Abstract:
:Phenolic composition of Ambrosia artemisiifolia L. pollen and sub-pollen particles (SPP) aqueous extracts was determined, using a novel extraction procedure. Total phenolic and flavonoid content was determined, as well as the antioxidative properties of the extract. Main components of water-soluble pollen phenolics are monoglycosides and malonyl-mono- and diglycosides of isorhamnetin, quercetin and kaempferol, while spermidine derivatives were identified as the dominant polyamides. SPP are similar in composition to pollen phenolics (predominant isorhamnetin and quercetin monoglycosides), but lacking small phenolic molecules (<450Da). Ethanol-based extraction protocol revealed one-third lower amount of total phenolics in SPP than in pollen. For the first time in any pollen species, SPP and pollen phenolic compositions were compared in detail, with an UHPLC/ESI-LTQ-Orbitrap-MS-MS approach, revealing the presence of spermidine derivatives in both SPP and pollen, not previously reported in Ambrosia species.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Mihajlovic L,Radosavljevic J,Burazer L,Smiljanic K,Cirkovic Velickovic Tdoi
10.1016/j.phytochem.2014.10.022subject
Has Abstractpub_date
2015-01-01 00:00:00pages
125-32eissn
0031-9422issn
1873-3700pii
S0031-9422(14)00426-9journal_volume
109pub_type
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