Abstract:
:We investigated the effects of telmisartan, the blocker of angiotensin II receptor 1, on the regulation of systolic blood pressure (SBP) and oxidative stress through endothelial nitric oxide (NO) release in spontaneously hypertensive rats (SHRs). SHRs randomly received placebo, oral feeding of telmisartan (5 mg/kg or 10 mg/kg) every day and Wistar-Kyoto rats (WKYs) served as normotensive control. The SBP of rat was measured before and weekly thereafter. After a total of 8-week treatment, rats were killed for experimental measurements. Parameters that subject to measurements in isolated aorta endothelial cells include: NO concentration, protein expression levels of angiotensin II receptor 1, nitrotyrosine, 8-isoprostane, SOD, PI3K, Akt, AMPK and eNOS. In addition, L-NMMA, a general inhibitor of nitric oxide synthase, was also applied to test the inhibition of NO concentration. We found that SBPs were significantly lower in telmisartan therapy group than in placebo treated hypertensive rats and WKYs (p<0.05). The NO concentration was significantly higher in telmisartan-treated group with increased activity of the PI3K/Akt pathway and activated eNOS signaling. Blockade of Akt activity reversed such effects. Activation of AMPK also contributed to the phosphorylation of eNOS. L-NMMA treatment reduced less NO concentration in SHR rats than the telmisartan co-treated groups. Oxidative stress in SHRs was also attenuated by telmisartan administration, shown by reduced formation of nitrotyrosine, 8-isoprostane, and recovered SOD protein level. Telmisartan enhanced NO release by activating the PI3K/Akt system, AMPK phosphorylation and eNOS expression, which attenuated the blood pressure and oxidative stress in SHRs.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Xu L,Liu Ydoi
10.33549/physiolres.932433subject
Has Abstractpub_date
2013-01-01 00:00:00pages
351-9issue
4eissn
0862-8408issn
1802-9973pii
932433journal_volume
62pub_type
杂志文章abstract::Wire myograph is a device for the in vitro investigation of both, active and passive properties of arteries. Arteries from a variety of animal species, pathological states, and vascular beds were investigated using this method. We focus on the normalization procedure which is aimed to standardize experimental settings...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::We measured plasma concentrations, adipose tissue and placental mRNA expression of hepatokines fetuin A, fetuin B and fibroblast growth factor 21 (FGF21) in 12 healthy pregnant women (P group), 12 pregnant women with gestational diabetes (GDM) and 10 healthy non-pregnant women (N group) to explore their potential role...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.934017
更新日期:2018-11-28 00:00:00
abstract::Impaired cerebrovascular reactivity (CVR), an important risk factor for future stroke, is affected by a presence carotid stenosis. However, in some cases CVR can be impaired in the absence of carotid stenosis due to several poorly characterized mechanisms. We hypothesized that arterial stiffening as observed in corona...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932837
更新日期:2015-01-01 00:00:00
abstract::The expression of aquaporins (AQPs) in the fetal porcine urinary tract and its relation to gestational age has not been established. Tissue samples from the renal pelvis, ureter, bladder and urethra were obtained from porcine fetuses. Samples were examined by RT-PCR (AQPs 1-11), QPCR (AQPs positive on RT-PCR), and imm...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933545
更新日期:2018-05-04 00:00:00
abstract::Spontaneous activity of cortical neurons exhibits alternative fluctuations of membrane potential consisting of phased depolarization called "up-state" and persistent hyperpolarization called "down-state" during slow wave sleep and anesthesia. Here, we examined the effects of sound stimuli (noise bursts) on neuronal ac...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932086
更新日期:2011-01-01 00:00:00
abstract::In order to examine the relationship between certain risk factors for atherosclerosis and family history of myocardial infarction, we compared a group of children (n = 51) whose parents had survived myocardial infarction (n = 34) with a control group of children (n = 90) with a negative family history of atheroscleros...
journal_title:Physiological research
pub_type: 临床试验,杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Metabolic syndrome is a prevalent, complex condition. The search for genetic determinants of the syndrome is currently undergoing a paradigm enhancement by adding systems genetics approaches to association studies. We summarize the current evidence on relations between an emergent new candidate, zinc finger and BTB do...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:10.33549/physiolres.933730
更新日期:2017-09-26 00:00:00
abstract::The aim of the present work was to investigate a new mechanism likely contributing to the toxic action of acetaminophen, especially to explore the possible inhibition of glutathione reductase through an acetaminophen-glutathione conjugate (APAP-SG). APAP-SG conjugate was synthesized by organic synthesis and purified b...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.931744
更新日期:2010-01-01 00:00:00
abstract::Neuroprotective effects of estrogens and progesterone have been widely studied in various experimental models. The present study was designed to compare possible neuroprotective effects of 17alpha-estradiol, 17beta-estradiol, and progesterone on oxidative stress in rats subjected to global cerebral ischemia. Global ce...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The objective of the present experiment was to assess the involvement of small intestine in expression of susceptibility or resistance to the high-fat/high-energy diet. The investigation was carried out in adult male Sprague-Dawley rats fed either standard laboratory diet (3.2 kcal/g, 9.5 % fat) or high-fat (HF) diet ...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The effect of oral supplementation with glycosaminoglycans (GAG) and radical scavengers (vitamin E/selenium) on the regeneration of osteochondral defects was investigated in rabbits. After introduction of defined osteochondral defects in the knee joint, groups of ten animals were given a GAG/vitamin E/selenium mixture...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::The method of cellular immobilization and perfusion was applied to adipocytes. The lipolytic effect of isoprenaline, whose action is produced as a result of receptor-drug interaction, was followed. An agarose solution kept at at 37 degrees C was mixed 1:1 with the cell suspension. Thereafter, adipocytes were immobiliz...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Exposure to hyperbaric oxygen leads to increased amount of reactive oxygen species (ROS) that are derived from various sources. After the discovery that ROS can function as signaling molecules, the idea of ROS being hazardous to biological tissues has been challenged. The aim of this study was to examine the changes i...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::As an autoimmune disease, type 1 diabetes mellitus (DM) can be associated with other autoimmune disorders. The aim of this study was to detect subclinically associated autoimmune thyroid disease, coeliac disease, and Addison's disease. The presence of autoantibodies was evaluated with special regard to the control of ...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Ubiquitination is a versatile tool used by all eukaryotic organisms for controlling the stability, function, and intracellular localization of a multitude of proteins. I will attempt to bring together our recent data on the down-regulation of two yeast model proteins, the galactose transporter Gal2 and fructose-1,6-bi...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:
更新日期:2004-01-01 00:00:00
abstract::Ischemic postconditioning and remote conditioning are potentially useful tools for protecting ischemic myocardium. This study tested the hypothesis that 2,3-dehydrosilybin (DHS), a flavonolignan component of Silybum marianum, could attenuate cardiomyocyte damage following hypoxia/reoxygenation by decreasing the genera...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932703
更新日期:2015-01-01 00:00:00
abstract::To estimate the significance of insulin in the regulation of preimplantation embryo growth, female mice received a single subdiabetogenic dose of streptozocin (65 mg/kg intraperitoneally) 8-11 days or 14-17 days before fertilization. Mean glycaemia levels and the number of embryos per mouse did not differ significantl...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Experiments were performed in C57BL/6J male mice to determine the effects of acetylcholinesterase (AChE) inhibitor pyridostigmine bromide (PB) and stress on cardiovascular function, structure, and apoptosis. Mice were studied for seven days under the following conditions: Controls (osmotic minipump with saline), PB (1...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::In this study we analyzed the effects of melatonin (Mel, 1 mg/kg ip) on behavioral changes as well as cell and oxidative damage prompted by bilaterally olfactory bulbectomy. Olfactory bulbectomy caused an increase in lipid peroxidation products and caspase-3, whereas it prompted a decrease of reduced glutathione (GSH)...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.931684
更新日期:2010-01-01 00:00:00
abstract::The concept of vena contracta space reduction in tricuspid valve position was tested in an animal model. Feasibility of specific artificial obturator body (REMOT) fixed to the right ventricular apex and interacting with tricuspid valve leaflets was evaluated in three different animal studies. Catheter-based technique ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932580
更新日期:2014-01-01 00:00:00
abstract::The haemolytic action of HgCl2 (0, 15 mmol.l-1) was studied in relation to the ionic strength and concentration of glucose and saccharose in incubating medium. Blood from 94 donors, aged 19-46 years were used in our experiments. In relation to the ionic strength the haemolytic action was characteristic with two maxima...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Store-operated calcium entry (SOCE) is one of regulatory mechanisms which regulates Ca2+ cycling in the heart. SOCE alterations in pathological conditions contribute to progression of heart failure and cardiac hypertrophy by multiple signaling pathways such as Cn/NFAT and CaMKII/MEF2. Several components mediating SOCE...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.934300
更新日期:2019-11-30 00:00:00
abstract::MiRNAs are important regulators of gene expression and changes in their levels are linked with various pathological states, including solid tumors. MiR-215 has been identified as a tumor suppressor in colorectal cancer (CRC). Following our previous in vitro and in vivo experiments, the aim of this project was to study...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933546
更新日期:2016-12-21 00:00:00
abstract::We investigated whether polyethylene glycol-coated Fe3O4 nanoparticles (IONs), acute stress and their combination modifies vascular functions, nitric oxide synthase (NOS) activity, mean arterial pressure (MAP) as well as hepcidin and ferritin H gene expressions in Wistar-Kyoto rats. Rats were divided into control, ION...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.934567
更新日期:2020-12-22 00:00:00
abstract::The aim of this study was to assess carotid baroreflex responses during graded lower body negative pressure (LBNP). In 12 healthy subjects (age 29+/-4 years) we applied sinusoidal neck suction (0 to -30 mmHg) at 0.1 Hz to examine the sympathetic modulation of the heart and blood vessels and at 0.2 Hz to assess the eff...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::TGF-beta superfamily members including bone morphogenetic proteins (BMPs) and their receptors (BMPR-1A, -1B and -2) have been shown to be important for reproductive function in both males and females, while information on the role of BMPs in males is limited. Functional studies on select BMPs and BMP receptors have de...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933193
更新日期:2016-11-08 00:00:00
abstract::The aim of this study was to investigate the effects of rooibos tea as a natural source of a wide scale of antioxidants on the prevention and treatment of oxidative stress in streptozotocin-induced diabetic rats. Expected significant changes of biochemical parameters characteristic for experimental diabetic state were...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Mammalian Meckel´s cartilage is a temporary structure associated with mandible development. Notably, its elimination is not executed by apoptosis, and autophagy was suggested as the major mechanism. Simultaneous reports point to pro-apoptotic caspases as novel participants in autophagic pathways in general. The aim of...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933947
更新日期:2019-03-06 00:00:00
abstract::Decrease of arterial wall shear stress (WSS) is associated with higher probability of atherosclerotic plaque development in many disease conditions. End-stage renal diseases (ESRD) patients suffer from vascular disease frequently, but its nature differs from general population. This study was aimed at proving an assoc...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932259
更新日期:2012-01-01 00:00:00
abstract::Leptin and adiponectin, two adipocytokines, may work together in regulating energy homeostasis and insulin action. Leptin gene expression has been investigated in term placental tissue complicated by gestational diabetes mellitus (GDM), but never in conjunction with all isoforms of the leptin receptor (LEPR A-D), or w...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00