Neuroactive steroids modulate GABA inhibition of hypothalamic somatostatin release.

Abstract:

:The reduced steroids 3 alpha-hydroxy-5 alpha-pregnan-20-one (allopregnanolone) and 3 alpha,21-di-hydroxy-5 alpha-pregnan-20-one (allotetrahydroDOC) are potent ligands of GABAA receptors. This study examined the possible modulatory effect of these metabolites on GABA inhibition of somatostatin release in cultured hypothalamic neurones. Allopregnanolone potentiates GABA inhibition, and reversed picrotoxin and bicuculline-induced augmentation of somatostatin release in a dose-dependent manner. AllotetrahydroDOC also inhibits the stimulated release induced by the antagonists, but did not modify that induced by depolarizing concentrations of K+. Pregnenolone sulphate had no effect on picrotoxin-induced somatostatin release. These findings clearly establish that 3 alpha-hydroxysteroids modulate GABA inhibition of hypothalamic somatostatin release.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Tapia-Arancibia L,André M,Belmar J,Arancibia S

doi

10.1097/00001756-199510020-00025

subject

Has Abstract

pub_date

1995-10-02 00:00:00

pages

1927-31

issue

14

eissn

0959-4965

issn

1473-558X

journal_volume

6

pub_type

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