Abstract：
:Proteolytic enzymes are believed to be necessary for tumor cell invasion. We have studied the effects of the cysteine proteinase inhibitor E-64 and the serine and cysteine proteinase inhibitor leupeptin, on the ability of human malignant melanoma cells (LOX) to pass through an artificial basement membrane. Transwell chambers containing filters coated with the reconstituted basement membrane, Matrigel, were used. Nontoxic concentrations of the proteinase inhibitors reduced the invasion of LOX cells through Matrigel. E-64 (250 mumol/l) by 27% and leupepetin (250 micrograms/ml) by 46%. The proteinase inhibitors did not alter the growth rate of the tumor cells, their motility through uncoated filters, or their attachment to the Matrigel coated wells. Our results indicate that cysteine proteinases are involved in the degradation of basement membranes and thus contribute to the invasion of malignant melanoma cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Bjørnland K,Buø L,Kjønniksen I,Larsen M,Fodstad O,Johansen HT,Aasen AOsubject
Has Abstractpub_date
1996-07-01 00:00:00pages
1627-31issue
4Aeissn
0250-7005issn
1791-7530journal_volume
16pub_type
杂志文章abstract：:Mutations affecting the mechanistic target of rapamycin (MTOR) signalling pathway are frequent in human cancer and have been identified in up to 15% of pancreatic neuroendocrine tumours (NETs). Grade A evidence supports the efficacy of MTOR inhibition with everolimus in pancreatic NETs. Although a significant proporti...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11367
更新日期：2017-02-01 00:00:00
abstract：:In the presence study we demonstrated that hycanthone and chlorophenoxamine can modulate the resistance of multidrug resistant (MDR) murine L1210 leukemia tumor lines in vitro and in vivo. The circumvention of MDR by hycanthone and chlorophenoxamine in vitro was demonstrated by a short-term test using tritiated nuclei...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:Human epidemiological studies suggest an association between N-acetyltransferase (NAT) activity and the incidence of bladder and colorectal cancers. In this study, paclitaxel was selected to examine the inhibition of arylamine NAT activity, gene expression and 2-aminofluorene-DNA adduct formation in a human osteogenic...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND/AIM:Rhabdoid tumors (RT) are aggressive pediatric tumors, which show poor prognosis despite use of multimodal intensive therapy. In these tumors, several different oncogenic pathways and epigenetic regulators (like CDK4/6-cyclinD-Rb-signaling, EZH2, histone deacetylases) are contemporaneously deregulated as ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
abstract：BACKGROUND/AIM:Primary Non-Hodgkin's lymphoma of the gallbladder (PNHL-GB) is extremely rare and data on clinical characteristics, optimal management and outcomes of these patients are limited to anecdotal reporting. We, therefore, sought to examine these patients using a population-based database. MATERIALS AND METHO...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11602
更新日期：2017-05-01 00:00:00
abstract：:Various lignified materials, including pine cone extract and a commercial lignin, stimulated the morphological change (spreading) of mouse peritoneal macrophages, and their functional maturation as judged by increase in NBT-reducing activity. When these materials were treated with NaClO2 to decompose the phenolic stru...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：BACKGROUND:Prediction of biological behavior is crucial for selection of new therapeutic modalities in GIST. Here, we aimed to assess whether KIT and PDGFRA mutations have survival impact in gastrointestinal stromal tumors (GIST). PATIENTS AND METHODS:Fifty-five Brazilian patients with completely resected GIST were ex...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：BACKGROUND:Several studies have indicated that mimosine, a plant amino acid, is a potential inhibitor of the cell cycle giving rise to growth arrest in G1-phase. These results were mainly derived from in vitro investigations. To date only one paper is available about its applicability in xenografted lung tumor. This st...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:Despite progress in chemotherapeutic agents, non-small cell lung cancers (NSCLC) still have a poor survival rate. Thus, development of new therapeutic strategies, specifically against cancer cells is still required. For this purpose, we treated the non-small cell lung cancer cell line NSCLC-N6 with the natural product...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：:Using the PM3 method, the interactions between chlorpromazine (CPZ) with 2'-deoxyguanosine-5'-monophosphate (dGMP) were examined. We obtained the optimized geometrical structure of each CPZ, dGMP and a CPZ-dGMP system in both aqueous-phase and gaseous-phase and investigated their geometric and electric changes and For...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：BACKGROUND:The cytosine deaminase/5-fluorocytosine (CD/5-FC), strategy for cancer gene therapy shows considerable promise in experimental models but, because CD is a cytosolic enzyme, intracellular production of 5-fluorouracil (5-FU) causes the demise of the transduced cells before cytotoxic concentrations of' 5-FU can...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：AIM:We report on a rare case of appendiceal primary neuroendocrine carcinoma (NEC) and discuss three cases previously described. CASE REPORT:A 58-year-old woman presented with acute abdominal pain and a low-grade fever. She was diagnosed with acute appendicitis and underwent laparoscopic appendectomy. Pathological exa...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2013-06-01 00:00:00
abstract：:F16 is a plant-derived pharmacologically active fraction extracted from Eurycoma longifolia Jack. Previously, we have reported that F16 inhibited the proliferation of MCF-7 human breast cancer cells by inducing apoptotic cell death while having some degree of cytoselectivity on a normal human breast cell line, MCF-10A...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：BACKGROUND/AIM:The aim of this study is to find synergistic effect using FH535 and sorafenib by targeting the RAS/RAF/MAPK and WNT/β-catenin pathways. MATERIALS AND METHODS:3H-Thymidine incorporation assays were performed to address Huh7 and liver cancer stem cell (LCSC) inhibition using FH535 and sorafenib, alone and...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
abstract：:Adenovirus(ADV) mediated thymidine kinase(TK) gene therapy followed by ganciclovir(GCV) administration is widely used in different types of cancer. ACV shares the same mechanism of selective cell killing in ADV/TK positive cells as GCV and can be used at 4.5 times higher doses in patients without significant side effe...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：BACKGROUND:Small cell neuroendocrine cervical carcinoma (SCNCC) is a rare tumor that comprises 1-3% of cervical tumors. SCNCC exhibits clinical and biological characteristics of both cervical neoplasm (such as local aggressiveness and involvement of papillomavirus) and neuroendocrine small cell cancer of any site (such...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-02-01 00:00:00
abstract：BACKGROUND:Intraosseous lipoma remains a poorly-characterized tumor. In paticular, the natural course of intracalcaneus lipoma after conservative treatment and the appropriate surgical modalities are still quite controversial. PATIENTS AND METHODS:We reviewed six symptomatic patients who underwent curettage of intraca...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-02-01 00:00:00
abstract：:An unselected series of 19 colorectal adenocarcinomas obtained from patients treated by surgery at Memorial Sloan-Kettering Cancer Center (MSKCC), New York, USA was investigated for mutations in exons 5, 6, 7 and 8 of the p53 gene. Ten of the tumors revealed at least one somatic mutation either in the exon 5 or 8. Two...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：BACKGROUND/AIM:Advanced anaplastic thyroid cancer (ATC) is a rare, but highly aggressive malignancy, and its prognostic factors need to be further explored. We examined socioeconomic factors' predictive effect for survival performance in stage IV ATC patients. MATERIALS AND METHODS:Using the Surveillance, Epidemiology...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13198
更新日期：2019-02-01 00:00:00
abstract：BACKGROUND:Intradermal or oral administration of lipopolysaccharide derived from Pantoea agglomerans (IP-PA1) has shown prophylactic and antitumor effects without serious side-effects. While it is known that tumor necrosis factor (TNF)-alpha produced by activated macrophages plays an important role in the expression me...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-11-01 00:00:00
abstract：AIM:To analyze how patients experience Stereotactic guided vacuum-assisted breast biopsy (VABB) both physically and mentally. PATIENTS AND METHODS:Two hundred and eleven consecutive women underwent VABB using one of two different biopsy devices (ATEC® and Mammotome®). Patients were queried using a questionnaire. RESU...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：BACKGROUND:The rat model of breast cancer induced by the administration of N-methyl-nitrosourea (NMU) constitutes a useful tool for dissecting the initiation, promotion and progression process of carcinogenesis. Angiogenesis, the recruitment of new blood vessels, is an essential component of the metastatic pathway. Tum...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:The chemotherapeutic agent retinoic acid (RA) inhibits the proliferation and invasion of many tumor types. RA chemotherapy in head and neck squamous cell carcinoma (SCC) patients reduces recurrence and induces regression of premalignant lesions. The effects of RA are mediated by both cytoplasmic and nuclear proteins. ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND:Recent developments in detection of micrometastasis have revealed a considerable incidence of systemic disease in patients who would previously have been diagnosed as having solid tumours only. The purpose of this study was to investigate the prevalence and clinical significance of circulating carcinoma cell...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND:It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. MATERIALS AND METHODS:Hep G...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:To determine the changes in biological features of the prostate during the course of local recurrence of prostate cancer after endocrine therapy, histologic grade, proliferating activity and apoptotic indices were examined in prostate specimens obtained before treatment and at recurrence. A total of 16 patients, who h...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:Since the anti-HER2 monoclonal antibody trastuzumab made its triumphant advance into breast cancer therapy, new selective agents, including pan-HER inhibitors are entering clinical practice. MATERIALS AND METHODS:This study investigates the expression of the four HER-family members, HER1-4, in 48 primary br...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-11-01 00:00:00
abstract：BACKGROUND/AIM:Insulin-like growth factor II mRNA-binding protein 3 (IMP3) plays an important role in the adhesion, invasion, and metastasis of tumor cells. Although emerging evidence suggests that IMP3 promotes tumor progression in several malignancies, the expression of IMP3 and its prognostic implication in adenocar...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13683
更新日期：2019-09-01 00:00:00
abstract：:High-performance liquid chromatography revealed that sodium 5,6-benzylidene-L-ascorbate (SBA), dissolved in distilled water, was gradually decomposed into ascorbic acid and benzaldehyde. Among these three compounds, ascorbic acid showed the most potent cytotoxic activity. The cytotoxic activity of each compound was si...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：BACKGROUND:Despite the key role of mutational analysis in targeted therapy, the difficulty in acquisition of adequate tumor tissues for molecular genotyping in advanced non-small cell lung cancer (NSCLC) has led to the need for a fast and efficient method for detecting genetic alterations for targeted therapy. PATIENT...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
