Abstract:
:Three fructokinase isozymes (FKI, FKII, FKIII) were separated from both immature and ripe tomato fruit pericarp. All three isozymes were specific for fructose with undetectable activity towards glucose or mannose. The three isozymes could be distinguished from one another with respect to response to fructose, Mg and nucleotide donor concentrations and this allowed the comparison of the fruit enzymes with the gene products of the two known cloned tomato fructokinase genes, LeFRK1 and LeFRK2. FKI was characterized by both substrate (fructose), as well as Mg, inhibition; FKII was inhibited by neither fructose nor Mg; and FKIII was inhibited by fructose but not by Mg. ATP was the preferred nucleotide donor for all three FKs and FKI showed inhibition by CTP and GTP above 1 mM. All three FKs showed competitive inhibition by ADP. During the maturation of the tomato fruit total FK activity decreased dramatically. There were decreases in activity of all three FKs, nevertheless, all were still observed in the ripe fruit. The two tomato LeFRK genes were expressed in yeast and the gene products were characterized with respect to the distinguishing characteristics of fructose, Mg and nucleotide inhibition. Our results indicate that FKI is the gene product of LeFRK2 and FKII is probably the gene product of LeFRK1.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Petreikov M,Dai N,Granot D,Schaffer AAdoi
10.1016/s0031-9422(01)00331-4subject
Has Abstractpub_date
2001-11-01 00:00:00pages
841-7issue
6eissn
0031-9422issn
1873-3700pii
S0031-9422(01)00331-4journal_volume
58pub_type
杂志文章相关文献
PHYTOCHEMISTRY文献大全abstract::Phytochemical investigation of the roots and aerial parts of Andrographis paniculata Nees yielded a new flavone, 5-hydroxy-7,2',6'-trimethoxyflavone and an unusual 23-carbon terpenoid, 14-deoxy-15-isopropylidene-11,12-didehydroandrographolide together with five known flavonoids and four known diterpenoids. The structu...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(03)00051-7
更新日期:2003-04-01 00:00:00
abstract::Poliumoside, forsythoside B, echinacoside and arenarioside, caffeic glycoside esters, were isolated from several species of Oleaceae. The poliumoside/forsythoside ratio distinguishes Jasminum nudiflorum from J. mesnyi. Arenarioside and forsythoside B act in Forsythia species as good hybridization markers. ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/0031-9422(92)80032-a
更新日期:1992-03-01 00:00:00
abstract::Exhaustive chromatographic separation of the chloroform portion of the ethanolic extract obtained from Paulownia tomentosa (Thunb). Steud. (Paulowniaceae) fruits has led to isolation of ten C-6 geranylated flavanones tomentodiplacone C-I and mimulone C-E, featured by 3'-methoxy and 4'-hydroxy or 4'-hydroxy substitutio...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2013.01.002
更新日期:2013-05-01 00:00:00
abstract::A novel minor triterpenoid saponin mimusin (3-O-[beta-D-glucopyranosyl- (1-->6)-beta-D-glucopyranosyl]-2 beta,3 beta,6 beta,23-tetrahydroxyolean- 12-en-28-oic acid 28-O-alpha-L-rhamnopyranosyl-(1-->3)-beta-D-xylopyranosyl-(1-->4)-alpha- L- rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranoside was isolated from the seeds o...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(96)00659-0
更新日期:1997-03-01 00:00:00
abstract::The cuticle of higher plants functions primarily as a protective barrier for the leaves and fruits, controlling microbial attack as well as the diffusion of water and chemicals from the outside environment. Its major chemical constituents are waxes (for waterproofing) and cutin (a structural support polymer). However,...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(01)00106-6
更新日期:2001-07-01 00:00:00
abstract::Acylation is a prevalent chemical modification that to a significant extent accounts for the tremendous diversity of plant metabolites. To catalyze acyl transfer reactions, higher plants have evolved acyltransferases that accept beta-acetal esters, typically 1-O-glucose esters, as an alternative to the ubiquitously oc...
journal_title:Phytochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.phytochem.2009.07.023
更新日期:2009-10-01 00:00:00
abstract::Extracts of carboxylic acids from immature fruits of strawberry (Fragaria x ananassa Duch. cv. Elsanta) were analysed for gibberellins by combined gas chromatography-mass spectrometry. The following previously characterised gibberellins were identified by comparison of their mass spectra and Kovats retention indices (...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(00)00237-5
更新日期:2000-12-01 00:00:00
abstract::Four dimeric chalcone derivatives, 8″,9″-dihydrowelwitschin H, uvarins A-C, a naphthalene derivative, 2-hydroxy-3-methoxy-6-(4'- hydroxyphenyl)naphthalene, and the known dimeric chalcones, dependensin and welwitschin E, flavonoids, a cyclohexane oxide derivative, an aromatic aldehyde were isolated from the roots of Uv...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2016.12.009
更新日期:2017-03-01 00:00:00
abstract::Two biflavonoids namely, 2",3"-dihydroochnaflavone and 2",3"-dihydroochnaflavone 7"-O-methyl ether, and a flavonoid glycoside, 6-gamma,gamma-dimethylallyltaxifolin 7-O-beta-D-glucoside were isolated from the leaves of Ochna integerrima. ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(00)00409-x
更新日期:2001-02-01 00:00:00
abstract::Triterpenoid saponins, impatienosides A-G, together with 12 known saponins, were isolated from the whole plants of Impatiens siculifer. Their structures were established on the basis of extensive 1D and 2D NMR and MS analyses coupled with chemical degradation. Cytotoxic activities of the isolated saponins were evaluat...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2009.03.022
更新日期:2009-04-01 00:00:00
abstract::From the roots of Bupleurum wenchuanense 14 derivatives of saikosaponin were isolated and identified as 2"-O-beta-D-xylopyranosylsaikosaponin b2, 3",6"-O O-diacetylsaikosaponin b2, 2"-O-beta-D-glucopyranosylsaikosaponin b2, saikosaponin b2, 6"-O-acetylsaikosaponin b2, saikosaponin d, 2"-O-acetylsaikosaponin d, 3"-O-ac...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/0031-9422(93)85049-w
更新日期:1993-07-01 00:00:00
abstract::The flavonoid profiles of two monotypic genera, Teucridium and Tripora, have been studied by analytical methods. These genera were formerly placed in the Verbenaceae, but are now classified in the Lamiaceae, subfamily Ajugoideae. The major flavonoids of both genera were identified as glycosides of scutellarein 4'-meth...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(02)00192-9
更新日期:2002-08-01 00:00:00
abstract::The twigs of Dorstenia prorepens furnished the digeranylated chalcone, 5,3'-(3,7-dimethyl-2,6-octadienyl)-3,4, 2',4'-tetrahydroxychalcone while Dorstenia zenkeri yielded the 3',4'-(3-hydroxy-2,2-dimethyldihydropyrano)-4,2'-dihydroxychalcone and a bichalcone. 4-Hydroxylonchocarpin was found in both plants. D. prorepens...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(01)00483-6
更新日期:2002-04-01 00:00:00
abstract::Although the mechanism of accumulation of C8-C16 saturated fatty acids in seed oils has been well-studied, the control of stearic (C18:0) acid deposition in high stearate seed fat is still unclear. We investigated the mechanism that regulates high level of stearate and oleate (C18:1) accumulation in mango (Mangifera i...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2010.11.004
更新日期:2011-02-01 00:00:00
abstract::The kaempferol derivative 3,7-di-O-methyl-4'-O-beta-[alpha rhamnosyl (1 --> 6)]-glucopyranoside (siparunoside) was isolated from the leaves of Sparuna apiosyce. Its structure was established by extensive NMR studies. The alkaloids reticuline and liriodenine were also isolated from the leaves along with the kaempferol ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(00)00222-3
更新日期:2000-11-01 00:00:00
abstract::Along with three known drimanes, polygodial. 1-beta-(p-methoxycinnamoyl) polygodial and mukaadial, the sesquiterpene drimane named drimanial was isolated from the bark of Drimys winteri (Winteraceae). Its structure was elucidated based on spectroscopic evidence. Drimanial exhibited antinociceptive action against aceti...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(00)00515-x
更新日期:2001-05-01 00:00:00
abstract::Prenylation reactions contribute considerably to the diversity of natural products. Polyprenylated secondary metabolites include hyperforin which is both quantitatively and pharmacologically a major constituent of the medicinal plant Hypericum perforatum (St. John's wort). Cell cultures of the related species Hypericu...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2004.10.020
更新日期:2005-01-01 00:00:00
abstract::The genuine major benzylisoquinoline alkaloid occurring in the traditional medicinal plant greater celandine (Chelidonium majus L.) is 13,14-dihydrocoptisine and not - as described previously - coptisine. Structure of 13,14-dihydrocoptisine was elucidated. The discrepancy between the alkaloid pattern of the living pla...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2015.01.006
更新日期:2015-03-01 00:00:00
abstract::ent-19-Hydroxykaur-16-en-15-one is transformed to ent-3 beta,16 beta,19-trihydroxykauran-15-one by Cephalosporium aphidicola. The structure of this metabolite was established by X-ray crystallography. ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/0031-9422(94)85065-8
更新日期:1994-09-01 00:00:00
abstract::The chemical structures, syntheses, metabolism and biological activities of the cruciferous phytoalexins discovered to date, with particular focus on the latest results dealing with their biosynthesis and detoxification are reviewed. ...
journal_title:Phytochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.phytochem.2010.03.020
更新日期:2010-08-01 00:00:00
abstract::A protein phosphatase (WbPP) has been purified from the soluble fraction of the winged bean (Psophocarpus tetragonolobus) shoot extract. The preparation is essentially homogenous as shown by the constant specific activity of the enzyme across the peak fractions, eluted from the thiophosphorylated histone-Sepharose aff...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(98)00473-7
更新日期:1999-09-01 00:00:00
abstract::Repeated fractionations of the methanol extract of the subterranean parts (rhizomes and roots) of Gentiana siphonantha afforded two new and five known secoiridoids, in addition to the widespread plant constituents beta-sitiosterol, daucosterol and oleanolic acid. The structures of the new acyl secoiridoid glycosides w...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(97)00225-2
更新日期:1997-11-01 00:00:00
abstract::Sorghum SbSTS1, a pathogen inducible gene, was previously demonstrated to encode an enzyme with stilbene synthase activity. In this study, we attempt to identify the stilbene derivatives that accumulate in infected sorghum seedlings after inoculation with the anthracnose pathogen Colletotrichum sublineolum. Scanning f...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2007.09.012
更新日期:2008-02-01 00:00:00
abstract::All stereoisomers of methyl jasmonate (MJA) were prepared, and their effects on cell yield and promotion of paclitaxel (Taxol) and baccatin III production investigated in cell suspension cultures of Taxus media. (3R,7S)-MJA showed the strongest cell growth inhibition, followed by (3R,7R)-MJA. In contrast, (3S,7R)- and...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(00)00006-6
更新日期:2000-05-01 00:00:00
abstract::A single-chain variable antibody fragment (scFv) library tested against the non-structural NSP5 protein of human rotavirus A was screened by a yeast two-hybrid system against three proteins derived from the RNA-dependent RNA polymerase (RdRp) of cucumber mosaic virus (CMV), with the aim of blocking their function and ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2019.03.006
更新日期:2019-06-01 00:00:00
abstract::3β-Hydroxy-5β-cholestan-6-one was identified in the EtOAc extract of Ajuga hairy roots by micro-analysis using LC-MS/MS in the multiple reaction mode (MRM). Furthermore, administration of (2,2,4,4,7,7-(2)H6)- and (2,2,4,4,6,7,7-(2)H7)-cholesterols to the hairy roots followed by LC-MS/MS analysis of the EtOAc extract o...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2014.12.019
更新日期:2015-03-01 00:00:00
abstract::Seven pure flavonoids were isolated and identified from five moss species. The flavonoids were the flavones apigenin, apigenin-7-O-triglycoside, lucenin-2, luteolin-7-O-neohesperidoside, saponarine and vitexin; and the biflavonoid bartramiaflavone. Some of these flavonoids were shown to have pronounced antibacterial e...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(99)00286-1
更新日期:1999-12-01 00:00:00
abstract::Small molecules (xenobiotics) that inhibit cell-wall-localised enzymes are valuable for elucidating the enzymes' biological roles. We applied a high-throughput fluorescent dot-blot screen to search for inhibitors of Petroselinum xyloglucan endotransglucosylase (XET) activity in vitro. Of 4216 xenobiotics tested, with ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2015.06.016
更新日期:2015-09-01 00:00:00
abstract::Chemical investigation of the traditional Chinese medicine, Murraya kwangsiensis, led to the isolation of 16 undescribed biscarbazole alkaloids, kwangsines A-M, two undescribed natural products, (+/-)-bispyrayafoline C, and 19 known monomeric analogues. (±)-Bispyrayafoline C and (±)-kwangsines A-C are four pairs of bi...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2019.112186
更新日期:2020-02-01 00:00:00
abstract::The fatty acid compositions of 22 species of marine macrophytes, belonging to the Ceramiales, Cryptonemiales, Nemalionales, Laminariales, Chordariales, Scytosiphonales, Desmarestiales, Dictyosiphonales, Fucales, Dictyotales and Ulvales and collected from the Bohai Sea, were determined by capillary gas chromatography. ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(01)00437-x
更新日期:2002-01-01 00:00:00