Allosteric activation of yeast enzyme neutral trehalase by calcium and 14-3-3 protein.

Abstract:

:Neutral trehalase 1 (Nth1) from Saccharomyces cerevisiae catalyzes disaccharide trehalose hydrolysis and helps yeast to survive adverse conditions, such as heat shock, starvation or oxidative stress. 14-3-3 proteins, master regulators of hundreds of partner proteins, participate in many key cellular processes. Nth1 is activated by phosphorylation followed by 14-3-3 protein (Bmh) binding. The activation mechanism is also potentiated by Ca(2+) binding within the EF-hand-like motif. This review summarizes the current knowledge about trehalases and the molecular and structural basis of Nth1 activation. The crystal structure of fully active Nth1 bound to 14-3-3 protein provided the first high-resolution view of a trehalase from a eukaryotic organism and showed 14-3-3 proteins as structural modulators and allosteric effectors of multi-domain binding partners.

journal_name

Physiol Res

journal_title

Physiological research

authors

Alblova M,Smidova A,Kalabova D,Lentini Santo D,Obsil T,Obsilova V

doi

10.33549/physiolres.933950

subject

Has Abstract

pub_date

2019-04-30 00:00:00

pages

147-160

issue

2

eissn

0862-8408

issn

1802-9973

pii

933950

journal_volume

68

pub_type

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