Abstract：
:Mammalian Meckel´s cartilage is a temporary structure associated with mandible development. Notably, its elimination is not executed by apoptosis, and autophagy was suggested as the major mechanism. Simultaneous reports point to pro-apoptotic caspases as novel participants in autophagic pathways in general. The aim of this research was to find out whether activation of pro-apoptotic caspases (-2, -3, -6, -7, -8 and -9) was associated with autophagy of the Meckel´s cartilage chondrocytes. Active caspases were examined in serial histological sections of mouse mandible using immunodetection and were correlated with incidence of autophagy based on Beclin-1 expression. Caspase-2 and caspase-8 were found in Beclin-1 positive regions, whereas caspase-3, -6, -7 and -9 were not present. Caspase-8 was further correlated with Fas/FasL and HIF-1alpha, potential triggers for its activation. Some Fas and FasL positivity was observed in the chondrocytes but caspase-8 activation was found also in FasL deficient cartilage. HIF-1alpha was abundantly present in the hypertrophic chondrocytes. Taken together, caspase-8 activation in the Meckel´s cartilage was demonstrated for the first time. Caspase-8 and caspase-2 were the only pro-apoptotic caspases detected in the Beclin-1 positive segment of the cartilage. Activation of caspase-8 appears FasL/Fas independent but may be switched on by HIF-1alpha.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Bíliková P,Švandová E,Veselá B,Doubek J,Poliard A,Matalová Edoi
10.33549/physiolres.933947subject
Has Abstractpub_date
2019-03-06 00:00:00pages
135-140issue
1eissn
0862-8408issn
1802-9973pii
933947journal_volume
68pub_type
杂志文章abstract：:Glucose tolerance, insulin binding to erythrocytes, insulinaemia, plasma total cholesterol, plasma triglycerides, weight of fat pads, food consumption and body weight changes were studied in genetically hypertensive lean Koletsky rats. Long-term treatment with dopaminergic agonist terguride (0.2 mg/kg/day) normalized ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:The present study investigated the effects of head cooling during endurance cycling on performance and the serotonergic neuroendocrine response to exercise in the heat. Subjects exercised at 75 % VO(2max) to volitional fatigue on a cycle ergometer at an ambient temperature of 29+/-1.0 degrees C, with a relative humidi...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:The lymphatic bioavailability (FL) of diazepam (DZ) and its major metabolite desmethyldiazepam (DDZ) was studied. DZ was administered in intravenous and intraduodenal boluses, and in intravenous infusion in three groups of rats with different total lipid (TL) content in the central lymph. The effect of a) different li...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:The effects of inclusion of sesamin / episesamin in Baltic Atlantic salmon (Salmo salar L.) diets based on vegetable oils were studied. The study was designed as a dose response study with two control diets, one diet based on fish oil (FO) and one diet based on a mixture of linseed and sunflower oil (6:4 by vol.) (MO)...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932068
更新日期：2011-01-01 00:00:00
abstract：:The aim of this study was to assess the molecular basis of renal Na,K-ATPase disturbances in response to NO-deficient hypertension induced in rats by NO-synthase inhibition with 40 mg/kg/day N(G)-nitro-L-arginine methyl ester (L-NAME) for four weeks. After 4-week administration of L-NAME, the systolic blood pressure (...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Chronic kidney disease (CKD) is associated with increased concentration of intracellular calcium, which is pathological and may lead to irreversible damage of cell functions and structures. The aim of our study was to investigate the impact of 6 months vitamin D(3) supplementation (14 000 IU/week) on free cytosolic ca...
journal_title：Physiological research
pub_type： 临床试验,杂志文章
doi：10.33549/physiolres.932930
更新日期：2014-01-01 00:00:00
abstract：:Exposure to hyperbaric oxygen leads to increased amount of reactive oxygen species (ROS) that are derived from various sources. After the discovery that ROS can function as signaling molecules, the idea of ROS being hazardous to biological tissues has been challenged. The aim of this study was to examine the changes i...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:We sought to examine the association between maternal erythrocyte omega-3, omega-6 and trans fatty acids and risk of preeclampsia. We conducted a case-control study of 170 women with proteinuric, pregnancy-induced hypertension and 185 normotensive pregnant women who delivered at Harare Maternity Hospital, Harare, Zimb...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:Extensive osteolysis adjacent to orthopedic implants is often associated with wear particles of prosthetic material. The activation of the RANKL/RANK/OPG system is considered to be a likely cause of periprosthetic osteolysis leading to implant failure. The aim of this study was to examine the possible correlation betw...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:The complex demodulation (CD) approach was applied to human EEG recorded during a cognitive task performance, including voluntary goal-directed movements. The standard CD algorithm was extended by a simple procedure using frequency histograms and power spectra to select the characteristic frequencies of EEG segments a...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Osteoporotic fractures are the result of low density and especially inferior bone quality (microarchitecture) caused by both internal (genes, hormones) and external (life style) influences. Bone mechanosensors are extremely important for the overall integrity of the skeleton, because in response to mechanical load the...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：2008-01-01 00:00:00
abstract：:The aim of the study was to assess the association between promoter polymorphism [A(-596)G] in interleukin-6 gene and office systolic and diastolic blood pressures, and the heart rate (HR) in apparently healthy Czech subjects. Furthermore, we evaluated the possible influence of gender, BMI and smoking on these suppose...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:In this study we investigated the effect of beta-glucan derived from Saccharomyces cerevisiae on fungicidal activity, cytokine production and natural killer activity. Spleen and peritoneal cells from female C57BL/6 mice, previously injected (24 or 48 h) with 20 or 100 microg of glucan by i.p. route, were assayed. In v...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:This study was designated to estimate protective role of ETA and ETB receptor antagonist against endothelin 1 (ET-1)-induced oxidative stress in lungs and determine whether these effects are mediated by nitric oxide (NO) synthase. Experiments were performed on Wistar rats divided into the following groups: I - saline ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933263
更新日期：2016-11-23 00:00:00
abstract：:In this report, we dealt with ventricular activation abnormalities in 30 patients with previous non-Q myocardial infarction (MI) by means of the CARDIAG 128.1 device, which enables analysis of ECGs, VCGs and body surface potential maps. The diagnosis was verified by left ventriculography, echocardiography and perfusio...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Methamphetamine is one of the most abused hard drugs in the Czech Republic. Its popularity is high not only in Eastern Bloc of Europe but is growing in other countries around the world, including the United States. In addition, methamphetamine abuse increases in drug addicts during pregnancy. Although research into th...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.934360
更新日期：2019-12-20 00:00:00
abstract：:Argyrophil nucleolar organizer regions (AgNORs) were morphometrically evaluated in rat anterior pituitaries of control and oestrogenized rats using an automatized (LUCIA M--Laboratory Universal Computer Image Analysis Micro) and a non-automatized approaches to investigate not only the sensitivity and effectiveness of ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:The objective of this study was to evaluate the effect of diet and 677 C-->T mutation of the methylenetetrahydrofolate reductase (MTHFR) gene on plasma homocyst(e)ine concentrations in an adolescent population (113 males, age: 14.2+/-2.4 years; 202 females, age: 14.9+/-2.1 years) from a region characterized by high ca...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:We used a rat model to assess the role of nephrin, podocin, and desmin in the pathogenesis of IgA nephropathy (IgAN). A rat IgAN model was established by administration of BSA, CCl(4), and lipopolysaccharide (LPS) and compared with healthy control rats. Urinary protein, urine red blood cells, and biochemical parameter...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932380
更新日期：2013-01-01 00:00:00
abstract：:Sexually mature female Wistar rats were given daily intragastric doses of ethinylestradiol (EE) and levonorgestrel (LE) used normally in women: (1) 0.03 mg EE and 0.05 mg LE; (2) 0.04 mg EE and 0.075 mg LE; (3) 0.03 mg EE and 0.125 mg LE. All groups were treated for 6 months in 5-day cycles (four-day treatment with a ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:To compare serum adiponectin changes across the menstrual cycle between normal weight and overweight/obese young women and its correlation with serum estradiol. Young women (n=56) with regular menstrual cycle had been grouped according to their BMI into normal weight group (n=26) and overweight /obese group (n=30). Bl...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934197
更新日期：2019-12-30 00:00:00
abstract：:A patent ductus arteriosus (DA) was maintained in newborn rats (Wistar strain) by administering prostaglandin E2 (PG E2) in doses of 15 micrograms.kg-1 at 30 min intervals up to 300 min after birth. In the control animals, the DA was functionally closed 300 min after birth. The lumen was blocked by clustered endotheli...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:The mitochondrial DNA (mtDNA) amount in cells as the basis for mitochondrial energy generating system, which produces ATP, plays an important role in the fetal development and postnatal morbidity. Isolated human cord blood leukocytes (HCBL) contribute very little to the overall metabolic turnover, but they may serve a...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Pattern of right ventricular pressure (RVP) fall and its afterload dependence were examined by analyzing ventricular pressure curves and corresponding pressure dP/dt phase planes obtained in both ventricles in the rat heart in situ. Time and value of dP/dt(min), and the time constant tau were measured at baseline and ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:The present study was designed to measure interstitial levels of norepinephrine-regulating lipolysis (NE) in subcutaneous abdominal adipose tissue of anorexia nervosa (AN) patients and control subjects under basal conditions and after the local administration of an inhibitor of NE re-uptake, maprotiline. In vivo micro...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:A "pulse like" increase of cytoplasmic calcium concentration, which is proportional to ionophore concentration, is induced in red cells by exposure to A23187. Different Ca2+ levels are attained depending on cellular calcium buffering power and/or primary active calcium transport activation. We examined the effect of A...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Ischemic postconditioning and remote conditioning are potentially useful tools for protecting ischemic myocardium. This study tested the hypothesis that 2,3-dehydrosilybin (DHS), a flavonolignan component of Silybum marianum, could attenuate cardiomyocyte damage following hypoxia/reoxygenation by decreasing the genera...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932703
更新日期：2015-01-01 00:00:00
abstract：:Store-operated calcium entry (SOCE) is one of regulatory mechanisms which regulates Ca2+ cycling in the heart. SOCE alterations in pathological conditions contribute to progression of heart failure and cardiac hypertrophy by multiple signaling pathways such as Cn/NFAT and CaMKII/MEF2. Several components mediating SOCE...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934300
更新日期：2019-11-30 00:00:00
abstract：:Rat skeletal muscle myofibrils were incubated in the presence of D-glucose, D-fructose, D-galactose, D-ribose, D-tagatose, D-arabinose, D-xylose, D-mannose, L-sorbose, L-rhamnose or DL-glyceraldehyde and myofibrillar ATPase activity as well as the extent of glycation was measured. The attachment of sugars to proteins ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The effect of oral supplementation with glycosaminoglycans (GAG) and radical scavengers (vitamin E/selenium) on the regeneration of osteochondral defects was investigated in rabbits. After introduction of defined osteochondral defects in the knee joint, groups of ten animals were given a GAG/vitamin E/selenium mixture...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
