Abstract:
:The immunogenicity of capsomeres of human papillomavirus type 33 was evaluated in a dose-response analysis. Capsomeres were obtained free of capsids by expression of L1 carrying the single point mutation C427S. Neutralizing antibodies were detected using an in vitro pseudoinfection assay. Capsomeres induced type-specific, neutralizing antibodies in mice even in the absence of adjuvant. The neutralization titers of immune sera raised without adjuvant were 10- to 20-fold lower than those of antisera to virus-like particles, but virtually identical using Freund's adjuvant. These data indicate that capsomeres may substitute for virus-like particles in future vaccines when used with an adjuvant appropriate for human vaccination.
journal_name
Virologyjournal_title
Virologyauthors
Fligge C,Giroglou T,Streeck RE,Sapp Mdoi
10.1006/viro.2000.0875subject
Has Abstractpub_date
2001-05-10 00:00:00pages
353-7issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(00)90875-4journal_volume
283pub_type
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