Abstract：
:3β-Hydroxy-5β-cholestan-6-one was identified in the EtOAc extract of Ajuga hairy roots by micro-analysis using LC-MS/MS in the multiple reaction mode (MRM). Furthermore, administration of (2,2,4,4,7,7-(2)H6)- and (2,2,4,4,6,7,7-(2)H7)-cholesterols to the hairy roots followed by LC-MS/MS analysis of the EtOAc extract of the hairy roots indicated that cholesterol was converted to the 5β-ketone with hydrogen migration from the C-6 to the C-5 position. These findings, in conjunction with the previous observation that the ketone was efficiently converted to 20-hydroxyecdysone, strongly suggest that the 5β-ketone is an intermediate immediately formed after cholesterol during 20-hydroxyecdysone biosynthesis in Ajuga sp. In addition, the mechanism of the 5β-ketone formation from cholesterol is discussed.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Fujimoto Y,Maeda I,Ohyama K,Hikiba J,Kataoka Hdoi
10.1016/j.phytochem.2014.12.019subject
Has Abstractpub_date
2015-03-01 00:00:00pages
59-64eissn
0031-9422issn
1873-3700pii
S0031-9422(14)00551-2journal_volume
111pub_type
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