Abstract：
BACKGROUND:The cytosine deaminase/5-fluorocytosine (CD/5-FC), strategy for cancer gene therapy shows considerable promise in experimental models but, because CD is a cytosolic enzyme, intracellular production of 5-fluorouracil (5-FU) causes the demise of the transduced cells before cytotoxic concentrations of' 5-FU can be achieved within the extracellular milieu. MATERIALS AND METHODS:A soluble secreted form of CD was constructed and evaluated compared to intracellular CD in vitro and in vivo. RESULTS:The secreted form of CD temporarily spared transduced cells and enhanced accumulation of extracellular 5-FU. Cytosolic CD produced rapid inhibition of thymidylate synthase and cell death before significant extracellular concentrations of 5-FU developed. Finally, tumors expressing the secreted form of CD had an improved response to 5-FC treatment compared to tumors expressing intracellular CD. CONCLUSION:Further evaluation of extracellular expression of CD for enzyme/prodrug therapy may provide improvements in this commonly studied gene therapy strategy.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Rehemtulla A,Hamstra DA,Kievit E,Davis MA,Ng EY,Dornfeld K,Lawrence TSsubject
Has Abstractpub_date
2004-05-01 00:00:00pages
1393-9issue
3aeissn
0250-7005issn
1791-7530journal_volume
24pub_type
杂志文章abstract：:The effect of some new antiestrogens (ICI 164384, ICI 182780, Ly 133314 and Ly 117018) on the growth of a panel of breast cancer cell lines (MDA-MB 231, BT20, MCF7 and T47D) was studied. Their antiestrogenic activity was investigated in the presence of a physiologic concentration of estradiol and compared with the eff...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:The purpose of this study was to determine whether the degree of anchorage-independent growth of rodent or human cells in increasing concentrations of agarose correlated with successful transfection of the cells with an activated c-Ha-ras oncogene and tumorigenicity in nude mice. NIH 3T3 cells, C3H 10T1/2 fibroblasts,...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND/AIM:Stage-specific embryonic antigen-4 (SSEA-4) expression is associated with malignant aggressiveness and is useful as a marker for identifying cancer stem cells. Our aim was to assess the relationship between hormonal therapy and SSEA-4 expression in prostate cancer (PC). MATERIALS AND METHODS:SSEA-4 expr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14569
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND:Since specific epidermal growth factor receptor (EGFR) somatic mutations have been demonstrated to influence the response to anti-EGFR therapy in non-small cell lung cancer, EGFR gene mutational analysis of different types of neoplasm is under investigation. PATIENTS AND METHODS:EGFR protein expression and ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：BACKGROUND/AIM:Detection of circulating tumor cells in the perioperative period predicts poor prognosis in patients with colorectal cancer. There is only one Food and Drug Administration-approved method for such detection, CellSearch, for which results have been inconsistent. A new immunological method, CytoTrack, has ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11666
更新日期：2017-06-01 00:00:00
abstract：:Intravenous administration of sodium benzylideneascorbate (SBA) rapidly necrotized inoperable human lung cancer, and induced degeneration of 3'-methyl-4-dimethylaminoazobenzene-induced rat hepatocellular carcinoma (vacuolar, eosinophilic degeneration, nuclear debris) without affecting the serum glutamic oxaloacetic tr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-07-01 00:00:00
abstract：:The antitumor activity of (2''R)-4'-O-tetrahydropyranyl adriamycin (pirarubicin; THP) was assessed using human gastric cancer cell lines in vitro and in vivo. The cytotoxicity of THP on MKN-28 and MKN-45 was superior to that of adriamycin (ADM) as detected by a growth assay with an MTT colorimetric endpoint. When the ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：BACKGROUND:The tyrosine kinase inhibitors (TKI) sunitinib and imatinib were shown to induce macrocytosis in patients with renal cell cancer (RCC) and gastrointestinal stromal tumors (GIST), presumably through inhibition of the c-KIT dependent signaling pathway of erythroid progenitor cells of the bone marrow. PATIENTS...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
abstract：:Thirty advanced breast cancer patients received Vinorelbine (VNB) according to two different schedules: a weekly i.v. bolus of 30 mg/m (14 patients) or a continuous infusion (CI) schedule comprising 8 mg i.v. bolus on day 1 followed by 8 mg/m2/die CI day 1 to 4, q 21 days (16 patients). 36% of patients had received at...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:A new cell line, CC3/CUHK3, was established from a squamous carcinoma of the uterine cervix from a Chinese patient. Light microscopy of the cell line revealed keratin and electron microscopy demonstrated tonofilaments and desmosomes, thereby confirming its epithelial nature. The population doubling time of CC3/CUHK3, ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND/AIM:The present study aimed to identify hypoxia-regulated microRNAs (HRMs) in vitro and investigate the clinical role of candidate HRMs in patients with gastroesophageal cancer (GEC). MATERIALS AND METHODS:microRNA expression changes induced by hypoxia in human GEC cell lines were measured with microarrays ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-02-01 00:00:00
abstract：BACKGROUND/AIM:Anthracyclines have been proven able to reduce the activity of vinca alkaloids by induction of cell-cycle arrest. The present study aims at identifying the critical initiation steps of signal transduction which transduce the inhibitory effects on the cytotoxicity of vinca alkaloids. MATERIALS AND METHOD...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-12-01 00:00:00
abstract：BACKGROUND:Adverse outcomes in breast cancer patients treated with recombinant human erythropoietin (rhEpo) have been linked to the expression of Epo-receptors (EpoR) in cancer cells, although limited data on the clinical significance of these observations are available. PATIENTS AND METHODS:Tissue samples from 107 pa...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：:The p53 tumor suppressor protein is a pivotal molecule in the protection against carcinogenesis and is inactivated in a plethora of human neoplasms, p53 prevents the accumulation of genomic alterations by inducing cell-cycle arrest or programmed cell death in response to genotoxic signals such as DNA damage and stress...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1999-09-01 00:00:00
abstract：BACKGROUND:Nogo-B was recently shown to be involved in proliferation, apoptosis and invasiveness of cancer cells, whereas its specific receptor (NgBR) was found to be up-regulated in estrogen receptor-α positive breast cancer. No data are currently available concerning their expression in non-small cell lung carcinomas...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND/AIM:AKT, also known as protein kinase B (PKB), is an established therapeutic target in cancer and its inhibitors are increasingly designed. The anti-cancer potential of a compound class naphthoquinones has been constantly realized. The current work aimed to explore AKT1 inhibitors from 1,4-naphthoquionone de...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13532
更新日期：2019-07-01 00:00:00
abstract：BACKGROUND/AIM:Advanced anaplastic thyroid cancer (ATC) is a rare, but highly aggressive malignancy, and its prognostic factors need to be further explored. We examined socioeconomic factors' predictive effect for survival performance in stage IV ATC patients. MATERIALS AND METHODS:Using the Surveillance, Epidemiology...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13198
更新日期：2019-02-01 00:00:00
abstract：BACKGROUND:To investigate the differences between two rat yolk sac tumor cell lines with different radiosensitivities and p53 status in sensitivity to high-LET heavy-ion beam and in sensitizing effect of etoposide or cisplatin in combination with heavy-ion beam. MATERIALS AND METHODS:NMT-1 (wild-type p53 cell) is a pa...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：:Chronic treatment with Sasa Health, extract of bamboo grass leaves obtained by alkaline hydrolysis, in drinking water at the concentration of 0.044%-0.088% Fe-Chlorophyllin Na resulted in the significant inhibition of both development and growth of spontaneous mammary tumours in a high mammary tumour strain of SHN vir...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:E-selectin which is expressed on endothelial cells plays an important role in the adhesion of cancer cells to the vascular endothelium, being the ligand for a carbohydrate antigen expressed on cancer cells. In this study, the clinical usefulness of this protein was examined. E-selectin was expressed on the endothelial...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:Pegylated liposomal doxorubicin is associated with a lower risk of cardiotoxocity than conventional formulations of doxorubicin, allowing the use of higher cumulative doses. In this Phase II study, 25 patients aged over 70 years (median 79, range 75-82 years) with aggressive non-Hodgkin's lymphoma (NHL) (International...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：2002-05-01 00:00:00
abstract：BACKGROUND:The epithelial-mesenchymal transition is a critical early event in the invasion and metastasis of many types of cancer, including colorectal cancer (CRC). Chronic inflammation is an inducer of several cancer types and inflammatory cytokines have been implicated in tumor invasion. MATERIALS AND METHODS:Human...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-11-01 00:00:00
abstract：BACKGROUND:The proportion of geriatric gastric cancer (GC) patients with comorbidities has been increasing in Japan. This study was designed to evaluate the prognostic influence of the extent of lymph node dissection (END) and perioperative morbidities in patients with GC. PATIENTS AND METHODS:Between 2008 and 2013, t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
abstract：:We report a 64-year-old woman with a 9-cm liver tumor in the left lateral section. The patient had neither hepatitis B or C virus infection, nor cirrhosis. Carbohydrate antigen 19-9 (CA 19-9) level was 1,889 U/ml. We also suspected bulky hilar lymph node metastasis, and a left lateral sectionectomy without lymph node ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13327
更新日期：2019-04-01 00:00:00
abstract：:Eighty one patients with breast cancer stages I, II and III (T3a) were given adjuvant treatment with tamoxifen (Nolvadex), chemotherapy, or both. Most patients had estrogen and progesterone receptors measurement. Twenty one (63.6%) patients of the tamoxifen group were node positive, all 25 (100%) of the chemotherapy p...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：BACKGROUND:In 1979, Montgomery and Asberg introduced an inventory for screening of depression. To our knowledge, the associations between the MADRS and the risk of breast cancer (BC) are rarely considered together in a prospective study. PATIENTS AND METHODS:In an extension of the Kuopio Breast Cancer Study, 115 women...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：:AIDS-related Epstein-Barr virus (EBV)-associated T cell lymphomas are emerging as a new, distinct histopathological entity. The pathway whereby EBV infects T cells as well as the initial EBV transcriptional program in T cells has not been established. In order to shed light on the early events of the EBV infection of ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:In the present study, we report that ginsenoside-Rg5 (G-Rg5), a newly discovered diol-containing ginsenoside, blocks the cell cycle of human hepatoma SK-HEP-1 cells via the down-regulation of cyclin E-dependent kinase activity. The results from flow cytometric analyses show that G-Rg5 arrests the cell cycle of SK-HEP-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：BACKGROUND:Pemetrexed and erlotinib represent novel agents for the treatment of non-small cell lung cancer (NSCLC). The role of sequential treatment in NSCLC has not been elucidated yet. We compared the efficacy of second-line pemetrexed followed by third-line erlotinib (P-E) to treatment with the reverse sequence (E-P...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：:Adrenocortical carcinoma (ACC) is a rare neoplasm with a poor prognosis. Prognostic factors are needed to identify patients who should be treated aggressively and those for which a less aggressive approach is warranted. As a result of advances within the field of immunohistochemistry, investigations of Ki-67, PCNA, IG...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
