Abstract：
:A method for the global analysis of yeast intracellular metabolites, based on electrospray mass spectrometry (ES-MS), has been developed. This has involved the optimization of methods for quenching metabolism in Saccharomyces cerevisiae and extracting the metabolites for analysis by positive-ion electrospray mass spectrometry. The influence of cultivation conditions, sampling, quenching and extraction conditions, concentration step, and storage have all been studied and adapted to allow direct infusion of samples into the mass spectrometer and the acquisition of metabolic profiles with simultaneous detection of more than 25 intracellular metabolites. The method, which can be applied to other micro-organisms and biological systems, may be used for comparative analysis and screening of metabolite profiles of yeast strains and mutants under controlled conditions in order to elucidate gene function via metabolomics. Examples of the application of this analytical strategy to specific yeast strains and single-ORF yeast deletion mutants generated through the EUROFAN programme are presented.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Castrillo JI,Hayes A,Mohammed S,Gaskell SJ,Oliver SGdoi
10.1016/s0031-9422(02)00713-6subject
Has Abstractpub_date
2003-03-01 00:00:00pages
929-37issue
6eissn
0031-9422issn
1873-3700pii
S0031942202007136journal_volume
62pub_type
杂志文章相关文献
PHYTOCHEMISTRY文献大全abstract：:Black aspergilli are distributed worldwide and represent one of the most prolific sources of metabolites with biomedical and agrochemical interests. However, due to their similar morphological characteristics and insufficient molecular identification, the taxonomic classification of black aspergilli remains ill-define...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2020.112399
更新日期：2020-08-01 00:00:00
abstract：:Six phragmalin limonoids, named tabulalin and tabulalides A-E, were isolated from the root bark of Chukrasia tabularis (Meliaceae). Their structures were determined by spectroscopic methods, and their antifeedant properties evaluated. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.08.010
更新日期：2004-10-01 00:00:00
abstract：:During the search for new protein tyrosine phosphatase (PTP) 1B inhibitors, EtOH extracts from the aerial parts of Lantana camara L. (lantana) collected at Manado (Indonesia) and two subtropical islands in Japan (Ishigaki and Iriomote Islands, Okinawa) exhibited potent inhibitory activities against PTP1B in an enzyme ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2017.08.020
更新日期：2017-12-01 00:00:00
abstract：:From the hexane extract of berries of Juniperus excelsa, one new and four known diterpenes were isolated besides a known sesquiterpene. The structures of the known diterpenes were identified as isopimaric, isocommunic, (-)ent-trans communic and sandracopimaric acids, along with the sesquiterpene 4a-hydroxycedrol and t...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(98)00675-x
更新日期：1999-04-01 00:00:00
abstract：:The heterologous expression of cDNAs encoding the alkaloid biosynthetic enzymes, strictosidine synthase [EC 4.3.3.2] from Rauvolfia serpentina and the berberine bridge enzyme [(S)-reticuline: oxygen oxidoreductase (methylene-bridge-forming), EC 1.5.3.9] from Eschscholtzia californica, has been achieved in a cell cultu...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)94763-0
更新日期：1994-01-01 00:00:00
abstract：:The incubation of 15α-hydroxy-ent-kaur-9(11),16-dien-19-oic acid (15α-hydroxy-grandiflorenic acid) with the fungus Fusarium fujikuroi gave as main metabolite its 3β,6β-dihydroxy derivative, which by an oxidative decarboxylation afforded a 19-nor compound with a 4,18-double bond. Other substances obtained were a 3α-hyd...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2013.01.006
更新日期：2013-05-01 00:00:00
abstract：:Two rare cadinane-type sesquiterpenes, lyophyllone A (1) and lyophyllanetriol A (2), were isolated from the mushroom Lyophyllum transforme. The structures were elucidated on the basis of exhaustive NMR techniques, together with MS, UV-Vis and molecular modelling. The absolute configuration of lyophyllone A was determi...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2013.03.019
更新日期：2013-09-01 00:00:00
abstract：:Eleven oleanane-type triterpenoid saponins, foegraecumosides A-K, and eight known ones, were isolated from the aerial parts of Lysimachia foenum-graecum. Their structures were elucidated by spectroscopic data analyses and chemical methods. All isolated saponins were evaluated for their cytotoxicity against four human ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2017.01.021
更新日期：2017-04-01 00:00:00
abstract：:Three undescribed isoflavones, derriscandenon A, B, and C, together with seven known isoflavones were isolated and structurally characterized during a study of the chemical constituents in the leaves of Derris scandens (Roxb.) Benth (Leguminosae, Fabaceae) collected in Bangladesh. The inhibitory activity of the compou...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2020.112376
更新日期：2020-07-01 00:00:00
abstract：:The leaves of Cyclocarya paliurus with sweet taste are often used as herbal tea in People's Republic of China. In this study eight previously undescribed seco-dammarane type triterpenoids, cyclocariols A-H along with seven known compounds were isolated and characterized from its leaves. A possible biogenetic pathway f...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2017.10.013
更新日期：2018-01-01 00:00:00
abstract：:Two red Cystofilobasidium spp. isolated from spring sap-flows of Betula pendula were analysed for their carotenoid content. In Cystofilobasidium infirmominiatum, three unusual pigments were detected and identified by structure elucidation as oxidised torulene derivatives. These included 16'-hydroxytorulene and torular...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2007.05.019
更新日期：2007-10-01 00:00:00
abstract：:The cell extract of a cultured terrestrial Nostoc sp. (UIC 10062), obtained from a sample collected at Grand Mere State Park in Michigan, displayed antiproliferative activity against the HT-29 human colon cancer cell line. Bioactivity-guided fractionation of the cell extract, combined with LC-MS analysis, led to the i...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2012.03.005
更新日期：2012-07-01 00:00:00
abstract：:A single-chain variable antibody fragment (scFv) library tested against the non-structural NSP5 protein of human rotavirus A was screened by a yeast two-hybrid system against three proteins derived from the RNA-dependent RNA polymerase (RdRp) of cucumber mosaic virus (CMV), with the aim of blocking their function and ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2019.03.006
更新日期：2019-06-01 00:00:00
abstract：:Eight previously undescribed ditepenoids, including four oxygenated abietanes (roscotanes A-D) and four oxygenated pimaranes (roscoranes A-D), along with twelve known diterpenoids were isolated from the whole plants of Kaempferia roscoeana. Their structures were elucidated by extensive spectroscopic analysis, and the ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2017.07.008
更新日期：2017-11-01 00:00:00
abstract：:Erucastrum canariense Webb & Berthel. (Brassicaceae) is a wild crucifer that grows in rocky soils, in salt and water stressed habitats, namely in the Canary Islands and similar environments. Abiotic stress induced by copper chloride triggered formation of a phytoalexin and galacto-oxylipins in E. canariense, whereas w...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2017.03.005
更新日期：2017-07-01 00:00:00
abstract：:From the mesocarp of Balanites aegyptiaca fruits, two pregnane glycosides were isolated. One is new and identified as pregn-5-ene-3 beta,16 beta,20(R)-triol 3-O-(2,6-di-O-alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside (balagyptin), while the other is known and assigned as pregn-5-ene-3 beta,16 beta,20(R)-triol 3-O-be...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(95)00551-h
更新日期：1995-12-01 00:00:00
abstract：:The flavonoid rich grain of buckwheat (Fagopyrum esculentum Moench, Fam. Polygonaceae) is of high nutritional value. With the aim to improve its agronomic productivity, cultivars were crossed with the wild species F. homotropicum which, however, differs in its flavonoid content. The intention of this work was to deter...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2008.01.001
更新日期：2008-04-01 00:00:00
abstract：:Two new triterpenoid glycosides, quinovic acid-3 beta-O-beta-D- glucopyranosyl-(1-->4)-alpha-L-rhamnopyranoside and quinovic acid-3 beta-O-beta-D-glucopyranosyl-(1-->3)-beta-D-fucopyranoside (named rubelloside A and B, respectively), were isolated from roots of Adina rubella. Their structures were elucidated by spectr...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(95)00119-r
更新日期：1995-07-01 00:00:00
abstract：:The unusual chlorinated coumarinolignan, 5-chloropropacin, along with several other known compounds have been isolated from the roots of Mondia whitei. The structure of the chlorinated coumarinolignan was determined by NMR and mass spectroscopic analyses. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.11.012
更新日期：2005-03-01 00:00:00
abstract：:The kaempferol derivative 3,7-di-O-methyl-4'-O-beta-[alpha rhamnosyl (1 --> 6)]-glucopyranoside (siparunoside) was isolated from the leaves of Sparuna apiosyce. Its structure was established by extensive NMR studies. The alkaloids reticuline and liriodenine were also isolated from the leaves along with the kaempferol ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00222-3
更新日期：2000-11-01 00:00:00
abstract：:A phytochemical investigation of the roots of Symplocos caudata Wall (Symplocaceae) resulted in isolation and characterization of four optical isomers of a neolignan glycoside (1-4), a lignan lactone glycoside (5), a phenylpropanoid glycoside (6), as well as two known compounds (7, 8). Their structures were elucidated...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2007.08.022
更新日期：2008-02-01 00:00:00
abstract：:Three fructokinase isozymes (FKI, FKII, FKIII) were separated from both immature and ripe tomato fruit pericarp. All three isozymes were specific for fructose with undetectable activity towards glucose or mannose. The three isozymes could be distinguished from one another with respect to response to fructose, Mg and n...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(01)00331-4
更新日期：2001-11-01 00:00:00
abstract：:Bacterial LPS have the ability to act as modulators of the innate immune response in plants. Complex and largely unresolved perception systems exist for LPS on the plant cell surfaces that lead to the activation of multiple intracellular defense signaling pathways. The aim of the present study was to investigate the p...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.09.005
更新日期：2004-11-01 00:00:00
abstract：:Non-targeted LC-MS metabolomics on fruit of three wild and domesticated apple species (Malus sylvestris, M. sieversii and M. domestica) showed that two crab apple (M. sylvestris) accessions were distinguished by high concentrations of an ascorbic acid glycoside (AAG). This was partly purified, but key NMR signals were...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2020.112297
更新日期：2020-05-01 00:00:00
abstract：:Petunia hybrida and Citrus paradisi have significantly different flavonoid accumulation patterns. Petunia sp. tend to accumulate flavonol glycosides and anthocyanins while Citrus paradisi is known for its accumulation of flavanone diglycosides. One possible point of regulation of flavanone metabolism is flavanone 3-hy...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(03)00341-8
更新日期：2003-09-01 00:00:00
abstract：:The effect of light exposure on the steroidal glycoalkaloid content of Solanum phureja tubers has been investigated and compared with that in domesticated potato (Solanum tuberosum) tubers. The results indicated that the increase in the concentration of solanidine-based glycoalkaloids, alpha-solanine and alpha-chaconi...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00023-6
更新日期：2000-04-01 00:00:00
abstract：:Toddaliopsins A-D, four novel 1,2,3-trioxygenated acridone alkaloids, have been isolated from the leaves of Toddaliopsis bremekampii. Toddaliopsins B-D are the first reported acridone alkaloids with substituted N-methyl groups, in the light of which the chemotaxonomic relationship of Toddaliopsis and Vepris is discuss...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2005.04.036
更新日期：2005-07-01 00:00:00
abstract：:Evidence is presented for the presence of xylogalacturonan (XGA) in Arabidopsis thaliana. This evidence was obtained by extraction of pectin from the seeds, root, stem, young leaves and mature leaves of A. thaliana, followed by treatment of these pectin extracts with xylogalacturonan hydrolase (XGH). Upon enzymatic tr...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2007.01.016
更新日期：2007-04-01 00:00:00
abstract：:While the myrosinase-glucosinolate system has been reviewed in recent years by a number of authors, little attention has been paid to the enzymic and non-enzymic degradation of glucosinolates. Non-enzymic degradation processes are particularly important in the processing of brassica vegetables with respect to both fla...
journal_title：Phytochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.phytochem.2006.02.024
更新日期：2006-06-01 00:00:00
abstract：:Two biflavonoids namely, 2",3"-dihydroochnaflavone and 2",3"-dihydroochnaflavone 7"-O-methyl ether, and a flavonoid glycoside, 6-gamma,gamma-dimethylallyltaxifolin 7-O-beta-D-glucoside were isolated from the leaves of Ochna integerrima. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00409-x
更新日期：2001-02-01 00:00:00
