Abstract：
:We analyzed the effect of FK 506 on the production of nitric oxide by macrophages. Isolated rat peritoneal macrophages were cultured for 24 h with or without lipopolysaccharide (LPS) (5 microg/ml) and in the absence or presence of FK 506 (0.1 and 1 microg/ml). The concentration of NO2- in culture supernatants was taken as a measure of nitric oxide production. FK 506 (0.1 and 1 microg/ml) reduced the LPS-induced increase of NO2- levels by 68% and 81%, respectively. The impact of cyclosporin A (CsA) was studied in order to compare their effects. CsA (0.1 and 1 microg/ml) decreased the levels of nitrites by 39% and 69%, respectively. The results obtained suggest that both immunosuppressive drugs exhibit a dose-dependent inhibitory effect on nitric oxide production and that FK 506 is a more potent agent than CsA in this respect.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Strestíková P,Otová B,Filipec M,Farghali Hsubject
Has Abstractpub_date
2000-01-01 00:00:00pages
725-8issue
6eissn
0862-8408issn
1802-9973journal_volume
49pub_type
杂志文章abstract：:The pathological potential of glial cells was recognized already by Rudolf Virchow, Santiago Ramon y Cajal and Pio Del Rio-Ortega. Many functions and roles performed by astroglia in the healthy brain determine their involvement in brain diseases; as indeed any kind of brain insult does affect astrocytes, and their per...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：2008-01-01 00:00:00
abstract：:It is believed that atherogenesis is a multifactorial process, which could already start in utero. Development of atherosclerosis progresses over decades and leads to the cardiovascular morbidity and mortality in adulthood. At present, we have no exact explanation for all the risk factors acting in the pathogenesis of...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Mammalian Meckel´s cartilage is a temporary structure associated with mandible development. Notably, its elimination is not executed by apoptosis, and autophagy was suggested as the major mechanism. Simultaneous reports point to pro-apoptotic caspases as novel participants in autophagic pathways in general. The aim of...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933947
更新日期：2019-03-06 00:00:00
abstract：:The present study deals with the effect of hypergravity (2xg) on the pH and on the proteolytic activity in the digesta of the gastrointestinal tract of Japanese quails during intense growth. The birds were raised on a semisynthetic diet containing free amino acids (A) and a commercial diet (B). During days 35 till 40 ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:In this work, we evaluated the effect of adaptation to heat on the fall of blood pressure (BP) induced by heat shock (HS) and the interrelation between nitric oxide (NO) and heat shock protein, HSP70. Experiments were carried out on Wistar rats. It was shown that HS resulted in a generalized and transient increase in ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:The main role of research in medicine is to provide relevant knowledge which, after successful translation to clinical practice, improves the quality of healthcare. The sex bias which is still present in the majority of research disciplines prefers male subjects despite legislation changes in the US grant agencies and...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934593
更新日期：2020-12-31 00:00:00
abstract：:A new approach to computer modelling of neuronal stochastic activity is described. The output dynamic activity which depends on the types and the number of input synapses, weights of the synaptic efficacy, the absolute refractory phase duration and threshold level is evaluated on this model in some types of Gaussian i...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Male and female rats were given oestradiol benzoate (1 mg s.c. twice a week for 3 weeks) and/or sodium nitroprusside (SN), a donor of nitric oxide (NO), which was administered in their food in amounts of 0.2 or 0.6 mg/rat/day. Neither oestradiol-induced hypertrophy of the hypophysis, nor the serum prolactin (PRL) leve...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:A "pulse like" increase of cytoplasmic calcium concentration, which is proportional to ionophore concentration, is induced in red cells by exposure to A23187. Different Ca2+ levels are attained depending on cellular calcium buffering power and/or primary active calcium transport activation. We examined the effect of A...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Stomach lesions induced by indomethacin (20 mg.kg-1 i.p.) and ethanol (1 ml 95% intragastrically) were studied after a 24 hour fast in rats which had undergone sialoadenectomy. The size of the lesions was correlated with gastric HCl secretion, with gastric vascular permeability (determined from the Evans blue concentr...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Progesterone and estradiol are the foremost steroid hormones in human pregnancy. However, the origin of maternal progesterone has still not been satisfactorily explained, despite the generally accepted opinion that maternal LDL-cholesterol is a single substrate for placental synthesis of maternal progesterone. The que...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.931807
更新日期：2010-01-01 00:00:00
abstract：:Statins are the most commonly used drugs in patients with dyslipidemia. Among the patients, a significant inter-individual variability with supposed strong genetic background in statin treatment efficacy has been observed. Genome wide screenings detected variants within the CELSR2/PSRC1/SORT1, CILP2/PBX4, APOB, APOE/C...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932341
更新日期：2012-01-01 00:00:00
abstract：:Growth of the A549 cell line in a perfusion system suitable for use in a magnetic resonance study has been characterized and shown to be stable physiologically and hence appropriate for serial observations. Several methods of monitoring cell growth were compared to assess the behavior of the cells in this system. Comp...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Pulse dye densitometry (PDD) enables the evaluation of hemodynamic state as well as liver function. A repeated examination, even after a short pause (or under stress condition), enables to follow safely the dynamics of liver pathology. From presented parameters we have evaluated as reliable the C5-clearance, an expres...
journal_title：Physiological research
pub_type： 临床试验,杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Atherogenesis involves the migration of leukocytes into vascular subendothelial space, a process mediated by endothelial and leukocyte cell adhesion molecules. Endothelial molecules are assessed indirectly via serum levels, but leukocyte molecules can be assessed directly. We have therefore hypothesized that leukocyte...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Hyperbaric oxygen (HBO) therapy, i.e. breathing pure oxygen under increased environmental pressures serves as a treatment for diverse medical conditions. However, elevated oxygen concentration can be detrimental to central nervous system or lungs. Our study aimed to evaluate the effects of repeated exposure to HBO on ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934046
更新日期：2018-12-31 00:00:00
abstract：:The goal of this study was to identify small molecular weight compounds that bind to sclerostin using in-silico methods because of the established importance of sclerostin-based therapies for the treatment of disease characterized by low bone mass. The zinc database (Zdb) revealed that nine potential molecules bind to...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933267
更新日期：2016-11-23 00:00:00
abstract：:Hypericin is a photosensitizing plant pigment from Hypericum perforatum with multiple modes of light-induced biological activities due to production of singlet oxygen and/or excited-state proton transfer with consequent pH drop in the hypericin environment. In the present work, we studied the effects of three inhibito...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Dihydrotestosterone (DHT) originates via irreversible reduction of testosterone by catalytic activity of 5alpha-reductase enzyme and it is demonstratively the most effective androgen. Androgens influence adipose tissue in men either directly by stimulation of the androgen receptor or indirectly, after aromatization, b...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.932080
更新日期：2011-01-01 00:00:00
abstract：:To evaluate the role of chloride in the pathogenesis of salt-dependent deoxycorticosterone (DOC) hypertension, we studied young Wistar rats chronically loaded with sodium bicarbonate (NaHCO(3)) or sodium chloride (NaCl) which were administered either in the diet or in the drinking fluid. Selective sodium loading (with...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Through the analysis of the common apolipoprotein (apo) E gene polymorphism in large Caucasian population study with the PCR and subsequent restriction analysis, we have identified carriers of mutant allele Arg136-->Ser. Both of them (71-years-old female and her 43-years-old son) have normal lipid parameters. We sugge...
journal_title：Physiological research
pub_type： 临床试验,杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Fever developing after intracerebral injections of lipopolysaccharide (LPS) to guinea-pigs were monophasic, with only one peak of inner body temperature slowly developing and longlasting in a dose range 20 to 200 ng of LPS. Latency time was inversely related to the dose of LPS. Indomethacin injected to the third brain...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:The paper presents an example of a new type of a structured model containing time delays in parallel branches. This model was selected as optimal to describe mathematically the lymphocyte migration between the venous blood and prescapular lymph in Merino ewes under physiological conditions. The model allowed to identi...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Differential pulse voltammetry with a carbon fibre microelectrode (ME) was used in pentobarbital-anaesthetized rats for monitoring the stobadine current (STB.C) on both sides of the blood-brain barrier (BBB) in the arterial bloodstream (BS) and in the corpus striatum (CS). The STB.C exhibited a distinct peak at a pola...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Hindlimb unweighting (HU) leads to capillary regression in skeletal muscle. However, the molecular mechanism(s) remains to be elucidated. To gain insight into the regulation of this process, we investigated gene expression of hypoxia-inducible factor-1alpha (HIF-1alpha)vascular endothelial growth factor (VEGF), angiop...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:The potential of novel scaffold containing sodium hyaluronate, type I collagen, and fibrin was investigated in the regeneration of osteochondral defects in miniature pigs. Both autologous chondrocyte-seeded scaffolds and non-seeded scaffolds were implanted into two defects located in the non-weight-bearing zone of the...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:In this study we analyzed the effects of melatonin (Mel, 1 mg/kg ip) on behavioral changes as well as cell and oxidative damage prompted by bilaterally olfactory bulbectomy. Olfactory bulbectomy caused an increase in lipid peroxidation products and caspase-3, whereas it prompted a decrease of reduced glutathione (GSH)...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.931684
更新日期：2010-01-01 00:00:00
abstract：:Endothelin-1 (ET-1) acts on ET(A) and ET(B) receptors and has been implicated in hemorrhagic shock (shock). We determined effect of shock and resuscitation by hypertonic saline (saline) or centhaquin on ET(A) and ET(B) receptor expression. Rats were anesthetized, a pressure catheter was placed in the left femoral arte...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933856
更新日期：2018-06-27 00:00:00
abstract：:Reduced physical activity and abundant energy intake are two most common factors leading to uncontrolled body weight gain. But these factors are not under entire internal consciousness control; they are also partially genetically determined and are affected by for example food marketing practices. In addition to these...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：2009-01-01 00:00:00
abstract：:Glucose tolerance, insulin binding to erythrocytes, insulinaemia, plasma total cholesterol, plasma triglycerides, weight of fat pads, food consumption and body weight changes were studied in genetically hypertensive lean Koletsky rats. Long-term treatment with dopaminergic agonist terguride (0.2 mg/kg/day) normalized ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
