Abstract：
:Santolina insularis (Genn ex Fiori) Arrig. is a medicinal plant whose essential oil shows antiviral and antibacterial activities and potent and selective cytotoxic activity against the human colon carcinoma cell line. The occurrence of several chemotypes makes the taxonomic identification of S. insularis hard to achieve. GC-MS essential oil analyses of four chemotypes (SI1, SI2, SI3 and SI4) revealed the presence of different percentages of santolina triene, beta-pinene, myrcene, beta-phellandrene, artemisia ketone and cis-chrysanthemol, allowing a chemical discrimination. Single fragments of the 5S-rRNA-NTS region of approximately 150, 170, 260 and 280bp were produced by SI1, SI2, SI3 and SI4, respectively, and the sequence alignment of the 5S-rRNA spacer region flanked by the 3'-and 5'-ends of the coding region confirmed a consistent difference between chemotypes. Furthermore, a PCR-RFLP method was applied. From the identified sequences, an EcoRV site could be found in chemotypes SI1, SI2 and SI3 in the 5S-rRNA spacer regions at 81 bp position; however, this site was absent in the chemotype SI4. This study, by showing remarkable chemical variation in the terpenoid profile and consistent genomic difference in the 5S-rRNA spacer regions, identified four chemotypes of S. insularis which could be grouped into two ecotypes, based on chemical and genomic analyses. The identification of specific gene sequences of the 5S-rRNA-NTS region and of a EcoRV site identified in this work can be used for a rapid and precise identification of the plant chemo-/ecotypes, complementing the essential oil chemical analysis.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Gnavi G,Bertea CM,Usai M,Maffei MEdoi
10.1016/j.phytochem.2010.03.012subject
Has Abstractpub_date
2010-06-01 00:00:00pages
930-6issue
8-9eissn
0031-9422issn
1873-3700pii
S0031-9422(10)00090-7journal_volume
71pub_type
杂志文章相关文献
PHYTOCHEMISTRY文献大全abstract：:Profiles of terpenoid glycoside metabolites in glandular trichomes of Solanum habrochaites LA1777 leaves were generated using ultrahigh performance liquid chromatography/time-of-flight mass spectrometry with multiplexing of non-selective collision-induced dissociation (CID). Profiling data suggested a diverse group of...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2013.11.011
更新日期：2014-02-01 00:00:00
abstract：:Crotaoprostrin, a chalcone not yet known as a plant constituent, was isolated from the aerial parts of the Indian medicinal plant Crotalaria prostrata. The structures of the chalcone polyarvin and the partially hydrogenated naturally occurring derivatives crotaramin, crotaramosmin, and crotin were confirmed by chemica...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00475-2
更新日期：2002-12-01 00:00:00
abstract：:Three-day-old soybean (Glycine max) seedlings were exposed to 0.4 M sorbitol solution for 4 h to induce amidinotransferase activity, with the corresponding enzyme being purified to homogeneity by chromatographic separation on DEAE-Sephacel, Sephacryl S-300 and L-arginine Sepharose 4B. The purified enzyme used L-argini...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00401-6
更新日期：2002-12-01 00:00:00
abstract：:Lotus japonicus roots, cultured in a modified B5 medium, produced and secreted germination stimulants that induced Striga hermonthica seed germination. The germination-inducing activity was detected both in the roots and the culture filtrate. Following bioassay-guided purification procedures, an active compound was is...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2007.06.011
更新日期：2008-01-01 00:00:00
abstract：:3β-Hydroxy-5β-cholestan-6-one was identified in the EtOAc extract of Ajuga hairy roots by micro-analysis using LC-MS/MS in the multiple reaction mode (MRM). Furthermore, administration of (2,2,4,4,7,7-(2)H6)- and (2,2,4,4,6,7,7-(2)H7)-cholesterols to the hairy roots followed by LC-MS/MS analysis of the EtOAc extract o...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2014.12.019
更新日期：2015-03-01 00:00:00
abstract：:From the pro-inflammatory active extract of Euphorbia peplus, a new diterpene polyester (1) based on the jatrophane skeleton was isolated together with the known compounds 2-5. The irritant activities of some jatrophane diterpenes (2, 3 and 6-9) were also investigated: only compound 2 was found to exert a weak pro-inf...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(99)00088-6
更新日期：1999-07-01 00:00:00
abstract：:The seeds of 36 pigeonpea [Cajanus cajan (L) Millsp.] cultivars, resistant and susceptible to pests and pathogens and 17 of its wild relatives were analysed for inhibitors of trypsin, chymotrypsin, and insect gut proteinases to identify potential inhibitors of insect (Helicoverpa armigera) gut enzymes. Proteinase inhi...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(03)00375-3
更新日期：2003-10-01 00:00:00
abstract：:From dengue to herpes and influenza to AIDS, the phytoestrogens that are present in many fruits and vegetables have been shown to exert anti-viral properties. Here we review the various different anti-viral mechanisms employed by phytoestrogens. ...
journal_title：Phytochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.phytochem.2006.11.018
更新日期：2007-02-01 00:00:00
abstract：:A high purity of β-D-glucan (80.8%) from hull-less barley (Hordeum vulgare L. var. nudum Hook. f.) (HBBG) was isolated by alkali extraction and multi-precipitation with ethanol. The molecular weight (Mw) of HBBG was determined as 571.4 kDa with a broad distribution (Mw/Mn = 1.6) by using HPSEC. According to methylatio...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2018.08.004
更新日期：2018-11-01 00:00:00
abstract：:The CH(2)Cl(2) extract of dried leaves of Helianthus annuus L. cv. Peredovick(R) has yielded, in addition to the known sesquiterpene lactones annuolide E and leptocarpin, and the sesquiterpenes heliannuols A, C, D, F, G, H, I, the new bisnorsesquiterpene, annuionone E, and the new sesquiterpenes heliannuol L, helibisa...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00370-9
更新日期：2002-11-01 00:00:00
abstract：:Triterpenoid saponins, impatienosides A-G, together with 12 known saponins, were isolated from the whole plants of Impatiens siculifer. Their structures were established on the basis of extensive 1D and 2D NMR and MS analyses coupled with chemical degradation. Cytotoxic activities of the isolated saponins were evaluat...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2009.03.022
更新日期：2009-04-01 00:00:00
abstract：:The patterns of secondary metabolites in leaves of yeast invertase-transgenic tobacco plants (Nicotiana tabacum L. cv. Samsun NN) were analyzed. Plants expressing cytosolic yeast-derived invertase (cytInv) or apoplastic (cell wall associated) yeast invertase (cwInv) showed a characteristic phytochemical phenotype comp...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00422-2
更新日期：2001-03-01 00:00:00
abstract：:2,5-Dimethyl-4-hydroxy-3[2H]-furanone 6'-O-malonyl-beta-D-glucopyranoside was isolated from a glycosidic extract of strawberry fruit (Fragaria x ananassa, cv. Senga Sengana) by means of countercurrent chromatography and reverse-phase HPLC. Identification was achieved by comparison of chromatographic and 1H, 13C and 2D...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(96)00216-6
更新日期：1996-09-01 00:00:00
abstract：:Leishmanicidal activity of 6alpha, 7alpha, 15beta, 16beta, 24-pentacetoxy-22alpha-carbometoxy-21beta,22beta-epoxy-18beta-hydroxy-27,30-bisnor-3,4-secofriedela-1,20 (29)-dien-3,4 R-olide (LLD-3 (1)) isolated from Lophanthera lactescens Ducke, a member of the Malpighiaceae, was demonstrated against intramacrophage amast...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2009.03.009
更新日期：2009-03-01 00:00:00
abstract：:Four new oleanane-type saponins 3-O-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucuronopyranosyl-22-O-β,β-dimethylacryloylA1-barrigenol (1), 3-O-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucuronopyranosyl-22-O-angeloylA1-barrigenol (2), 3-O-β-D-glucopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1 → 6)]-β-D-glucopyranosyl-21,22,28-O-triacetyl-(...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2014.11.017
更新日期：2015-02-01 00:00:00
abstract：:The corolla of Brassica rapa has an UV-absorbing zone in its center, known as the nectar guide for attracting pollinating insects. The pigment which plays the role of the nectar guide was isolated from the petals and identified to be isorhamnetin 3,7-O-di-beta-D-glucopyranoside on the basis of MS and NMR spectroscopic...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00237-6
更新日期：2002-10-01 00:00:00
abstract：:Five novel polyphenols have been isolated from the air-dried herb of Cistus salvifolius; they were characterized as epigallocatechin-3-O-(4-hydroxybenzoate), epigallocatechin-(4 beta-->8)-epigallocatechin, epigallocatechin-3-O- gallate-(4 beta-->8)-epigallocatechin, epigallocatechin-(4 beta-->6)-epigallocatechin-3-O-g...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(94)85094-1
更新日期：1994-09-01 00:00:00
abstract：:Oleanolic acid (3β-hydroxyolean-12-en-28-oic acid) is a pentacyclic triterpenoid compound with a widespread occurrence throughout the plant kingdom. In nature, the compound exists either as a free acid or as an aglycone precursor for triterpenoid saponins, in which it can be linked to one or more sugar chains. Oleanol...
journal_title：Phytochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.phytochem.2011.12.022
更新日期：2012-05-01 00:00:00
abstract：:Four cucurbitacins, bacobitacin A-D (1-4) as well as, a known cytotoxic, cucurbitacin E (5) together with three known phenylethanoid glycosides, monnieraside I, III and plantioside B were isolated form the aerial part of Bacopa monnieri. Their structures were elucidated on the basis of extensive spectroscopic investig...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2007.03.013
更新日期：2007-05-01 00:00:00
abstract：:Lathyrus sativus (grass pea) is a high-yielding, drought-resistant legume consumed as a food in Northern India and neighboring countries as well as in Ethiopia. Its development into an important food legume, however, has been hindered by the presence of the neurotoxin - beta-N-oxalyl-L-alpha,beta-diaminopropionic acid...
journal_title：Phytochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.phytochem.2005.10.022
更新日期：2006-01-01 00:00:00
abstract：:The leaves of Aglaia edulis afforded a new bisamide, aglaiduline, and two new sulfur-containing bisamides, aglaithioduline and aglaidithioduline. Their structures were established from spectroscopic studies. The sulfur-containing amides exhibited slight antiviral activity against herpes simplex virus types 1 and 2. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(99)00378-7
更新日期：1999-11-01 00:00:00
abstract：:Four new allose-containing triterpenoid saponosides, scabriosides A, B, C and D were isolated from the roots of Scabiosa rotata. Their structures were established as 3-O-beta-D-xylopyranosyl-28-O-[beta-D-allopyranosyl (1-->6)-beta-D-glucopyranosyl]-pomolic acid, 3-O-[alpha-L-rhamnopyranosyl (1-->2)-beta-D-xylopyranosy...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(97)00982-5
更新日期：1998-07-01 00:00:00
abstract：:Glucosinolates are plant secondary metabolites that are part of a plant defence system against pathogens and pests, the myrosinase-glucosinolate system, in which glucosinolates get activated by enzymic degradation through thioglucoside glucohydrolases called myrosinases. Epithiospecifier protein (ESP) and nitrile-spec...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2012.08.004
更新日期：2012-12-01 00:00:00
abstract：:A phytochemical investigation of the leaf exudate of Aloe marlothii has resulted in the isolation of a new chromone (7-O-methylaloeresin A) and a new anthrone (5-hydroxyaloin A 6'-O-acetate). Furthermore 7-O-methylaloesin was isolated as a natural product for the first time from the leaf exudate of Aloe rupestris. The...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00328-9
更新日期：2000-12-01 00:00:00
abstract：:Cyclization of methyl ent-8 alpha-hydroxylabd-13(16),14-dien-18-oate with m-chloroperbenzoic acid gave methyl (13S)-ent-16-hydroxy-8 alpha,13-epoxylabd-14-en-18-oate and its epimer at C-13. Biotransformation of the former (which exhibits antileishmania activity) with Rhizopus nigricans cultures produced the methyl (13...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(94)00549-9
更新日期：1995-01-01 00:00:00
abstract：:The CH(2)Cl(2) extract of aerial parts of Eupatorium perfoliatum L. exhibits antiprotozoal activity under in vitro conditions, especially against Plasmodium falciparum (IC(50)=2.7μg/ml). The search for active compounds yielded seven sesquiterpene lactones: Four structurally similar guaianolides, one dimeric guaianolid...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2011.01.025
更新日期：2011-05-01 00:00:00
abstract：:Human kidney-type glutaminase (KGA) is an important target that is often over expressed in many cancer cells but very few effective inhibitors of this enzyme have yet reached clinical trials. Caudatan A and caudatan B, two undescribed tetracyclic flavans with an unusual ether bond between the C-4 and C-2' were isolate...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2018.04.013
更新日期：2018-08-01 00:00:00
abstract：:Phytochemical investigation of the flowers of Inula japonica led to isolation of nine sesquiterpenoids, inujaponins A-I, as well as eighteen known ones. These sesquiterpenoids belong to six skeletal-types, including eudesmane, 1,10-seco-eudesmane, germacrane, guaiane, 4,5-seco-guaiane, and pseudoguaiane sesquiterpenoi...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2016.07.008
更新日期：2016-09-01 00:00:00
abstract：:In an attempt to reveal the relationships between alkaloid biosynthesis and phylogeny, we investigated by GC-MS the alkaloid patterns of 22 species and 3 hybrids (from 45 locations) from seven main sections of the genus Narcissus (Amaryllidaceae). The results indicate that the first alkaloids to evolve in the genus Na...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2013.11.002
更新日期：2014-03-01 00:00:00
abstract：:In plant terpene biosynthesis, oxidation of the hydrocarbon backbone produced by terpene synthases is typically carried out by cytochrome P450 oxygenases (CYPs). The modifications introduced by CYPs include hydroxylations, sequential oxidations at one position and ring rearrangements and closures. These reactions sign...
journal_title：Phytochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.phytochem.2018.12.003
更新日期：2019-05-01 00:00:00
