynets A,Halouzka R,Usvald D,Hlucilová J,Procházka R,Dezortová M,Rolencová E,Kostáková E,Trc T,Stastný E,Kolácná L,Hájek M,Motlík J,Amler Esubject
Has Abstractpub_date
2007-01-01 00:00:00pages
S5-S16eissn
0862-8408issn
1802-9973pii
1296journal_volume
56 Suppl 1pub_type
杂志文章abstract::The present review brings the survey of the most frequently used behavioural tests in experimental models of Parkinson's disease (PD). Although there is no spontaneous occurrence of parkinsonism in animals, several experimental animal models of PD have been developed to achieve the same clinical features in animals. T...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:
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journal_title:Physiological research
pub_type: 杂志文章
doi:
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pub_type: 杂志文章,评审
doi:
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pub_type: 杂志文章
doi:10.33549/physiolres.933226
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doi:
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pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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journal_title:Physiological research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章
doi:
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pub_type: 杂志文章
doi:10.33549/physiolres.933493
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pub_type: 杂志文章
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journal_title:Physiological research
pub_type: 杂志文章
doi:
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journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.931768
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journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932324
更新日期:2012-01-01 00:00:00
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journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932968
更新日期:2015-01-01 00:00:00
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journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
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journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932259
更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
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journal_title:Physiological research
pub_type: 杂志文章
doi:
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pub_type: 杂志文章
doi:10.33549/physiolres.934118
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章
doi:
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pub_type: 杂志文章
doi:
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