Image analysis of argyrophil nucleolar organizer regions (AgNORs) in oestrogen-induced rat anterior pituitary hyperplasia: comparison of automatized (LUCIA M) and non-automatized evaluation.

Abstract:

:Argyrophil nucleolar organizer regions (AgNORs) were morphometrically evaluated in rat anterior pituitaries of control and oestrogenized rats using an automatized (LUCIA M--Laboratory Universal Computer Image Analysis Micro) and a non-automatized approaches to investigate not only the sensitivity and effectiveness of the automatized procedure but also the morphology of AgNORs in oestrogen-induced rat anterior pituitary hyperplasia. In the experimental oestrogen-induced rat anterior pituitary hyperplasia, the image analysis system LUCIA M was compared to a non-automatized morphometric procedure and proved to be very efficient and yielded analogical results. The AgNOR morphology in the oestrogenized pituitaries was characterized by an increase of the total AgNOR area and clustering of AgNORs in the large nucleoli.

journal_name

Physiol Res

journal_title

Physiological research

authors

Dusková J,Povýsil C,Schreiber V

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

217-25

issue

4

eissn

0862-8408

issn

1802-9973

journal_volume

44

pub_type

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