Abstract:
:We examined glomerular basement membrane anionic site distribution identified by cationic gold in seven patients with insulin-dependent and four patients with non-insulin-dependent diabetes mellitus, presenting a spectrum of clinical and glomerular changes. Anionic sites were investigated by pretreatment of tissue with glycosaminoglycan-degrading enzymes prior to cationic gold staining. The distribution of chondroitin sulphate proteoglycans--a previously unrecognized glomerular basement membrane component--and type IV collagen was examined by immunoelectron microscopy to identify structural changes in the basement membrane. Findings were compared with those of non-diabetic patients showing minor proteinuria and morphologically normal glomerular basement membranes. Two patients, originally diagnosed as having diabetic nephropathy were also examined at 19 weeks and 5 years after renal transplantation. Characteristic redistribution of type IV collagen and chondroitin sulphate proteoglycans was noted in thickened glomerular basement membrane segments (> 400 nm) of diabetic patients and those with renal transplants. Extension of anionic sites deep into the glomerular basement membrane at pH 2.5, together with loss of interna sites at pH 5.8 is unique to diabetic nephropathy. Reduced charge density was apparent in some patients due to thickening of the glomerular basement membrane, although the number of anionic sites per unit length of membrane was actually increased. Thus, charge aberration in diabetic nephropathy is due to displacement rather than loss of anionic sites. Removal of more than 90% of these sites by heparitinase, confirms their association with heparan sulphate proteoglycans. Similar derangement of anionic sites in all patients with diabetic nephropathy irrespective of the degree of proteinuria, suggests that a heparan sulphate proteoglycan-related charge barrier plays a minor role in controlling permeability of the diabetic glomerular basement membrane.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Goode NP,Shires M,Crellin DM,Aparicio SR,Davison AMdoi
10.1007/BF00400607subject
Has Abstractpub_date
1995-12-01 00:00:00pages
1455-65issue
12eissn
0012-186Xissn
1432-0428journal_volume
38pub_type
杂志文章相关文献
DIABETOLOGIA文献大全abstract:AIMS/HYPOTHESIS:Sphingolipid metabolism regulates beta cell biology and inflammation and is abnormal at the onset of type 1 diabetes. Fenofibrate, a regulator of sphingolipid metabolism, is known to prevent diabetes in NOD mice. Here, we aimed to investigate the effects of fenofibrate on the pancreatic lipidome, pancre...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-019-04973-z
更新日期:2019-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Low phosphate and high calcium concentrations have been linked to altered glucose tolerance and reduced insulin sensitivity in non-diabetic individuals. The aim of this study was to examine the relationships of calcium and phosphate levels and the calcium-phosphate product with the development of type 2...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-014-3241-9
更新日期:2014-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of this study was to evaluate the effects of oxygen consumption-matched short-term interval walking training (IWT) vs continuous walking training (CWT) on glycaemic control, including glycaemic variability, in individuals with type 2 diabetes. We also assessed whether any training-induced improv...
journal_title:Diabetologia
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00125-016-4170-6
更新日期:2017-03-01 00:00:00
abstract::Chronic diseases arising from obesity will continue to escalate over coming decades. Current approaches to combating obesity include lifestyle measures, surgical interventions and drugs that target weight reduction or the metabolic consequences of obesity. Lifestyle measures including physical activity are usually the...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s00125-009-1420-x
更新日期:2009-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:Cardiovascular events and death are better predicted by postprandial glucose (PPG) than by fasting blood glucose or HbA(1c). While chronic exercise reduces HbA(1c) in patients with type 2 diabetes, short-term exercise improves measures of insulin sensitivity but does not consistently alter responses to ...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章
doi:10.1007/s00125-012-2490-8
更新日期:2012-05-01 00:00:00
abstract::Aspects of the regulation of the glucose transport by perfused hearts of normal rats have been studied by measuring glucose transport (via the efflux of labelled 3-O-methyl-D-glucose) and glucose transporters (via the labelled cytochalasin B binding assay). Similarly to what is observed with insulin, increasing worklo...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00395557
更新日期:1988-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:We determined the response of selected genes to in vivo insulin in adipose tissue in 21 non-diabetic women. MATERIALS AND METHODS:The women were divided into insulin-sensitive and -resistant groups based on their median whole-body insulin sensitivity (8.7+/-0.4 vs 4.2+/-0.3 mg kg(-1) min(-1) for insuli...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-0075-5
更新日期:2006-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of this study was to determine whether presence of the metabolic syndrome in pregnancy associates with child telomere length or child anthropometry (weight, BMI) and BP, measured at 10 years of age. METHODS:The Screening for Pregnancy Endpoints study (SCOPE) was a multicentre, international pro...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-020-05242-0
更新日期:2020-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:Several investigations have shown that the renal medulla has a greater capacity to generate nitric oxide than the renal cortex. To further evaluate the changes of nitric oxide synthesis in the kidney, particularly in the outer medulla, in disorders involving fluid and electrolyte imbalances, we sought t...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051354
更新日期:2000-05-01 00:00:00
abstract:AIMS/HYPOTHESIS:A reduction in retinal blood flow occurs early in diabetes and is likely to be involved in the development of diabetic retinopathy. We hypothesise that activation of the arginase pathway could have a role in the vascular dysfunction of diabetic retinopathy. METHODS:Experiments were performed using a mo...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2789-5
更新日期:2013-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:Peroxisome proliferator-activated receptor (PPAR)-gamma coactivator-1 (PPARGC1), a coactivator regulating the transcription of genes involved in oxidative metabolism, is downregulated in patients with type 2 diabetes and in their first-degree relatives. Whether this downregulation is a cause or effect o...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0369-2
更新日期:2006-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:Abnormal rheological properties of erythrocytes, leucocytes and plasma may have a role in the development of diabetic microangiopathy. We hypothesized that changed haemorrheological variables may already be found in children with onset diabetes. METHODS:Erythrocyte deformation (rheoscope), neutrophil d...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051239
更新日期:1999-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:The effects of successful pancreas transplant alone (PTA) on chronic complications of diabetes, in particular diabetic retinopathy, remain disputed. We prospectively studied the course of diabetic retinopathy in PTA recipients and in non-transplanted (non-PTA) type 1 diabetic patients. METHODS:The PTA ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0463-5
更新日期:2006-12-01 00:00:00
abstract::Structural alterations in arterial extracellular matrix components have been suggested to play a role in the development of arterial disease among patients with diabetes mellitus. This study examines the quantity and quality of collagenous components in aortas from diabetic patients. In order to obtain data about the ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00402282
更新日期:1993-05-01 00:00:00
abstract::The purpose of this study was to investigate whether young insulin-dependent diabetic patients still develop peripheral nerve dysfunction when using modern multiple insulin injection therapy and to elucidate if this correlated with various disease parameters. Seventy-five patients, 7 to 20 years old with a duration of...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00401840
更新日期:1995-06-01 00:00:00
abstract::An incidence of 9.0 out of 100,000 children under 19 years of age with Type 1 (insulin-dependent) diabetes was detected in Toronto during a 2 year prospective study. An increased number of cases occurred in the winter months of one of the years but not of the other during the study. The annual incidence was the same i...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00281308
更新日期:1982-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:White cell count has been shown to predict incident type 2 diabetes, but differential white cell count has received scant attention. We examined the risk of developing diabetes associated with differential white cell count and neutrophil:lymphocyte ratio and the effect of insulin sensitivity and subclin...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-013-3080-0
更新日期:2014-01-01 00:00:00
abstract::The spontaneous hypertensive rat is an animal model characterized by a syndrome of hypertension, insulin resistance and hyperinsulinaemia. To elucidate whether in analogy to other insulin resistant animal models an inactivity of the insulin receptor kinase or an alteration of the glucose transporter (GLUT 4) level in ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00429089
更新日期:1992-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of this study was to determine the role of fatty acid signalling in islet beta cell compensation for insulin resistance in the Zucker fatty fa/fa (ZF) rat, a genetic model of severe obesity, hyperlipidaemia and insulin resistance that does not develop diabetes. MATERIALS AND METHODS:NEFA augmen...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0305-5
更新日期:2006-09-01 00:00:00
abstract::This paper presents a survey of the biological potencies of a variety of naturally-occurring and semi-synthetic insulin analogues and a study of the joint biological action of some of these materials with native insulin. Biological activity was tested on isolated rat fat cells using lipogenesis from glucose as the met...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF01219650
更新日期:1978-11-01 00:00:00
abstract::Recent studies have suggested an association between a deletion (D) variant of the angiotensin-converting-enzyme (ACE) gene and diabetic nephropathy. However, this finding has not been confirmed by all investigators. Furthermore, an M235T variant of the angiotensinogen (AGT) gene has been associated with hypertension,...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050662
更新日期:1997-02-01 00:00:00
abstract::In order to establish a model for the in vivo assessment of islet function we have used the Rowett nude rat with transplantation of allogeneic and xenogeneic (mouse) islets into the renal subcapsular space following a minimal period of diabetic induction. Thirty-one nude rats were given streptozotocin and 30 became di...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF02341509
更新日期:1988-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:This meta-analysis assessed the pooled effect of each genetic variant reproducibly associated with diabetic nephropathy. METHODS:PubMed, EMBASE and Web of Science were searched for articles assessing the association between genes and diabetic nephropathy. All genetic variants statistically associated w...
journal_title:Diabetologia
pub_type: 杂志文章,meta分析
doi:10.1007/s00125-010-1996-1
更新日期:2011-03-01 00:00:00
abstract::Mild hypoglycaemia was induced using an artificial pancreas in five normal subjects (from 5.00 +/- 0.15 to 2.83 +/- 0.15 mmol/l) by infusing 28 mU/m2 per min soluble insulin for 60 min. Six Type 1 (insulin-dependent) diabetic patients were stabilized for 14h using an artificial pancreas. They were then rendered hypogl...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00254837
更新日期:1982-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:The glomerular endothelial layer is coated by the endothelial surface layer (ESL), which is suggested to play a role in regulation of the permselectivity of macromolecules. Production of heparanase, a degrading enzyme of the ESL, is induced by reactive oxygen species (ROS). We hypothesised that oxidativ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-010-1810-0
更新日期:2010-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:Adiponectin is a circulating peptide derived from adipose tissue. It mediates its insulin-sensitising and anti-atherogenic effects on target tissues through two known receptors, adiponectin receptors 1 and 2 (ADIPOR1; ADIPOR2), which are encoded by the genes ADIPOR1 and ADIPOR2. Our aim was to study the...
journal_title:Diabetologia
pub_type: 杂志文章,多中心研究
doi:10.1007/s00125-006-0291-7
更新日期:2006-08-01 00:00:00
abstract::The recently completed diabetes complications and control trial has highlighted the need for improvement of insulin delivery systems for treatment of insulin-dependent diabetes mellitus. Despite steady improvement in methods for islet and whole pancreas transplantation over the past three decades, the broad-scale appl...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s001250051398
更新日期:1997-07-01 00:00:00
abstract::An autoimmune basis for the pathogenesis of insulin-dependent diabetes mellitus (IDDM) is supported by the frequent presence of autoantibodies - islet cell antibodies (ICAs) and GAD antibodies (GADab). However, in Chinese patients with clinical IDDM, a low prevalence of ICAs was observed. In non-insulin-dependent diab...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050845
更新日期:1997-12-01 00:00:00
abstract::We have developed a mouse monoclonal antibody against rat/mouse islet amyloid polypeptide (IAPP). The antibody recognises an epitope in the N-terminal part of the molecule, which is conserved between different species. The antibody immunohistochemically labelled beta cells in normal islets of most different mammalian ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050751
更新日期:1997-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:High levels of cardiorespiratory fitness (CRF) and physical activity (PA) are associated with a favourable metabolic risk profile. However, there has been no thorough exploration of the independent contributions of cardiorespiratory fitness and subcomponents of activity (total PA, time spent sedentary, ...
journal_title:Diabetologia
pub_type: 杂志文章,多中心研究
doi:10.1007/s00125-007-0762-5
更新日期:2007-09-01 00:00:00