Abstract：
:Chloroacetamide herbicides inhibit very-long-chain fatty acid elongase, and it has been suggested that covalent binding to the active site cysteine of the condensing enzyme is responsible [Pest Manage Sci 56 (2000), 497], but direct evidence was not available. The proposal implied that other condensing enzymes might also be targets, and therefore we have investigated four purified recombinant type III plant polyketide synthases. Chalcone synthase (CHS) revealed a high sensitivity to the chloroacetamide metazachlor, with 50% inhibition after a 10 min pre-incubation with 1-2 molecules per enzyme subunit, and the inactivation was irreversible. Stilbene synthase (STS) inactivation required 20-fold higher amounts, and 4-coumaroyltriacetic acid synthase and pyrone synthase revealed no response at the highest metazachlor concentrations tested. A similar spectrum of differential responses was detected with other herbicides that also inhibit fatty acid elongase (metolachlor and cafenstrole). The data indicate that type III polyketide synthases are potential targets of these herbicides, but each combination has to be investigated individually. The interaction of metazachlor with CHS was investigated by mass spectrometric peptide mapping, after incubation of the enzymes with the herbicides followed by tryptic digestion. A characteristic mass shift and MS/MS sequencing of the respective peptide showed that metazachlor was covalently bound to the cysteine of the active site, and the same was found with STS. This is the first direct evidence that the active site cysteine in condensing enzymes is the primary common target of these herbicides.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Eckermann C,Matthes B,Nimtz M,Reiser V,Lederer B,Böger P,Schröder Jdoi
10.1016/s0031-9422(03)00516-8subject
Has Abstractpub_date
2003-11-01 00:00:00pages
1045-54issue
6eissn
0031-9422issn
1873-3700pii
S0031942203005168journal_volume
64pub_type
杂志文章相关文献
PHYTOCHEMISTRY文献大全abstract：:Compound-specific hydrogen and carbon isotopic compositions in n-alkanoic acids, phytol and sterols were determined for various plant classes (terrestrial C3-angiosperm; C3-gymnosperm; C4; crassulacean acid metabolism (CAM); and aquatic C3 plants) in order to investigate isotopic fractionations among various plant cla...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.03.036
更新日期：2004-05-01 00:00:00
abstract：:Recognition between mating partners, early sexual morphogenesis and development are regulated by a family of beta-carotene derived signal compounds, the trisporoids, in zygomycete fungi. Mating type-specific precursors are released from the hyphae and exert their physiological effects upon compatible mating partners. ...
journal_title：Phytochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.phytochem.2009.07.014
更新日期：2009-10-01 00:00:00
abstract：:The CH(2)Cl(2) extract of dried leaves of Helianthus annuus L. cv. Peredovick(R) has yielded, in addition to the known sesquiterpene lactones annuolide E and leptocarpin, and the sesquiterpenes heliannuols A, C, D, F, G, H, I, the new bisnorsesquiterpene, annuionone E, and the new sesquiterpenes heliannuol L, helibisa...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00370-9
更新日期：2002-11-01 00:00:00
abstract：:Two new long chain alkyl p-coumaric acid esters (2-3) along with eicosanyl trans-p-coumarate (1) were isolated from chloroform extract of the roots of Tanacetum longifolium. The structures of new compounds were assigned as 21'-hydroxyheneicosanyl-4-hydroxy-(cis and trans) p-coumarate (2a, 2b) and 27'-hydroxy heptacosa...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2003.08.023
更新日期：2003-10-01 00:00:00
abstract：:Strychnos nux-vomica L. (Loganiaceae) is famous for its monomeric alkaloid content, such as strychnine, a convulsant poison. The stem bark of the tree is traditionally used to treat intermittent fever in South East Asia. In various studies, it appeared that dimeric indolo-monoterpenic alkaloids possess a promising act...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2012.11.002
更新日期：2013-03-01 00:00:00
abstract：:Amaranthus hypochondriacus L. infected with Ageratum enation virus (AEV) was investigated for identifying alteration in the anatomical structures, sap translocation and metabolomic variations using light microscopy, magnetic resonance imaging, NMR spectroscopy and GC-MS, respectively. Combination of GC-MS and NMR spec...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2012.05.007
更新日期：2012-08-01 00:00:00
abstract：:Phytoalexins are inducible chemical defenses produced by plants in response to diverse forms of stress, including microbial attack. Our search for phytoalexins from cruciferous plants resistant to economically important fungal diseases led us to examine stinkweed or pennycress (Thlaspi arvense), a potential source of ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(03)00441-2
更新日期：2003-11-01 00:00:00
abstract：:An analytical strategy based on the sensitivity of electrospray tandem mass spectrometry following a simplified and reproducible sample preparation procedure was evaluated for the determination of Cd-induced phytochelatins (PC) and related peptides in four maize varieties. In addition to the three known families of PC...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00489-1
更新日期：2001-04-01 00:00:00
abstract：:Six phragmalin limonoids, named tabulalin and tabulalides A-E, were isolated from the root bark of Chukrasia tabularis (Meliaceae). Their structures were determined by spectroscopic methods, and their antifeedant properties evaluated. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.08.010
更新日期：2004-10-01 00:00:00
abstract：:The essential oil from the leaves of Callicarpa japonica was analyzed by GC-MS, and 84 compounds were identified. The main constituents of the essential oil were spathulenol (18.1%), germacrene B (13.0%), bicyclogermacrene (11.0%), globulol (3.3%), viridiflorol (2.6%), alpha-guaiene (2.3%), and gamma-elemene (2.0%). T...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00207-8
更新日期：2002-09-01 00:00:00
abstract：:The inhibition of wheat Ca(2+)-dependent protein kinase (CDPK) by substituted acridines and substituted 5-, 6-, 7- and 8-azaacridines (5-AA, 6-AA, 7-AA and 8-AA) was examined. Of a total of 71 substituted acridines and azaacridines examined, only 20 have IC50 values for wheat CDPK of less than 200 microM. Of these, ef...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)89629-6
更新日期：1994-07-01 00:00:00
abstract：:Eleven indole alkaloids, comprising four corynanthean, two eburnane, one aspidofractinine, one secoleuconoxine, one andranginine, and two pauciflorine type alkaloids were isolated from the stem-bark and leaf extracts of Kopsia pauciflora. Their structures were determined using NMR and MS analyses. The catharinensine t...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2014.09.014
更新日期：2014-12-01 00:00:00
abstract：:The study of phosphoproteome on a global scale represents one of the challenges in the post-genomic era. Here, we propose an integrated procedure starting from the crude protein extract, that consists of sequential purification steps, and ending up in the identification of phosphorylation sites. This involves (i) an e...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2006.07.010
更新日期：2006-10-01 00:00:00
abstract：:A novel minor triterpenoid saponin mimusin (3-O-[beta-D-glucopyranosyl- (1-->6)-beta-D-glucopyranosyl]-2 beta,3 beta,6 beta,23-tetrahydroxyolean- 12-en-28-oic acid 28-O-alpha-L-rhamnopyranosyl-(1-->3)-beta-D-xylopyranosyl-(1-->4)-alpha- L- rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranoside was isolated from the seeds o...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(96)00659-0
更新日期：1997-03-01 00:00:00
abstract：:Nine new bidesmosidic 3-O-glucuronide oleanane triterpenoid saponins were isolated from the stem bark of Symplocos glomerata King along with two known saponins, salsoloside C and copteroside E, and two major lignans, (-)-pinoresinol and (-)-pinoresinol-4'-O-beta-D-glucopyranoside. The structures of the new saponins we...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.01.012
更新日期：2004-03-01 00:00:00
abstract：:Pinoresinol/lariciresinol reductase (PLR), an NADPH-dependent reductase that catalyzes the sequential reduction of pinoresinol into secoisolariciresinol via Lariciresinol, can lead to the structural and stereochemical diversity of lignans. The relationship between substrate-selective reaction of PLR and sequence homol...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2019.02.003
更新日期：2019-06-01 00:00:00
abstract：:Although it is well known that solar ultraviolet B (UV-B) radiation enhances plant defenses, there is less knowledge about traits that define insect resistance in field-grown soybean. Here we study the effects of solar UV-B radiation on: a) the induction of phenolic compounds and trypsin proteinase inhibitors (TPI) in...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2017.05.006
更新日期：2017-09-01 00:00:00
abstract：:In this study we were mirroring suggested in vivo phenomena of lignin-hemicellulose complex formation in vitro, by cross-linking Norway spruce (Picea abies) galactoglucomannans, xylans and lignin moieties to high molecular weight complexes by laccase treatment. We were able to observe the oxidation and cross-linking o...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2014.10.027
更新日期：2015-03-01 00:00:00
abstract：:Four undescribed pyrone derivatives, pestalopyrones A-D, containing unusual tricyclic 5/6/6 polycyclic skeletons, were isolated from the ethyl acetate extract of the endophytic fungus Pestalotiopsis neglecta S3 derived from the fresh stems of Rubia podantha Diels (Rubiaceae). Their planar structures were elucidated ma...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2020.112505
更新日期：2020-11-01 00:00:00
abstract：:A lateral bud growth inhibitor was isolated from etiolated pea seedlings and identified as indole-3-aldehyde. The indole-3-aldehyde content was significantly higher in the diffusates from explants with apical bud and indole-3-acetic acid treated decapitated explants, in which apical dominance is maintained, than in th...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00400-4
更新日期：2002-12-01 00:00:00
abstract：:The amorphane sesquiterpenes, deoxyarteannuin B and dihydro-deoxyarteannuin B, were isolated from Artemisia annua and their structures mainly determined by two-dimensional NMR spectoscopic analyses. The irregular monoterpene, trans-5-hydroxy-2-isopropenyl-5-methylhex-3-en-1-ol, was also characterized in the same way, ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(01)00358-2
更新日期：2001-12-01 00:00:00
abstract：:Santolina insularis (Genn ex Fiori) Arrig. is a medicinal plant whose essential oil shows antiviral and antibacterial activities and potent and selective cytotoxic activity against the human colon carcinoma cell line. The occurrence of several chemotypes makes the taxonomic identification of S. insularis hard to achie...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2010.03.012
更新日期：2010-06-01 00:00:00
abstract：:Glucosinolates are plant secondary metabolites that are part of a plant defence system against pathogens and pests, the myrosinase-glucosinolate system, in which glucosinolates get activated by enzymic degradation through thioglucoside glucohydrolases called myrosinases. Epithiospecifier protein (ESP) and nitrile-spec...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2012.08.004
更新日期：2012-12-01 00:00:00
abstract：:The incubation of 15α-hydroxy-ent-kaur-9(11),16-dien-19-oic acid (15α-hydroxy-grandiflorenic acid) with the fungus Fusarium fujikuroi gave as main metabolite its 3β,6β-dihydroxy derivative, which by an oxidative decarboxylation afforded a 19-nor compound with a 4,18-double bond. Other substances obtained were a 3α-hyd...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2013.01.006
更新日期：2013-05-01 00:00:00
abstract：:From the water-soluble part of an extract of Aragoa cundinamarcensis were isolated seven iridoid glucosides, namely aucubin, catalpol, rehmannioside D, globularin, gardoside methyl ester, epiloganin and mussaenoside. The main glycoside isolated, however, was a new caffeoyl phenylethanoid triglycoside, named aragoside,...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(03)00157-2
更新日期：2003-09-01 00:00:00
abstract：:The interest in the potential health effects of dietary phytoestrogens has increased with the findings that hormone replacement therapy is not as safe or effective as previously thought. This review summarizes the dietary sources of the phytoestrogens; isoflavonoids, stilbenes, coumestans and lignans. It also examines...
journal_title：Phytochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.phytochem.2004.03.005
更新日期：2004-04-01 00:00:00
abstract：:Nine undescribed compounds, including five xanthone derivatives, two flavonoids, one 2-pyrone derivative, and one undescribed naturally occurring compound, along with 30 known phenolic compounds, were isolated from Hypericum japonicum. In addition, hyperjaponols A and B were identified as racemates. The structures and...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2019.04.012
更新日期：2019-08-01 00:00:00
abstract：:Sixteen previously undescribed lanostane-type triterpenoids (1-16), together with fourteen known compounds, were isolated from cultivated fruiting bodies of the basidiomycete Ganoderma casuarinicola, a recently described species. The structures were elucidated on the basis of NMR spectroscopic and mass spectrometry da...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2019.112225
更新日期：2020-02-01 00:00:00
abstract：:Toddaliopsins A-D, four novel 1,2,3-trioxygenated acridone alkaloids, have been isolated from the leaves of Toddaliopsis bremekampii. Toddaliopsins B-D are the first reported acridone alkaloids with substituted N-methyl groups, in the light of which the chemotaxonomic relationship of Toddaliopsis and Vepris is discuss...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2005.04.036
更新日期：2005-07-01 00:00:00
abstract：:Seventeen undescribed heterodimers of meroterpene phenol, psocorylins A-Q, were isolated from the fruits of Psoralea corylifolia. Their another monomeric unit derived from flavonone, chalcone, coumarin and isoflavone, respectively. Psocorylins A-E were rare natural spiroketals with the skeleton of 1,4,8-trioxaspiro[4....
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2020.112394
更新日期：2020-08-01 00:00:00
