Abstract:
:Certain liver metabolic diseases point to the presence of disturbances in glycogen deposition. Epinephrine raises the cAMP level that activates protein kinase A leading to the activation of phosphorylase and glycogen breakdown. In the present report, we sought to investigate whether NO is produced during adrenoceptor agonist-induced glycogenolysis in rat hepatocytes in cultures. Isolated glycogen rich rat hepatocytes in cultures were used. NO production (NO(2)(-)) was assessed under the effect of adrenergic agonists and adrenergic agonist/antagonist pairs, dibutyryl cyclic AMP sodium-potassium salt (db-cAMP), NO synthase (NOS) inhibitors N(omega)-nitro-L-arginine methyl ester (L-NAME), aminoguanidine (AG) and the NO donor S-nitroso-N-acetyl penicillamine (SNAP). The inducible NO synthase (iNOS) mRNA was examined by the reverse transcription-polymerase chain reaction (RT-PCR). Glycogenolysis was quantified by glucose levels released into medium. The amount of glucose and NO(2)(-) released by hepatocytes was increased as a result of epinephrine, phenylephrine or db-cAMP treatments. The increase in glucose and NO(2)(-) released by epinephrine or phenylephrine was blocked or reduced by prazosin pretreatment and by NOS inhibitors aminoguanidine and L-NAME. iNOS gene expression was up-regulated by epinephrine. It can be concluded that glycogenolysis occurs through -adrenoceptor stimulation and a signaling cascade may involve NO production.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Hodis J,Kutinová-Canová N,Potmesil P,Kameníková L,Kmonícková E,Zídek Z,Farghali Hsubject
Has Abstractpub_date
2007-01-01 00:00:00pages
419-25issue
4eissn
0862-8408issn
1802-9973pii
1023journal_volume
56pub_type
杂志文章abstract::Impaired cerebrovascular reactivity (CVR), an important risk factor for future stroke, is affected by a presence carotid stenosis. However, in some cases CVR can be impaired in the absence of carotid stenosis due to several poorly characterized mechanisms. We hypothesized that arterial stiffening as observed in corona...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932837
更新日期:2015-01-01 00:00:00
abstract::Progressive compromise of antioxidant defenses and free radical-mediated lipid peroxidation, which is one of the major mechanisms of secondary traumatic brain injury (TBI), has also been reported in pediatric head trauma. In the present study, we aimed to demonstrate the effect of melatonin, which is a potent free rad...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Carbon dioxide interacts both with reactive nitrogen species and reactive oxygen species. In the presence of superoxide, NO reacts to form peroxynitrite that reacts with CO2 to give nitrosoperoxycarbonate. This compound rearranges to nitrocarbonate which is prone to further reactions. In an aqueous environment, the mo...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:
更新日期:2002-01-01 00:00:00
abstract::Leptin and adiponectin, two adipocytokines, may work together in regulating energy homeostasis and insulin action. Leptin gene expression has been investigated in term placental tissue complicated by gestational diabetes mellitus (GDM), but never in conjunction with all isoforms of the leptin receptor (LEPR A-D), or w...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Circadian oscillations in biological variables in mammals are controlled by a central pacemaker in the suprachiasmatic nuclei (SCN) of the hypothalamus which coordinates circadian oscillators in peripheral tissues. The molecular clockwork responsible for this rhythmicity consists of several clock genes and their corre...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Intrauterine and perinatal life are critical periods for programming of cardiometabolic diseases. However, their relative role remains controversial. We aimed to assess, at weaning, sex-dependent alterations induced by fetal or postnatal nutritional interventions on key organs for metabolic and cardiovascular control....
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932811
更新日期:2015-01-01 00:00:00
abstract::Metabolic acidosis could occur due to either endogenous acids accumulation or bicarbonate loss from the gastrointestinal tract or commonly from the kidney. This study aimed to investigate the possible underlying mechanism(s) of chronic acidosis-induced cardiac contractile and electrical changes in rats. Twenty four ad...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933171
更新日期:2016-12-13 00:00:00
abstract::Steroids are important markers in pregnancy. Although estimating their levels separately in umbilical arterial (UA) and venous blood (UV) enable more precise insights into the functioning fetoplacental unit compared to using mixed umbilical blood (UM), selective aspiration of UA and UV is technically more demanding th...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932624
更新日期:2014-01-01 00:00:00
abstract::The purpose of this study was to investigate plasma concentrations of cyclic guanosine monophosphate (cGMP) and atrial natriuretic peptide (ANP) during and after real and simulated space flight. Venous blood was obtained 3 min after the beginning and 2 min after the lower body negative pressure maneuver in two cosmona...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::To estimate the significance of insulin in the regulation of preimplantation embryo growth, female mice received a single subdiabetogenic dose of streptozocin (65 mg/kg intraperitoneally) 8-11 days or 14-17 days before fertilization. Mean glycaemia levels and the number of embryos per mouse did not differ significantl...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Ultraviolet-radiation exerts a well-known role in the development of various ocular diseases and may contribute to the progress of age-related macular degeneration. Therefore, the use of compounds able to protect the eyes from UV-induced cellular damage is challenging. The aim of this study has been to test the protec...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933759
更新日期:2018-07-17 00:00:00
abstract::Experimental and epidemiological studies suggest that calcium intake is inversely related to weight gain. Calcium of dairy origin has been shown to be more effective in promoting weight loss. However, clinical studies yielded controversial results concerning the role of calcium intake in weight change. The aim of this...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::As an autoimmune disease, type 1 diabetes mellitus (DM) can be associated with other autoimmune disorders. The aim of this study was to detect subclinically associated autoimmune thyroid disease, coeliac disease, and Addison's disease. The presence of autoantibodies was evaluated with special regard to the control of ...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The effect of ethanol on the structural development of the central nervous system was studied in offspring of Wistar rats, drinking 20 % ethanol during pregnancy and till the 28th day of their postnatal life. The structural changes in the hippocampus and dentate gyrus were analyzed at the age of 18, 35 and 90 days. A ...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The present study was performed to evaluate the role of neuronal nitric oxide synthase (nNOS)-derived nitric oxide (NO) during the developmental phase of hypertension in transgenic rats harboring the mouse Ren-2 renin gene (TGR). The first aim of the present study was to examine nNOS mRNA expression in the renal corte...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Ferritin and increased iron stores first appeared on the list of cardiovascular risk factors more than 30 years ago and their causal role in the pathogenesis of atherosclerosis has been heavily discussed since the early 1990s. It seems that besides traditional factors such as hyperlipoproteinemia, hypertension, diabet...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:10.33549/physiolres.933589
更新日期:2017-04-05 00:00:00
abstract::A total genome scan and pharmacogenetic study were designed to search for genetic determinants of blood pressure (BP) as well as heart and kidney weights. Genome scanning was carried out in 266 F(2) intercrosses from Prague hypertensive hypertriglyceridemic rats for phenotypes of organ weights, baseline BP, BP after b...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::We studied the response of several parameters related to oxidative stress in the liver of aging rats. Male Wistar rats aged 1.5, 3, 18 and 24 months were used. Livers showed an increase in superoxide anion (O(2)(-)) concentration at 1.5 and 18 months of age compared to the 3-month-old group; a decrease in superoxide d...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.931768
更新日期:2010-01-01 00:00:00
abstract::Neuroprotective effects of estrogens and progesterone have been widely studied in various experimental models. The present study was designed to compare possible neuroprotective effects of 17alpha-estradiol, 17beta-estradiol, and progesterone on oxidative stress in rats subjected to global cerebral ischemia. Global ce...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The circadian rhythms of many behavioral and physiological functions are regulated by the major circadian pacemaker in the suprachiasmatic nucleus. Long-term opiate addiction and drug withdrawal may affect circadian rhythmicity of various hormones or the sleep/activity pattern of many experimental subjects; however, l...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933183
更新日期:2016-07-18 00:00:00
abstract::An important mechanism underlying cochlear hair cell (HC) susceptibility to hypoxia/ischemia is the influx of Ca(2+). Two main ATP-dependent mechanisms contribute to maintaining low Ca(2+) levels: uptake of Ca(2+) into intracellular stores via smooth endoplasmic reticulum calcium ATPase (SERCA) and extrusion of Ca(2+)...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Inhibition of receptor-interacting serine/threonine-protein kinase 1 (RIP1) by necrostatin-1 (Nec-1) alleviates cardiac injury due to prevention of necroptotic cell death. Its inactive analogue necrostatin-1i (Nec-1i), lacking RIP1 activity, serves as a suitable control. It is unknown if these agents influence the hea...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933393
更新日期:2016-11-23 00:00:00
abstract::In this study, we investigated the effects of N(omega)-nitro-L-arginine (L-NNA) on arterial blood pressure (BP), plasma noradrenaline (NA) and adrenaline (A) levels and angiotensin-converting enzyme (ACE) activity. L-NNA was applied with tap water (1 mg/ml) from the 3rd to the 8th week of age (group L-NNA1). In Experi...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The aim of this study was to explore changes in plasma vascular endothelial growth factor (VEGF) in aged patients who undergone transcatheter aortic valve implantation or balloon angioplasty for the treatment of aortic stenosis. Plasma VEGF was measured in subjects with diabetes mellitus type 2 (DM) (n=21, age 79.2+/-...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932857
更新日期:2014-01-01 00:00:00
abstract::We investigated the effects of telmisartan, the blocker of angiotensin II receptor 1, on the regulation of systolic blood pressure (SBP) and oxidative stress through endothelial nitric oxide (NO) release in spontaneously hypertensive rats (SHRs). SHRs randomly received placebo, oral feeding of telmisartan (5 mg/kg or ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932433
更新日期:2013-01-01 00:00:00
abstract::This study attempted to compare the muscle fiber morphological responses to dynamic electrical muscle stimulation (DEMS) and dynamic hydraulic stimulation (DHS) in rats under hindlimb suspension (HLS). DEMS at 1 Hz, 50 Hz and 100 Hz for 10 min/day, 5 days/week were introduced to the animals' right quadriceps. Static a...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933101
更新日期:2017-07-18 00:00:00
abstract::In this work, we evaluated the effect of adaptation to heat on the fall of blood pressure (BP) induced by heat shock (HS) and the interrelation between nitric oxide (NO) and heat shock protein, HSP70. Experiments were carried out on Wistar rats. It was shown that HS resulted in a generalized and transient increase in ...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Stomach lesions induced by indomethacin (20 mg.kg-1 i.p.) and ethanol (1 ml 95% intragastrically) were studied after a 24 hour fast in rats which had undergone sialoadenectomy. The size of the lesions was correlated with gastric HCl secretion, with gastric vascular permeability (determined from the Evans blue concentr...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Neutral trehalase 1 (Nth1) from Saccharomyces cerevisiae catalyzes disaccharide trehalose hydrolysis and helps yeast to survive adverse conditions, such as heat shock, starvation or oxidative stress. 14-3-3 proteins, master regulators of hundreds of partner proteins, participate in many key cellular processes. Nth1 is...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:10.33549/physiolres.933950
更新日期:2019-04-30 00:00:00
abstract::Gain-of-function (GOF) mutations in ion channels are rare events, which lead to increased agonist sensitivity or altered gating properties, and may render the channel constitutively active. Uncovering and following characterization of such mutants contribute substantially to the understanding of the molecular basis of...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:10.33549/physiolres.932658
更新日期:2014-01-01 00:00:00