Abstract:
:The expression of aquaporins (AQPs) in the fetal porcine urinary tract and its relation to gestational age has not been established. Tissue samples from the renal pelvis, ureter, bladder and urethra were obtained from porcine fetuses. Samples were examined by RT-PCR (AQPs 1-11), QPCR (AQPs positive on RT-PCR), and immunohistochemistry. Bladder samples were additionally examined by Western blotting. RNA was extracted from 76 tissue samples obtained from 19 fetuses. Gestational age was 60 (n=11) or 100 days (n=8). PCR showed that AQP1, 3, 9 and 11 mRNA was expressed in all locations. The expression of AQP3 increased significantly at all four locations with gestational age, whereas AQP11 significantly decreased. AQP1 expression increased in the ureter, bladder and urethra. AQP9 mRNA expression increased in the urethra and bladder, but decreased in the ureter. AQP5 was expressed only in the urethra. Immunohistochemistry showed AQP1 staining in sub-urothelial vessels at all locations. Western blotting analysis confirmed increased AQP1 protein levels in bladder samples during gestation. Expression levels of AQP1, 3, 5, 9 and 11 in the urinary tract change during gestation, and further studies are needed to provide insights into normal and pathophysiological water handling mechanisms in the fetus.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Jakobsen LK,Trelborg KF,Kingo PS,Hoyer S,Andersson KE,Djurhuus JC,Norregaard R,Olsen LHdoi
10.33549/physiolres.933545subject
Has Abstractpub_date
2018-05-04 00:00:00pages
283-292issue
2eissn
0862-8408issn
1802-9973pii
933545journal_volume
67pub_type
杂志文章abstract::TGF-beta superfamily members including bone morphogenetic proteins (BMPs) and their receptors (BMPR-1A, -1B and -2) have been shown to be important for reproductive function in both males and females, while information on the role of BMPs in males is limited. Functional studies on select BMPs and BMP receptors have de...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933193
更新日期:2016-11-08 00:00:00
abstract::The aim of this study was to investigate the effects of rooibos tea as a natural source of a wide scale of antioxidants on the prevention and treatment of oxidative stress in streptozotocin-induced diabetic rats. Expected significant changes of biochemical parameters characteristic for experimental diabetic state were...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Vision is a fascinating example of the interaction of a biological system with the outside world. The first step of translating electromagnetic energy into a biologically recognizable signal involves the phototransduction cascade in retinal photoreceptor cells. Phototransduction is the best studied example of a GTP bi...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:
更新日期:1998-01-01 00:00:00
abstract::The mood and behavior of individuals result from an orchestra of many factors. Among them steroids play an important role; however, only several common hormones have been investigated in this respect. It has been demonstrated that some steroid metabolites long considered merely the products of steroid hormone metaboli...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933067
更新日期:2015-01-01 00:00:00
abstract::Spontaneous activity of cortical neurons exhibits alternative fluctuations of membrane potential consisting of phased depolarization called "up-state" and persistent hyperpolarization called "down-state" during slow wave sleep and anesthesia. Here, we examined the effects of sound stimuli (noise bursts) on neuronal ac...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932086
更新日期:2011-01-01 00:00:00
abstract::The method of cellular immobilization and perfusion was applied to adipocytes. The lipolytic effect of isoprenaline, whose action is produced as a result of receptor-drug interaction, was followed. An agarose solution kept at at 37 degrees C was mixed 1:1 with the cell suspension. Thereafter, adipocytes were immobiliz...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Plant-based n-3 polyunsaturated fatty acids (PUFA) possess a prospective antiatherogenic potential. Currant oil from Ribes nigrum L. is one of the few plant oils containing PUFAn-3 (15.3 mol%) in addition to PUFAn-6 (60.5 mol%). This study was aimed at comparing the effects of currant oil with those of lard fat, rich ...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::The frequencies of the alleles of XbaI polymorphism in the apolipoprotein B gene were determined in two groups of children, 82 with high (HCG) and 86 with low (LCG) cholesterol levels. A slightly higher incidence of the X2X2 genotype in HCG was found, but the differences were not statistically significant. No relation...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Eukaryotic cytochrome c oxidase (CcO), the terminal component of the mitochondrial electron transport chain is a heterooligomeric complex that belongs to the superfamily of heme-copper containing terminal oxidases. The enzyme, composed of both mitochondrially and nuclear encoded subunits, is embedded in the inner mito...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
abstract::Graves' disease affects approximately 3 % of women and 0.5 % of men. The first-choice therapy is based on the administration of thyrostatic drugs. However, approximately half of patients relapse within two years of discontinuation. These patients must then decide whether to re-initiate thyrostatics, which may have ser...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:10.33549/physiolres.934018
更新日期:2018-11-28 00:00:00
abstract::Hepcidin, a recently discovered antimicrobial peptide synthesized in the liver, was identified to be the key mediator of iron metabolism and distribution. Despite our knowledge of hepcidin increased in recent years, there are only limited data on hepcidin regulation during systemic inflammatory response in human subje...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.931996
更新日期:2011-01-01 00:00:00
abstract::The goal of this study is to evaluate if promotion of angiogenesis by systemic treatment with an antagomir against miR-92a, a well established inhibitor of angiogenesis, will maximize the benefits of exercise on bone. Ten week old female C57BL6/J mice were subjected to two weeks of external load by four point bending....
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932410
更新日期:2013-01-01 00:00:00
abstract::Silymarin and silybin are widely used for their hepatoprotective properties. Our previous studies confirm positive effect of silymarin on lipoprotein profile and lipid homeostasis. Advanced drug forms may improve the bioavailability of these compounds. In this study, we investigate the effects of silybin in different ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933235
更新日期:2015-01-01 00:00:00
abstract::The GH4C1 pituitary cell line, an excellent model for a thyroid hormone action study, was used for determination of the relationship between thyroid hormone receptor occupancy and intensity of cell proliferation, prolactin (PRL) production, thyrotropin (TSH) inhibition and 3,5,3,-L-triiodothyronine (T3) receptor down-...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Cardiovascular disease, while rare in women of reproductive age, is the main cause of mortality in menopause. The purpose of our study was to determine the association of natural menopause with cardiovascular risk factors, including their clustering into metabolic syndrome (MS). A random 5 % representative population ...
journal_title:Physiological research
pub_type: 杂志文章,随机对照试验
doi:10.33549/physiolres.932313
更新日期:2012-01-01 00:00:00
abstract::Differential pulse voltammetry with a carbon fibre microelectrode (ME) was used in pentobarbital-anaesthetized rats for monitoring the stobadine current (STB.C) on both sides of the blood-brain barrier (BBB) in the arterial bloodstream (BS) and in the corpus striatum (CS). The STB.C exhibited a distinct peak at a pola...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Argyrophil nucleolar organizer regions (AgNORs) were morphometrically evaluated in rat anterior pituitaries of control and oestrogenized rats using an automatized (LUCIA M--Laboratory Universal Computer Image Analysis Micro) and a non-automatized approaches to investigate not only the sensitivity and effectiveness of ...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Hypersensitive pain response is observed in patients with Parkinson's disease (PD). However, the signal pathways leading to hyperalgesia still need to be clarified. Chronic oxidative stress is one of the hallmarks of PD pathophysiology. Since the midbrain periaqueductal gray (PAG) is an important component of the desc...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.934478
更新日期:2020-08-31 00:00:00
abstract::The main neuromodulatory methods using neurostimulation principles are described. It concerns peripheral nerve stimulation (PNS), spinal cord stimulation (SCS), deep brain stimulation (DBS), motor cortex stimulation (MSC), and repetitive transcranial magnetic stimulation (rTMS). For each method the history, pathophysi...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:10.33549/physiolres.932392
更新日期:2012-01-01 00:00:00
abstract::We studied the psychophysiology of soluble intercellular adhesion molecule-1 (sICAM-1) in 25 apparently healthy middle-aged men who underwent an acute psychosocial stressor three times with one week apart. Measures of the biological stress response were obtained at week one and three. The magnitude of the sICAM-1 stre...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Statins are the most commonly used drugs in patients with dyslipidemia. Among the patients, a significant inter-individual variability with supposed strong genetic background in statin treatment efficacy has been observed. Genome wide screenings detected variants within the CELSR2/PSRC1/SORT1, CILP2/PBX4, APOB, APOE/C...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932341
更新日期:2012-01-01 00:00:00
abstract::Exposure to hyperbaric oxygen leads to increased amount of reactive oxygen species (ROS) that are derived from various sources. After the discovery that ROS can function as signaling molecules, the idea of ROS being hazardous to biological tissues has been challenged. The aim of this study was to examine the changes i...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Epitestosterone competes with testosterone for androgen receptors and inhibits several enzymes of steroidogenesis. Insulin-like growth factors (IGFs) stimulate the growth of prostate cells and directly activate androgen receptors in prostatic tumor cell lines. The prostate-specific antigen (PSA) decreases the affinity...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Continuous normobaric hypoxia (CNH) renders the heart more tolerant to acute ischemia/reperfusion injury. Protein kinase C (PKC) is an important component of the protective signaling pathway, but the contribution of individual PKC isoforms under different hypoxic conditions is poorly understood. The aim of this study ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932860
更新日期:2015-01-01 00:00:00
abstract::Certain liver metabolic diseases point to the presence of disturbances in glycogen deposition. Epinephrine raises the cAMP level that activates protein kinase A leading to the activation of phosphorylase and glycogen breakdown. In the present report, we sought to investigate whether NO is produced during adrenoceptor ...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::We investigated the effects of telmisartan, the blocker of angiotensin II receptor 1, on the regulation of systolic blood pressure (SBP) and oxidative stress through endothelial nitric oxide (NO) release in spontaneously hypertensive rats (SHRs). SHRs randomly received placebo, oral feeding of telmisartan (5 mg/kg or ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932433
更新日期:2013-01-01 00:00:00
abstract::Pulse dye densitometry (PDD) enables the evaluation of hemodynamic state as well as liver function. A repeated examination, even after a short pause (or under stress condition), enables to follow safely the dynamics of liver pathology. From presented parameters we have evaluated as reliable the C5-clearance, an expres...
journal_title:Physiological research
pub_type: 临床试验,杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Wire myograph is a device for the in vitro investigation of both, active and passive properties of arteries. Arteries from a variety of animal species, pathological states, and vascular beds were investigated using this method. We focus on the normalization procedure which is aimed to standardize experimental settings...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::Electrophysiological studies of whole organ systems in vitro often require measurement of nerve activity and/or stimulation of the organ via the associated nerves. Currently two-compartment setups are used for such studies. These setups are complicated and require two fluids in two separate compartments and stretching...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933099
更新日期:2016-06-20 00:00:00
abstract::Our previous studies concerning the role of furosemide-resistant cation leaks in genetic hypertension demonstrated that blood pressure of recombinant inbred strains (derived from F2 hybrids of spontaneously hypertensive and normotensive Brown Norway rats) cosegregated with inward Na+ leak (determined in saline medium)...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00