Abstract：
BACKGROUND:Hyaluronan is one of the main components of the extracellular matrix. It is synthesized at the cell plasma membrane by specific hyaluronan synthases (HAS). Although a large number of studies have described hyaluronan in pleural effusion from malignant mesothelioma, the source of hyaluronan in malignant mesothelioma has been subject to controversy. MATERIALS AND METHODS:The mRNA expression of all three HAS in malignant mesothelioma cells was studied using RT-PCR. The hyaluronan production in culture medium of malignant mesothelioma cells was also examined using high-performance liquid chromatography (HPLC). RESULTS:We found that 9/10 malignant mesothelioma cell lines and one primary culture of malignant mesothelioma cells expressed HAS-1, while 10/10 malignant mesothelioma cell lines and one primary culture of malignant mesothelioma cells expressed HAS-2 and HAS-3. In addition, we demonstrated hyaluronan in the culture medium of 6 out of 10 malignant mesothelioma cell lines and one primary culture of malignant mesothelioma cells. CONCLUSION:Our results show that malignant mesothelioma cells express all three HAS and synthesize hyaluronan. The expression of HAS isoforms and hyaluronan in malignant mesothelioma cells in cultures and previous observations by other investigators indicate that these cells are, at least in part, responsible for hyaluronan synthesis in vivo.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Liu Z,Dobra K,Hauzenberger D,Klominek Jsubject
Has Abstractpub_date
2004-03-01 00:00:00pages
599-603issue
2Beissn
0250-7005issn
1791-7530journal_volume
24pub_type
杂志文章abstract：BACKGROUND/AIM:Several epidemiological studies have reported the chemopreventive potential of biochanin A, in cancer development and progression. We investigated the anticancer potential of combination of biochanin A and temozolomide against U-87 MG and T98 G [glioblastoma multiforme (GBM)] cells. MATERIALS AND METHOD...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13079
更新日期：2019-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12723
更新日期：2018-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-10-01 00:00:00
abstract：BACKGROUND:Increasing evidence suggests that COX-2 promotes carcinogenesis but it is controversial as to whether COX-2 expression is a prognostic factor for rectal carcinoma. MATERIALS AND METHODS:Data of 62 consecutive patients with rectal carcinoma in UICC-stages I-III, which were curatively resected between 1995 an...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
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journal_title：Anticancer research
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doi：
更新日期：2000-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：2002-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,多中心研究
doi：
更新日期：2016-06-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,随机对照试验
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更新日期：2020-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
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更新日期：2018-02-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1987-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-06-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
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pub_type： 临床试验,杂志文章
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更新日期：2007-03-01 00:00:00
