Engineering challenges in cell-encapsulation technology.

abstract：:The field of regenerative medicine has blossomed in recent decades. However, the ultimate goal of tissue regeneration - replacing damaged or aged cells with healthy functioning cells - still faces a number of challenges. In particular, better understanding of the role of the cellular niche in shaping stem cell phenoty...

journal_title：Trends in biotechnology

authors： Del Sol A,Okawa S,Ravichandran S

更新日期：2019-07-01 00:00:00

abstract：:The lantibiotics are a unique class of bacterium-derived peptide antibiotics, all of which contain the rare amino acid lanthionine, as well as a number of other nonprotein amino acids. Unlike most other peptide antibiotics, lantibiotics are produced on the ribosome as a prepeptide, and a series of post-translational m...

journal_title：Trends in biotechnology

authors： Jack RW,Sahl HG

更新日期：1995-07-01 00:00:00

abstract：:Discussions on research quality and reproducibility are appearing in the pages of scientific journals with heightened significance and gaining media attention. Many institutions have developed guidelines to address the topic of quality in basic research, but questions remain about how best to implement and monitor com...

journal_title：Trends in biotechnology

authors： Riedl DH,Dunn MK

更新日期：2013-10-01 00:00:00

abstract：:Plant-based environmental remediation, or phytoremediation, has been widely pursued in recent years as a favorable clean-up technology and is an area of intensive scientific investigation. For the vast majority of field applications, vegetative 'phyto-crops' are selected specifically for their capacity for site decont...

journal_title：Trends in biotechnology

authors： Rugh CL

更新日期：2004-10-01 00:00:00

abstract：:Advances in high-throughput DNA-sequencing techniques have given us the unprecedented ability to rapidly determine the nucleotide sequences of entire bacterial genomes. The application of these methods to the genomes of microbial pathogens, combined with efficient analytical tools and genome-scale approaches for study...

journal_title：Trends in biotechnology

authors： Smith DR

更新日期：1996-08-01 00:00:00

abstract：:Innovative fermentation processes are necessary for the cost-effective production of bulk chemicals from renewable resources. Current microbial processes are either anaerobic processes, with high yield and productivity, or less-efficient aerobic processes. Oxygen utilization plays an important role in energy generatio...

journal_title：Trends in biotechnology

authors： Weusthuis RA,Lamot I,van der Oost J,Sanders JP

更新日期：2011-04-01 00:00:00

abstract：:Europe has led many aspects of gene research and yet it has been unable to translate these discoveries into a globally dominant industrial sector. There are valid societal, political and financial reasons for its reluctance to deploy agricultural biotechnology but this reluctance might have unintended consequences. It...

journal_title：Trends in biotechnology

authors： Enriquez J

更新日期：2001-04-01 00:00:00

abstract：:Targeting apoptotic cell death pathways provides wide-ranging opportunities for the discovery and development of novel drugs. Some targeted therapies that selectively induce apoptosis in cancer cells are already marketed, and numerous pro-apoptotic drugs for treating cancer are currently being developed. The anti-apop...

journal_title：Trends in biotechnology

authors： Alam JJ

更新日期：2003-11-01 00:00:00

abstract：:The well-studied antigen-combining sites of antibody molecules hold considerable promise as a model system for the design of bioactive peptides. These small, immunoglobulin-derived peptides can be used in the development of alternative treatments for disease and in diagnostic strategies. The general principles derived...

journal_title：Trends in biotechnology

authors： Dougall WC,Peterson NC,Greene MI

更新日期：1994-09-01 00:00:00

abstract：:Recent advances in the extraction and purification of decellularized extracellular matrix (dECM) obtained from healthy or malignant tissues open new avenues for engineering physiomimetic 3D in vitro tumor models, which closely recapitulate key biomolecular hallmarks and the dynamic cancer cell-ECM interactions in the ...

journal_title：Trends in biotechnology

authors： Ferreira LP,Gaspar VM,Mano JF

更新日期：2020-12-01 00:00:00

abstract：:Engineered tissues need a vascular network to supply cells with nutrients and oxygen after implantation. A network that can connect to the vasculature of the patient after implantation can be included during in vitro culture. For optimal integration, this network needs to be highly organized, including venules, capill...

journal_title：Trends in biotechnology

authors： Rouwkema J,Khademhosseini A

更新日期：2016-09-01 00:00:00

abstract：:Molecular systems in which fluorescence switches between 'on' and 'off' states when driven by chemical stimuli can be designed according to a few principles. The photochemical mechanisms examined are photoinduced electron transfer, internal charge transfer and excimer formation, with emphasis on the first category. Th...

journal_title：Trends in biotechnology

authors： de Silva AP,Fox DB,Moody TS,Weir SM

更新日期：2001-01-01 00:00:00

abstract：:Challenges in demonstrating interchangeability and safety, as well as the ongoing evolution of regulations governing biosimilars, have meant that the development of the biosimilars industry has not been, and will not be, a carbon copy of the generics industry. Complexity in the development process reduces the cost adv...

journal_title：Trends in biotechnology

authors： Konara CS,Barnard RT,Hine D,Siegel E,Ferro V

更新日期：2016-01-01 00:00:00

abstract：:Aroma emissions from living cells can provide valuable information about the metabolic and physiological condition of those cells. Electronic noses are chemical gas-sensor arrays that use artificial neural network models to evaluate aromas. They can interpret the complex aroma information emitted from cultures of bact...

journal_title：Trends in biotechnology

authors： Bachinger T,Mandenius CF

更新日期：2000-12-01 00:00:00

abstract：:The BRICS countries (Brazil, Russia, India, China, South Africa) account for 25% of global biotechnology patents. To understand the current and future landscape of the domain, it is important to better understand the capacity of these contributors. Here, we consider the thematic priorities, strategies, and key players...

journal_title：Trends in biotechnology

authors： Streltsova E,Linton JD

更新日期：2018-07-01 00:00:00

abstract：:Selective delivery of lethal levels of drugs to tumors, without concomitant damage to normal tissues, is a major challenge in cancer chemotherapy. Prodrugs used in conjunction with enzyme-monoclonal antibody conjugates that can target tumors and convert prodrugs to their active drug forms in situ, offer exceptional pr...

journal_title：Trends in biotechnology

authors： Huennekens FM

更新日期：1994-06-01 00:00:00

abstract：:Many organisms have the ability to form spores, a remarkable phase in their life cycles. Compared with vegetative cells, spores have several advantages (e.g. resistance to toxic compounds, temperature, desiccation and radiation) making them well suited to various applications. The applications of spores that first spr...

journal_title：Trends in biotechnology

authors： Wolken WA,Tramper J,van der Werf MJ

更新日期：2003-08-01 00:00:00

abstract：:Genome engineering is crucial for answering fundamental questions about, and exploring practical applications of, microorganisms. Various microbial genome-engineering tools, including CRISPR/Cas-enhanced homologous recombination (HR), have been developed, with ever-improving simplicity, efficiency, and applicability. ...

journal_title：Trends in biotechnology

authors： Wang Y,Liu Y,Zheng P,Sun J,Wang M

更新日期：2021-02-01 00:00:00

abstract：:Wheat is one of the major staple food crops grown worldwide; however, productivity in cereal crops has not kept pace with the world population growth. A significant increase in wheat production (>40% by 2020) is needed simply to keep up with the growing demand. This increase is unlikely to be achieved by conventional ...

journal_title：Trends in biotechnology

authors： Bhalla PL

更新日期：2006-07-01 00:00:00

abstract：:Hydrophobic charge induction chromatography using 4-mercapto-ethyl-pyridine as the ligand is an effective method for the separation of antibodies from a variety of feedstocks. Antibodies are adsorbed in physiological conditions without preliminary concentration. Desorption occurs when the pH is lowered, thus inducing ...

journal_title：Trends in biotechnology

authors： Boschetti E

更新日期：2002-08-01 00:00:00

abstract：:The discovery of targets that are sufficiently robust to yield marketable therapeutics is an enormous challenge. Through the years, several approaches have been used with varying degrees of success. These include target-independent screening of tumor-derived cell lines (disease-dependent), reductionist approaches to i...

journal_title：Trends in biotechnology

authors： Ryan TE,Patterson SD

更新日期：2002-12-01 00:00:00

abstract：:Microbial consortia have been used in biotechnology processes, including fermentation, waste treatment, and agriculture, for millennia. Today, synthetic biologists are increasingly engineering microbial consortia for diverse applications, including the bioproduction of medicines, biofuels, and biomaterials from inexpe...

journal_title：Trends in biotechnology

authors： McCarty NS,Ledesma-Amaro R

更新日期：2019-02-01 00:00:00

abstract：:The application of recombinant-DNA methods for the production of therapeutic proteins has, over the past decade, driven the development of new technology for the analysis and characterization of biological molecules. High performance capillary electrophoresis (HPCE) has generated enormous interest among biochemists, a...

journal_title：Trends in biotechnology

authors： Frenz J,Hancock WS

更新日期：1991-07-01 00:00:00

abstract：:One of the goals of synthetic biology is to build regulatory circuits that control cell behavior, for both basic research purposes and biomedical applications. The ability to build transcriptional regulatory devices depends on the availability of programmable, sequence-specific, and effective synthetic transcription f...

journal_title：Trends in biotechnology

authors： Jusiak B,Cleto S,Perez-Piñera P,Lu TK

更新日期：2016-07-01 00:00:00

abstract：:Hydrogels are 3D hydrophilic polymer networks that absorb and hold huge amounts of water. Although hydrogels have traditionally been synthesized using chemical and physical methods, rapid developments in enzyme technology that, like chemical-based methods, enable the formation of stable covalent bonds are fast emergin...

journal_title：Trends in biotechnology

authors： Guebitz GM,Nyanhongo GS

更新日期：2018-10-01 00:00:00

abstract：:The generation of genetically engineered (GE) foods has been raising several concerns and controversies that divide not only the general public but also the scientific community. The fear and importance of the new technology, as well as commercial interests, have supported many of the ongoing discussions. The recent i...

journal_title：Trends in biotechnology

authors： Batista R,Oliveira MM

更新日期：2009-05-01 00:00:00

abstract：:Feedstocks that deserve serious consideration for fuels and chemicals are sugarcane, corn, trees and algae. Commercialization of biomass refining is imminent but the wild claims of those who think that bioenergy can replace much of our dependence on foreign oil are appalling. It is naive to view biomass as the panacea...

journal_title：Trends in biotechnology

authors： Bungay HR

更新日期：2004-02-01 00:00:00

abstract：:Building robust manufacturing processes from biological components is a task that is highly complex and requires sophisticated tools to describe processes, inputs, and measurements and administrate management of knowledge, data, and materials. We argue that for bioengineering to fully access biological potential, it w...

journal_title：Trends in biotechnology

authors： Sadowski MI,Grant C,Fell TS

更新日期：2016-03-01 00:00:00

abstract：:Non-natural, functional RNA molecules, such as short interfering (si) RNAs, aptazymes, maxizymes and intramers, allow modulation of gene function at the mRNA or protein level. This review discusses recent advances made in the expression and application of these functional RNAs and illustrates how engineered, intracell...

journal_title：Trends in biotechnology

authors： Famulok M,Verma S

更新日期：2002-11-01 00:00:00

abstract：:Success in synthetic biology and metabolic engineering is quickly becoming 'test' limited within the design-build-test cycle. Commonly used methods for high-throughput screening, including fluorescence-activated cell sorting (FACS) and microtiter plates, have intracellular product and throughput limitations. A growing...

journal_title：Trends in biotechnology

authors： Bowman EK,Alper HS

更新日期：2020-07-01 00:00:00