Abstract:
:We previously reported that RU486 can reverse progesterone (P)-induced suppression of the estrogen receptor (ER) in the uterus of pregnant rhesus monkeys, but we did not determine whether estrogen (E) could act through its receptor in the presence of P and RU486. To pursue this question, we treated spayed rhesus monkeys with various hormonal regimens and evaluated the effects of E in the oviduct and endometrium, with and without RU486 treatment, on ER and progestin receptor (PR) levels, morphology, apoptosis, and degree of proliferation. The latter was assessed immunocytochemically with a monoclonal antibody (Ki-67) against a nuclear antigen present only in proliferating cells. Animals were treated for 2 weeks with 17 beta-estradiol (E2) and then for 2 weeks with E2 plus P to produce a regressed oviduct and a secretory endometrium. The animals were then treated for 2 more weeks with four different treatments, as follows: I) E2, P, and vehicle; II) E2 and vehicle; III) E2, P, and RU486; and IV) E2 and RU486 (n = 4 each). In group I, menstruation did not occur, and the endometrium exhibited stromal cell enlargement, extensive development of the spiral arteries, few Ki-67-positive cells, and low levels of ER and PR. Oviducts in group I remained regressed, Ki-67-positive epithelial cells were few, and levels of ER and PR were low. In contrast, in groups II, III, and IV, the oviducts had responded to E2 and were fully ciliated and secretory, with elevated levels of ER and nuclear PR. All animals in these three groups menstruated and then regenerated their endometrium. The regenerated endometria expressed elevations in ER, nuclear PR, and epithelial Ki-67 index. However, the endometria of RU486-treated monkeys in groups III and IV had significantly more epithelial cell death by apoptosis, increased stromal cell compaction, and fewer Ki-67-positive stromal cells than in the E2 controls (group II). In group IV, RU486 caused a significant decrease in endometrial weight. Thus, RU486 blocked P action and allowed E2 to act in a normal fashion in the oviduct and endometrium on several end points, but it also had antiproliferative effects that opposed E2 action, especially in the endometrium.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Slayden OD,Hirst JJ,Brenner RMdoi
10.1210/endo.132.4.8462480subject
Has Abstractpub_date
1993-04-01 00:00:00pages
1845-56issue
4eissn
0013-7227issn
1945-7170journal_volume
132pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The present study was designed to investigate the effect of PTH on calcium (Ca2+) transport through the luminal membrane of proximal and distal rabbit tubule segments. Proximal tubule and distal tubule segment suspensions were incubated with the hormone, and the luminal membranes were subsequently purified. Incubation...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.1.7506210
更新日期:1994-01-01 00:00:00
abstract::The LHX3 transcription factor is required for pituitary and nervous system development in mammals. Mutations in the human gene are associated with hormone-deficiency diseases. The gene generates two mRNAs, hLHX3a and hLHX3b, which encode three proteins with different properties. Here, the cis elements and trans-acting...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0970
更新日期:2006-01-01 00:00:00
abstract::Neonatal thymectomy at 3 days of age in mice results in ovarian dysgenesis, characterized by early follicular and oocyte loss, followed by a high incidence of ovarian tumorigenesis. Thymectomized animals with dysgenetic ovaries had extremely high levels of circulating PRL and gonadotropins before the appearance of the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-6-2375
更新日期:1981-06-01 00:00:00
abstract::Early postnatal hyperalimentation has long-term implications for obesity and developing renal disease. Suppressor of cytokine signaling (SOCS) 3 inhibits phosphorylation of signal transducer and activator of transcription (STAT) 3 and ERK1/2 and thereby plays a pivotal role in mediating leptin resistance. In addition,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1670
更新日期:2012-03-01 00:00:00
abstract::The expression and functions of receptors for the beta-chemokine, regulated upon activation, normal T cell expressed, and secreted (RANTES)/CCL5, were investigated in osteoblasts. Both primary osteoblasts and the MC3T3-E1 osteoblast cell line express the RANTES receptors, CCR1, 3, 4, and 5 (by RT-PCR), which encode fu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0065
更新日期:2005-05-01 00:00:00
abstract::As human pregnancy advances, CRH increases exponentially and is hypothesized to trigger the transition from myometrial quiescence to active contractions at labor. Paradoxically, CRH stimulates cAMP production, suggesting it should cause relaxation. To evaluate CRH as a mediator of quiescence, the effect of CRH on cont...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0348
更新日期:2009-12-01 00:00:00
abstract::The present study examined the effects of FSH and insulin (IN) on 17 beta-estradiol (E2) secretion and granulosa cell proliferation. For these studies, immature rat ovaries were maintained in perifusion culture and continuously exposed to FSH (0-800 ng RP-1 eq/ml) and /or IN (0.2 U/ml). At specific times, the ovaries ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-191
更新日期:1991-01-01 00:00:00
abstract::The neuroendocrine hormones ACTH and corticotropin- releasing factor (CRF), which are involved in the stress response, have acute effects on arterial pressure. New evidence indicates that urocortin (UCN), the putative agonist for the CRF type 2 receptor, has selective cardiovascular actions. The responses to long-term...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0432
更新日期:2004-12-01 00:00:00
abstract::Mechanisms underlying lymphocyte infiltration of the thyroid gland and orbit in Graves' disease (GD) are poorly understood. The IGF-I receptor (IGF-IR) is a newly recognized self-antigen that, when activated in GD fibroblasts by IGF-I or GD-IgGs, provokes the expression of IL-16 and RANTES (regulated upon activation, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1375
更新日期:2006-04-01 00:00:00
abstract::Important enzymes for thyroid hormone metabolism, antioxidative defense, and intracellular redox control contain selenocysteine (Sec) in their active centers. Expression of these selenoproteins is tightly controlled, and a sex-specific phenotype is observed on disturbance of selenium (Se) transport in mice. Therefore,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0689
更新日期:2006-12-01 00:00:00
abstract::The importance of monoaminergic mechanisms for the regulation of GH-releasing factor (GRF)-stimulated GH secretion was studied in perifused rat anterior pituitaries. Dopamine (greater than 1 microL) reduced GRF-stimulated GH release, but 5-hydroxytryptamine (5-HT; 1 mL) had no effect. The substrates for L-aromatic ami...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-2-780
更新日期:1987-02-01 00:00:00
abstract::We recently identified a 32 K mol wt insulin-like growth factor (IGF)-binding protein (BP) which is markedly increased in the serum of streptozotocin-diabetic rats and recognized by antiserum against the human amniotic fluid IGFBP (hIGFBP-1). In the present study we sought to confirm that this protein represents the r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-789
更新日期:1990-08-01 00:00:00
abstract::Cultured pineal ependymal cells from rat fetuses aged 17 to 19 days post-coitum release into their media a substance that has hydroosmotic, antidiuretic and rat uterine activities. Paper chromatography of concentrates from culture media demonstrates that the substance possessing all the above activities was eluted fro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-1-205
更新日期:1977-01-01 00:00:00
abstract::The pharmacokinetics of TRH and its metabolite deamido-TRH (TRH-OH) were evaluated and compared. After a 60- to 90-min infusion of the appropriate peptide into rats, the MCR, half-life of disappearance (t 1/2), and volume of distribution were calculated. The MCR of TRH averaged 4 ml/min, whereas that of TRH-OH was 6.5...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-3-1031
更新日期:1984-09-01 00:00:00
abstract::Steroid hormones can differentially modify gonadotropin release stimulated by GnRH. Decreased GnRH-mediated gonadotropin release has been observed in vitro after pretreatment with androgens or glucocorticoids. In this study, we tested this phenomenon further with the use of a new cytochemical stain for a potent biotin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-1-396
更新日期:1985-07-01 00:00:00
abstract::Regulation of MUC1 expression and removal is a salient feature of embryo implantation, bacterial clearance, and tumor progression. In some species, embryo implantation is accompanied by a transcriptional decline in uterine epithelial expression of MUC1. In other species, MUC1 is locally removed at blastocyst attachmen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0399
更新日期:2004-09-01 00:00:00
abstract::Sterol regulatory element binding protein 1c (SREBP1c) is a master regulator of lipogenic gene expression in liver and adipose tissue, where its expression is regulated by a heterodimer of nuclear receptor-type transcription factors retinoid X receptor-alpha (RXRalpha) and liver X receptor-alpha (LXRalpha). Despite th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1461
更新日期:2008-05-01 00:00:00
abstract::The role of adrenergic stimulation in the regulation of mitogen-activated protein kinase (MAPK) in rat pinealocytes was investigated by measuring phosphorylated MAPK using Western blot analysis and a MAPK enzymatic assay. Stimulation with the endogenous neurotransmitter, norepinephrine (NE; a mixed alpha- and beta-adr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.12.7856
更新日期:2000-12-01 00:00:00
abstract::Recent studies of the human brain insulin receptor raise the possibility that some of the differences described between rat brain insulin receptors and rat liver insulin receptors may have been due to the presence of insulin-like growth factor I (IGF-I) receptors in the brain. This study details side by side compariso...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-1933
更新日期:1988-05-01 00:00:00
abstract::Fat intake has long been associated with the development of obesity. The studies described herein show that fat adversely affects adipocyte adrenergic receptor (AR) expression and function. As beta 3AR agonists have been shown to acutely reduce adipose tissue mass and improve thermogenesis in genetically obese rodents...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.1.4829
更新日期:1997-01-01 00:00:00
abstract::Myocardial fibrosis is a key pathological change in a variety of heart diseases contributing to the development of heart failure, arrhythmias, and sudden death. Recent studies have shown that relaxin prevents and reverses cardiac fibrosis. Endogenous expression of relaxin was elevated in the setting of heart disease; ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1114
更新日期:2012-08-01 00:00:00
abstract::The purpose of these experiments was to determine if the powerful effect of sodium chloride concentration on angiotensin II- and potassium-stimulated aldosterone secretion by isolated perfused adrenal glands is mediated by the sodium or chloride ion or by the obligatory change in osmolality. We used isolated Ringer's ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-4-1621
更新日期:1985-04-01 00:00:00
abstract::We and others have recently shown that 1alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] significantly inhibits cell proliferation and increases secretion of prostate-specific antigen (PSA) in LNCaP cells, an androgen-responsive human prostate cancer cell line. The present study was designed to investigate the possible int...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.8.5328
更新日期:1997-08-01 00:00:00
abstract::Retinoid X receptor (RXR) occupies a central position within the nuclear receptor superfamily, serving as an obligatory partner to numerous other nuclear receptors, including vitamin D receptor (VDR). In the current study, we examined whether phosphorylation of RXRα at serine 260 affects VDR/RXR and VDR interacting pr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03098
更新日期:2018-03-01 00:00:00
abstract::Male Sprague-Dawley rats were infused continuously for 13 days with vehicle or 75 pmol (31.2 ng)/day 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] by means of Alzet osmotic minipumps implanted s.c. Animals infused with 1,25-(OH)2D3 exhibited mild hypercalcemia (11.2 vs. 10.2 mg/dl in controls), a 136% increase in the serum ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-6-2580
更新日期:1986-12-01 00:00:00
abstract::The growth regulatory activity of transforming growth factor-beta 1 (TGF beta 1) was studied in a clonal strain of thyroid papillary carcinoma cell (NPA). Despite the presence of TGF beta 1 and its receptor messenger RNA in thyroid carcinoma, the molecular mechanism of TGF beta 1 action on cell growth of thyroid carci...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.4.7925100
更新日期:1994-10-01 00:00:00
abstract::Poor growth before birth is associated with impaired insulin sensitivity later in life, increasing the risk of type 2 diabetes. The tissue sites at which insulin resistance first develops after intrauterine growth restriction (IUGR), and its molecular basis, are unclear. We have therefore characterized the effects of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1955
更新日期:2012-05-01 00:00:00
abstract::Attempts to assess human placental GH variant (hGH-V) and chorionic somatomammotropin (hCS) RNA in choriocarcinoma cell lines have been hampered by low levels of expression and limited sensitivity of RNA blotting analysis. We examined human choriocarcinoma BeWo, JAR, and JEG-3 cell lines as well as samples of complete...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.6.7515000
更新日期:1994-06-01 00:00:00
abstract::In vitro antiglucocorticoids (cortexolone and progesterone) were evaluated as in vivo antagonists of dexamethasone-induced increases in liver tyrosine amino transferase (TAT; EC 2.6.1.5), tryptophan oxygenase (TPO; EC 1.13.1.12), and glycogen deposition. Cortexolone antagonized the TPO and glycogen responses to dexame...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-1-117
更新日期:1980-07-01 00:00:00
abstract::The effect of progesterone on the secretion of LH was studied in intact and castrated immature female sheep between 12 and 42 weeks of age. (First spontaneous ovulation occurs between 30 and 50 weeks of age.) Progesterone was administered by means of Silastic capsules inserted SC to achieve circulating progesterone co...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-1-1
更新日期:1976-07-01 00:00:00