Abstract：
:Amaranthus hypochondriacus L. infected with Ageratum enation virus (AEV) was investigated for identifying alteration in the anatomical structures, sap translocation and metabolomic variations using light microscopy, magnetic resonance imaging, NMR spectroscopy and GC-MS, respectively. Combination of GC-MS and NMR spectroscopy identified 68 polar and non-polar metabolites that were present in different levels in healthy and virus-infected A. hypochondriacus. Contrast of T₁ and T₂ weighted MR images showed significant differences in the spatial distribution of water, lipids and macromolecules indicating alterations in the cortical region and disruption of vascular bundles in virus-infected stem tissues. MRI observations are supported by light microscopic examination. Microscopic examination of AEV infected stem revealed severe hyperplasia with a considerable reduction in size of stem cells. The NMR spectroscopy and GC-MS analysis indicated that viral infection significantly affected the plant primary and secondary metabolism resulting in decreased glucose and sucrose content and increase in the concentration of β-sitosterol and stigmasterol. Higher accumulation of TCA cycle intermediates such as citric acid and malic acid in AEV infected plants indicated enhanced rate of respiratory metabolism. The viral stress significantly increases the concentration of erythritol and myo-inositol as compared to healthy ones. Lower concentration of glucose and sucrose in viral-infected stem tissues suggests decreased translocation of photosynthates in the plants. The results demonstrated potential of MRI, NMR spectroscopy and GC-MS for studying anatomical and metabolic variations in virus-infected plants.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Srivastava S,Bisht H,Sidhu OP,Srivastava A,Singh PC,Pandey RM,Raj SK,Roy R,Nautiyal CSdoi
10.1016/j.phytochem.2012.05.007subject
Has Abstractpub_date
2012-08-01 00:00:00pages
8-16eissn
0031-9422issn
1873-3700pii
S0031-9422(12)00207-5journal_volume
80pub_type
杂志文章相关文献
PHYTOCHEMISTRY文献大全abstract：:A benzoquinone, named alopecuquinone, was isolated from the ethanol extract of the inflorescences of Cyperus alopecuroides. Its structure was primarily elucidated by spectroscopic analysis including 1H, 13C NMR, APT, HMQC, 1H-1H COSY and CIMS. The known flavonoids, vicenin 2, orientin, diosmetin, quercetin 3,3'-dimeth...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00060-2
更新日期：2002-06-01 00:00:00
abstract：:The tetrahydroanthraquinones (1S,3S)-austrocortilutein (4) and (1S,3S)-austrocortirubin (7) are octaketides formed in the fruit bodies of the fungus Dermocybe splendida via the gentiobioside 2 of the dihydroanthracenone (S)-torosachrysone (1). ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00181-3
更新日期：2000-09-01 00:00:00
abstract：:The combination of the hyphenated techniques LC-MS and LC-SPE-NMR constitutes a powerful platform for the rapid isolation and identification of minor components from natural sources. Electronic circular dichroism (ECD) is a useful tool to determine the absolute configuration of small quantities of chiral molecules. In...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2012.04.004
更新日期：2012-07-01 00:00:00
abstract：:Silene species are known for their use in traditional medicine in treating several diseases. To the authors' knowledge there is no report on the chemical composition of S. odontopetala. Therefore, the phytochemical investigation of the methanol extract of S. odontopetala was carried out, leading to the isolation of si...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2020.112404
更新日期：2020-08-01 00:00:00
abstract：:The structure of four diterpenoid glucosides, designated as salvigresides A-D (1-4), isolated from the aerial parts of Salvia greggii, have been confirmed by spectroscopic and chemical investigation. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.08.012
更新日期：2004-09-01 00:00:00
abstract：:Eight lignan derivatives trilobatin D-K, as well as jamesopyrone were isolated from the liverwort Bazzania trilobata. Their structures have been elucidated based on extensive NMR spectral evidence. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00626-x
更新日期：2003-03-01 00:00:00
abstract：:Blueberry consumption is associated with health benefits contributing to a reduced risk for cardiovascular disease, diabetes and cancer. The aim of this study was to determine the anthocyanin profile of blueberry extracts and to evaluate their effects on B16-F10 metastatic melanoma murine cells. Seven blueberry cultiv...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2013.06.018
更新日期：2013-11-01 00:00:00
abstract：:The stimulation of the plant response to pathogen attack by the application of resistance inducers, called elicitors, could represent an environmentally and commercially viable alternative or complement to existing pathogen control methods. In this work, the elicitor chitosan was sprayed on grape (Vitus vinifera L.) b...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2018.08.011
更新日期：2018-12-01 00:00:00
abstract：:Pumpkin (Cucurbita pepo) seeds are used locally in Eritrea to treat tapeworm. Seeds were found to be rich in oil (approximately 35%), protein (38%), alpha-tocoferols (3 mg/100 g) and carbohydrate content (approximately 37%). The physico-chemical properties and fatty acid composition of the seed oil were examined. The ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(99)00610-x
更新日期：2000-05-01 00:00:00
abstract：:Aerial parts of Aconitum variegatum L. from the Pyrenees furnished four norditerpene alkaloids, 16 beta-hydroxycardiopetaline, 8-ethoxysachaconitine, 14-acetylgenicunine B, N-deethyl-N-19-didehydrosachaconitine, five diterpene alkaloids 15-veratroyldictizine, 15-veratroyl-17-acetyldictizine, 15-veratroyl-17-acetyl-19-...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2005.01.019
更新日期：2005-04-01 00:00:00
abstract：:Glucosinolates are secondary plant compounds typically found in members of the Brassicaceae and a few other plant families. Usually each plant species contains a specific subset of the ∼ 130 different glucosinolates identified to date. However, intraspecific variation in glucosinolate profiles is commonly found. Sinal...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2014.11.016
更新日期：2015-02-01 00:00:00
abstract：:Five novel polyphenols have been isolated from the air-dried herb of Cistus salvifolius; they were characterized as epigallocatechin-3-O-(4-hydroxybenzoate), epigallocatechin-(4 beta-->8)-epigallocatechin, epigallocatechin-3-O- gallate-(4 beta-->8)-epigallocatechin, epigallocatechin-(4 beta-->6)-epigallocatechin-3-O-g...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(94)85094-1
更新日期：1994-09-01 00:00:00
abstract：:The paper describes the amino acid sequence of a 26 kDa basic subunit of 13S globulin of common buckwheat (Fagopyrum esculentum Moench). The protein has 93 and 75% sequence homology with 11S globulin of Coffea arabica and beta subunit of 11S globulin of Cucurbita pepo respectively. The subunit has the "globally conser...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00755-0
更新日期：2003-05-01 00:00:00
abstract：:Six new and three synthetically known glycosides have been isolated from the leaves of Moringa oleifera, employing a bioassay-directed isolation method on the ethanolic extract. Most of these compounds, bearing thiocarbamate, carbamate or nitrile groups, are fully acetylated glycosides, which are very rare in nature. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(94)00729-d
更新日期：1995-03-01 00:00:00
abstract：:The chemical investigation of leaves of Bridelia glauca f. balansae afforded six megastigmane glucosides, named bridelionosides A-F, along with seven known megastigmane glucosides. Their structures were determined by a combination of spectroscopic analyses and by application of the modified Mosher's method. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2006.09.007
更新日期：2006-11-01 00:00:00
abstract：:A structure-activity relationships study was conducted assaying 15 natural analogues and derivatives belonging to two groups of organic compounds, nonenolides and cytochalasins, for their toxicity against the composite perennial weeds Cirsium arvense and Sonchus arvensis occurring through the temperate region of world...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2007.11.003
更新日期：2008-02-01 00:00:00
abstract：:Two pimarane diterpenes structurally related to sphaeropsidins were isolated from the liquid culture of Sphaeropsis sapinea f. sp. cupressi, a plant pathogenic fungus causing a form of canker disease of Italian cypress (Cupressus sempervirens L.). The two metabolites, characterised by spectroscopic methods, were named...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00015-8
更新日期：2002-04-01 00:00:00
abstract：:Three-day-old soybean (Glycine max) seedlings were exposed to 0.4 M sorbitol solution for 4 h to induce amidinotransferase activity, with the corresponding enzyme being purified to homogeneity by chromatographic separation on DEAE-Sephacel, Sephacryl S-300 and L-arginine Sepharose 4B. The purified enzyme used L-argini...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00401-6
更新日期：2002-12-01 00:00:00
abstract：:Bioassay guided fractionation of the lipophilic extract of the marine cyanobacterium Phormidium ectocarpi yielded a new natural product hierridin B and the previously described compound 2,4-dimethoxy-6-heptadecyl-phenol. Both structures were secured by extensive spectroscopic analysis (1D and 2D NMR, MS, GC-MS, IR). T...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(98)00440-3
更新日期：1998-12-01 00:00:00
abstract：:Telomeres are nucleoprotein structures ensuring the stability of eukaryotic chromosome ends. Two protein families, TRFL (TFL-Like) and SMH (Single-Myb-Histone), containing a specific telobox motif in their Myb domain, have been identified as potential candidates involved in a functional nucleoprotein structure analogo...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2008.04.001
更新日期：2008-06-01 00:00:00
abstract：:Smoke derived karrikinolide and trimethylbutenolide exerted neuroprotective effects against monoamine oxidase and acetylcholinesterase. Synthesis of potent analogs was achieved. Sulphur substitution in the bicyclic ring structure of KAR1 displayed the most encouraging activity returning IC50 values of 13.75 ± 0.001 μM...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2019.03.014
更新日期：2019-07-01 00:00:00
abstract：:Nine undescribed compounds, including five xanthone derivatives, two flavonoids, one 2-pyrone derivative, and one undescribed naturally occurring compound, along with 30 known phenolic compounds, were isolated from Hypericum japonicum. In addition, hyperjaponols A and B were identified as racemates. The structures and...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2019.04.012
更新日期：2019-08-01 00:00:00
abstract：:The structure of cephalaria saponin B, isolated from Cephalaria transsylvanica was elucidated as 3-O-([beta-D-xylopyranosyl(1-->4)-alpha-L-rhamnopyranosyl(1-->2)-beta-D- xylopyranosyl]-[28-O-beta-D-glucopyranosyl])-3beta,23-dihydroxy-12(13)-oleanene-28-oic acid. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(94)00789-v
更新日期：1995-04-01 00:00:00
abstract：:Due to their pronounced cytotoxic activity, a number of aryltetralin lignans (ATLs), such as podophyllotoxin (PTOX), are used as antitumor compounds. The production of such molecules from entire plants or plant cell-tissue-organ cultures is thus of interest to the pharmaceutical industry. Hairy root cultures constitut...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2015.01.001
更新日期：2015-07-01 00:00:00
abstract：:Evidence is presented for the presence of xylogalacturonan (XGA) in Arabidopsis thaliana. This evidence was obtained by extraction of pectin from the seeds, root, stem, young leaves and mature leaves of A. thaliana, followed by treatment of these pectin extracts with xylogalacturonan hydrolase (XGH). Upon enzymatic tr...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2007.01.016
更新日期：2007-04-01 00:00:00
abstract：:Destruxins, secondary metabolites first reported in 1961, are cyclic hexadepsipeptides composed of an alpha-hydroxy acid and five amino acid residues. The name "destruxin" is derived from "destructor" from the species Oospora destructor, the entomopathogenic fungus from which these metabolites were first isolated. Ind...
journal_title：Phytochemistry
pub_type： 杂志文章,评审
doi：10.1016/s0031-9422(02)00016-x
更新日期：2002-03-01 00:00:00
abstract：:The essential oil of the east African sandalwood Osyris tenuifolia was investigated by chromatographic and spectroscopic methods. Beside several already known sesquiterpenes four new compounds could be isolated by preparative gas chromatography and their structures investigated by mass spectroscopy and NMR techniques....
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.05.007
更新日期：2004-07-01 00:00:00
abstract：:Phenylacetic acid derivatives, methyl 2-acetyl-3,5-dihydroxyphenylacetate (1) and methyl 2-acetyl-5-hydroxy-3-methoxyphenylacetate (3); curvulin or ethyl 2-acetyl-3,5-dihydroxyphenylacetate (4), a known metabolite of Curvularia siddiqui, and 4-epiradicinol (5) have been isolated from the culture mycelia of Curvularia ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2006.05.032
更新日期：2006-09-01 00:00:00
abstract：:Investigation of the extracts of a culture of Cladosporium tenuissimum, a known hyperparasite of several rust fungi, gave rise to the isolation of cladosporols B-E (2-5). Their structure and stereochemistry were elucidated on the basis of 1H and 13C NMR evidence and CD measures. Cladosporols 1-5 were active in inhibit...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.03.013
更新日期：2004-07-01 00:00:00
abstract：:Two biflavonoids namely, 2",3"-dihydroochnaflavone and 2",3"-dihydroochnaflavone 7"-O-methyl ether, and a flavonoid glycoside, 6-gamma,gamma-dimethylallyltaxifolin 7-O-beta-D-glucoside were isolated from the leaves of Ochna integerrima. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00409-x
更新日期：2001-02-01 00:00:00
