Abstract：
:Endometrial cancer is one of the most frequent gynecological malignancies present in more than 95 % of all uterine cancers. In spite of that, screening of such disease is not commonly performed in clinical practice due to enormous costs and relatively low sensitivity. Therefore, developing an effective screening test to diagnose endometrial cancer at early stages is of great importance for the clinical area of investigation. In this work, we applied urinary proteomics (i.e., bottom-up proteomic approach followed by nano HPLC-ESI-MS/MS) in patients with endometrial cancer, with respect to find proteins aimed for the early diagnostics and screening. According to the results, the significant semi-quantitative changes were observed in urinary proteome of treated patients. The proteins that may be pivotal in pathogenesis of endometrial cancer, like cadherin-1 (CDH1), vitronectin (VTN) and basement membrane specific-heparan sulphate proteoglycan core protein (HSPG2) were down-regulated, when compared to the control group. Ultimately, it can be stated that urinary proteomics has a potential for the searching of cancer protein biomarkers based on their altered concentration.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Kacírová M,Bober P,Alexovič M,Tomková Z,Tkáčiková S,Talian I,Mederová L,Bérešová D,Tóth R,Andrašina I,Kožlejová Z,Kilík R,Divín R,Sabo Jdoi
10.33549/physiolres.934375subject
Has Abstractpub_date
2019-12-30 00:00:00pages
S483-S490issue
Suppl 4eissn
0862-8408issn
1802-9973pii
934375journal_volume
68pub_type
杂志文章abstract：:It was recognized that recombinant inbred strains are a very powerful system for the study of the genetics of hypertension, linkage analysis and gene mapping. Such set of recombinant inbred strains has been developed in the cooperation of Prof V. Kren and Dr. M. Pravenec in Prague. These recombinant inbred strains wer...
journal_title：Physiological research
pub_type： 社论
doi：
更新日期：1993-01-01 00:00:00
abstract：:We determined and characterized the Mg2+-dependent, Ca2+-stimulated ATPase (Ca-ATPase) activity in cell plasma membranes from the myometrium of pregnant women, and compared these characteristics to those of the active Ca2+-transport already demonstrated in this tissue. Similarly to the Ca2+-transport system, the Ca2+-...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Pulse dye densitometry (PDD) enables the evaluation of hemodynamic state as well as liver function. A repeated examination, even after a short pause (or under stress condition), enables to follow safely the dynamics of liver pathology. From presented parameters we have evaluated as reliable the C5-clearance, an expres...
journal_title：Physiological research
pub_type： 临床试验,杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Expression of the immediate-early gene c-fos, a marker of neuronal activation was employed in adult anesthetized non-decerebrate cats, in order to localize the brainstem neuronal populations functionally related to sniff-like (gasp-like) aspiration reflex (AR). Tissues were immunoprocessed using an antibody raised aga...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:During 48 hours after application sublethal doses of hepatotoxic agent thiacetamide decrease cytochrome c oxidase activity of rat liver homogenate and isolated mitochondria to 46% and 32% of original values, respectively. This decrease may be prevented by simultaneous application of lipid emulsion (mixture of Lipofund...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:It has been reported that the major function of the sterol regulatory element binding protein 2 (SREBP-2) is to activate preferentially cholesterol biosynthesis in liver and adipose tissue rather than fatty acid synthesis. In the current study, we analyzed the effects of overexpression of human dominant-positive SREBP...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932751
更新日期：2014-01-01 00:00:00
abstract：:Brain imaging studies suggest localization of verbal working memory in the left dorsolateral prefrontal cortex (DLPFC) while face processing and memory is localized in the inferior temporal cortex and other brain areas. The goal of this study was to assess the effect of left DLPFC low-frequency repetitive transcranial...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:In this work, we evaluated the effect of adaptation to heat on the fall of blood pressure (BP) induced by heat shock (HS) and the interrelation between nitric oxide (NO) and heat shock protein, HSP70. Experiments were carried out on Wistar rats. It was shown that HS resulted in a generalized and transient increase in ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Hypolipidemic and cardioprotective effects of statins can be associated with the development of myopathies and new-onset type 2 diabetes. These adverse effects may be related to increased oxidative stress. The plant extract silymarin (SM) is known for its antioxidant and anti-inflammatory actions. We tested the hypoth...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2021-01-14 00:00:00
abstract：:The role of [Ca2+]i and cAMP in transduction of the melatonin inhibitory effect on GnRH-induced LH release from neonatal rat gonadotrophs has been studied, because melatonin inhibits the increase of both intracellular messengers. Treatments increasing Ca2+ influx (S(-) Bay K8644 or KCI) or cAMP concentration (8-bromo-...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933866
更新日期：2018-12-18 00:00:00
abstract：:Metabolic syndrome and one of its manifestations, essential hypertension, is an important cause of worldwide morbidity and mortality. Morbidity and mortality associated with hypertension are caused by organ complications. Previously we revealed a decrease of blood pressure and an amelioration of cardiac fibrosis in a ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934127
更新日期：2019-10-25 00:00:00
abstract：:The aim of this study was to assess the molecular basis of renal Na,K-ATPase disturbances in response to NO-deficient hypertension induced in rats by NO-synthase inhibition with 40 mg/kg/day N(G)-nitro-L-arginine methyl ester (L-NAME) for four weeks. After 4-week administration of L-NAME, the systolic blood pressure (...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Primary aldosteronism (PA) is the most common cause of endocrine hypertension with a high frequency of cardiovascular complications. The unfavorable cardiometabolic profile may be due to aldosterone-mediated activation of inflammatory cells, circulatory cytokines and activation of collagen synthesis in the vessel wall...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932994
更新日期：2016-06-20 00:00:00
abstract：:Kainic acid (KA) is a potent neurotoxic substance valuable in research of temporal lobe epilepsy. We tested how subconvulsive dose of KA influences spontaneous behavior of adult Wistar rats. Animals were treated with 5 mg/kg of KA and tested in Laboras open field test for one hour in order to evaluate various behavior...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932793
更新日期：2015-01-01 00:00:00
abstract：:Aquaporin proteins (AQPs) are a family of channels expressed in numerous mammalian tissues, where they play a fundamental role in regulating water transport across cell membranes. Based on reports that AQPs are present in the reproductive system and participate in reproductive processes, our aim was to investigate the...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933095
更新日期：2016-11-08 00:00:00
abstract：:Hyperuricemia has been described as associated with the risk of development metabolic syndrome; however the relationship between the uric acid level and particular parameters of metabolic syndrome remained unclear. We performed a cross-sectional study on a cohort of 833 dyslipidemic patients and correlated their level...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933410
更新日期：2017-07-18 00:00:00
abstract：:Progressive compromise of antioxidant defenses and free radical-mediated lipid peroxidation, which is one of the major mechanisms of secondary traumatic brain injury (TBI), has also been reported in pediatric head trauma. In the present study, we aimed to demonstrate the effect of melatonin, which is a potent free rad...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:The basis for most acute coronary events is either rupture or fissuring of unstable atherosclerotic plaques with subsequent thrombosis leading to coronary artery occlusion. The development of atherosclerotic plaques takes several decades, but the mechanical features determining its stability and the risk of rupture ca...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
abstract：:A new concept of cardioprotection based on the exploitation of endogenous mechanisms is known as ischemic preconditioning (IPC). It has been hypothesized that substances released during brief ischemic stress (e.g. catecholamines) stimulate the receptors and trigger multiple cell signaling cascades. Opening of ATP-sens...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Young intact (18 days of age) and adult ovariectomized (OV-X, ovariectomized between 21 to 24 days of age) C3H/Di mice were used to measure the estrogenicity on the basis of the growth response of mammary epithelial structures and weight of the uterus. The percentage area of the mammary fat pad occupied by mammary epi...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Continuous normobaric hypoxia (CNH) renders the heart more tolerant to acute ischemia/reperfusion injury. Protein kinase C (PKC) is an important component of the protective signaling pathway, but the contribution of individual PKC isoforms under different hypoxic conditions is poorly understood. The aim of this study ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932860
更新日期：2015-01-01 00:00:00
abstract：:The peroxisome proliferator-activated receptors (PPAR) belong to the nuclear superfamily of ligand-activated transcription factors. PPARgamma acts as a nutrient sensor that regulates several homeostatic functions. Its disruption can lead to vascular pathologies, disorders of fatty acid/lipid metabolism and insulin res...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.933439
更新日期：2016-10-24 00:00:00
abstract：:The main role of research in medicine is to provide relevant knowledge which, after successful translation to clinical practice, improves the quality of healthcare. The sex bias which is still present in the majority of research disciplines prefers male subjects despite legislation changes in the US grant agencies and...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934593
更新日期：2020-12-31 00:00:00
abstract：:Heart failure has become the most widely studied syndrome in cardiology over the recent years. Despite the encouraging achievements by angiotensin converting enzyme (ACE) inhibitors, the mortality of patients with chronic heart failure remains high. There are several factors which can potentially be responsible for th...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：1999-01-01 00:00:00
abstract：:The incidence of metabolic syndrome increases in the developed countries, therefore biomedical research is focused on the understanding of its etiology. The study of exact mechanisms is very complicated because both genetic and environmental factors contribute to this complex disease. The ability of environmental fact...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.933174
更新日期：2015-01-01 00:00:00
abstract：:The purpose of this study was to investigate plasma concentrations of cyclic guanosine monophosphate (cGMP) and atrial natriuretic peptide (ANP) during and after real and simulated space flight. Venous blood was obtained 3 min after the beginning and 2 min after the lower body negative pressure maneuver in two cosmona...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Our previous studies concerning the role of furosemide-resistant cation leaks in genetic hypertension demonstrated that blood pressure of recombinant inbred strains (derived from F2 hybrids of spontaneously hypertensive and normotensive Brown Norway rats) cosegregated with inward Na+ leak (determined in saline medium)...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Male rats aged 45, 85, 145 and 270 days (daily body mass increments on an optimal diet containing casein were 6.73, 2.88, 0.53 and 0.31 g respectively) were fed 15 days ad libitum on a diet with a nutrient content physiological for their age, in which the protein source was milk casein (ratio of essential to nonessent...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Erythrocytes (RBC) from untrained male Wistar rats and rat glomerular endothelial cells (EC) were used to investigate the effects of acute exercise (speed: 20 m/min, slope: 0, duration: 1 hour) on RBC membrane protein oxidation and adhesion to cultured EC. Experimental animals were divided into juvenile (age 10 weeks)...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
