Abstract：
:A protein phosphatase (WbPP) has been purified from the soluble fraction of the winged bean (Psophocarpus tetragonolobus) shoot extract. The preparation is essentially homogenous as shown by the constant specific activity of the enzyme across the peak fractions, eluted from the thiophosphorylated histone-Sepharose affinity column, the last step of purification and by single protein bands on polyacrylamide gel electrophoresis (PAGE) in the presence as well as absence of denaturating agents. The monomeric nature of WbPP is revealed by an M(r) of 92,000 and 85,000, respectively, as estimated by SDS-PAGE and gel permeation chromatography under non-denaturating conditions. Autophosphorylated calmodulin-like domain protein kinase (P-WbCDPKI) [Saha, P., & Singh, M. (1995). Biochem. J., 305, 205] and phosphohistone H1 (P-hisH1), prepared by using the other homologous CDPK, i.e. WbCDPKII [Ganguly, S., & Singh, M. (1998). Phytochemistry, 48(1), 61], are good substrates of the purified enzyme, while P-hisH1 and phosphocasein prepared by using heterologous cAMP-dependent protein kinase, are respectively very poor and totally inactive as substrate. WbPP is adjudged to be a protein phosphoserine phosphatase since phosphoserine is the only phosphorylated amino acid residue detected in our earlier analysis of P-WbCDPKI and P-hisH1. The enzyme is strongly stimulated by a combination of Mg2+ and Ca2+, without being dependent on either of them and is also unaffected by calmodulin and fluphenazine. Orthovanadate strongly inhibits the enzyme while okadaic acid is a poor inhibitor.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Ganguly S,Singh Mdoi
10.1016/s0031-9422(98)00473-7subject
Has Abstractpub_date
1999-09-01 00:00:00pages
239-46issue
2eissn
0031-9422issn
1873-3700pii
S0031-9422(98)00473-7journal_volume
52pub_type
杂志文章相关文献
PHYTOCHEMISTRY文献大全abstract：:Cytochrome P450-dependent monooxygenases (P450s) belonging to the CYP93E subfamily catalyze the C-24 oxidation of the triterpene backbone during the biosynthesis of triterpenoid saponins, which are bioactive plant natural products. In our attempts to produce plant triterpenoids in the yeast Saccharomyces cerevisiae, w...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2014.10.002
更新日期：2014-12-01 00:00:00
abstract：:Vanadium-dependent peroxidase activity in extracts of Ascophyllum nodosum growing in the intertidal region close to Roscoff/France, and algal vanadium levels, followed approximately similar seasonal variation, as deduced from a study lasting from April 2005 to March 2006. High peroxidase (PO) activity was found in ext...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2008.09.004
更新日期：2008-11-01 00:00:00
abstract：:Among the four cold shock domain proteins (CSDPs) identified in Arabidopsis thaliana, it has recently been shown that CSDP1 harboring seven CCHC-type zinc fingers, but not CSDP2 harboring two CCHC-type zinc fingers, function as a RNA chaperone during cold adaptation. However, the structural features relevant to this d...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2009.12.006
更新日期：2010-04-01 00:00:00
abstract：:Three phenolic glycosides 5-O-{[5''-O-E-(4'''-O-threo-guaiacylglycerol)-feruloyl]-beta-apiofuranosyl-(1-->2)-beta-xylopyranosyl} gentisic acid, 5-O-[(5''-O-vanilloyl)-beta-apiofuranosyl-(1-->2)-beta-xylopyranosyl] gentisic acid and 1-O-[E-(4'''-O-threo-guaiacylglycerol)-feruloyl]-3-O-beta-galacturonopyranosyl glycerol...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2009.07.021
更新日期：2009-07-01 00:00:00
abstract：:Stereumins Q-U, together with known stereumins A, B, K, L, and N, as well as ent-strobilols E and G were isolated from the culture of Stereum cf. sanguinolentum BCC 22926. Their structures were elucidated by extensive spectroscopic analyses. The absolute configurations of stereumins A and Q, as well as ent-strobilol E...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2014.06.006
更新日期：2014-09-01 00:00:00
abstract：:Phenolic composition of Ambrosia artemisiifolia L. pollen and sub-pollen particles (SPP) aqueous extracts was determined, using a novel extraction procedure. Total phenolic and flavonoid content was determined, as well as the antioxidative properties of the extract. Main components of water-soluble pollen phenolics ar...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2014.10.022
更新日期：2015-01-01 00:00:00
abstract：:Salicinoids are phenolic glycosides (PGs) characteristic of the Salicaceae and are known defenses against insect herbivory. Common examples are salicin, salicortin, tremuloidin, and tremulacin, which accumulate to high concentrations in the leaves and bark of willows and poplars. Although their biosynthetic pathway is...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2014.10.018
更新日期：2015-05-01 00:00:00
abstract：:An investigation of an ethyl acetate extract of Embelia schimperi leaves has led to the isolation of 10 oleanane-type triterpenes characterized as 3beta,16alpha-di-O-acetyl-13beta, 28-epoxyoleanane (1), 3beta-acetyl-16-oxo-13beta, 28-epoxyoleanane (2), 3beta-acetyl-16alpha-hydroxy-13beta, 28-epoxyoleanane (3), 3beta-a...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2006.07.025
更新日期：2006-12-01 00:00:00
abstract：:Nine undescribed compounds, including five xanthone derivatives, two flavonoids, one 2-pyrone derivative, and one undescribed naturally occurring compound, along with 30 known phenolic compounds, were isolated from Hypericum japonicum. In addition, hyperjaponols A and B were identified as racemates. The structures and...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2019.04.012
更新日期：2019-08-01 00:00:00
abstract：:Two new flavone glucosides, nevadensin 5-O-beta-D-glucoside and nevadensin 5-O-beta-D-glucosyl(1-->6)beta-D-glucoside, have been isolated from the aerial parts of Lysionotus pauciflorus. The structures have been determined by means of UV, mass spectral and one- and two-dimensional 1H and 13C NMR techniques. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(96)00066-0
更新日期：1996-07-01 00:00:00
abstract：:Three new ent-kaurene diterpenoids, oreskaurins A-C (1-3), together with ten known ent-kaurene diterpenoids, enmenin monoacetate (4), effusanin E (5), adenolin B (6), maoecrystal G (7), enmelol (8), trichokaurin (9), sodoponin (10), trichorabdal A (11), nodosin (12), enmein (13), and a flavonoid, vitexin (14), were is...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.02.022
更新日期：2004-04-01 00:00:00
abstract：:Eight pairs of enantiomeric lignans and neolignans including thirteen undescribed compounds, along with an undescribed meso compound, were isolated from the herbs of Solanum lyratum Thunb.(Solanaceae). Their structures and relative configurations were determined by extensive spectroscopic analyses of HRESIMS and nucle...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2020.112461
更新日期：2020-10-01 00:00:00
abstract：:OREB1 is a rice ABRE binding factor characterized by the presence of multiple highly-conserved phosphorylation domains (C1, C2, C3, and C4) and two kinase recognition motifs, RXXS/T and S/TXXE/D, within different functional domains. An in vitro kinase assay showed that OREB1 is phosphorylated not only by the SnRK2 kin...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2010.10.005
更新日期：2011-01-01 00:00:00
abstract：:An analytical strategy based on the sensitivity of electrospray tandem mass spectrometry following a simplified and reproducible sample preparation procedure was evaluated for the determination of Cd-induced phytochelatins (PC) and related peptides in four maize varieties. In addition to the three known families of PC...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00489-1
更新日期：2001-04-01 00:00:00
abstract：:Four new allose-containing triterpenoid saponosides, scabriosides A, B, C and D were isolated from the roots of Scabiosa rotata. Their structures were established as 3-O-beta-D-xylopyranosyl-28-O-[beta-D-allopyranosyl (1-->6)-beta-D-glucopyranosyl]-pomolic acid, 3-O-[alpha-L-rhamnopyranosyl (1-->2)-beta-D-xylopyranosy...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(97)00982-5
更新日期：1998-07-01 00:00:00
abstract：:Four p-terphenyl derivatives named curtisians E-H (1-4) were isolated from the methanolic extract of fruit bodies of the Basidiomycete Paxillus curtisii by a combination of Sephadex LH-20, silica gel column chromatography and preparative reversed phase HPLC. The relative stereochemistries of the curtisians were elucid...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(03)00263-2
更新日期：2003-09-01 00:00:00
abstract：:Two bitter stigmastane derivatives, vernoguinosterol (1) and vernoguinoside (2), have been isolated from the stem bark of Vernonia guineensis and their structures eludicated using spectroscopic methods. The new compounds exhibit trypanocidal activity. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(01)00448-4
更新日期：2002-02-01 00:00:00
abstract：:Chemical examination of the leaves of Styrax ferrugineus yielded 5-[3''-(beta-D-glucopyranosyloxy)propyl]-7-methoxy-2-(3',4'-dimethoxyphenyl) benzofuran, along with the known nor-lignans 5-(3''-hydroxypropyl)-7-methoxy-2-(3',4'-methylenedioxyphenyl) benzofuran, 5-(3''-hydroxypropyl)-7-methoxy-2-(3',4'-dimethoxyphenyl)...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00225-9
更新日期：2000-11-01 00:00:00
abstract：:Gynostemma pentaphyllum is a popular functional food, and it is also used as a traditional medicine in Asia. In this study, five previously undescribed triterpenes, gypensapogenin M, gypensapogenin N, gypensapogenin O, gypensapogenin P, and gypensapogenin Q, together with five known compounds were isolated from the hy...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2017.09.021
更新日期：2017-12-01 00:00:00
abstract：:Two forms of the pacA-encoded acid phosphatase (designated acid phosphatases I and II) secreted by the mold Aspergillus nidulans grown in low-Pi medium at 37 degrees, pH5.0, were purified to apparent homogeneity by PAGE. The M(r) of the purified enzyme forms were ca 115000 (60000) and 113000 (62000) respectively for f...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(98)00205-2
更新日期：1998-11-20 00:00:00
abstract：:Further investigation of the leaves and stems of Zygophyllum album afforded four triterpene glycosides which were characterized as 14-decarboxyquinovic acid-3 beta-O-beta-D-quinovopyranosyl (1-->4)-quinovopyranoside, quinovic acid 28-O-beta-D-glucopyranosyl (2-->1)beta-D-glucopyranosyl ester, quinovic acid 27-O-beta-D...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(93)85470-c
更新日期：1993-06-01 00:00:00
abstract：:The amorphane sesquiterpenes, deoxyarteannuin B and dihydro-deoxyarteannuin B, were isolated from Artemisia annua and their structures mainly determined by two-dimensional NMR spectoscopic analyses. The irregular monoterpene, trans-5-hydroxy-2-isopropenyl-5-methylhex-3-en-1-ol, was also characterized in the same way, ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(01)00358-2
更新日期：2001-12-01 00:00:00
abstract：:Glucosinolates are sulphur-containing glycosides found in brassicaceous plants that can be hydrolysed enzymatically by plant myrosinase or non-enzymatically to form primarily isothiocyanates and/or simple nitriles. From a human health perspective, isothiocyanates are quite important because they are major inducers of ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2009.07.035
更新日期：2009-07-01 00:00:00
abstract：:Six compounds from the aerial parts of the Argentinean plant Hymenoxys robusta (Rusby) Parker were isolated and their structures elucidated using extensive spectroscopic analyses. These compounds comprise two inositol derivatives and four 3,4-seco-pseudoguaianolides, including vermeerin. Bioactivity assays of these co...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2011.09.001
更新日期：2011-12-01 00:00:00
abstract：:The profiles of non-volatile oxylipins of pea (Pisum sativum) seedlings were examined by gas chromatography-mass spectrometry after invitro incubation with α-linolenic acid. The 13-lipoxygenase/hydroperoxide lyase (HPL) products were predominant in the leaves, while the roots possess both 13- and 9-HPL products. Allen...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2011.01.013
更新日期：2011-04-01 00:00:00
abstract：:Lotus japonicus roots, cultured in a modified B5 medium, produced and secreted germination stimulants that induced Striga hermonthica seed germination. The germination-inducing activity was detected both in the roots and the culture filtrate. Following bioassay-guided purification procedures, an active compound was is...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2007.06.011
更新日期：2008-01-01 00:00:00
abstract：:The essential oil of Salvia stenophylla contains (+)-3-carene as the principal monoterpene component. Using an enriched cDNA library prepared from mRNA isolated from S. stenophylla peltate glandular trichomes, and a homology-based cloning strategy, a full-length cDNA was isolated that encoded a preprotein of 69.7 kDa ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00674-x
更新日期：2003-04-01 00:00:00
abstract：:Valerophenone synthase (VPS) is a polyketide synthase that catalyzes the formation of the phloroglucinol derivatives in the synthesis of the bitter acids in hop (Humulus lupulus). The reaction uses isovaleryl-CoA or isobutyryl-CoA, but otherwise it is identical to that of the chalcone synthase in flavonoid biosynthesi...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(98)00346-x
更新日期：1998-12-01 00:00:00
abstract：:Three undescribed aporphine alkaloids laurodionine B (1), illigerine A (2), and N-formyl-laurolitsine (3) were isolated from the methanolic extracts of the Chinese medicinal plant, Illigera aromatica, together with three known analogues (4-6). The chemical structures of 1-6 were identified by spectroscopic methods inc...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2018.06.017
更新日期：2018-10-01 00:00:00
abstract：:Microbial transformation of psiadiarabin and its 6-desmethoxy analogue 5,3' dihydroxy-7,2',4'5'-tetramethoxyflavone by Cunninghamella elegans NRRL 1392 gave the 3'-glucoside conjugates of the two flavones. Structural elucidation of these two new metabolites was achieved using 1D and 2D NMR spectroscopy and CIMS. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
