Abstract:
:In this study, we investigated the cytotoxicities of flavone (F01), 3-hydroxyflavone (F02), 6- hydroxyflavone (F03), 7-hydroxyflavone (F04), 3,6-dihydroxyflavone (F05), 5,7-dihydroxyflavone (F06) and 5,6,7-trihydroxyflavone (F07) to human cancer cells including P- glycoprotein (Pgp)-expressing HCT15 cells and its multidrug resistant subline, HCT15/CL02 cells. We also examined the effects of those flavonoids on the cell cycle of these cancer cells. HCT15/CL02 cells did not reveal resistance to all the flavonoids tested in comparison with HCT15 cells. In cell cycle analysis, all the flavonoids tested, except F01 and F04, reduced the G0/G1 population of SF295 cells at growth inhibitory concentrations, and increased G2/M (F02, F03 and F06) or S (F05 and F07) populations. In addition, F02 and F03 decreased the G2/M and G0/G1 population, and increased the S and G2/M population in HCT15 cells, respectively. Meanwhile, in HCT15/CL02 cells, F02 and F03 decreased the G0/G1 populations and increased the S population. In conclusion, we deemed that the flavonoids tested had diverse cytotoxic mechanisms, and exerted their cell growth inhibitory or killing activity by distinctive ways in different cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Choi SU,Ryu SY,Yoon SK,Jung NP,Park SH,Kim KH,Choi EJ,Lee COsubject
Has Abstractpub_date
1999-11-01 00:00:00pages
5229-33issue
6Beissn
0250-7005issn
1791-7530journal_volume
19pub_type
杂志文章abstract::An in vitro cell line (HT 58) has been established from a human (B) NHL xenograft. The lymphoma cells in culture retained their lymphoblastic appearance, DNA-content, IgM/lambda monoclonality and many immunophenotypic markers. The clonal chromosomal abnormalities involved the chromosomes 1, 2, 3 and 14. The cells expr...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::The antineoplastic drug 4'-iodo-4'-deoxydoxorubicin (IDX), a new halogenated anthracycline (1), was examined as a differentiation inducing agent on the human neuroblastoma cell lines TS12 and SK-N-MC. IDX induced morphological and biochemical differentiation and growth inhibition. The effect of a combined treatment of...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract:BACKGROUND:CYP3A4 is the most abundant xenobiotic-metabolizing cytochrome P450 isoform. We examined the structural features of the CYP3A4 molecule with regard to ligand access. MATERIALS AND METHODS:The deleted amino acid sequences of X-ray data sets of CYP3A4s were complemented by molecular modeling techniques. Molec...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-03-01 00:00:00
abstract:BACKGROUND:The description of a new method for the measurement of complexed prostate-specific antigen (cPSA) offers a new approach to the diagnosis of prostate cancer. PATIENTS AND METHODS:We measured PSA (Hybritech and Bayer), free PSA (Hybritech) and complexed PSA (Bayer) in 72 patients with prostate cancer and 128 ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-11-01 00:00:00
abstract:BACKGROUND/AIM:Interleukin-32 (IL32) has been newly-identified as a proinflammatory cytokine. In the present study, we aimed to clarify the clinical role of IL32-positive cells in esophageal squamous cell cancer (ESCC) and regulatory T-cell (Treg) infiltration in the stroma. A total of 179 patients with ESCC who underw...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:Despite the key role of mutational analysis in targeted therapy, the difficulty in acquisition of adequate tumor tissues for molecular genotyping in advanced non-small cell lung cancer (NSCLC) has led to the need for a fast and efficient method for detecting genetic alterations for targeted therapy. PATIENT...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract::The content, composition and physicochemical characteristics of glycosaminoglycans in human rectum and rectum carcinoma were investigated by chemical analyses, enzymic treatments, chromatographic and electrophoretic techniques. The overall glycosaminoglycan content was increased about 2 fold in neoplastic tissues comp...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:Pathological examination of sentinel lymph nodes (SLNs) and non-SLNs in colon cancer is frequently not performed to the same extent. We examined whether non-SLNs were truly negative in tumors with tumor-negative SLNs using reverse transcriptase-polymerase chain reaction (RT-PCR). PATIENTS AND METHODS:RT-PCR...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:18F-FDG positron emission tomography (PET) may alter therapeutic management of patients with cervical cancer because 18F-FDG-positive pelvic and para-aortic lymph nodes are associated with lower progression-free survival and vice versa. However, the accuracy of 18F-FDG PET might be impaired by a preceding ly...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract:AIM:The aim of the present study was to investigate the impact of cyclin E expression on cancer-specific survival, as well as on conventional clinocopathological and prognostic factors in endometrial endometrioid adenocarcinoma. MATERIALS AND METHODS:The study consisted of 211 patients surgically treated for endometri...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-06-01 00:00:00
abstract:AIM:To elucidate the behavior of 1 phosphatidylinositol 4,5 bisphosphate (PIP2) 5 phosphatase (PIP2 5-Pase, EC 3.1.3.36) and 1 phosphatidylinositol 4 phosphate (PIP) 4-phosphatase (PIP 4-Pase) in cancer cells, the steady state activities of PIP2 5-Pase and PIP 4-Pase were determined in the particulate fractions of rat ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract:BACKGROUND:NSC697923, a ubiquitin-conjugating enzyme E2 (UBE2) inhibitor, was suggested as an agent to degrade hypoxia-inducible factor 1 alpha subunit (HIF1α), a key factor in radiation resistance. We attempted to clarify whether NSC697923 could overcome radiation resistance. MATERIALS AND METHODS:Radiation resistanc...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11582
更新日期:2017-05-01 00:00:00
abstract::The metabolic transformations of labeled pyridoxine by hepatoma cells were studied. Buffalo rats were fed ad libitum a commercially prepared pyridoxine sufficient diet for 20 days at which time they were inoculated intramuscularly with hepatoma 7777 cells in both hind leg muscles. After tumor development, 50 microCi [...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::P-glycoprotein has been identified in mast cells stabilized in culture as well as in rat peritoneal mast cells, and is primarily concentrated on the granular membrane. This study aimed to define the role of this protein in the transport and accumulation of doxorubicin in mast cell granules and in its histamine releasi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::Wet ashing of K562 cell suspensions by means of a sulphonitroperchloric acid digestion for electrothermal atomic absorption spectrometry of platinum has been achieved. The limit of detection was about 1 ng platinum per 10(6) cells. Platinum concentrations in K562 cells were measured after exposure to platinum coordina...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:In order to develop a multi-marker RT-PCR, which as such may be more sensitive than a single marker assay for the detection of disseminated tumor cells, we evaluated six RT-PCR markers: cytokeratin 20 (CK20), carcinoembryonic antigen (CEA), guanylyl cyclase C (GCC), epidermal growth factor receptor (EGFR), m...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::The expression of p53 and mdm-2 proteins was analysed in parrafin sections from 39 cases of Hodgkin's disease (HD) and compared to the presence of Epstein-Barr Virus (EBV). P53 protein was found in Hodgkin and Reed-Sternberg (HRS) cells in 12/39 cases. Mdm-2 protein was found in HRS cells in 10/39 cases. EBV-encoded E...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::Adriamycin (ADM) is an oncostatic of the anthracycline family with confirmed experimental and clinical efficiency. This antitumoral drug has been reported to stimulate macrophage activity and is able to induce apoptosis (AP) in some tumour cells. The objective of the present work was to investigate if in vivo administ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract:BACKGROUND/AIM:Recent progress with BRAF and immune checkpoint inhibitors has dramatically changed the treatment landscape of metastatic melanoma (MM). The limited duration of responses to new agents, however, justifies the, still important, role of chemotherapy in the management of MM. This study prospectively explore...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-02-01 00:00:00
abstract::The poor prognosis of advanced gastric carcinoma requires new therapeutic approaches. Among these, the specific activation of resting lymphocytes by bispecific antibodies may be promising. Here we describe the generation and function of a bispecific monoclonal antibody (bi-mab) with specificity for CD3 and for the tum...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract::Using gas chromatography, the fatty acid profile has been measured in subcutaneous fat obtained from women undergoing biopsy for benign and malignant breast conditions. There was a similar proportion of saturated and unsaturated fatty acids in both types or fat, suggesting that long-term differences in fatty acid cons...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1987-11-01 00:00:00
abstract:BACKGROUND/AIM:Phenolic compounds isolated from Dendrobium ellipsophyllum Tang & Wang (Orchidaceae) have been shown to possess potential pharmacological activity; however, their anticancer as well as anti-metastasis activities are largely unknown. The aim of the present study was to isolate active compounds from D. ell...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-11-01 00:00:00
abstract::Our previous work showed that low-power wide-band millimeter waves (MMW) inhibit the growth of the MCF-7 human breast carcinoma cell line, also causing a marked reduction of the density of microvilli at the apical membrane of the MCF-7 cells, as revealed by scanning electron microscopy. The aim of the present work was...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
abstract::A new cell line, CC3/CUHK3, was established from a squamous carcinoma of the uterine cervix from a Chinese patient. Light microscopy of the cell line revealed keratin and electron microscopy demonstrated tonofilaments and desmosomes, thereby confirming its epithelial nature. The population doubling time of CC3/CUHK3, ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Non-small-cell lung cancer (NSCLC) ranks as a leading cause of cancer-related death globally. Brain metastases are a frequent complication of NSCLC, with 25-40% of patients developing brain metastases during the course of the disease, often within the first 2 years after diagnosis of the primary tumor. Improvements in...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND:The objective of the present study was to investigate how oxidative status influences the effectiveness of cytotoxicity of artemisinin towards cancer cells. It is hypothesized that antioxidants would reduce, whereas pro-oxidants would enhance, cytotoxicity. MATERIALS AND METHODS:Molt-4 human leukemia cells ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-04-01 00:00:00
abstract::Radiation therapy plays a critical role in women with advanced-stage cervical cancer worldwide, particularly in developing countries, and most of the time it may be the only available treatment. The efficacy of radiation largely depends on the radiosensitivity of the tumor. The high radiation dose associated with ther...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2014-09-01 00:00:00
abstract::Information on chromosomal structural rearrangements in leukemia and solid tumors was retracted from a computerized databank and the literature. A binomial test procedure was used to determine the statistical significance of the observed numbers of breaks. Some 131 human cancer-specific chromosomal structural rearrang...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::The structural specificities of the natural polyamines putrescine (Put), spermidine (Spd) and spermine (Spm) for cell growth are rather stringent, suggesting that appropriate structural analogues of these polycations could serve as potential antineoplastic agents via polyamine antagonism. Norspermidine (Nspd), a homol...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1988-07-01 00:00:00
abstract::We have developed a sensitive enzyme-linked immunosorbent assay (ELISA) procedure that uses a commercially-available anti-BrdUrd antibody for the quantitation of BrdUrd substituted into DNA. In our assay, 50% displacement occurs at 0.89 nM of BrdUrd in 2.2% BrdUrd-substituted DNA, which is equivalent to 2.47 ng of Brd...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00