Abstract:
:Atrial fibrillation is associated with atrial remodeling, in which connexin 43 (Cx43) and cell hypertrophy play important roles. In this study, apelin-13, an aliphatic peptide, was used to explore the protective effects of the adenosine monophosphate-activated protein kinase (AMPK)/mTOR signaling pathway on Cx43 expression and autophagy, using murine atrial HL-1 cells. The expression of Cx43, AMPK, B-type natriuretic peptide (BNP) and pathway-related proteins was detected by Western blot analysis. Cellular fluorescence imaging was used to visualize Cx43 distribution and the cytoskeleton. Our results showed that the Cx43 expression was significantly decreased in HL-1 cells treated with angiotensin II but increased in cells additionally treated with apelin-13. Meanwhile, apelin-13 decreased BNP expression and increased AMPK expression. However, the expression of Cx43 and LC3 increased by apelin-13 was inhibited by treatment with compound C, an AMPK inhibitor. In addition, rapamycin, an mTOR inhibitor, promoted the development of autophagy, further inhibited the protective effect on Cx43 expression and increased cell hypertrophy. Thus, apelin-13 enhances Cx43 expression and autophagy via the AMPK/mTOR signaling pathway, and serving as a potential therapeutic target for atrial fibrillation.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Chen Y,Qiao X,Zhang L,Li X,Liu Qdoi
10.33549/physiolres.934488subject
Has Abstractpub_date
2020-11-16 00:00:00pages
813-822issue
5eissn
0862-8408issn
1802-9973pii
934488journal_volume
69pub_type
杂志文章abstract::Antagonists of GABA(B) receptors are expected to have proconvulsant action also in developing brain. Two antagonists (CGP55845 and CGP46381) were tested in a model of cortical epileptic afterdischarges (ADs) in 12-, 18- and 25-day-old rat pups with implanted electrodes. CGP55845 was dissolved in dimethylsulfoxide and ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932599
更新日期:2013-01-01 00:00:00
abstract::We investigated how postural responses to galvanic vestibular stimulation were affected by standing on a translating support surface and by somatosensory loss due to diabetic neuropathy. We tested the hypothesis that an unstable surface and somatosensory loss can result in an increase of vestibulospinal sensitivity. B...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933866
更新日期:2018-12-18 00:00:00
abstract::We analyzed the effect of FK 506 on the production of nitric oxide by macrophages. Isolated rat peritoneal macrophages were cultured for 24 h with or without lipopolysaccharide (LPS) (5 microg/ml) and in the absence or presence of FK 506 (0.1 and 1 microg/ml). The concentration of NO2- in culture supernatants was take...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The objective of this study was to evaluate the effect of diet and 677 C-->T mutation of the methylenetetrahydrofolate reductase (MTHFR) gene on plasma homocyst(e)ine concentrations in an adolescent population (113 males, age: 14.2+/-2.4 years; 202 females, age: 14.9+/-2.1 years) from a region characterized by high ca...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::MiRNAs are important regulators of gene expression and changes in their levels are linked with various pathological states, including solid tumors. MiR-215 has been identified as a tumor suppressor in colorectal cancer (CRC). Following our previous in vitro and in vivo experiments, the aim of this project was to study...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933546
更新日期:2016-12-21 00:00:00
abstract::Leptin and adiponectin, two adipocytokines, may work together in regulating energy homeostasis and insulin action. Leptin gene expression has been investigated in term placental tissue complicated by gestational diabetes mellitus (GDM), but never in conjunction with all isoforms of the leptin receptor (LEPR A-D), or w...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Circadian clock plays an essential role in orchestrating daily physiology, and its disruption can evoke metabolic diseases such as obesity. L-Carnitine can reduce blood lipid levels, and ameliorate fatty liver through regulating lipid metabolism. However, whether L-Carnitine administration may affect the disturbance o...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933543
更新日期:2017-11-24 00:00:00
abstract::Vascular endothelial growth factor (VEGF) has an important role in the pathogenesis of retinopathy of prematurity (ROP) and inhibition of VEGF expression in the neovascular phase might prevent destructive neovascularization in ROP. It is suggested that retinoids exert a highly potent antiangiogenic activity by inhibit...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::In this study we compared several parameters characterizing differences in the lipoprotein profile between members of families with a positive or negative family history of coronary artery disease (CAD). In addition to regular parameters such as the body mass index (BMI), total plasma cholesterol (TC), low density (LD...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Instead of a comprehensive review, we describe the basic undisputed facts and a modest contribution of our group to the fascinating area of the research on mitochondrial uncoupling proteins. After defining the terms uncoupling, leak, protein-mediated uncoupling, we discuss the assumption that due to their low abundanc...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:
更新日期:2004-01-01 00:00:00
abstract::Aquaporin proteins (AQPs) are a family of channels expressed in numerous mammalian tissues, where they play a fundamental role in regulating water transport across cell membranes. Based on reports that AQPs are present in the reproductive system and participate in reproductive processes, our aim was to investigate the...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933095
更新日期:2016-11-08 00:00:00
abstract::Growth of the A549 cell line in a perfusion system suitable for use in a magnetic resonance study has been characterized and shown to be stable physiologically and hence appropriate for serial observations. Several methods of monitoring cell growth were compared to assess the behavior of the cells in this system. Comp...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Metabolic syndrome and one of its manifestations, essential hypertension, is an important cause of worldwide morbidity and mortality. Morbidity and mortality associated with hypertension are caused by organ complications. Previously we revealed a decrease of blood pressure and an amelioration of cardiac fibrosis in a ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.934127
更新日期:2019-10-25 00:00:00
abstract::Photodynamic therapy (PDT) is now being used more frequently in carefully selected cases of malignancies. The drugs used for PDT are mostly derivatives of haematoporphyrine (HPD) and its active component photofrine II. Another compound prepared by total synthesis is meso-tetra-(4-sulfonatophenyl)-porphine (TPPS4) but ...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Hypoxic pulmonary vasoconstriction (HPV) occurs in smooth muscle cells (SMC) from small pulmonary arteries (SPA) and is accompanied by increases in free cytoplasmic calcium ([Ca2+]i) and cytoplasmic pH (pHi). SMC from large pulmonary arteries (LPA) relax during hypoxia, and [Ca2+]i and pHi decrease. Increases in pHi a...
journal_title:Physiological research
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::The effects of selenium (Se) on antioxidant defense system in liver and kidneys of rats with cadmium (Cd)-induced toxicity were examined. Cd exposure (15 mg Cd/kg b.m./day as CdCl(2) for 4 weeks) resulted in increased lipid peroxidation (LP) in both organs (p<0.005 and p<0.01). Vitamin C (Vit C) was decreased in the l...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The role of the cortico-tectal pathways in the processing of auditory signals was investigated by recording the click-evoked responses and extracellular multiple unit activity in the inferior colliculus (IC) after functional ablation of the auditory cortex (AC) by local intracortical application of a sodium channel bl...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The aim of this study was to assess the molecular basis of renal Na,K-ATPase disturbances in response to NO-deficient hypertension induced in rats by NO-synthase inhibition with 40 mg/kg/day N(G)-nitro-L-arginine methyl ester (L-NAME) for four weeks. After 4-week administration of L-NAME, the systolic blood pressure (...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Numerous countermeasures have been proposed to minimize microgravity-induced physical deconditioning, but their benefits are limited. The present study aimed to investigate whether personalized aerobic exercise based on artificial gravity (AG) mitigates multisystem physical deconditioning. Fourteen men were assigned t...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.933493
更新日期:2017-09-22 00:00:00
abstract::The lymphatic bioavailability (FL) of diazepam (DZ) and its major metabolite desmethyldiazepam (DDZ) was studied. DZ was administered in intravenous and intraduodenal boluses, and in intravenous infusion in three groups of rats with different total lipid (TL) content in the central lymph. The effect of a) different li...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Brain imaging studies suggest localization of verbal working memory in the left dorsolateral prefrontal cortex (DLPFC) while face processing and memory is localized in the inferior temporal cortex and other brain areas. The goal of this study was to assess the effect of left DLPFC low-frequency repetitive transcranial...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::This study was undertaken to investigate the effects of lower body positive pressure (LBPP) on cardiovascular responses during a 15-min walking trial in young (22.1+/-0.4 years) and elderly women (67.8+/-1.1 years). The application of 20 mm Hg LBPP reduced ground reaction forces by 31.2+/-0.5 kgw in both groups. We hy...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932459
更新日期:2013-01-01 00:00:00
abstract::The potential of novel scaffold containing sodium hyaluronate, type I collagen, and fibrin was investigated in the regeneration of osteochondral defects in miniature pigs. Both autologous chondrocyte-seeded scaffolds and non-seeded scaffolds were implanted into two defects located in the non-weight-bearing zone of the...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Methamphetamine (MA), as a psychostimulant drug that crosses the placental barrier, may disrupt the development of social play. The present study aims to examine the effect of prenatal MA (5 mg/kg) exposure during the first (gestational day (GD) 1-11) or second (GD 12-22) halves of prenatal development of rats on soci...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.934230
更新日期:2020-04-30 00:00:00
abstract::The concept of vena contracta space reduction in tricuspid valve position was tested in an animal model. Feasibility of specific artificial obturator body (REMOT) fixed to the right ventricular apex and interacting with tricuspid valve leaflets was evaluated in three different animal studies. Catheter-based technique ...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932580
更新日期:2014-01-01 00:00:00
abstract::Body surface potential maps have and certainly will have a very important role in the field of clinical arrhythmology, specifically for the localization of accessory pathways, for the detection of the origin of ventricular arrhythmias and for the identification of patients at risk of sudden death. In this particular s...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::We performed a systematic study to check whether neurons in the area TE (the anterior part of inferotemporal cortex) in rhesus monkey, regarded as the last stage of the ventral visual pathway, could be modulated by auditory stimuli. Two fixating rhesus monkeys were presented with visual, auditory or combined audiovisu...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932172
更新日期:2011-01-01 00:00:00
abstract::We have investigated the effect of a diet containing of 4% oyster fungus (Pleurotus ostreatus) and 0.1% cholesterol on glycaemia and hyperlipoproteinaemia in rats with insulin-dependent diabetes (streptozotocin 45 mg/kg). After two months, the rats with diabetes kept on the oyster fungus diet, had a significantly lowe...
journal_title:Physiological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::The effects of inclusion of sesamin / episesamin in Baltic Atlantic salmon (Salmo salar L.) diets based on vegetable oils were studied. The study was designed as a dose response study with two control diets, one diet based on fish oil (FO) and one diet based on a mixture of linseed and sunflower oil (6:4 by vol.) (MO)...
journal_title:Physiological research
pub_type: 杂志文章
doi:10.33549/physiolres.932068
更新日期:2011-01-01 00:00:00