Abstract：
:The chemotherapeutic agent retinoic acid (RA) inhibits the proliferation and invasion of many tumor types. RA chemotherapy in head and neck squamous cell carcinoma (SCC) patients reduces recurrence and induces regression of premalignant lesions. The effects of RA are mediated by both cytoplasmic and nuclear proteins. In the nucleus, a family of ligand-dependent transcription factors, the retinoic acid receptors (RAR) and the retinoid X receptors (RXR), regulate target gene response to RA. In the cytoplasm, the cellular retinoic acid binding proteins I and II (CRABP) regulate intracellular RA concentration, transport, and metabolism. Alterations in CRABP expression have been shown to affect target gene response and the phenotype of cancer cells. To elucidate the role of these proteins in mediating the RA response, we examined target gene expression and malignant phenotype in SCC25 cells expressing an antisense CRABP II construct. RA induced CRABP II mRNA levels 2 fold in SCC25 cells by transcriptional upregulation. Expression of the antisense construct reduced CRABP II expression to undetectable levels. Inhibition of CRABP II expression resulted in significant downregulation of RA responsive genes. These reductions were the result of decreased transcription from RA responsive promoters. Surprisingly, clones expressing the antisense CRABP construct were less sensitive to RA mediated inhibition of proliferation. These clones were also less invasive in an in vitro invasion assay, likely due to downregulation of matrix metalloproteinase activity. We conclude that CRABP II affects the transcription of RA responsive genes which regulate proliferation and invasion of head and neck SCCs.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Vo HP,Crowe DLsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
217-24issue
1Aeissn
0250-7005issn
1791-7530journal_volume
18pub_type
杂志文章abstract：BACKGROUND:Human DNA topoisomerase I (topo 1) is an essential nuclear enzyme involved in vital cellular processes and the sole target of antitumor drugs of the camptothecin (CPT) family. The CPT derivative topotecan (Tpt, Hycamtin) is currently used in clinic, its effectiveness varying considerably for different types ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：BACKGROUND:Active hexose-correlated compound (AHCC) is an extract of a basidiomycete mushroom that enhances the therapeutic effects and reduces the side-effects of chemotherapy. Our previous studies demonstrated that heat-shock protein 27 (HSP27) was involved in gemcitabine-resistance of pancreatic cancer cells and it ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-11-01 00:00:00
abstract：BACKGROUND:Melanoma cells express the nuclear vitamin D receptor (VDR), indicating that malignant melanoma represents a promising target for treatment with 1,25-dihydroxyvitamin D3 (1,25(OH)(2)D(3)) or its analogs. We previously showed that some melanoma cell lines are resistant to the antiproliferative effects of 1,25...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Tissue-specific expression of 25-hydroxyvitamin D-1alpha-hydroxylase (1alpha-OHase) and vitamin D-hydroxylase (24-OHase) may act as the pivotal link between 25-hydroxyvitamin D3 (25(OH)D3) serum levels and the anticancer effects of 1,25-dihydoxyvitamin D3 (1,25(OH)2D3) and alternative splicing of the enzymes may regul...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-09-01 00:00:00
abstract：:The cyclin-dependent kinase inhibitor (CDK1) p27(kip1) is a negative regulator of cell cycling and has antitumor effects. In our previous study, the recombinant adenovirus expressing wild-type p27(kip1) (Adp27-wt) induced cell cycle arrest and apoptosis, and proved that p27 is a tumor suppressor gene like p53. Another...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-06-01 00:00:00
abstract：:Gn-RH agonists or surgical castration are considered standard treatment for patients affected by metastatic prostate cancer. Despite greater cost, chemical castration is often considered the treatment of choice as it is psychologically better tolerated. We report our experience of one patient undergoing treatment with...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:Peroxisome proliferator activated receptor gamma (PPARgamma) involvement in thyroid tumorigenesis has recently been studied, especially in follicular neoplasms. Conflicting results concerning the regulation of this receptor in human papillary carcinoma have been reported. Therefore, we quantitatively assesse...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：BACKGROUND/AIM:Kallikrein-related peptidases (KLKs) comprise a serine protease family with prominent roles in tissue physiology and disease pathogenesis, including cancer. Previously, we have characterized canine Klk4-10 and -14. Herein, we continue our efforts by characterizing three novel members of the canine family...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
abstract：BACKGROUND/AIM:Many studies have shown an antiproliferative, anti-inflammatory, anti-angiogenetic, and apoptosis-inducing effect of Vitamin D. A vitamin D deficiency has been associated with an increased risk for different types of cancer. This study examined vitamin D 25(OH)D levels in gynaecological cancers in compar...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13983
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:DNA topoisomerase II alpha (topo II alpha) is associated with active cell proliferation of mammalian cells. Topo II alpha overexpression has been reported in a number of human malignancies and is considered to be related to their biological behaviors. MATERIALS AND METHODS:We examined the expression of topo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:We have previously found that in one of two human melanoma cell lines, potent increase in the polyamine catabolizing enzyme, spermidine/spermine N1-acetyltransferase (SSAT), correlate with growth sensitivity to the spermine analog, N1, N12-bis(ethyl) spermine (BESPM). Herein, we examine the generality of this SSAT res...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：BACKGROUND:The aim of this study was to assess the relationship between p53 status and the clinical outcome of patients with advanced ovarian cancer treated with a paclitaxel-based regimen. PATIENTS AND METHODS:The investigation was conducted on 38 patients with FIGO stage III-IV ovarian cancer from whom tumor tissue ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND:Neolymphangiogenesis, a process of lymphatic vessel development in neoplastic tissue, may be a key event in the transmission of cancer cells into lymph nodes. The current study examined the relationship between lymphatic vessel density (LVD) measured by podoplanin (D2-40) expression, clinicopathological para...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-06-01 00:00:00
abstract：BACKGROUND:Hyaluronan is one of the main components of the extracellular matrix. It is synthesized at the cell plasma membrane by specific hyaluronan synthases (HAS). Although a large number of studies have described hyaluronan in pleural effusion from malignant mesothelioma, the source of hyaluronan in malignant mesot...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:In this study, we investigated the cytotoxicities of flavone (F01), 3-hydroxyflavone (F02), 6- hydroxyflavone (F03), 7-hydroxyflavone (F04), 3,6-dihydroxyflavone (F05), 5,7-dihydroxyflavone (F06) and 5,6,7-trihydroxyflavone (F07) to human cancer cells including P- glycoprotein (Pgp)-expressing HCT15 cells and its mult...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：BACKGROUND AND AIM:The Seventh American Joint Committee on Cancer (AJCC)/TNM classification defined that the classification for adenocarcinoma of the esophagogastric junction (AEG) was included in the esophageal category. However, there still remain various classifications in AEG such as the Seventh AJCC/TNM of the eso...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：:The aim of this project was to study the kinetics of both Tl and Tc-99m MIBI in PBM by evaluating tumor to normal tissue ratio in early (E) images acquired within 1/2 hour and delayed (D) images acquired three hours following the i.v. injection of 3 mCi (111 MBq) of Tl and 20 mCi (740 MBq) of MIBI on 2 separate days i...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：AIM:To assess the outcome of patients with vulvar carcinoma unfit for surgery treated with radiotherapy for primary disease and for those with recurrent disease after primary surgery. PATIENTS AND METHODS:The study was conducted on 16 patients with primary disease and 31 with recurrent disease. RESULTS:An objective r...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：:The incidence of breast cancer is increasing rapidly in Taiwan but is still low compared with western countries. The impact of body size factors in western countries inspired us to have a case-control study. A matched case-control study was done on 122 pairs of incident cases of female breast cancer and community cont...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:The pharmacokinetics of platinum in cancer patients were examined following an intravenous infusion of cis-diammine(glycolato) platinum, 254-S. The plasma concentrations of total platinum, which decreased biexponentially after the infusion, were proportional to the dose over the range of 10 to 120 mg/m2, suggesting li...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:To determine the changes in biological features of the prostate during the course of local recurrence of prostate cancer after endocrine therapy, histologic grade, proliferating activity and apoptotic indices were examined in prostate specimens obtained before treatment and at recurrence. A total of 16 patients, who h...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:Axillary dissection is routinely conducted for all patients with sentinel node (SN)-positive breast cancer. Metastasis to non SNs is not often found after axillary dissection in patients with SN-positive breast cancer. Thus, we investigated clinicopathological features, including immune cells in peripheral b...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14445
更新日期：2020-08-01 00:00:00
abstract：:The mouse/human chimeric antibody Ch F11-39, recently generated by ourselves, shows the same high specificity and affinity for carcinoembryonic antigen (CEA) as those of its parental mouse monoclonal antibody. Ch F11-39 is capable of mediating antibody-dependent cell-mediated cytotoxicity (ADCC) with human peripheral ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:We have recently identified ICBP90 as being a protein able to bind in vitro a CCAAT box of the topoisomerase II alpha gene promoter. The aim of the present work was to check whether ICBP90 is able to regulate in vivo topoisomerase II alpha expression in human lung fibroblasts under various proliferating conditions. Tr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：BACKGROUND:In order to decrease surgery-related morbidity, we evaluated the reliability of the evaluation of lymph node metastasis in patients with uterine corpus cancer by positron-emission tomography (PET) with 2-[(18)F]fluoro-2-deoxy-D-glucose (FDG) before surgical staging. MATERIALS AND METHODS:Patients with newly...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：:Gynaecologic oncology day surgery deals primarily with the diagnosis of endometrial, cervical, vulvar and vaginal tumors. Conscious sedation is an important technique used in gynaecologic oncology day surgery. It is often associated with regional anaesthesia. The goals of conscious sedation are to provide effective pa...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2004-11-01 00:00:00
abstract：BACKGROUND:This study aimed to elucidate the prognostic value of vimentin expression in patients with intrahepatic cholangiocarcinoma (ICC). PATIENTS AND METHODS:A retrospective analysis of 21 patients who underwent resection for ICC was conducted. Vimentin expression was positive when single tumour cells or cell clus...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：BACKGROUND:Renal oncocytomas (ROs), and clear cell (RCC) and urothelial carcinomas (UC), are common renal neoplasms. Nicotinamide phosphoribosyltransferase (Nampt) catalyzes the rate-limiting step of NAD+ synthesis and its expression is increased in several tumors. Nampt concomitantly regulates hydrogen sulfide (H2S)-s...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11709
更新日期：2017-07-01 00:00:00
abstract：BACKGROUND/AIM:Occult cancers' reported rates vary from 2-12% and serous tubal intraepithelial carcinomas (STICs) have been identified in 3-12% of the prophylactically removed tubes of women carrying a BRCA mutation. The aim of this study was to evaluate the incidence of tubal minor epithelial atypia (STIL), STIC, and ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11948
更新日期：2017-09-01 00:00:00
abstract：:The recombinant plasmid pBHIV-1 carrying the long terminal repeat (LTR) of the human immunodeficiency virus 1 (HIV-1), linked to the reported chloramphenicol acetyl transferase (CAT) gene, was introduced into human and rat fibroblasts. Stable transfectants were obtained which were resistant to genetecin and expressed ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
